FastQCFastQC Report
Sat 18 Jun 2016
SRR3554252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834837
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52940.6341357654248674No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10850.12996549026935797No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9600.11499250751943194No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAT253.889884E-545.00000445
CGACACG253.889884E-545.0000041
ATTCACG253.889884E-545.0000041
TGCGATA253.889884E-545.00000421
CGTAGCG502.1827873E-1145.0000041
GTATACG302.1647847E-645.0000041
TGTAACG253.889884E-545.0000041
TATACGG750.045.02
CGCTTAT351.2113924E-745.023
CTTACCG351.2113924E-745.01
TTAACGG900.042.52
TAACGCG556.002665E-1140.9090921
GTACGGG3400.040.3676453
CTTAACG451.9272193E-840.01
TAAACCG451.9272193E-840.01
TAACGTC451.9272193E-840.032
ACTTACG451.9272193E-840.01
CTTTACG451.9272193E-840.01
GTAACGG900.040.02
TAAGCGG1300.039.807692