FastQCFastQC Report
Sat 18 Jun 2016
SRR3554248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410934
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62411.5187353686966765No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12350.30053487908033893No Hit
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT10020.2438347763874491Illumina PCR Primer Index 7 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9150.22266349340770047No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5850.1423586269327921No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAC207.0279895E-445.00000433
CGAACCG302.1620835E-645.0000041
ACGCCAT207.0279895E-445.00000414
GATCGTA207.0279895E-445.00000418
TCCGCAA207.0279895E-445.00000415
CGTATAG207.0279895E-445.0000041
CGGCCGT207.0279895E-445.00000422
GCGAAAG351.2093733E-745.0000041
ATAGCGG700.045.0000042
ATCGTCG302.1620835E-645.0000041
CGTTAGA207.0279895E-445.00000427
ACGGCAT207.0279895E-445.0000045
GTTAACG207.0279895E-445.00000422
CGCTATG207.0279895E-445.0000041
TCGTCGG302.1620835E-645.0000042
CCCCCGA207.0279895E-445.00000422
GGACGTT302.1620835E-645.0000048
CGTACTA207.0279895E-445.00000433
TCGTATT207.0279895E-445.00000420
CGCAGCG207.0279895E-445.0000041