FastQCFastQC Report
Sat 18 Jun 2016
SRR3554247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401650
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60141.497323540395867No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11540.287314826341342No Hit
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT10850.27013569027760487Illumina PCR Primer Index 7 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8900.2215859579235653No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5560.13842898045562058No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTACG302.1619617E-645.0000041
TTCTCGG302.1619617E-645.0000042
TCACGCG207.0278015E-445.01
AACGTTC253.8862592E-545.014
CTTCGGG453.8380676E-1045.03
CGGGTCA406.7957444E-945.06
CGAACCG207.0278015E-445.01
TCCGCAA253.8862592E-545.015
CGTATAG207.0278015E-445.01
GCAACCG207.0278015E-445.01
TTTCGCG207.0278015E-445.01
CGCATGG502.1827873E-1145.02
CGACCCG207.0278015E-445.01
ACTTCGG253.8862592E-545.02
CGATTGG207.0278015E-445.02
CCTCGTC700.045.036
TAACGCG253.8862592E-545.01
CGACAAG351.2092823E-745.01
TATGCGG253.8862592E-545.02
ACTTACG406.7957444E-945.01