##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554252_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	834837
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.284563333920275	34.0	31.0	34.0	30.0	34.0
2	32.468541763242406	34.0	31.0	34.0	30.0	34.0
3	32.56488751696439	34.0	31.0	34.0	31.0	34.0
4	36.081569216505734	37.0	35.0	37.0	35.0	37.0
5	35.92000114992508	37.0	35.0	37.0	35.0	37.0
6	35.893418715270165	37.0	35.0	37.0	35.0	37.0
7	35.96819498896192	37.0	35.0	37.0	35.0	37.0
8	36.06855350206088	37.0	35.0	37.0	35.0	37.0
9	37.74169568430724	39.0	38.0	39.0	35.0	39.0
10	37.312129194082196	39.0	37.0	39.0	34.0	39.0
11	37.22045381313957	39.0	37.0	39.0	34.0	39.0
12	37.09815688571541	39.0	37.0	39.0	33.0	39.0
13	37.08985227056299	39.0	37.0	39.0	33.0	39.0
14	38.31268738687911	40.0	38.0	41.0	34.0	41.0
15	38.309177719722534	40.0	38.0	41.0	33.0	41.0
16	38.302960937284766	40.0	38.0	41.0	33.0	41.0
17	38.233360524270005	40.0	37.0	41.0	33.0	41.0
18	38.169371985189926	40.0	37.0	41.0	33.0	41.0
19	38.14285782733636	40.0	37.0	41.0	34.0	41.0
20	38.08766980859737	40.0	37.0	41.0	33.0	41.0
21	38.05075601584501	40.0	37.0	41.0	33.0	41.0
22	37.99673948327638	40.0	37.0	41.0	33.0	41.0
23	37.91306566431531	40.0	37.0	41.0	33.0	41.0
24	37.85672173130803	40.0	37.0	41.0	33.0	41.0
25	37.77390915831474	40.0	36.0	41.0	33.0	41.0
26	37.659974342296756	40.0	36.0	41.0	33.0	41.0
27	37.59681350970309	40.0	36.0	41.0	33.0	41.0
28	37.53272914353341	40.0	36.0	41.0	33.0	41.0
29	37.440024819216205	40.0	36.0	41.0	32.0	41.0
30	37.37041362565387	39.0	36.0	41.0	32.0	41.0
31	37.28564618003275	39.0	35.0	41.0	32.0	41.0
32	37.20561139479923	39.0	35.0	41.0	32.0	41.0
33	37.093496095645015	39.0	35.0	41.0	31.0	41.0
34	36.95806965910711	39.0	35.0	41.0	31.0	41.0
35	36.881455900972284	39.0	35.0	41.0	31.0	41.0
36	36.76607888725584	39.0	35.0	41.0	31.0	41.0
37	36.72596207403361	39.0	35.0	41.0	31.0	41.0
38	36.638412049298246	39.0	35.0	41.0	31.0	41.0
39	36.568345677060314	39.0	35.0	41.0	30.0	41.0
40	36.48723044139155	39.0	35.0	41.0	30.0	41.0
41	36.38657486431483	39.0	35.0	40.0	30.0	41.0
42	36.263676621903436	38.0	35.0	40.0	30.0	41.0
43	36.156992323052286	38.0	35.0	40.0	30.0	41.0
44	36.04695407606515	38.0	35.0	40.0	30.0	41.0
45	35.926771333805284	38.0	35.0	40.0	29.0	41.0
46	35.81457697730215	38.0	35.0	40.0	29.0	41.0
47	35.655950802372196	38.0	34.0	40.0	28.0	41.0
48	35.57685512261675	38.0	34.0	40.0	28.0	41.0
49	35.411658802856124	38.0	34.0	40.0	28.0	41.0
50	35.25518753960354	37.0	34.0	40.0	27.0	41.0
51	34.20759142203807	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	13.0
12	13.0
13	21.0
14	33.0
15	58.0
16	82.0
17	144.0
18	256.0
19	489.0
20	792.0
21	1197.0
22	1706.0
23	2563.0
24	3346.0
25	4438.0
26	5678.0
27	6898.0
28	8634.0
29	11034.0
30	14082.0
31	18083.0
32	24085.0
33	33134.0
34	52263.0
35	64411.0
36	74270.0
37	105485.0
38	169728.0
39	231811.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.575469223333418	31.95126713358416	20.584018197564315	25.889245445518107
2	22.820742252679267	30.723722115814223	20.451297678468972	26.00423795303754
3	21.54863763824555	30.52823485303119	21.689982595404853	26.233144913318405
4	20.69637546011976	31.58460873200397	20.25089927734396	27.468116530532306
5	19.565136667397347	33.99166543888208	17.423880350295924	29.019317543424645
6	21.33158928030262	36.43920909111599	19.67210365616282	22.557097972418568
7	84.92687794144246	6.203725996811354	5.124593184058685	3.744802877687501
8	86.1130975268226	5.668411917535998	4.540886424535568	3.6776041311058325
9	78.41446893225863	9.233419218362387	7.424802686033321	4.927309163345659
10	36.757714380172416	32.90905889413143	15.117921222945318	15.215305502750837
11	26.861650837229305	28.507840452687173	26.22655680090844	18.403951909175085
12	26.887045015973175	25.49431805250606	28.656971360876433	18.96166557064433
13	24.881264246793087	25.995134379525588	27.82603071018654	21.29757066349479
14	21.655484843149022	27.874782742020297	28.997996015988747	21.47173639884193
15	21.764967293016483	28.552998968660948	29.360701550122958	20.321332188199612
16	24.213349432284385	28.631816749856558	27.211779065853577	19.94305475200548
17	23.462544185272094	28.28504246936827	27.850346834172417	20.402066511187215
18	23.54411699529369	28.208380797688648	27.530643706496	20.71685850052166
19	22.677121402141974	30.061077791233497	27.183150722835713	20.078650083788812
20	23.533695799299746	29.353514518402996	27.904249572072153	19.20854011022511
21	23.99426474868747	29.33782283248107	27.28939900842919	19.37851341040227
22	23.50614551103988	28.212094097410635	27.440326674548444	20.841433717001042
23	23.151106144073633	28.76908905570788	27.722297885695053	20.357506914523434
24	21.752629555230545	27.956355552041895	29.402985253408747	20.888029639318813
25	22.298125262775848	29.83983699812059	26.55716026002681	21.304877479076755
26	21.94524200532559	30.032090096629645	26.67466822864823	21.347999669396543
27	21.029135028754116	28.898695194391237	27.868194629610333	22.20397514724431
28	20.91749646937067	30.56812287907699	27.793329715860697	20.721050935691636
29	22.13809402314464	29.204862745661732	26.986345837570685	21.670697393622948
30	22.276683951477953	29.035847716380562	28.056854212259395	20.630614119882086
31	22.79115563876541	29.613445498941708	27.36713873486681	20.228260127426072
32	24.121714777854837	28.231858434640532	27.62515317361353	20.021273613891093
33	22.546916344148617	29.24271444605354	27.373487279552776	20.836881930245067
34	22.20038163138433	29.219356592963656	29.22810081488961	19.352160960762397
35	23.168714371787548	29.588530455645834	28.74465314786	18.49810202470662
36	22.306629916977805	32.51449085270538	26.650112536938348	18.52876669337847
37	22.512538375754787	31.295091137551402	26.84104801296541	19.351322473728406
38	22.677121402141974	30.244347100092593	27.825192223152545	19.253339274612888
39	23.035394933382207	29.80378205565877	26.56087355974879	20.599949451210236
40	23.3407239976187	29.57679163716989	27.053784151876354	20.028700213335057
41	21.60361843090328	28.91270990624517	28.682006188034308	20.80166547481724
42	22.01615405162924	29.140538811768046	27.010302609970573	21.833004526632145
43	21.084594956859842	29.985134822725872	27.59472807266568	21.3355421477486
44	21.757061558124523	28.85605213951945	27.468356098256308	21.918530204099724
45	22.020825622247216	29.258406131975462	27.500697740996145	21.220070504781173
46	20.97343553292439	30.204099722460793	27.95132462983792	20.871140114776896
47	20.881561310770845	29.57667185330789	28.49538293103923	21.04638390488203
48	21.28343616777886	29.137065079770064	29.24139682357155	20.338101928879528
49	21.355546052702504	29.951834909090035	27.897421891938183	20.795197146269274
50	20.507596093608694	29.2501410454975	29.38897054155482	20.853292319338983
51	20.884076771872834	28.09901813168319	29.19252500787579	21.824380088568187
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6497.0
1	5097.0
2	3697.0
3	2645.0
4	1593.0
5	1524.0
6	1455.0
7	1605.5
8	1756.0
9	2186.0
10	2616.0
11	3246.0
12	3876.0
13	3932.0
14	3988.0
15	4021.0
16	4054.0
17	3794.5
18	3535.0
19	3754.5
20	3974.0
21	4090.0
22	4206.0
23	4590.0
24	4974.0
25	6404.0
26	9954.0
27	12074.0
28	14660.0
29	17246.0
30	19729.0
31	22212.0
32	24686.5
33	27161.0
34	31047.5
35	34934.0
36	36260.0
37	37586.0
38	39899.0
39	42212.0
40	41655.0
41	41098.0
42	43936.0
43	46774.0
44	47799.5
45	48825.0
46	51079.5
47	53334.0
48	53844.5
49	54355.0
50	53372.0
51	52389.0
52	50268.0
53	48147.0
54	44630.5
55	41114.0
56	38838.5
57	36563.0
58	35381.0
59	34199.0
60	33540.0
61	32881.0
62	30798.5
63	28716.0
64	24688.5
65	20661.0
66	17974.5
67	15288.0
68	13187.0
69	11086.0
70	9156.5
71	7227.0
72	6466.0
73	5705.0
74	4632.5
75	2898.5
76	2237.0
77	1791.0
78	1345.0
79	1112.5
80	880.0
81	687.5
82	495.0
83	353.0
84	211.0
85	164.5
86	118.0
87	96.5
88	75.0
89	48.5
90	22.0
91	20.5
92	19.0
93	18.0
94	17.0
95	15.0
96	13.0
97	8.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	834837.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.634005775450163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.22969907531433	18.771705290310855
2	8.407860776210157	4.310543033931042
3	3.4501995895258624	2.6532730861308527
4	2.0896827582528354	2.1426775957564725
5	1.4521204361517417	1.861183182348148
6	1.0618787092498643	1.6332122979443149
7	0.9181803362837481	1.6475648030241663
8	0.7482054673800964	1.5343602617635825
9	0.5999079802120938	1.384024016654591
>10	6.925503197705394	38.43264637879615
>50	0.8554019038646483	15.092723282209427
>100	0.2576059490973574	9.295740555206011
>500	0.0028153655639055458	0.47306843165541246
>1k	4.6922759398425757E-4	0.13050578396799456
>5k	4.6922759398425757E-4	0.6367720003009798
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5294	0.6341357654248674	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1085	0.12996549026935797	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	960	0.11499250751943194	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3956772399881654E-4	0.0	0.0	0.1158309945534278	0.0
2	2.3956772399881654E-4	0.0	0.0	0.17452508693313784	0.0
3	2.3956772399881654E-4	0.0	0.0	0.24531734937478814	0.0
4	2.3956772399881654E-4	0.0	0.0	0.5501672781632821	0.0
5	2.3956772399881654E-4	0.0	0.0	0.6185638633649443	0.0
6	2.3956772399881654E-4	0.0	0.0	1.0437965734628436	0.0
7	2.3956772399881654E-4	0.0	0.0	1.3694888942392347	0.0
8	2.3956772399881654E-4	0.0	0.0	1.6834423965396839	0.0
9	2.3956772399881654E-4	0.0	0.0	2.095978017265646	0.0
10	2.3956772399881654E-4	0.0	0.0	2.68016391223676	0.0
11	2.3956772399881654E-4	0.0	0.0	3.213920801306123	0.0
12	2.3956772399881654E-4	0.0	0.0	3.44737954834297	0.0
13	2.3956772399881654E-4	0.0	0.0	3.565965571722384	0.0
14	2.3956772399881654E-4	0.0	0.0	3.6960508458537413	0.0
15	2.3956772399881654E-4	0.0	0.0	3.802898050757214	0.0
16	2.3956772399881654E-4	0.0	0.0	3.9921565527162786	0.0
17	2.3956772399881654E-4	0.0	0.0	4.206929017281218	0.0
18	2.3956772399881654E-4	0.0	0.0	4.468536971887925	0.0
19	2.3956772399881654E-4	0.0	0.0	4.614793067389203	0.0
20	2.3956772399881654E-4	0.0	0.0	4.775063874744411	0.0
21	2.3956772399881654E-4	0.0	0.0	4.961088212429492	0.0
22	2.3956772399881654E-4	0.0	0.0	5.1623251005884985	0.0
23	2.3956772399881654E-4	0.0	0.0	5.347870302825582	0.0
24	2.3956772399881654E-4	0.0	0.0	5.511495058316773	0.0
25	2.3956772399881654E-4	0.0	0.0	5.65990726333404	0.0
26	2.3956772399881654E-4	0.0	0.0	5.79765870463336	0.0
27	2.3956772399881654E-4	0.0	0.0	5.945112638754631	0.0
28	2.3956772399881654E-4	0.0	0.0	6.094602898529892	0.0
29	2.3956772399881654E-4	0.0	0.0	6.256910031539091	0.0
30	2.3956772399881654E-4	0.0	0.0	6.472161631552027	0.0
31	2.3956772399881654E-4	0.0	0.0	6.630036761667248	0.0
32	2.3956772399881654E-4	0.0	0.0	6.8068377419783745	0.0
33	2.3956772399881654E-4	0.0	0.0	6.977649529189531	0.0
34	2.3956772399881654E-4	0.0	0.0	7.152533967708666	0.0
35	2.3956772399881654E-4	0.0	0.0	7.341552901943733	0.0
36	2.3956772399881654E-4	0.0	0.0	7.527337671904815	0.0
37	2.3956772399881654E-4	0.0	0.0	7.713721361175894	0.0
38	2.3956772399881654E-4	0.0	0.0	7.923942038984856	0.0
39	2.3956772399881654E-4	0.0	0.0	8.14158931623778	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAT	25	3.889884E-5	45.000004	45
CGACACG	25	3.889884E-5	45.000004	1
ATTCACG	25	3.889884E-5	45.000004	1
TGCGATA	25	3.889884E-5	45.000004	21
CGTAGCG	50	2.1827873E-11	45.000004	1
GTATACG	30	2.1647847E-6	45.000004	1
TGTAACG	25	3.889884E-5	45.000004	1
TATACGG	75	0.0	45.0	2
CGCTTAT	35	1.2113924E-7	45.0	23
CTTACCG	35	1.2113924E-7	45.0	1
TTAACGG	90	0.0	42.5	2
TAACGCG	55	6.002665E-11	40.909092	1
GTACGGG	340	0.0	40.367645	3
CTTAACG	45	1.9272193E-8	40.0	1
TAAACCG	45	1.9272193E-8	40.0	1
TAACGTC	45	1.9272193E-8	40.0	32
ACTTACG	45	1.9272193E-8	40.0	1
CTTTACG	45	1.9272193E-8	40.0	1
GTAACGG	90	0.0	40.0	2
TAAGCGG	130	0.0	39.80769	2
>>END_MODULE