Basic Statistics
Measure | Value |
---|---|
Filename | SRR3554247_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 401650 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 41 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 6014 | 1.497323540395867 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1154 | 0.287314826341342 | No Hit |
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT | 1085 | 0.27013569027760487 | Illumina PCR Primer Index 7 (95% over 23bp) |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 890 | 0.2215859579235653 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 556 | 0.13842898045562058 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CACTACG | 30 | 2.1619617E-6 | 45.000004 | 1 |
TTCTCGG | 30 | 2.1619617E-6 | 45.000004 | 2 |
TCACGCG | 20 | 7.0278015E-4 | 45.0 | 1 |
AACGTTC | 25 | 3.8862592E-5 | 45.0 | 14 |
CTTCGGG | 45 | 3.8380676E-10 | 45.0 | 3 |
CGGGTCA | 40 | 6.7957444E-9 | 45.0 | 6 |
CGAACCG | 20 | 7.0278015E-4 | 45.0 | 1 |
TCCGCAA | 25 | 3.8862592E-5 | 45.0 | 15 |
CGTATAG | 20 | 7.0278015E-4 | 45.0 | 1 |
GCAACCG | 20 | 7.0278015E-4 | 45.0 | 1 |
TTTCGCG | 20 | 7.0278015E-4 | 45.0 | 1 |
CGCATGG | 50 | 2.1827873E-11 | 45.0 | 2 |
CGACCCG | 20 | 7.0278015E-4 | 45.0 | 1 |
ACTTCGG | 25 | 3.8862592E-5 | 45.0 | 2 |
CGATTGG | 20 | 7.0278015E-4 | 45.0 | 2 |
CCTCGTC | 70 | 0.0 | 45.0 | 36 |
TAACGCG | 25 | 3.8862592E-5 | 45.0 | 1 |
CGACAAG | 35 | 1.2092823E-7 | 45.0 | 1 |
TATGCGG | 25 | 3.8862592E-5 | 45.0 | 2 |
ACTTACG | 40 | 6.7957444E-9 | 45.0 | 1 |