##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554240_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	280605
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13409953493345	33.0	31.0	34.0	30.0	34.0
2	32.334609148090735	34.0	31.0	34.0	30.0	34.0
3	32.435950891823026	34.0	31.0	34.0	30.0	34.0
4	35.98756615170792	37.0	35.0	37.0	35.0	37.0
5	35.80012116676467	37.0	35.0	37.0	35.0	37.0
6	35.765720496783736	37.0	35.0	37.0	35.0	37.0
7	35.75926658470092	37.0	35.0	37.0	35.0	37.0
8	35.88991999429803	37.0	35.0	37.0	35.0	37.0
9	37.54941287575061	39.0	37.0	39.0	35.0	39.0
10	37.18573083159602	39.0	37.0	39.0	34.0	39.0
11	37.12575684681313	39.0	37.0	39.0	34.0	39.0
12	37.064150674435595	39.0	37.0	39.0	33.0	39.0
13	37.03615402434026	39.0	37.0	39.0	33.0	39.0
14	38.19923379839989	40.0	38.0	41.0	33.0	41.0
15	38.23754387840559	40.0	38.0	41.0	33.0	41.0
16	38.230986618199964	40.0	37.0	41.0	33.0	41.0
17	38.160018531387536	40.0	37.0	41.0	33.0	41.0
18	38.054247073288074	40.0	37.0	41.0	33.0	41.0
19	37.93294845066909	40.0	36.0	41.0	33.0	41.0
20	37.84667058676788	40.0	36.0	41.0	33.0	41.0
21	37.76478323622174	40.0	36.0	41.0	33.0	41.0
22	37.72742110796315	39.0	36.0	41.0	33.0	41.0
23	37.63264018816486	39.0	36.0	41.0	33.0	41.0
24	37.54207159530301	39.0	36.0	41.0	33.0	41.0
25	37.49180164287878	39.0	36.0	41.0	33.0	41.0
26	37.37095561376312	39.0	35.0	41.0	32.0	41.0
27	37.29171611339784	39.0	35.0	41.0	32.0	41.0
28	37.21126138165749	39.0	35.0	41.0	32.0	41.0
29	37.113871812690434	39.0	35.0	41.0	32.0	41.0
30	37.022993175460165	39.0	35.0	41.0	31.0	41.0
31	36.88582527039789	39.0	35.0	41.0	31.0	41.0
32	36.81962188841966	39.0	35.0	41.0	31.0	41.0
33	36.69797402042016	39.0	35.0	41.0	31.0	41.0
34	36.51929580727357	39.0	35.0	40.0	30.0	41.0
35	36.48920724862351	39.0	35.0	40.0	30.0	41.0
36	36.324848096078114	38.0	35.0	40.0	30.0	41.0
37	36.19388820584095	38.0	35.0	40.0	30.0	41.0
38	36.110664457155075	38.0	35.0	40.0	30.0	41.0
39	36.023474278790474	38.0	35.0	40.0	30.0	41.0
40	35.94668305981718	38.0	35.0	40.0	30.0	41.0
41	35.83383403716969	38.0	35.0	40.0	29.0	41.0
42	35.721412661926905	38.0	35.0	40.0	29.0	41.0
43	35.65387288180895	38.0	35.0	40.0	29.0	41.0
44	35.561547370859394	38.0	34.0	40.0	28.0	41.0
45	35.39666078651485	38.0	34.0	40.0	27.0	41.0
46	35.25340246966376	38.0	34.0	40.0	27.0	41.0
47	35.093911370075375	37.0	34.0	40.0	26.0	41.0
48	34.94509363696299	37.0	34.0	40.0	26.0	41.0
49	34.80509969530122	37.0	33.0	40.0	26.0	41.0
50	34.647140998913066	37.0	33.0	40.0	25.0	41.0
51	33.53575310489835	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	2.0
13	5.0
14	2.0
15	10.0
16	32.0
17	50.0
18	98.0
19	193.0
20	333.0
21	519.0
22	801.0
23	1119.0
24	1462.0
25	1738.0
26	2182.0
27	2561.0
28	3390.0
29	4238.0
30	5420.0
31	6888.0
32	9022.0
33	12224.0
34	18379.0
35	26202.0
36	27537.0
37	36460.0
38	53022.0
39	66681.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.61117941590492	34.7370859393097	20.684948593218223	23.966786051567148
2	21.521712015110207	33.863259742342436	21.296840754797667	23.318187487749682
3	20.408046898665383	33.528625648153096	22.1350296680387	23.928297785142817
4	20.50890041161063	33.33511519751964	20.017105896188593	26.138878494681133
5	17.98542435095597	36.32258869228988	18.011083195238857	27.680903761515296
6	20.22202027761444	38.215641203827445	19.896295504356658	21.66604301420146
7	80.1920849592844	9.469539031735001	5.678088416100925	4.660287592879671
8	81.54238163967142	8.61032412109549	5.1064663851321255	4.74082785410096
9	73.30482350635235	13.103472853299122	8.756793357210313	4.834910283138219
10	34.16439478982912	36.18253416724577	14.259902710215428	15.393168332709681
11	23.724096149391492	29.14131964861638	28.017676092728212	19.11690810926391
12	23.820673188289586	25.604675611624884	31.665864827782826	18.908786372302703
13	22.336736693929186	28.390798453341887	29.31095311915326	19.96151173357567
14	18.541365977085228	30.166604301420147	30.975214269168404	20.316815452326225
15	18.3703070151993	30.254984765061206	29.96311541134335	21.411592808396144
16	20.18567024821368	28.99021756561715	28.757862475722103	22.06624971044707
17	19.50321626485629	30.535806560823936	28.645961404821723	21.31501576949805
18	20.028509826980986	29.578589119937277	29.403610056841465	20.989290996240268
19	19.313269542595464	33.404964273623065	28.203702713779155	19.078063470002316
20	21.109388642397676	31.063951105646726	28.752873256000427	19.07378699595517
21	20.062365246520912	31.860444396928067	28.257158639368505	19.820031717182516
22	19.389176956932342	29.42677429126352	29.865112881096202	21.318935870707932
23	19.9176778745924	31.36829350866877	26.695176493647654	22.01885212309118
24	18.100532777391706	31.074285917927334	29.747866217636894	21.077315087044067
25	18.679282265105755	32.56214251349762	27.238288697635465	21.520286523761158
26	20.583025961761194	31.24570125265052	26.961743375919887	21.209529409668395
27	17.95798364248677	30.93244952869692	27.388321662122912	23.721245166693393
28	18.657899894870013	30.46275012918515	29.96026442864525	20.919085547299584
29	19.879902353842592	30.68975962652127	27.64954295183621	21.780795067799932
30	21.259421606885123	30.917838242369168	27.676270914630884	20.14646923611482
31	20.898772295575633	31.599579480052032	26.77963685607883	20.722011368293508
32	21.491063951105644	30.74856114466955	26.54122342795032	21.21915147627448
33	21.195274496177902	29.562908715097734	28.614600595142637	20.627216193581724
34	19.167156679317902	29.003403360595854	30.242511715757026	21.58692824432922
35	20.681741237682864	31.31911405712657	27.977049589280305	20.022095115910265
36	21.611874342937583	30.526897239892374	28.923575845049086	18.937652572120953
37	20.52422444361291	32.72678676431283	27.555460522798953	19.193528269275316
38	21.077671459881326	31.708273195417046	29.329128133853636	17.88492721084799
39	21.413018299745193	30.74749202615777	27.417900607615685	20.421589066481353
40	21.51921740524937	31.559665722278645	26.85411877906666	20.06699809340532
41	18.200673544662425	30.65412234279503	30.182997451934213	20.96220666060833
42	19.378842144651735	31.22431888241478	27.826660251955598	21.570178720977886
43	18.682845993478377	29.54829742876998	29.116017177170754	22.652839400580888
44	20.126868730065397	29.868676609468825	27.92288091801643	22.08157374244935
45	21.02813563550186	31.149124213752426	27.372997630120633	20.449742520625076
46	18.151850465957484	30.96416671121327	30.518344291798076	20.365638531031166
47	19.336077404180255	29.860480034211793	29.305607526594322	21.497835035013633
48	18.96865700896278	29.500899841414086	31.6925927905775	19.837850359045632
49	19.42659610484489	29.810944209832325	29.129202972149464	21.63325671317332
50	19.49074321555211	28.960994992961638	28.79777623349548	22.75048555799077
51	19.65788207622815	27.97740596211757	30.307727944976033	22.05698401667825
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6747.0
1	5134.0
2	3521.0
3	2215.0
4	909.0
5	783.5
6	658.0
7	686.5
8	715.0
9	966.5
10	1218.0
11	1465.0
12	1712.0
13	1862.0
14	2012.0
15	2143.5
16	2275.0
17	2287.5
18	2300.0
19	1993.0
20	1686.0
21	1923.0
22	2160.0
23	2399.0
24	2638.0
25	2916.5
26	3637.5
27	4080.0
28	4365.5
29	4651.0
30	5649.5
31	6648.0
32	7699.0
33	8750.0
34	9847.5
35	10945.0
36	12043.5
37	13142.0
38	12569.0
39	11996.0
40	12995.5
41	13995.0
42	14256.0
43	14517.0
44	15851.0
45	17185.0
46	18245.0
47	19305.0
48	20538.5
49	21772.0
50	20896.5
51	20021.0
52	17414.0
53	14807.0
54	13619.0
55	12431.0
56	11328.5
57	10226.0
58	9706.0
59	9186.0
60	9121.5
61	9057.0
62	8099.0
63	7141.0
64	6526.0
65	5911.0
66	5025.0
67	4139.0
68	3428.5
69	2718.0
70	2422.5
71	2127.0
72	1933.0
73	1739.0
74	1422.5
75	798.5
76	491.0
77	452.5
78	414.0
79	294.0
80	174.0
81	109.5
82	45.0
83	47.5
84	50.0
85	46.5
86	43.0
87	35.5
88	28.0
89	17.5
90	7.0
91	7.5
92	8.0
93	6.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	280605.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.19367081841022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.17301298968366	23.761158924466777
2	8.730621151363517	5.4468024447176635
3	3.462773188927352	3.240498209226493
4	1.8816190835246942	2.3477842518843213
5	1.2772617701157305	1.992124160296502
6	0.8716911722703956	1.6314748489870103
7	0.7345968856747895	1.6040341405178098
8	0.566656384595172	1.4140874182569805
9	0.46383566964846745	1.3021863473566047
>10	5.0370725800002285	32.82229468469913
>50	0.6089271229621506	13.011528661285437
>100	0.186219739292365	7.9645765399761235
>500	0.0022849047765934353	0.5252935621246948
>1k	0.0022849047765934353	0.8111045776090946
>5k	0.0011424523882967177	2.1250512285953564
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5963	2.1250512285953564	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1142	0.4069777801535967	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1134	0.40412679745549795	No Hit
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT	919	0.32750663744409403	TruSeq Adapter, Index 15 (95% over 22bp)
TTGTGCGGGGTTGTGCGGGGTTGATTGTTGCATCTTTATCTTTGTAGTTCA	555	0.19778692468060086	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	477	0.16998984337413803	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGT	335	0.1193849004828852	No Hit
AATCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCT	308	0.10976283387680191	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC	295	0.10512998699239146	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40091944192013684	0.0
2	0.0	0.0	0.0	0.575185759341423	0.0
3	0.0	0.0	0.0	0.7754672938828603	0.0
4	0.0	0.0	0.0	1.752641613656207	0.0
5	0.0	0.0	0.0	1.8937652572120953	0.0
6	0.0	0.0	0.0	2.7957449083230874	0.0
7	0.0	0.0	0.0	3.4357905240462574	0.0
8	0.0	0.0	0.0	3.9906630316637264	0.0
9	0.0	0.0	0.0	4.863063737281944	0.0
10	0.0	0.0	0.0	6.245077600185314	0.0
11	0.0	0.0	0.0	7.522674221770816	0.0
12	0.0	0.0	0.0	8.108194793392848	0.0
13	0.0	0.0	0.0	8.354804796778389	0.0
14	0.0	0.0	0.0	8.61174961244454	0.0
15	0.0	0.0	0.0	8.858359615830082	0.0
16	0.0	0.0	0.0	9.354786978136527	0.0
17	0.0	0.0	0.0	9.914292332638407	0.0
18	0.0	0.0	0.0	10.583204148179826	0.0
19	0.0	0.0	0.0	10.890397533899966	0.0
20	0.0	0.0	0.0	11.229664474973717	0.0
21	0.0	0.0	0.0	11.584611820887012	0.0
22	0.0	0.0	0.0	11.985887635644412	0.0
23	0.0	0.0	0.0	12.372908536911316	0.0
24	0.0	0.0	0.0	12.667628873327274	0.0
25	0.0	0.0	0.0	12.922791824807113	0.0
26	0.0	0.0	0.0	13.1547905418649	0.0
27	0.0	0.0	0.0	13.423139288323444	0.0
28	0.0	0.0	0.0	13.689706170595677	0.0
29	0.0	0.0	0.0	13.995830437804031	0.0
30	0.0	0.0	0.0	14.349352292368275	0.0
31	0.0	0.0	0.0	14.616275547477771	0.0
32	0.0	0.0	0.0	14.887118903797152	0.0
33	0.0	0.0	0.0	15.17399903779334	0.0
34	0.0	0.0	0.0	15.449831613834393	0.0
35	0.0	0.0	0.0	15.769141676021453	0.0
36	0.0	0.0	0.0	16.048894353272395	0.0
37	0.0	0.0	0.0	16.346109299549187	0.0
38	0.0	0.0	0.0	16.640473263127884	0.0
39	0.0	0.0	0.0	16.96940539192103	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	30	2.159617E-6	45.000004	1
CAGGTCG	30	2.159617E-6	45.000004	1
CGGGTAA	25	3.8832448E-5	45.000004	6
TCGCATT	25	3.8832448E-5	45.000004	22
GAACTAC	30	2.159617E-6	45.000004	33
TAGCGGA	25	3.8832448E-5	45.000004	3
GACCGAT	50	2.1827873E-11	45.000004	9
TCCCTAG	25	3.8832448E-5	45.000004	42
CGACAGG	30	2.159617E-6	45.000004	2
GCACCGG	25	3.8832448E-5	45.000004	2
TACGCGG	60	0.0	45.000004	2
ATTCACG	30	2.159617E-6	45.000004	1
CGTAGCG	25	3.8832448E-5	45.000004	1
GGTATAT	25	3.8832448E-5	45.000004	8
AAATTCG	30	2.159617E-6	45.000004	30
GCTAGTA	30	2.159617E-6	45.000004	34
GCGATGA	25	3.8832448E-5	45.000004	9
ACTAACG	30	2.159617E-6	45.000004	1
ACGTCCC	25	3.8832448E-5	45.000004	39
ATTGGGC	35	1.2075361E-7	45.000004	4
>>END_MODULE