##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554248_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410934
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.040753502995614	33.0	31.0	34.0	30.0	34.0
2	32.24469622859145	34.0	31.0	34.0	30.0	34.0
3	32.356373529569225	34.0	31.0	34.0	30.0	34.0
4	35.929197876057955	37.0	35.0	37.0	35.0	37.0
5	35.72408707967703	37.0	35.0	37.0	33.0	37.0
6	35.70279412265717	37.0	35.0	37.0	33.0	37.0
7	35.867455601142765	37.0	35.0	37.0	35.0	37.0
8	36.01902495291215	37.0	35.0	37.0	35.0	37.0
9	37.64331011792648	39.0	37.0	39.0	35.0	39.0
10	37.0691473569965	39.0	37.0	39.0	33.0	39.0
11	37.02343928708746	39.0	37.0	39.0	33.0	39.0
12	37.11533238914278	39.0	37.0	39.0	33.0	39.0
13	37.12319983257652	39.0	37.0	39.0	33.0	39.0
14	38.33862372059747	40.0	38.0	41.0	34.0	41.0
15	38.338728360271965	40.0	38.0	41.0	34.0	41.0
16	38.281483157879364	40.0	38.0	41.0	33.0	41.0
17	38.22368312186385	40.0	38.0	41.0	33.0	41.0
18	38.13004764755411	40.0	37.0	41.0	33.0	41.0
19	38.10589291711078	40.0	37.0	41.0	33.0	41.0
20	38.04450593039272	40.0	37.0	41.0	33.0	41.0
21	37.988864391848814	40.0	37.0	41.0	33.0	41.0
22	37.939598573006855	40.0	37.0	41.0	33.0	41.0
23	37.83314595531156	40.0	37.0	41.0	33.0	41.0
24	37.76756851465199	40.0	37.0	41.0	33.0	41.0
25	37.73007100896981	40.0	37.0	41.0	33.0	41.0
26	37.607097490107904	39.0	36.0	41.0	33.0	41.0
27	37.54702944998467	39.0	36.0	41.0	33.0	41.0
28	37.51614857860386	39.0	36.0	41.0	33.0	41.0
29	37.39559393965941	39.0	36.0	41.0	32.0	41.0
30	37.33006516861588	39.0	36.0	41.0	32.0	41.0
31	37.28161456584269	39.0	36.0	41.0	32.0	41.0
32	37.22309908647131	39.0	36.0	41.0	32.0	41.0
33	37.101162230431164	39.0	35.0	41.0	31.0	41.0
34	37.0355896567332	39.0	35.0	41.0	31.0	41.0
35	36.97906963161967	39.0	35.0	41.0	31.0	41.0
36	36.89897404449376	39.0	35.0	41.0	31.0	41.0
37	36.83312162050353	39.0	35.0	41.0	31.0	41.0
38	36.76426871468411	39.0	35.0	40.0	31.0	41.0
39	36.67601123294738	39.0	35.0	40.0	31.0	41.0
40	36.59246983700545	39.0	35.0	40.0	30.0	41.0
41	36.51374429957122	39.0	35.0	40.0	30.0	41.0
42	36.43222269269518	39.0	35.0	40.0	30.0	41.0
43	36.370409846836715	39.0	35.0	40.0	30.0	41.0
44	36.285043340293086	39.0	35.0	40.0	30.0	41.0
45	36.152552478013504	38.0	35.0	40.0	29.0	41.0
46	36.019178262202686	38.0	35.0	40.0	29.0	41.0
47	35.87545201905902	38.0	35.0	40.0	28.0	41.0
48	35.79089099466094	38.0	35.0	40.0	28.0	41.0
49	35.6688470654655	38.0	34.0	40.0	28.0	41.0
50	35.49820652464873	38.0	34.0	40.0	27.0	41.0
51	34.475533784013976	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	8.0
14	5.0
15	16.0
16	19.0
17	48.0
18	95.0
19	177.0
20	330.0
21	566.0
22	847.0
23	1201.0
24	1636.0
25	2048.0
26	2632.0
27	3208.0
28	4109.0
29	5442.0
30	7225.0
31	9364.0
32	11894.0
33	16079.0
34	22447.0
35	33058.0
36	40013.0
37	54094.0
38	84746.0
39	109577.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.125051711467048	34.48801997401043	21.944643178709963	25.442285135812565
2	19.52600660933386	32.78287997585987	23.14361916998837	24.5474942448179
3	18.68767247295186	34.018844875332775	22.31501895681545	24.97846369489991
4	18.778684654956756	33.53969250536583	21.167632758545167	26.51399008113225
5	17.219796853022626	37.77516584171667	17.98171969221335	27.023317613047347
6	19.702433967498433	38.544632471394436	19.59463076795787	22.158302793149264
7	85.95005524001421	6.813016202115181	3.810344240194289	3.4265843176763178
8	87.39992310200665	6.294928139311909	3.2394496439817586	3.065699114699684
9	78.52063835068405	12.123844704989123	6.060340589973086	3.2951763543537407
10	27.697635143356354	49.31838202728419	12.268880160804411	10.715102668555048
11	17.121240880530696	29.271610526264556	36.56353575026647	17.043612842938284
12	20.555855684854503	27.26593564903366	35.06037465870432	17.117834007407513
13	20.659278618950975	29.166484155606497	32.61764662938574	17.556590596056786
14	18.385190809229705	30.95363245679355	30.422403597658015	20.238773136318727
15	17.26968320946916	32.97974857276351	29.85515922264889	19.895408995118437
16	19.370750534149035	33.63240812393231	28.76690660787377	18.229934734044882
17	18.58984654470061	33.319705840840626	29.666321112392747	18.424126502066024
18	18.298558892668897	32.27209235546341	30.425810470781194	19.003538281086502
19	17.77365708361927	34.257325993955234	29.811599916288262	18.157417006137237
20	18.501024495417756	32.85685779224888	30.643850350664582	17.998267361668784
21	19.2568636326028	33.429699173103224	29.34266816569084	17.970769028603133
22	19.088223413005494	31.398229399368265	30.05397460419435	19.45957258343189
23	17.939133778173623	33.452817240724784	28.809492521913494	19.798556459188095
24	15.725152944268423	33.8017783877703	31.196007144699635	19.277061523261644
25	17.873186448432108	34.962548730453065	27.416811458774404	19.747453362340426
26	19.301883027444795	34.490453454812695	27.609299790233955	18.598363727508556
27	17.216876676059904	32.955170416660586	29.910885933020875	19.917066974258642
28	17.979042863330854	33.37056558960806	29.663644283510248	18.98674726355084
29	17.788257968432887	33.1437651788365	27.992816364671697	21.075160488058913
30	18.693999523037764	33.82562649963254	28.49654689074158	18.983827086588114
31	18.326057225734544	33.55891700370376	27.49541288868772	20.619612881873977
32	19.2018669664715	32.50497646824064	28.015934432293264	20.277222132994595
33	18.52097903799637	32.89506344084451	27.65942949476072	20.924528026398402
34	17.64297916453737	31.58439068074192	29.643689740931634	21.128940413789078
35	17.780227481785396	32.94154292416787	29.092749687297715	20.185479906749016
36	17.116373918926154	33.4051210170003	30.024529486486877	19.453975577586668
37	17.208846189412412	33.30534830410723	29.271610526264556	20.2141949802158
38	16.941406649242943	33.66136654547932	30.34039529462152	19.056831510656213
39	16.878866192624606	34.26438308828182	28.702662714693844	20.154088004399735
40	18.727094861948636	34.26852000564568	28.274856789654784	18.729528342750903
41	17.721093898290235	32.868051803939316	30.214827685224392	19.196026612546056
42	18.547017282580658	32.67580682055999	28.703392758934527	20.073783137924824
43	16.851611207639184	33.55794361138285	29.390121041335103	20.200324139642863
44	19.082139710999822	32.44024587890026	28.49703358690203	19.980580823197887
45	18.940754476387937	32.83738994583072	28.016421128453718	20.20543444932763
46	15.911557573722302	33.61853728335937	29.593803384485103	20.876101758433226
47	17.1952186969197	33.52241479166971	28.60167326139964	20.68069325001095
48	17.694568957545496	32.49475584887111	29.829120978064605	19.98155421551879
49	18.459898669859392	32.24191719351526	28.32961010770586	20.968574028919488
50	16.873512534859614	31.912423892887908	28.99078684168261	22.22327673056987
51	17.364832308837915	30.980644093698746	29.61156779434167	22.042955803121668
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7127.0
1	5290.5
2	3454.0
3	2194.5
4	935.0
5	899.0
6	863.0
7	1107.5
8	1352.0
9	1736.0
10	2120.0
11	2530.5
12	2941.0
13	3109.0
14	3277.0
15	3203.0
16	3129.0
17	3227.5
18	3326.0
19	3317.0
20	3308.0
21	3332.5
22	3357.0
23	4048.5
24	4740.0
25	5130.5
26	7124.5
27	8728.0
28	9397.0
29	10066.0
30	12352.5
31	14639.0
32	15936.5
33	17234.0
34	18771.0
35	20308.0
36	22419.0
37	24530.0
38	23560.0
39	22590.0
40	23247.5
41	23905.0
42	24720.5
43	25536.0
44	27377.0
45	29218.0
46	30493.0
47	31768.0
48	32908.5
49	34049.0
50	31228.5
51	28408.0
52	25838.5
53	23269.0
54	19564.0
55	15859.0
56	13191.5
57	10524.0
58	8938.5
59	7353.0
60	6653.5
61	5954.0
62	5089.0
63	4224.0
64	3470.0
65	2716.0
66	2179.5
67	1643.0
68	1352.0
69	1061.0
70	868.0
71	675.0
72	561.0
73	447.0
74	407.5
75	313.5
76	259.0
77	172.5
78	86.0
79	55.5
80	25.0
81	22.0
82	19.0
83	14.0
84	9.0
85	7.5
86	6.0
87	5.5
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	410934.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.1532943717298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.42263692494025	24.673555898929123
2	9.334364232989078	6.189298503784656
3	3.8949289919120935	3.873891822775393
4	2.3136854254430275	3.0682517597317434
5	1.588809276298709	2.633713081883304
6	1.141510436550202	2.2706898918810383
7	0.8549104620364009	1.9840168746555031
8	0.6560434298798298	1.740000076115622
9	0.5243334627396282	1.564504347523978
>10	4.606229285064787	31.14104762471872
>50	0.5118018404146308	11.737605395193047
>100	0.14706949437202038	6.690869024863398
>500	0.0014706949437202038	0.3656878680012788
>1k	0.0014706949437202038	0.5453625071459072
>5k	7.353474718601019E-4	1.5215053227973205
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6241	1.5187353686966765	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1235	0.30053487908033893	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	1002	0.2438347763874491	Illumina PCR Primer Index 7 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	915	0.22266349340770047	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	585	0.1423586269327921	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.8669616045399016E-4	0.0	0.0	0.30418510028374385	0.0
2	4.8669616045399016E-4	0.0	0.0	0.44338020217358504	0.0
3	4.8669616045399016E-4	0.0	0.0	0.5825753040634263	0.0
4	4.8669616045399016E-4	0.0	0.0	1.3182165505896324	0.0
5	4.8669616045399016E-4	0.0	0.0	1.4284532309324611	0.0
6	4.8669616045399016E-4	0.0	0.0	2.1504669849659557	0.0
7	4.8669616045399016E-4	0.0	0.0	2.731338852467793	0.0
8	4.8669616045399016E-4	0.0	0.0	3.3414124895968698	0.0
9	7.300442406809852E-4	0.0	0.0	4.18680372030545	0.0
10	7.300442406809852E-4	0.0	0.0	5.50769709977758	0.0
11	7.300442406809852E-4	0.0	0.0	6.623448047618353	0.0
12	7.300442406809852E-4	0.0	0.0	7.124988440966189	0.0
13	7.300442406809852E-4	0.0	0.0	7.350328763256386	0.0
14	7.300442406809852E-4	0.0	0.0	7.584186268354529	0.0
15	7.300442406809852E-4	0.0	0.0	7.7839750422208915	0.0
16	7.300442406809852E-4	0.0	0.0	8.201073651729962	0.0
17	7.300442406809852E-4	0.0	0.0	8.769291419059995	0.0
18	9.733923209079803E-4	0.0	0.0	9.430954849197194	0.0
19	9.733923209079803E-4	0.0	0.0	9.763124978707044	0.0
20	9.733923209079803E-4	0.0	0.0	10.087994665810081	0.0
21	9.733923209079803E-4	0.0	0.0	10.481001815376679	0.0
22	9.733923209079803E-4	0.0	0.0	10.92803223875367	0.0
23	9.733923209079803E-4	0.0	0.0	11.35705490419386	0.0
24	9.733923209079803E-4	0.0	0.0	11.674380800809862	0.0
25	9.733923209079803E-4	0.0	0.0	11.964695060520668	0.0
26	9.733923209079803E-4	0.0	0.0	12.244545352781712	0.0
27	9.733923209079803E-4	0.0	0.0	12.54678366842364	0.0
28	9.733923209079803E-4	0.0	0.0	12.852915553349199	0.0
29	9.733923209079803E-4	0.0	0.0	13.20114665615403	0.0
30	9.733923209079803E-4	0.0	0.0	13.62554570806991	0.0
31	9.733923209079803E-4	0.0	0.0	13.953335572135671	0.0
32	9.733923209079803E-4	0.0	0.0	14.29110270749074	0.0
33	9.733923209079803E-4	0.0	0.0	14.605265079063791	0.0
34	9.733923209079803E-4	0.0	0.0	14.930621462327284	0.0
35	9.733923209079803E-4	0.0	0.0	15.299537151951409	0.0
36	9.733923209079803E-4	0.0	0.0	15.617349744727864	0.0
37	9.733923209079803E-4	0.0	0.0	15.965337499452467	0.0
38	9.733923209079803E-4	0.0	0.0	16.316002083059566	0.0
39	9.733923209079803E-4	0.0	0.0	16.660826312741218	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAC	20	7.0279895E-4	45.000004	33
CGAACCG	30	2.1620835E-6	45.000004	1
ACGCCAT	20	7.0279895E-4	45.000004	14
GATCGTA	20	7.0279895E-4	45.000004	18
TCCGCAA	20	7.0279895E-4	45.000004	15
CGTATAG	20	7.0279895E-4	45.000004	1
CGGCCGT	20	7.0279895E-4	45.000004	22
GCGAAAG	35	1.2093733E-7	45.000004	1
ATAGCGG	70	0.0	45.000004	2
ATCGTCG	30	2.1620835E-6	45.000004	1
CGTTAGA	20	7.0279895E-4	45.000004	27
ACGGCAT	20	7.0279895E-4	45.000004	5
GTTAACG	20	7.0279895E-4	45.000004	22
CGCTATG	20	7.0279895E-4	45.000004	1
TCGTCGG	30	2.1620835E-6	45.000004	2
CCCCCGA	20	7.0279895E-4	45.000004	22
GGACGTT	30	2.1620835E-6	45.000004	8
CGTACTA	20	7.0279895E-4	45.000004	33
TCGTATT	20	7.0279895E-4	45.000004	20
CGCAGCG	20	7.0279895E-4	45.000004	1
>>END_MODULE