##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554247_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	401650
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12526079920329	33.0	31.0	34.0	30.0	34.0
2	32.31534171542388	34.0	31.0	34.0	30.0	34.0
3	32.41402713805552	34.0	31.0	34.0	30.0	34.0
4	35.96367235154986	37.0	35.0	37.0	35.0	37.0
5	35.784299763475666	37.0	35.0	37.0	35.0	37.0
6	35.76766089879248	37.0	35.0	37.0	35.0	37.0
7	35.89386032615461	37.0	35.0	37.0	35.0	37.0
8	36.04951574754139	37.0	35.0	37.0	35.0	37.0
9	37.675232167309844	39.0	38.0	39.0	35.0	39.0
10	37.12975974106809	39.0	37.0	39.0	34.0	39.0
11	37.10219594174032	39.0	37.0	39.0	33.0	39.0
12	37.214940868915726	39.0	37.0	39.0	34.0	39.0
13	37.221389269264286	39.0	37.0	39.0	34.0	39.0
14	38.45286692393875	40.0	38.0	41.0	34.0	41.0
15	38.46470807917341	40.0	38.0	41.0	34.0	41.0
16	38.401102950329886	40.0	38.0	41.0	34.0	41.0
17	38.34506162081414	40.0	38.0	41.0	34.0	41.0
18	38.24707581227437	40.0	37.0	41.0	34.0	41.0
19	38.22817378314453	40.0	37.0	41.0	34.0	41.0
20	38.153496825594424	40.0	37.0	41.0	33.0	41.0
21	38.09830200423254	40.0	37.0	41.0	33.0	41.0
22	38.07166438441429	40.0	37.0	41.0	33.0	41.0
23	37.9417926055023	40.0	37.0	41.0	33.0	41.0
24	37.92371716668742	40.0	37.0	41.0	33.0	41.0
25	37.88082908004481	40.0	37.0	41.0	33.0	41.0
26	37.78583343707208	40.0	37.0	41.0	33.0	41.0
27	37.7434881115399	40.0	37.0	41.0	33.0	41.0
28	37.67351176397361	40.0	36.0	41.0	33.0	41.0
29	37.59914602265654	40.0	36.0	41.0	33.0	41.0
30	37.51094236275364	40.0	36.0	41.0	33.0	41.0
31	37.43218224822607	39.0	36.0	41.0	32.0	41.0
32	37.383567782895554	39.0	36.0	41.0	32.0	41.0
33	37.29142288061745	39.0	36.0	41.0	32.0	41.0
34	37.21821984314702	39.0	36.0	41.0	32.0	41.0
35	37.12228059255571	39.0	35.0	41.0	31.0	41.0
36	37.072647827710696	39.0	35.0	41.0	31.0	41.0
37	37.01980082161086	39.0	35.0	41.0	31.0	41.0
38	36.94434208888335	39.0	35.0	41.0	31.0	41.0
39	36.858727748039335	39.0	35.0	41.0	31.0	41.0
40	36.77147267521474	39.0	35.0	41.0	31.0	41.0
41	36.701038217353414	39.0	35.0	41.0	31.0	41.0
42	36.635954188970494	39.0	35.0	41.0	31.0	41.0
43	36.56033860326155	39.0	35.0	40.0	30.0	41.0
44	36.500017428109054	39.0	35.0	40.0	30.0	41.0
45	36.35035478650566	39.0	35.0	40.0	30.0	41.0
46	36.18084899788373	38.0	35.0	40.0	29.0	41.0
47	36.097525208514874	38.0	35.0	40.0	29.0	41.0
48	35.99715672849496	38.0	35.0	40.0	29.0	41.0
49	35.917692020415785	38.0	35.0	40.0	29.0	41.0
50	35.78935142537035	38.0	35.0	40.0	28.0	41.0
51	34.79761981824972	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	4.0
14	4.0
15	10.0
16	21.0
17	46.0
18	100.0
19	176.0
20	281.0
21	507.0
22	757.0
23	1026.0
24	1398.0
25	1822.0
26	2304.0
27	2954.0
28	3838.0
29	5084.0
30	6561.0
31	8402.0
32	10972.0
33	14714.0
34	20722.0
35	31377.0
36	37953.0
37	51574.0
38	82564.0
39	116424.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.150130710817876	34.394373210506664	21.94896053778165	25.506535540893815
2	19.663637495331756	32.72152371467696	23.00908751400473	24.605751275986556
3	18.700858956803188	34.09037719407444	22.34632142412548	24.86244242499689
4	18.76061247354662	33.20453130835304	21.329764720527823	26.705091497572514
5	17.337731856093612	37.66114776546745	17.92356529316569	27.077555085273247
6	19.749782148636875	38.34607245113905	19.622307979584217	22.28183742063986
7	85.87053404705588	6.713058633138304	3.8902029129839413	3.5262044068218596
8	87.29963898916967	6.252707581227438	3.289929042698867	3.157724386904021
9	78.41927050914975	12.105315573260302	6.101083032490974	3.3743308850989666
10	27.722395120129466	49.18311963151998	12.318187476658784	10.776297771691771
11	17.207767957176646	29.153989792107556	36.714303498070464	16.923938752645338
12	20.609983816755882	27.150006224324663	35.084277355906885	17.155732603012574
13	20.495456242997633	29.151251089256814	32.68616955060376	17.667123117141788
14	18.35827212747417	30.901282210880122	30.423752022905514	20.316693638740198
15	17.189592929167187	32.884849993775674	30.21610855222208	19.709448524835054
16	19.276982447404457	33.64446657537657	28.943358645586954	18.13519233163202
17	18.676957550105815	33.29316569152247	29.772936636374954	18.256940121996763
18	18.29702477281215	32.157350927424375	30.419270509149758	19.126353790613717
19	17.780156852981452	34.11054400597535	29.89592929167185	18.213369849371343
20	18.33536661272252	32.969500809162206	30.779036474542515	17.916096103572762
21	19.24536287812772	33.53865305614341	29.07282459853106	18.14315946719781
22	18.984439188348066	31.28320677206523	30.30524088136437	19.427113158222333
23	18.035105191086767	33.450267645960416	28.827088260923688	19.687538902029132
24	15.698991659404953	33.857836424747916	30.95755010581352	19.485621810033614
25	18.00995891945724	34.96352545748786	27.318311963152	19.7082036599029
26	19.148512386406075	34.62467322295531	27.576496950080916	18.6503174405577
27	17.17291173907631	33.08079173409685	29.776422258184986	19.969874268641853
28	18.095605626789492	33.203286443420886	29.821984314701854	18.87912361508776
29	17.7291173907631	33.22444914726752	27.854350802937883	21.192082659031495
30	18.708826092368977	33.84862442424997	28.45537159218225	18.987177891198805
31	18.525830947342214	33.49159716170795	27.373334993153243	20.609236897796592
32	19.259056392381428	32.3938752645338	28.062990165567037	20.28407817751774
33	18.38964272376447	33.04643346196938	27.730113282708825	20.833810531557326
34	17.708452632889333	31.549856840532804	29.655670359765967	21.0860201668119
35	17.79285447528943	32.84127972115026	29.031993028756382	20.333872774803936
36	17.00684675712685	33.53018797460475	29.971368106560437	19.491597161707954
37	17.207518984190216	33.190837794099345	29.114652060251462	20.48699116145898
38	16.940868915722643	33.5956678700361	30.446408564670733	19.01705464957052
39	16.795219718660526	34.26465828457612	28.856467073322545	20.083654923440807
40	18.71729117390763	34.162579360139425	28.42250715797336	18.697622307979582
41	17.727125606871656	32.93364869911615	30.300510394622183	19.038715299390017
42	18.52508402838292	32.58458857213992	28.7588696626416	20.131457736835554
43	16.79870534047056	33.53018797460475	29.376571641976845	20.29453504294784
44	18.999377567533923	32.339101207518986	28.517863811776422	20.143657413170672
45	19.006348811153988	32.79895431345699	27.960662268143903	20.234034607245114
46	15.883729615336737	33.66811900908752	29.62031619569277	20.827835179882985
47	17.34943358645587	33.39723639985061	28.518361757749283	20.73496825594423
48	17.7677082036599	32.423005103946224	29.85186107307357	19.957425619320304
49	18.423752022905514	32.33138304493963	28.29802066475787	20.946844267396987
50	16.87588696626416	31.79409934022159	28.959043943732105	22.370969749782148
51	17.342462342835802	31.02925432590564	29.5924312212125	22.03585211004606
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6850.0
1	5099.5
2	3349.0
3	2164.0
4	979.0
5	915.0
6	851.0
7	1072.5
8	1294.0
9	1696.0
10	2098.0
11	2517.5
12	2937.0
13	3075.0
14	3213.0
15	3120.5
16	3028.0
17	3100.0
18	3172.0
19	3187.0
20	3202.0
21	3253.0
22	3304.0
23	3928.5
24	4553.0
25	4938.0
26	6861.0
27	8399.0
28	9223.0
29	10047.0
30	12035.0
31	14023.0
32	15435.5
33	16848.0
34	18470.0
35	20092.0
36	22106.5
37	24121.0
38	23234.5
39	22348.0
40	22870.5
41	23393.0
42	23980.0
43	24567.0
44	26557.5
45	28548.0
46	29944.0
47	31340.0
48	32213.0
49	33086.0
50	30597.0
51	28108.0
52	25253.0
53	22398.0
54	18947.5
55	15497.0
56	12846.0
57	10195.0
58	8700.0
59	7205.0
60	6542.0
61	5879.0
62	5022.0
63	4165.0
64	3397.5
65	2630.0
66	2161.0
67	1692.0
68	1337.0
69	982.0
70	832.5
71	683.0
72	554.5
73	426.0
74	422.5
75	335.5
76	252.0
77	163.0
78	74.0
79	53.5
80	33.0
81	31.5
82	30.0
83	18.5
84	7.0
85	6.0
86	5.0
87	4.5
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	401650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.84414025397982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.72673274489374	24.21491150740977
2	9.642413826716863	6.3339358422320595
3	4.013280622703717	3.954382549519812
4	2.345869220908577	3.0819223083606277
5	1.5848049182462551	2.602577750503852
6	1.1738227929476763	2.313192026693508
7	0.8779048350323676	2.018382067201507
8	0.7153829661713969	1.8796910780993172
9	0.5780356465078142	1.7086575461132298
>10	4.678016525581581	31.28614671729379
>50	0.5256788330296863	12.0208027308578
>100	0.13426429068723594	6.168979593748471
>500	0.0015171106292343043	0.3602578446977218
>1k	0.0015171106292343043	0.557826635047164
>5k	7.585553146171522E-4	1.4983338022213684
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6014	1.497323540395867	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1154	0.287314826341342	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	1085	0.27013569027760487	Illumina PCR Primer Index 7 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	890	0.2215859579235653	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	556	0.13842898045562058	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.313954935889456	0.0
2	0.0	0.0	0.0	0.4561185111415411	0.0
3	0.0	0.0	0.0	0.6002738702850741	0.0
4	2.4897298643097225E-4	0.0	0.0	1.3780654798954313	0.0
5	2.4897298643097225E-4	0.0	0.0	1.4903522967757998	0.0
6	2.4897298643097225E-4	0.0	0.0	2.2350304991908376	0.0
7	2.4897298643097225E-4	0.0	0.0	2.843271505041703	0.0
8	2.4897298643097225E-4	0.0	0.0	3.4654549981327025	0.0
9	2.4897298643097225E-4	0.0	0.0	4.321922071455247	0.0
10	2.4897298643097225E-4	0.0	0.0	5.676833063612598	0.0
11	2.4897298643097225E-4	0.0	0.0	6.740445661645712	0.0
12	2.4897298643097225E-4	0.0	0.0	7.265280717042201	0.0
13	2.4897298643097225E-4	0.0	0.0	7.493837918585833	0.0
14	2.4897298643097225E-4	0.0	0.0	7.73882733723391	0.0
15	2.4897298643097225E-4	0.0	0.0	7.949956429727375	0.0
16	2.4897298643097225E-4	0.0	0.0	8.379683804307232	0.0
17	2.4897298643097225E-4	0.0	0.0	8.96003983567783	0.0
18	2.4897298643097225E-4	0.0	0.0	9.61832441180132	0.0
19	2.4897298643097225E-4	0.0	0.0	9.939250591310843	0.0
20	2.4897298643097225E-4	0.0	0.0	10.260425743806797	0.0
21	2.4897298643097225E-4	0.0	0.0	10.649321548611976	0.0
22	2.4897298643097225E-4	0.0	0.0	11.108427735590688	0.0
23	2.4897298643097225E-4	0.0	0.0	11.56728494958297	0.0
24	2.4897298643097225E-4	0.0	0.0	11.889953939997511	0.0
25	2.4897298643097225E-4	0.0	0.0	12.188721523714676	0.0
26	2.4897298643097225E-4	0.0	0.0	12.472052782273124	0.0
27	2.4897298643097225E-4	0.0	0.0	12.772563176895307	0.0
28	2.4897298643097225E-4	0.0	0.0	13.085024274866177	0.0
29	2.4897298643097225E-4	0.0	0.0	13.434582347815262	0.0
30	2.4897298643097225E-4	0.0	0.0	13.880990912485995	0.0
31	2.4897298643097225E-4	0.0	0.0	14.210133200547741	0.0
32	2.4897298643097225E-4	0.0	0.0	14.532304244989419	0.0
33	2.4897298643097225E-4	0.0	0.0	14.863189343956181	0.0
34	2.4897298643097225E-4	0.0	0.0	15.185111415411427	0.0
35	2.4897298643097225E-4	0.0	0.0	15.542387650939872	0.0
36	2.4897298643097225E-4	0.0	0.0	15.875264533798083	0.0
37	2.4897298643097225E-4	0.0	0.0	16.2210880119507	0.0
38	2.4897298643097225E-4	0.0	0.0	16.592057761732853	0.0
39	2.4897298643097225E-4	0.0	0.0	16.956056267894933	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTACG	30	2.1619617E-6	45.000004	1
TTCTCGG	30	2.1619617E-6	45.000004	2
TCACGCG	20	7.0278015E-4	45.0	1
AACGTTC	25	3.8862592E-5	45.0	14
CTTCGGG	45	3.8380676E-10	45.0	3
CGGGTCA	40	6.7957444E-9	45.0	6
CGAACCG	20	7.0278015E-4	45.0	1
TCCGCAA	25	3.8862592E-5	45.0	15
CGTATAG	20	7.0278015E-4	45.0	1
GCAACCG	20	7.0278015E-4	45.0	1
TTTCGCG	20	7.0278015E-4	45.0	1
CGCATGG	50	2.1827873E-11	45.0	2
CGACCCG	20	7.0278015E-4	45.0	1
ACTTCGG	25	3.8862592E-5	45.0	2
CGATTGG	20	7.0278015E-4	45.0	2
CCTCGTC	70	0.0	45.0	36
TAACGCG	25	3.8862592E-5	45.0	1
CGACAAG	35	1.2092823E-7	45.0	1
TATGCGG	25	3.8862592E-5	45.0	2
ACTTACG	40	6.7957444E-9	45.0	1
>>END_MODULE