FastQCFastQC Report
Sat 18 Jun 2016
SRR3554232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346588
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84672.4429582097475966No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15840.4570267868477847No Hit
CTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT12350.35633085969508466TruSeq Adapter, Index 14 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11850.3419045091001419No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8380.24178563597123962No Hit
AACACGGGATCAGAGAATAGAAAGCAGTAAAAGAGAATGATTTTGGTTTGC4540.1309912634020797No Hit
AAGGTAGGGGTTGTTTTGGTTTTCAAAAGAAAAGGAAAAAAATCCACTTTT4100.11829607487853014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGG302.1610977E-645.0000042
ACGCGAC207.0264586E-445.00000418
CGGGTTG406.7921064E-945.0000046
ACAACCG302.1610977E-645.0000041
TTTAGCG302.1610977E-645.0000041
CTTCGCG253.8851475E-545.0000041
CGTGGTC207.0264586E-445.00000418
AGCGTAT207.0264586E-445.00000412
AGCCGTA253.8851475E-545.00000425
TCGTTGG302.1610977E-645.0000042
CGAACGC207.0264586E-445.00000433
CGAACCG302.1610977E-645.0000041
CTCGTTG207.0264586E-445.0000041
CGTATTC207.0264586E-445.00000414
CATATCG302.1610977E-645.0000041
TCACCCG302.1610977E-645.0000041
CGAGTTT207.0264586E-445.00000415
ATAGGGC502.1827873E-1145.0000044
CGAAACT207.0264586E-445.00000412
CGAAAAC207.0264586E-445.00000429