##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554235_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	433923
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22777082569949	33.0	31.0	34.0	30.0	34.0
2	32.408279810012374	34.0	31.0	34.0	30.0	34.0
3	32.50613588125082	34.0	31.0	34.0	30.0	34.0
4	36.02842670243338	37.0	35.0	37.0	35.0	37.0
5	35.85845875881205	37.0	35.0	37.0	35.0	37.0
6	35.83384148800594	37.0	35.0	37.0	35.0	37.0
7	35.86937083307407	37.0	35.0	37.0	35.0	37.0
8	35.9885302231041	37.0	35.0	37.0	35.0	37.0
9	37.62369360462571	39.0	37.0	39.0	35.0	39.0
10	37.22152086890992	39.0	37.0	39.0	34.0	39.0
11	37.19710409450525	39.0	37.0	39.0	34.0	39.0
12	37.16187895087377	39.0	37.0	39.0	34.0	39.0
13	37.136989742419736	39.0	37.0	39.0	33.0	39.0
14	38.36032429716793	40.0	38.0	41.0	34.0	41.0
15	38.3816806207553	40.0	38.0	41.0	34.0	41.0
16	38.342961769714904	40.0	38.0	41.0	34.0	41.0
17	38.29665401465237	40.0	38.0	41.0	34.0	41.0
18	38.215434535620375	40.0	37.0	41.0	33.0	41.0
19	38.16992646160725	40.0	37.0	41.0	34.0	41.0
20	38.09959140216121	40.0	37.0	41.0	33.0	41.0
21	38.03282379592693	40.0	37.0	41.0	33.0	41.0
22	37.96632121367155	40.0	37.0	41.0	33.0	41.0
23	37.83623131292879	40.0	37.0	41.0	33.0	41.0
24	37.85039050707153	40.0	37.0	41.0	33.0	41.0
25	37.78086895601293	40.0	36.0	41.0	33.0	41.0
26	37.72035361112455	40.0	36.0	41.0	33.0	41.0
27	37.66474005756782	40.0	36.0	41.0	33.0	41.0
28	37.52634223122536	40.0	36.0	41.0	33.0	41.0
29	37.4688158959078	40.0	36.0	41.0	32.0	41.0
30	37.40482758461755	40.0	36.0	41.0	32.0	41.0
31	37.27554427859321	39.0	36.0	41.0	32.0	41.0
32	37.21978553798716	39.0	36.0	41.0	32.0	41.0
33	37.14727267280139	39.0	35.0	41.0	31.0	41.0
34	37.040583237118106	39.0	35.0	41.0	31.0	41.0
35	36.93871262873828	39.0	35.0	41.0	31.0	41.0
36	36.842548101852174	39.0	35.0	41.0	31.0	41.0
37	36.78969079767609	39.0	35.0	41.0	31.0	41.0
38	36.69749932591727	39.0	35.0	41.0	31.0	41.0
39	36.64846758526282	39.0	35.0	41.0	30.0	41.0
40	36.536860226353525	39.0	35.0	41.0	30.0	41.0
41	36.487487411361	39.0	35.0	41.0	30.0	41.0
42	36.41883928715463	39.0	35.0	40.0	30.0	41.0
43	36.37760155603644	39.0	35.0	40.0	30.0	41.0
44	36.271721019627904	39.0	35.0	40.0	30.0	41.0
45	36.077218769228644	38.0	35.0	40.0	29.0	41.0
46	35.920140209207624	38.0	35.0	40.0	29.0	41.0
47	35.83052753599141	38.0	35.0	40.0	29.0	41.0
48	35.78284165623855	38.0	35.0	40.0	28.0	41.0
49	35.646091587678	38.0	34.0	40.0	28.0	41.0
50	35.54116744214988	38.0	34.0	40.0	28.0	41.0
51	34.56900648271698	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	1.0
11	1.0
12	2.0
13	3.0
14	7.0
15	21.0
16	43.0
17	74.0
18	130.0
19	232.0
20	460.0
21	662.0
22	1016.0
23	1429.0
24	1847.0
25	2333.0
26	2772.0
27	3426.0
28	4417.0
29	5739.0
30	7276.0
31	9393.0
32	12074.0
33	16286.0
34	24958.0
35	34378.0
36	37897.0
37	54029.0
38	87987.0
39	124970.0
40	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.10213102324606	32.05246092048589	20.340936064693505	26.50447199157454
2	24.13262260815859	31.386213683072807	20.17546891960094	24.305694789167664
3	21.314611117640688	32.02181032118602	20.187683068194126	26.475895492979166
4	20.507325032321404	32.337304083904286	19.736911848415502	27.418459035358804
5	18.30370826160402	33.83226978058319	18.781212334907345	29.082809622905447
6	20.95579169576169	37.96364792831908	20.313050932999634	20.767509442919597
7	83.00551019420497	7.420901865077445	5.295640009863501	4.277947930854092
8	83.85312601544513	7.036501867842912	4.903865432346292	4.206506684365659
9	74.93080569594144	10.992733733865224	8.634250777211625	5.4422097929817035
10	32.23475132684831	35.8353901498653	15.798194610564547	16.13166391272184
11	24.550438672299002	30.72042735692738	27.471002919872884	17.258131050900737
12	23.944340355316495	27.39840939521528	30.384192587164083	18.27305766230414
13	23.494260502439374	29.135814418687183	28.156147519260326	19.21377755961311
14	19.585041585719125	29.424805783514586	30.098888512478023	20.891264118288266
15	19.04992360395738	29.699508899044304	31.08961728232889	20.160950214669423
16	20.64467659008626	30.35492472166721	28.56244080170906	20.437957886537472
17	19.940404173090617	29.766571488489895	29.44278132295361	20.850243015465878
18	22.301652597350223	28.825160224279422	29.06275998276192	19.810427195608437
19	21.082311838736363	30.07999115050366	28.477402672824443	20.36029433793553
20	23.491264579199537	28.891300991189684	28.705784205953588	18.91165022365719
21	22.477720701599132	29.808975325115284	27.97247437909491	19.74082959419067
22	22.461358351596942	29.25634271518219	27.36061467126656	20.92168426195431
23	20.427356927381123	30.499881315348574	28.22528420941042	20.847477547859874
24	19.552086430080912	29.961536954713164	30.393180356883594	20.09319625832233
25	20.514930068237913	32.032411280342366	27.071162395171495	20.381496256248226
26	22.496618063573493	30.438580116748824	25.90597871050855	21.158823109169138
27	20.450632946398322	29.640742712416717	27.058948246578314	22.849676094606647
28	19.132196265235997	30.9773853886519	29.068060462340096	20.822357883772007
29	19.24604134834982	30.125160454735056	28.1114391262966	22.51735907061852
30	20.36398162807687	30.86307939427041	28.94684079894359	19.826098178709124
31	20.682932225302647	32.120445332466815	26.524291176084237	20.672331266146298
32	21.39365740004563	29.1687695743254	27.462476061421036	21.975096964207935
33	20.32549553722665	30.06985110261498	27.144908197998262	22.459745162160107
34	20.31235956609813	29.08557509051145	28.53524703691669	22.066818306473728
35	20.27617803158625	30.20743311601367	28.927482525701564	20.588906326698517
36	19.325087630754766	33.28562901712977	27.815534092454193	19.573749259661277
37	20.00539266183171	32.072280104995585	27.25183961209708	20.67048762107563
38	18.987009215920796	32.403444850814545	29.819576284271633	18.789969648993026
39	19.48318019556465	31.18663910417286	27.872456634011105	21.457724066251384
40	20.923297451391146	31.78974149791553	27.450722824095518	19.836238226597807
41	18.70654470954524	29.54694726944642	30.379583474487408	21.366924546520927
42	20.144587864667233	30.49020217872756	28.187028574194038	21.178181382411164
43	19.491015687114995	29.183057823623088	29.235371252503324	22.090555236758597
44	20.909239657727294	28.93232209401207	28.102912267844754	22.05552598041588
45	19.734607292077165	30.733332872422064	28.939005307393245	20.593054528107523
46	18.040988839033652	30.916775556953652	29.910375804002094	21.131859800010602
47	18.382524088375128	31.40004102110282	28.89660147076786	21.320833419754194
48	19.32347444131793	28.597930969319442	30.26619930264125	21.812395286721376
49	19.725158611089984	29.803444389903277	29.525284439866063	20.94611255914068
50	19.376709692733503	30.39110625617909	28.416101474224693	21.816082576862716
51	20.302680429477117	27.486443447339738	30.311829518140314	21.89904660504283
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6093.0
1	4535.5
2	2978.0
3	1928.5
4	879.0
5	767.5
6	656.0
7	768.5
8	881.0
9	1056.0
10	1231.0
11	1355.0
12	1479.0
13	1543.0
14	1607.0
15	1636.5
16	1666.0
17	1813.5
18	1961.0
19	1906.0
20	1851.0
21	2227.5
22	2604.0
23	2946.0
24	3288.0
25	3927.5
26	4995.5
27	5424.0
28	6950.0
29	8476.0
30	10666.5
31	12857.0
32	14402.5
33	15948.0
34	17689.0
35	19430.0
36	21503.5
37	23577.0
38	23446.5
39	23316.0
40	24564.0
41	25812.0
42	25874.0
43	25936.0
44	27084.5
45	28233.0
46	29187.0
47	30141.0
48	30843.5
49	31546.0
50	30813.5
51	30081.0
52	27475.5
53	24870.0
54	23401.0
55	21932.0
56	18766.5
57	15601.0
58	14243.0
59	12885.0
60	12634.5
61	12384.0
62	10994.0
63	9604.0
64	8330.0
65	7056.0
66	6243.5
67	5431.0
68	4508.0
69	3585.0
70	3146.5
71	2708.0
72	2353.5
73	1999.0
74	1728.0
75	1255.5
76	1054.0
77	795.0
78	536.0
79	336.0
80	136.0
81	103.0
82	70.0
83	50.5
84	31.0
85	23.5
86	16.0
87	18.5
88	21.0
89	16.5
90	12.0
91	11.0
92	10.0
93	7.0
94	4.0
95	3.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	433923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.699649559732695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.63159091186485	17.45042901136796
2	9.693679929055062	4.59473634525639
3	3.6257308215712594	2.5778564960748165
4	1.836350033085752	1.7408340901254342
5	1.2169397538735909	1.4420522851055728
6	0.870497960950195	1.23782979701889
7	0.6788258173567412	1.126155378841771
8	0.5410638099224311	1.0258418147692352
9	0.4781725948587411	1.0199270634498183
>10	5.922751514139337	31.33903900888088
>50	0.9942795394218656	16.874575973587216
>100	0.5061242233804075	17.66572328596963
>500	0.0019965452598832644	0.4109518186627549
>1k	9.982726299416322E-4	0.24983599338392007
>5k	9.982726299416322E-4	1.2442116375057157
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5259	1.2119661783311786	No Hit
CTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGCT	1056	0.24336114932833708	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	932	0.21478465073296416	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	805	0.18551678523609028	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	499	0.1149973612829926	No Hit
AAGCTGGGGCTTGTTGTGTTACCTGTAAAATAAGTATTAGTTTATAAACAG	448	0.10324412395747633	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.28530407468606184	0.0
2	0.0	0.0	0.0	0.3940791338555458	0.0
3	0.0	0.0	0.0	0.5210601880978883	0.0
4	0.0	0.0	0.0	1.1465167783224213	0.0
5	0.0	0.0	0.0	1.2283285283333678	0.0
6	0.0	0.0	0.0	1.768055622771782	0.0
7	0.0	0.0	0.0	2.1842584974753585	0.0
8	0.0	0.0	0.0	2.594008614431593	0.0
9	0.0	0.0	0.0	3.2037942215554374	0.0
10	0.0	0.0	0.0	4.093352968153336	0.0
11	0.0	0.0	0.0	4.864457518960737	0.0
12	0.0	0.0	0.0	5.274899002818472	0.0
13	0.0	0.0	0.0	5.438522502840366	0.0
14	0.0	0.0	0.0	5.621504276104286	0.0
15	0.0	0.0	0.0	5.798494202888531	0.0
16	0.0	0.0	0.0	6.135420339553331	0.0
17	0.0	0.0	0.0	6.526042638901372	0.0
18	0.0	0.0	0.0	7.0835148171449775	0.0
19	0.0	0.0	0.0	7.2872375974539265	0.0
20	0.0	0.0	0.0	7.5547965883347965	0.0
21	0.0	0.0	0.0	7.834108816541184	0.0
22	0.0	0.0	0.0	8.112960133479904	0.0
23	0.0	0.0	0.0	8.423383872253833	0.0
24	0.0	0.0	0.0	8.652456772284484	0.0
25	0.0	0.0	0.0	8.86101912090394	0.0
26	0.0	0.0	0.0	9.060363244170048	0.0
27	0.0	0.0	0.0	9.300958925892381	0.0
28	0.0	0.0	0.0	9.519430866766685	0.0
29	0.0	0.0	0.0	9.748042855529668	0.0
30	0.0	0.0	0.0	10.08128170205313	0.0
31	0.0	0.0	0.0	10.31035460208378	0.0
32	0.0	0.0	0.0	10.528365631690415	0.0
33	0.0	0.0	0.0	10.779331816935263	0.0
34	0.0	0.0	0.0	11.034215747955283	0.0
35	0.0	0.0	0.0	11.313067064894001	0.0
36	0.0	0.0	0.0	11.564263705772683	0.0
37	0.0	0.0	0.0	11.795410706507838	0.0
38	0.0	0.0	0.0	12.04614643611885	0.0
39	0.0	0.0	0.0	12.330298232635744	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACC	25	3.8867773E-5	45.000004	45
AGTATAC	25	3.8867773E-5	45.000004	22
TAGCACG	25	3.8867773E-5	45.000004	1
CACTAGA	25	3.8867773E-5	45.000004	37
CGCTAAG	35	1.2095916E-7	45.000004	1
TATTACG	25	3.8867773E-5	45.000004	1
CCCTTAC	25	3.8867773E-5	45.000004	45
TTACGTC	25	3.8867773E-5	45.000004	43
ATTGCGG	25	3.8867773E-5	45.000004	2
TGTACCG	25	3.8867773E-5	45.000004	1
TAATCCG	25	3.8867773E-5	45.000004	1
CCGGGTC	40	6.7975634E-9	45.0	5
GTACCGA	20	7.028428E-4	45.0	13
ATCTCCG	20	7.028428E-4	45.0	26
ACTGCCG	20	7.028428E-4	45.0	1
GTTTCGC	20	7.028428E-4	45.0	9
CATGCGG	20	7.028428E-4	45.0	2
GGCCGTA	20	7.028428E-4	45.0	9
CGACCCG	20	7.028428E-4	45.0	32
TGAAACG	20	7.028428E-4	45.0	1
>>END_MODULE