FastQCFastQC Report
Sat 18 Jun 2016
SRR3554235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433923
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52591.2119661783311786No Hit
CTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGCT10560.24336114932833708No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9320.21478465073296416No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8050.18551678523609028No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4990.1149973612829926No Hit
AAGCTGGGGCTTGTTGTGTTACCTGTAAAATAAGTATTAGTTTATAAACAG4480.10324412395747633No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACC253.8867773E-545.00000445
AGTATAC253.8867773E-545.00000422
TAGCACG253.8867773E-545.0000041
CACTAGA253.8867773E-545.00000437
CGCTAAG351.2095916E-745.0000041
TATTACG253.8867773E-545.0000041
CCCTTAC253.8867773E-545.00000445
TTACGTC253.8867773E-545.00000443
ATTGCGG253.8867773E-545.0000042
TGTACCG253.8867773E-545.0000041
TAATCCG253.8867773E-545.0000041
CCGGGTC406.7975634E-945.05
GTACCGA207.028428E-445.013
ATCTCCG207.028428E-445.026
ACTGCCG207.028428E-445.01
GTTTCGC207.028428E-445.09
CATGCGG207.028428E-445.02
GGCCGTA207.028428E-445.09
CGACCCG207.028428E-445.032
TGAAACG207.028428E-445.01