##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554253_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1106400
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25509219088937	34.0	31.0	34.0	30.0	34.0
2	32.4403226681128	34.0	31.0	34.0	30.0	34.0
3	32.527797360809835	34.0	31.0	34.0	30.0	34.0
4	36.05354211858279	37.0	35.0	37.0	35.0	37.0
5	35.88882682574114	37.0	35.0	37.0	35.0	37.0
6	35.87519793926247	37.0	35.0	37.0	35.0	37.0
7	36.03016088214027	37.0	35.0	37.0	35.0	37.0
8	36.14097975415763	37.0	35.0	37.0	35.0	37.0
9	37.765561279826464	39.0	38.0	39.0	35.0	39.0
10	37.24323662328272	39.0	37.0	39.0	34.0	39.0
11	37.249491142443965	39.0	37.0	39.0	34.0	39.0
12	37.26700560375994	39.0	37.0	39.0	34.0	39.0
13	37.24850054229935	39.0	37.0	39.0	34.0	39.0
14	38.499837310195225	40.0	38.0	41.0	34.0	41.0
15	38.495984273318875	40.0	38.0	41.0	34.0	41.0
16	38.49398861171367	40.0	38.0	41.0	34.0	41.0
17	38.447145697758494	40.0	38.0	41.0	34.0	41.0
18	38.38059291395517	40.0	38.0	41.0	34.0	41.0
19	38.37472071583514	40.0	38.0	41.0	34.0	41.0
20	38.305243130874906	40.0	38.0	41.0	34.0	41.0
21	38.25676518438178	40.0	38.0	41.0	34.0	41.0
22	38.20847342733189	40.0	37.0	41.0	34.0	41.0
23	38.117656362979034	40.0	37.0	41.0	33.0	41.0
24	38.10685737527115	40.0	37.0	41.0	33.0	41.0
25	38.04401572668113	40.0	37.0	41.0	33.0	41.0
26	37.97617498192336	40.0	37.0	41.0	33.0	41.0
27	37.91741232827187	40.0	37.0	41.0	33.0	41.0
28	37.82709237165582	40.0	37.0	41.0	33.0	41.0
29	37.76610177151121	40.0	37.0	41.0	33.0	41.0
30	37.69758676789588	40.0	37.0	41.0	33.0	41.0
31	37.6158251988431	40.0	37.0	41.0	33.0	41.0
32	37.55049349240781	40.0	36.0	41.0	33.0	41.0
33	37.496952277657265	40.0	36.0	41.0	32.0	41.0
34	37.415333514099785	40.0	36.0	41.0	32.0	41.0
35	37.35677422270427	40.0	36.0	41.0	32.0	41.0
36	37.28970986984815	39.0	36.0	41.0	32.0	41.0
37	37.24312545191612	39.0	36.0	41.0	32.0	41.0
38	37.20905820679682	39.0	36.0	41.0	31.0	41.0
39	37.15541214750542	39.0	35.0	41.0	31.0	41.0
40	37.05747288503254	39.0	35.0	41.0	31.0	41.0
41	36.98274403470716	39.0	35.0	41.0	31.0	41.0
42	36.87333152566884	39.0	35.0	41.0	31.0	41.0
43	36.776797722342735	39.0	35.0	41.0	31.0	41.0
44	36.69547722342733	39.0	35.0	41.0	31.0	41.0
45	36.54729302241504	39.0	35.0	41.0	30.0	41.0
46	36.40789587852495	39.0	35.0	40.0	30.0	41.0
47	36.30794739696312	39.0	35.0	40.0	30.0	41.0
48	36.194465835140996	38.0	35.0	40.0	30.0	41.0
49	36.10879880694143	38.0	35.0	40.0	29.0	41.0
50	36.00393438177874	38.0	35.0	40.0	29.0	41.0
51	34.927618402024585	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	3.0
10	7.0
11	14.0
12	13.0
13	15.0
14	21.0
15	39.0
16	69.0
17	140.0
18	237.0
19	424.0
20	722.0
21	1111.0
22	1615.0
23	2396.0
24	3399.0
25	4605.0
26	5844.0
27	7695.0
28	9759.0
29	12962.0
30	16749.0
31	22269.0
32	28569.0
33	38891.0
34	57994.0
35	74797.0
36	93936.0
37	138952.0
38	232755.0
39	350234.0
40	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.45480838756327	31.662147505422993	20.474421547360812	27.40862255965293
2	21.32962762111352	31.223427331887198	20.94911424439624	26.49783080260304
3	19.744396240057846	30.978488792480114	22.365238611713668	26.911876355748372
4	19.436460592913953	31.48201373825018	20.42190889370933	28.659616775126533
5	18.943691250903832	32.64940347071584	19.06679320318149	29.340112075198842
6	20.87762111352133	35.709146782357195	20.38123644251627	23.031995661605205
7	87.05296456977585	4.329537237888648	4.651663051337672	3.965835140997831
8	88.57302964569776	3.9937635574837307	4.101590744757773	3.3316160520607374
9	79.82474692697036	8.664045553145336	7.296547360809834	4.214660159074476
10	30.172812725958064	38.565798987707886	15.624909616775126	15.63647866955893
11	21.25677874186551	28.335412147505423	31.572306579898772	18.835502530730295
12	23.674349240780913	24.38304410701374	33.13042299349241	18.812183658712943
13	22.82809110629067	24.935737527114966	31.08740057845264	21.14877078814172
14	19.28515907447578	26.70480838756327	31.955712219812003	22.05432031814895
15	18.497469269703544	29.12111352133044	31.810104844540852	20.57131236442516
16	20.372198120028923	28.47152928416486	29.927331887201735	21.228940708604483
17	20.996294287780188	28.002982646420826	30.3191431670282	20.68157989877079
18	20.80748373101952	27.444956616052064	30.863702096890815	20.8838575560376
19	20.362255965292842	29.317335502530728	29.82601229211858	20.494396240057846
20	21.15274765003615	28.390093998553866	30.175162689804775	20.281995661605208
21	21.735809833694866	29.006778741865507	29.13982284887925	20.117588575560376
22	21.307483731019524	27.02051699204628	29.759309472161966	21.912689804772235
23	20.341015907447577	28.694866232827188	29.32131236442516	21.64280549530007
24	18.57781995661605	28.078543022415044	31.534887924801158	21.80874909616775
25	19.992317425885755	29.564352856109906	29.04781272595806	21.395516992046275
26	20.97677151120752	29.401934201012292	28.41422631959508	21.207067968185108
27	19.351138828633406	28.50515184381779	29.03452639190166	23.109182935647144
28	19.430766449746926	28.837400578452637	30.129338394793926	21.602494577006507
29	20.82203543022415	28.154013015184383	28.58161605206074	22.44233550253073
30	20.320950831525668	28.775126536514822	30.205260303687638	20.698662328271872
31	20.256417208966017	29.348246565437453	27.754880694143168	22.640455531453362
32	21.04410701373825	27.99936731742589	28.54103398409255	22.415491684743312
33	20.05531453362256	28.114244396240057	29.272866955892983	22.557574114244396
34	19.08459869848156	27.70797180043384	29.81941431670282	23.38801518438178
35	19.225777295733913	27.25469992769342	30.967823571945047	22.55169920462762
36	18.664226319595084	30.231561822125812	28.882321041214748	22.221890817064352
37	18.53154374548084	29.661966738973245	28.889190166305134	22.91729934924078
38	19.094360086767896	27.638738250180765	31.494125090383225	21.77277657266811
39	18.946041214750544	27.677241503976862	30.527476500361534	22.84924078091106
40	20.099240780911064	27.84815618221258	30.372830802603033	21.67977223427332
41	18.857104121475054	27.42172812725958	32.29799349240781	21.423174258857554
42	19.308116413593638	28.217010122921188	29.889190166305134	22.585683297180044
43	18.888467100506144	28.34336587129429	30.383767172812725	22.384399855386842
44	20.664226319595084	27.549168474331164	29.393438177874188	22.393167028199564
45	20.277747650036154	27.696583514099782	29.43103759942155	22.594631236442517
46	18.616503976861896	28.70824295010846	30.43709327548807	22.238159797541577
47	19.08658712942878	28.643167028199567	30.0381417208966	22.232104121475054
48	19.312454808387564	27.611080983369483	31.026934201012292	22.049530007230658
49	19.477946493130872	27.61596167751265	30.24972885032538	22.65636297903109
50	18.942606652205352	27.565527838033262	30.35538684020246	23.13647866955893
51	19.282537960954446	26.089389009399856	31.23436370209689	23.393709327548805
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3839.0
1	3111.5
2	2384.0
3	1768.5
4	1153.0
5	1239.0
6	1325.0
7	1553.0
8	1781.0
9	2122.0
10	2463.0
11	2767.0
12	3071.0
13	3273.5
14	3476.0
15	3681.0
16	3886.0
17	3989.0
18	4092.0
19	4170.5
20	4249.0
21	5219.5
22	6190.0
23	7759.5
24	9329.0
25	10220.5
26	14325.5
27	17539.0
28	21365.0
29	25191.0
30	29049.0
31	32907.0
32	37581.5
33	42256.0
34	46440.0
35	50624.0
36	54357.5
37	58091.0
38	58265.5
39	58440.0
40	59864.5
41	61289.0
42	63806.0
43	66323.0
44	70312.0
45	74301.0
46	78008.5
47	81716.0
48	86631.5
49	91547.0
50	87642.5
51	83738.0
52	77195.0
53	70652.0
54	61812.5
55	52973.0
56	47322.0
57	41671.0
58	37550.5
59	33430.0
60	31316.5
61	29203.0
62	25901.5
63	22600.0
64	19905.0
65	17210.0
66	14486.0
67	11762.0
68	10197.0
69	8632.0
70	7238.0
71	5844.0
72	5006.0
73	4168.0
74	3435.0
75	2106.5
76	1511.0
77	1123.0
78	735.0
79	577.0
80	419.0
81	352.0
82	285.0
83	206.5
84	128.0
85	106.5
86	85.0
87	58.0
88	31.0
89	26.0
90	21.0
91	16.5
92	12.0
93	10.5
94	9.0
95	5.5
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1106400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.871976983306002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.99893137777717	20.346245351673396
2	9.155933836168021	5.103879542846955
3	3.6026604963665205	3.012398113001803
4	2.031570668756813	2.2649556367819788
5	1.436227259122279	2.0015246554526414
6	1.1074475390411425	1.852005139102416
7	0.8747075790645782	1.7065880657567867
8	0.7072935106674499	1.577093475981189
9	0.6029485556765191	1.5124831439340185
>10	6.542234809082248	38.5174287288197
>50	0.7544452051582329	14.563413662089184
>100	0.1836454877178319	6.883468673823047
>500	0.0013024502674924242	0.2555651216972451
>1k	6.512251337462121E-4	0.4029506890396905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3122	0.28217642805495297	No Hit
CTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGCT	1318	0.11912509038322487	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7114967462039046E-4	0.0	0.0	0.1480477223427332	0.0
2	2.7114967462039046E-4	0.0	0.0	0.26816702819956617	0.0
3	2.7114967462039046E-4	0.0	0.0	0.3819595083152567	0.0
4	2.7114967462039046E-4	0.0	0.0	0.9762292118582792	0.0
5	2.7114967462039046E-4	0.0	0.0	1.108640636297903	0.0
6	2.7114967462039046E-4	0.0	0.0	2.0617317425885755	0.0
7	2.7114967462039046E-4	0.0	0.0	2.784255242227043	0.0
8	2.7114967462039046E-4	0.0	0.0	3.367859725234996	0.0
9	2.7114967462039046E-4	0.0	0.0	4.017715112075199	0.0
10	2.7114967462039046E-4	0.0	0.0	5.218094721619668	0.0
11	2.7114967462039046E-4	0.0	0.0	6.09002169197397	0.0
12	2.7114967462039046E-4	0.0	0.0	6.560556760665221	0.0
13	2.7114967462039046E-4	0.0	0.0	6.790943600867679	0.0
14	2.7114967462039046E-4	0.0	0.0	7.024132321041215	0.0
15	2.7114967462039046E-4	0.0	0.0	7.212310195227766	0.0
16	2.7114967462039046E-4	0.0	0.0	7.5460954446854664	0.0
17	2.7114967462039046E-4	0.0	0.0	7.95408532176428	0.0
18	2.7114967462039046E-4	0.0	0.0	8.40184381778742	0.0
19	2.7114967462039046E-4	0.0	0.0	8.642082429501084	0.0
20	2.7114967462039046E-4	0.0	0.0	8.90970715835141	0.0
21	2.7114967462039046E-4	0.0	0.0	9.215112075198842	0.0
22	2.7114967462039046E-4	0.0	0.0	9.555947216196675	0.0
23	2.7114967462039046E-4	0.0	0.0	9.889190166305134	0.0
24	2.7114967462039046E-4	0.0	0.0	10.159074475777295	0.0
25	2.7114967462039046E-4	0.0	0.0	10.394793926247289	0.0
26	2.7114967462039046E-4	0.0	0.0	10.629519161243673	0.0
27	2.7114967462039046E-4	0.0	0.0	10.867588575560376	0.0
28	2.7114967462039046E-4	0.0	0.0	11.123373101952277	0.0
29	2.7114967462039046E-4	0.0	0.0	11.39768618944324	0.0
30	2.7114967462039046E-4	0.0	0.0	11.75	0.0
31	2.7114967462039046E-4	0.0	0.0	12.024493853940708	0.0
32	2.7114967462039046E-4	0.0	0.0	12.318510484454086	0.0
33	2.7114967462039046E-4	0.0	0.0	12.597885032537961	0.0
34	2.7114967462039046E-4	0.0	0.0	12.860177151120752	2.7114967462039046E-4
35	3.6153289949385393E-4	0.0	0.0	13.157176428054953	2.7114967462039046E-4
36	3.6153289949385393E-4	0.0	0.0	13.441883586406362	2.7114967462039046E-4
37	3.6153289949385393E-4	0.0	0.0	13.713304410701374	2.7114967462039046E-4
38	3.6153289949385393E-4	0.0	0.0	13.997198120028923	2.7114967462039046E-4
39	3.6153289949385393E-4	0.0	0.0	14.289587852494577	2.7114967462039046E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGGT	20	7.033171E-4	45.000004	3
CGCGGAT	40	6.8139343E-9	45.000004	23
TGCGATG	20	7.033171E-4	45.000004	1
TATAGCG	20	7.033171E-4	45.000004	1
CGCGTTG	20	7.033171E-4	45.000004	1
TCGAACG	20	7.033171E-4	45.000004	1
TAGTACG	20	7.033171E-4	45.000004	1
TCCGACG	25	3.8907107E-5	45.0	1
GTGATCG	25	3.8907107E-5	45.0	15
CCATGCG	45	3.8562575E-10	45.0	1
AATTGCG	105	0.0	45.0	1
GCGGAAT	35	1.2118653E-7	45.0	5
CAGTACG	70	0.0	45.0	1
CCACGCG	70	0.0	41.785713	1
ATTGCGG	255	0.0	41.47059	2
AAACGCG	60	3.6379788E-12	41.249996	1
GCTAGCG	55	6.184564E-11	40.909092	1
TGTAGCG	50	1.0822987E-9	40.5	1
TACACCG	50	1.0822987E-9	40.5	1
CATTGCG	90	0.0	40.0	1
>>END_MODULE