FastQCFastQC Report
Sat 18 Jun 2016
SRR3554237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424393
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49821.1739119165490477No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9920.23374560843369235No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9670.22785484209211748No Hit
CTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGCT7570.17837240482288821No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4950.11663717356318318No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA4630.10909699264596731No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG253.8866317E-545.0000041
GCAAGCG253.8866317E-545.0000041
ATGATCG253.8866317E-545.0000041
GTCGAGC253.8866317E-545.00000411
GATCGGT253.8866317E-545.00000418
GTCGAAC253.8866317E-545.00000430
ACCGCAC253.8866317E-545.0000041
CAGCACG253.8866317E-545.0000041
CCGTCGA253.8866317E-545.0000049
ATCCGAT253.8866317E-545.00000422
GCCGACC253.8866317E-545.00000422
TAACCCG253.8866317E-545.0000041
TCAGCGT253.8866317E-545.00000423
CGTAGCG253.8866317E-545.0000041
CTACGAA750.045.00000410
CGTAAAG253.8866317E-545.0000041
TCCCCGT253.8866317E-545.00000424
CAACTCG253.8866317E-545.0000041
GATTACC253.8866317E-545.0000049
CGGAGGT253.8866317E-545.00000431
Produced by FastQC (version 0.11.5)