FastQCFastQC Report
Sat 18 Jun 2016
SRR3554243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443984
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58081.3081552488377959No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10910.24572957584057084No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9620.21667447475584703No Hit
CTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGCT8810.19843057407474143Illumina Single End Adapter 1 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5830.13131103823561208No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG253.886924E-545.0000041
GCGCGAC253.886924E-545.0000049
CCGGCCG253.886924E-545.0000041
TATGGTC253.886924E-545.00000415
TTTCGCA253.886924E-545.00000422
CGTTGGG502.1827873E-1145.0000043
ACTCTCG253.886924E-545.00000433
GCGCAAT253.886924E-545.00000419
CGCCTGA253.886924E-545.00000436
ATCCGAG253.886924E-545.0000041
GTTACGA253.886924E-545.00000435
TATCTCG253.886924E-545.0000041
AACATCG253.886924E-545.0000041
TGTTCCG253.886924E-545.00000445
GCGGGCC253.886924E-545.0000045
CGTAACA253.886924E-545.00000432
ACCGTTG253.886924E-545.0000041
CACACGT253.886924E-545.00000436
ACGTAAC253.886924E-545.00000431
GGCGCGA253.886924E-545.0000048