Basic Statistics
Measure | Value |
---|---|
Filename | SRR3554232_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 346588 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 41 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 8467 | 2.4429582097475966 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1584 | 0.4570267868477847 | No Hit |
CTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT | 1235 | 0.35633085969508466 | TruSeq Adapter, Index 14 (95% over 22bp) |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1185 | 0.3419045091001419 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 838 | 0.24178563597123962 | No Hit |
AACACGGGATCAGAGAATAGAAAGCAGTAAAAGAGAATGATTTTGGTTTGC | 454 | 0.1309912634020797 | No Hit |
AAGGTAGGGGTTGTTTTGGTTTTCAAAAGAAAAGGAAAAAAATCCACTTTT | 410 | 0.11829607487853014 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGATCGG | 30 | 2.1610977E-6 | 45.000004 | 2 |
ACGCGAC | 20 | 7.0264586E-4 | 45.000004 | 18 |
CGGGTTG | 40 | 6.7921064E-9 | 45.000004 | 6 |
ACAACCG | 30 | 2.1610977E-6 | 45.000004 | 1 |
TTTAGCG | 30 | 2.1610977E-6 | 45.000004 | 1 |
CTTCGCG | 25 | 3.8851475E-5 | 45.000004 | 1 |
CGTGGTC | 20 | 7.0264586E-4 | 45.000004 | 18 |
AGCGTAT | 20 | 7.0264586E-4 | 45.000004 | 12 |
AGCCGTA | 25 | 3.8851475E-5 | 45.000004 | 25 |
TCGTTGG | 30 | 2.1610977E-6 | 45.000004 | 2 |
CGAACGC | 20 | 7.0264586E-4 | 45.000004 | 33 |
CGAACCG | 30 | 2.1610977E-6 | 45.000004 | 1 |
CTCGTTG | 20 | 7.0264586E-4 | 45.000004 | 1 |
CGTATTC | 20 | 7.0264586E-4 | 45.000004 | 14 |
CATATCG | 30 | 2.1610977E-6 | 45.000004 | 1 |
TCACCCG | 30 | 2.1610977E-6 | 45.000004 | 1 |
CGAGTTT | 20 | 7.0264586E-4 | 45.000004 | 15 |
ATAGGGC | 50 | 2.1827873E-11 | 45.000004 | 4 |
CGAAACT | 20 | 7.0264586E-4 | 45.000004 | 12 |
CGAAAAC | 20 | 7.0264586E-4 | 45.000004 | 29 |