##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554237_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	424393
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35366747330894	34.0	31.0	34.0	30.0	34.0
2	32.52222114879369	34.0	31.0	34.0	31.0	34.0
3	32.606713588584164	34.0	31.0	34.0	31.0	34.0
4	36.12599877943322	37.0	35.0	37.0	35.0	37.0
5	35.97793790189753	37.0	35.0	37.0	35.0	37.0
6	35.967937736956074	37.0	35.0	37.0	35.0	37.0
7	36.05845289625418	37.0	35.0	37.0	35.0	37.0
8	36.18182439389905	37.0	37.0	37.0	35.0	37.0
9	37.85839304606815	39.0	38.0	39.0	35.0	39.0
10	37.353094890820536	39.0	37.0	39.0	35.0	39.0
11	37.29417073325903	39.0	37.0	39.0	34.0	39.0
12	37.334524839005354	39.0	37.0	39.0	34.0	39.0
13	37.342566913214874	39.0	37.0	39.0	34.0	39.0
14	38.61778351669325	40.0	38.0	41.0	34.0	41.0
15	38.60589123760288	40.0	38.0	41.0	34.0	41.0
16	38.59042679780298	40.0	38.0	41.0	34.0	41.0
17	38.54314986345204	40.0	38.0	41.0	34.0	41.0
18	38.44462797454246	40.0	38.0	41.0	34.0	41.0
19	38.41353886609817	40.0	38.0	41.0	34.0	41.0
20	38.348405840812646	40.0	38.0	41.0	34.0	41.0
21	38.30221987638816	40.0	38.0	41.0	34.0	41.0
22	38.256408564703	40.0	37.0	41.0	34.0	41.0
23	38.18581126455903	40.0	37.0	41.0	34.0	41.0
24	38.16123498738198	40.0	37.0	41.0	34.0	41.0
25	38.13448148296508	40.0	37.0	41.0	34.0	41.0
26	38.07655168676204	40.0	37.0	41.0	34.0	41.0
27	38.00554438928069	40.0	37.0	41.0	33.0	41.0
28	37.92690737123374	40.0	37.0	41.0	33.0	41.0
29	37.870900321164584	40.0	37.0	41.0	33.0	41.0
30	37.78458645642129	40.0	37.0	41.0	33.0	41.0
31	37.69931879178026	40.0	37.0	41.0	33.0	41.0
32	37.62968757731631	40.0	36.0	41.0	33.0	41.0
33	37.57588838647197	40.0	36.0	41.0	33.0	41.0
34	37.43939226141807	40.0	36.0	41.0	33.0	41.0
35	37.41016699144425	40.0	36.0	41.0	33.0	41.0
36	37.35355908320825	40.0	36.0	41.0	32.0	41.0
37	37.332762321715954	40.0	36.0	41.0	32.0	41.0
38	37.26935646912178	39.0	36.0	41.0	32.0	41.0
39	37.228382654756324	39.0	35.0	41.0	32.0	41.0
40	37.154783420084684	39.0	35.0	41.0	32.0	41.0
41	37.07923080729418	39.0	35.0	41.0	31.0	41.0
42	36.958316937367016	39.0	35.0	41.0	31.0	41.0
43	36.8839731098298	39.0	35.0	41.0	31.0	41.0
44	36.793264733395695	39.0	35.0	41.0	31.0	41.0
45	36.66798698376269	39.0	35.0	41.0	31.0	41.0
46	36.518125888033026	39.0	35.0	41.0	30.0	41.0
47	36.42123691955334	39.0	35.0	40.0	30.0	41.0
48	36.37370786040297	39.0	35.0	40.0	30.0	41.0
49	36.256234198019285	39.0	35.0	40.0	30.0	41.0
50	36.16542450040411	39.0	35.0	40.0	30.0	41.0
51	35.18242289575935	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	4.0
13	2.0
14	5.0
15	6.0
16	21.0
17	31.0
18	85.0
19	161.0
20	331.0
21	485.0
22	743.0
23	1046.0
24	1366.0
25	1777.0
26	2152.0
27	2763.0
28	3457.0
29	4367.0
30	5776.0
31	7658.0
32	10116.0
33	13847.0
34	21179.0
35	30314.0
36	35449.0
37	51065.0
38	89280.0
39	140842.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.848128974794587	33.844573308230814	21.57881963180354	23.728478085171055
2	22.034529315987776	31.282325580299393	21.114391613433774	25.568753490279057
3	19.516580150944996	33.36317988279731	22.47916435944985	24.641075606807842
4	19.906313252103594	32.91477474887663	20.903502178405393	26.27540982061438
5	18.333007377595766	35.38277021534286	17.922067517607502	28.362154889453876
6	20.052168626720988	38.392009293272984	19.57902227416569	21.97679980584034
7	87.5478624765253	5.710273260869053	3.487569304866009	3.2542949577396425
8	88.66522303619523	5.525303197743601	2.9906242562907495	2.818849509770425
9	80.56636183914438	11.167714830357712	5.2425464133480055	3.023376917149906
10	30.222458900123232	47.53447865539724	11.98229000007069	10.260772444408838
11	19.390753381888956	27.76553807437917	36.123592990459315	16.720115553272556
12	22.315165424500407	26.89558970105539	33.51304097852698	17.276203895917227
13	22.639864465248014	28.3951431809667	30.797633325714607	18.167359028070678
14	20.762123786207596	29.30137867495458	30.14729272160474	19.789204817233085
15	19.173973180519	31.84430468928564	30.509221405631102	18.472500724564263
16	20.871692040160887	31.529973397299198	29.35863692379469	18.23969763874522
17	20.550056197910898	30.907201579667902	30.046914063144303	18.495828159276897
18	21.965960795771842	29.942529683571596	29.766984846592663	18.3245246740639
19	20.162679403288934	32.28917536340138	28.539584771662113	19.008560461647576
20	21.686031579220206	32.36339901930522	29.035587297622723	16.914982103851855
21	21.722083069230642	31.41074428654573	29.13219586562455	17.734976778599084
22	21.124523731541284	29.843329178379474	30.15789610101957	18.874250989059668
23	19.639814982810744	31.54764569632393	29.66283609767362	19.14970322319171
24	17.72060330872564	31.27831985918712	31.97649348599058	19.02458334609666
25	19.07241636879025	32.60727674584642	29.08177090574067	19.23853597962266
26	20.94497317345008	31.617628000461835	28.021197333603524	19.41620149248456
27	19.687176744197004	31.817678425421718	28.442976203660287	20.052168626720988
28	18.572643752371036	32.77268946471785	30.265108048436236	18.389558734474885
29	19.881572033468977	30.917098067121746	29.684749748464277	19.516580150944996
30	20.526021871237273	30.805409137285487	29.94959860318149	18.718970388295755
31	21.014248585627	31.44891645243913	28.57728567624819	18.95954928568567
32	21.047001246486158	30.07330469635456	30.704323586864064	18.17537047029522
33	20.341287438765484	30.076603525505842	29.67084753989816	19.911261495830516
34	18.57782762675162	31.4102730252384	31.643547372364765	18.36835197564522
35	19.37213856024958	31.47954843741532	31.24957291944024	17.898740082894864
36	19.04579010492633	32.77928712302041	30.297153817334404	17.877768954718857
37	19.775538239320632	32.56557012014807	28.34141939193153	19.317472248599763
38	19.652303407454884	31.0549419995146	30.482359511113522	18.810395081916997
39	19.3108745902972	29.31009700914011	30.502388116674872	20.876640283887813
40	20.30075896633545	30.912385454048486	29.590261856345418	19.196593723270645
41	19.02387645413567	29.835553366808593	30.77242084577267	20.368149333283064
42	20.25198342102721	30.382687744614074	29.7323471405042	19.632981693854518
43	20.207449227484904	30.89070743391149	28.938978729620896	19.962864608982713
44	20.53591835869112	30.21939570162562	29.40010791883938	19.844578020843887
45	20.79157761791547	30.53137068707542	29.28299948396887	19.39405221104024
46	18.739941516471763	31.410508655892063	29.93946648507398	19.910083342562203
47	18.11552028426482	32.49016831097591	30.141401955263163	19.2529094494961
48	19.559700560565325	30.051155414910234	30.533020101651065	19.856123922873376
49	20.075260430779963	30.760168051782195	29.030403423242134	20.134168094195708
50	18.2175483573009	29.955725000176724	31.05329258493896	20.77343405758342
51	19.12284132867413	29.106512124375282	29.84921994472105	21.921426602229538
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5742.0
1	4494.5
2	3247.0
3	2033.0
4	819.0
5	757.0
6	695.0
7	845.0
8	995.0
9	1361.0
10	1727.0
11	1857.0
12	1987.0
13	2157.0
14	2327.0
15	2353.5
16	2380.0
17	2280.0
18	2180.0
19	2277.0
20	2374.0
21	2508.0
22	2642.0
23	3404.0
24	4166.0
25	4225.0
26	6013.0
27	7742.0
28	8987.0
29	10232.0
30	11732.5
31	13233.0
32	15860.0
33	18487.0
34	19975.0
35	21463.0
36	23704.0
37	25945.0
38	25767.0
39	25589.0
40	25740.5
41	25892.0
42	26298.5
43	26705.0
44	28860.0
45	31015.0
46	30193.5
47	29372.0
48	30017.5
49	30663.0
50	30289.5
51	29916.0
52	26573.0
53	23230.0
54	20457.5
55	17685.0
56	15738.0
57	13791.0
58	11885.0
59	9979.0
60	9620.5
61	9262.0
62	7959.5
63	6657.0
64	5438.0
65	4219.0
66	3482.0
67	2745.0
68	2210.0
69	1675.0
70	1342.5
71	1010.0
72	924.0
73	838.0
74	707.5
75	500.0
76	423.0
77	372.0
78	321.0
79	209.0
80	97.0
81	60.5
82	24.0
83	18.5
84	13.0
85	13.5
86	14.0
87	9.0
88	4.0
89	4.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	2.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	424393.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.034328274004686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.93927423087429	17.29012132658479
2	9.376335304262687	4.50707841419578
3	3.7879897193089227	2.7312536523727657
4	2.260107255702874	2.172806388720909
5	1.4746451596218677	1.7721052927012002
6	1.174911239407422	1.694292145244141
7	0.8871771911660467	1.4925895494785921
8	0.6873624568993801	1.321623594587688
9	0.6324134186601317	1.367966853806685
>10	6.350110088252395	32.906602322664085
>50	1.0290455303419233	17.23801516421125
>100	0.39663211218033356	13.657097194273204
>500	0.0029972199912871554	0.6521674516429782
>1k	9.990733304290518E-4	1.1962806495159488
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4982	1.1739119165490477	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	992	0.23374560843369235	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	967	0.22785484209211748	No Hit
CTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGCT	757	0.17837240482288821	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	495	0.11663717356318318	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	463	0.10909699264596731	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2045274073794808	0.0
2	0.0	0.0	0.0	0.2983084075373533	0.0
3	0.0	0.0	0.0	0.39962958861244174	0.0
4	0.0	0.0	0.0	0.89963783568532	0.0
5	0.0	0.0	0.0	0.9710339237452079	0.0
6	0.0	0.0	0.0	1.4312205903490396	0.0
7	0.0	0.0	0.0	1.7651092265895054	0.0
8	0.0	0.0	0.0	2.117377053815685	0.0
9	0.0	0.0	0.0	2.627517418996072	0.0
10	0.0	0.0	0.0	3.3709321313028253	0.0
11	0.0	0.0	0.0	4.034703682671486	0.0
12	0.0	0.0	0.0	4.339138487204077	0.0
13	0.0	0.0	0.0	4.473919221099311	0.0
14	0.0	0.0	0.0	4.617653919833739	0.0
15	0.0	0.0	0.0	4.7404174903921605	0.0
16	0.0	0.0	0.0	4.980525126474753	0.0
17	0.0	0.0	0.0	5.31606317729086	0.0
18	0.0	0.0	0.0	5.7237042081278435	0.0
19	0.0	0.0	0.0	5.912679992365566	0.0
20	0.0	0.0	0.0	6.105661497715561	0.0
21	0.0	0.0	0.0	6.32503363627581	0.0
22	0.0	0.0	0.0	6.589882490993018	0.0
23	0.0	0.0	0.0	6.8441279662953916	0.0
24	0.0	0.0	0.0	7.046299067138242	0.0
25	0.0	0.0	0.0	7.207941695551058	0.0
26	0.0	0.0	0.0	7.377595766188415	0.0
27	0.0	0.0	0.0	7.570106010231083	0.0
28	0.0	0.0	0.0	7.746357739171004	0.0
29	2.3563065366299634E-4	0.0	0.0	7.9490001013211815	0.0
30	2.3563065366299634E-4	0.0	0.0	8.217619046496997	0.0
31	4.7126130732599267E-4	0.0	0.0	8.424502760413107	0.0
32	4.7126130732599267E-4	0.0	0.0	8.619840572299731	0.0
33	4.7126130732599267E-4	0.0	0.0	8.815649645493682	0.0
34	4.7126130732599267E-4	0.0	0.0	9.003682907116753	0.0
35	4.7126130732599267E-4	0.0	0.0	9.223997568291654	0.0
36	4.7126130732599267E-4	0.0	0.0	9.42805371436381	0.0
37	4.7126130732599267E-4	0.0	0.0	9.638471888084865	0.0
38	4.7126130732599267E-4	0.0	0.0	9.865855468869656	0.0
39	4.7126130732599267E-4	0.0	0.0	10.114917069791444	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	25	3.8866317E-5	45.000004	1
GCAAGCG	25	3.8866317E-5	45.000004	1
ATGATCG	25	3.8866317E-5	45.000004	1
GTCGAGC	25	3.8866317E-5	45.000004	11
GATCGGT	25	3.8866317E-5	45.000004	18
GTCGAAC	25	3.8866317E-5	45.000004	30
ACCGCAC	25	3.8866317E-5	45.000004	1
CAGCACG	25	3.8866317E-5	45.000004	1
CCGTCGA	25	3.8866317E-5	45.000004	9
ATCCGAT	25	3.8866317E-5	45.000004	22
GCCGACC	25	3.8866317E-5	45.000004	22
TAACCCG	25	3.8866317E-5	45.000004	1
TCAGCGT	25	3.8866317E-5	45.000004	23
CGTAGCG	25	3.8866317E-5	45.000004	1
CTACGAA	75	0.0	45.000004	10
CGTAAAG	25	3.8866317E-5	45.000004	1
TCCCCGT	25	3.8866317E-5	45.000004	24
CAACTCG	25	3.8866317E-5	45.000004	1
GATTACC	25	3.8866317E-5	45.000004	9
CGGAGGT	25	3.8866317E-5	45.000004	31
>>END_MODULE