##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554232_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346588
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.073790783293134	33.0	31.0	34.0	30.0	34.0
2	32.27108555402957	34.0	31.0	34.0	30.0	34.0
3	32.3760863041998	34.0	31.0	34.0	30.0	34.0
4	35.94217053100511	37.0	35.0	37.0	35.0	37.0
5	35.757308389211396	37.0	35.0	37.0	35.0	37.0
6	35.735942963980285	37.0	35.0	37.0	33.0	37.0
7	35.84648054750886	37.0	35.0	37.0	35.0	37.0
8	35.99357450344501	37.0	35.0	37.0	35.0	37.0
9	37.622872113287244	39.0	37.0	39.0	35.0	39.0
10	37.06712869458839	39.0	37.0	39.0	33.0	39.0
11	37.04772525303819	39.0	37.0	39.0	33.0	39.0
12	37.14308343047076	39.0	37.0	39.0	34.0	39.0
13	37.137102265514095	39.0	37.0	39.0	33.0	39.0
14	38.3567434533221	40.0	38.0	41.0	34.0	41.0
15	38.33989059055709	40.0	38.0	41.0	34.0	41.0
16	38.34079945064457	40.0	38.0	41.0	34.0	41.0
17	38.26260574514986	40.0	38.0	41.0	33.0	41.0
18	38.07866688979422	40.0	37.0	41.0	33.0	41.0
19	38.01503225731993	40.0	37.0	41.0	33.0	41.0
20	37.92953016261382	40.0	37.0	41.0	33.0	41.0
21	37.88330236476739	40.0	37.0	41.0	33.0	41.0
22	37.842403660830726	40.0	37.0	41.0	33.0	41.0
23	37.713954320403474	39.0	36.0	41.0	33.0	41.0
24	37.67959940909668	39.0	36.0	41.0	33.0	41.0
25	37.6320732396967	39.0	36.0	41.0	33.0	41.0
26	37.50250152919316	39.0	36.0	41.0	33.0	41.0
27	37.45286911260632	39.0	36.0	41.0	32.0	41.0
28	37.39851639410482	39.0	36.0	41.0	32.0	41.0
29	37.303645827322356	39.0	36.0	41.0	32.0	41.0
30	37.22785266656664	39.0	36.0	41.0	32.0	41.0
31	37.19485094694565	39.0	36.0	41.0	32.0	41.0
32	37.14831153992637	39.0	35.0	41.0	32.0	41.0
33	37.0568888709361	39.0	35.0	41.0	31.0	41.0
34	36.995213336872595	39.0	35.0	41.0	31.0	41.0
35	36.93099587983427	39.0	35.0	41.0	31.0	41.0
36	36.83056539753252	39.0	35.0	41.0	31.0	41.0
37	36.763707918335314	39.0	35.0	41.0	31.0	41.0
38	36.684723648828005	39.0	35.0	40.0	31.0	41.0
39	36.622098860895356	39.0	35.0	40.0	30.0	41.0
40	36.58076159590061	39.0	35.0	40.0	30.0	41.0
41	36.497054139208515	39.0	35.0	40.0	30.0	41.0
42	36.40394647246875	39.0	35.0	40.0	30.0	41.0
43	36.34900804413309	39.0	35.0	40.0	30.0	41.0
44	36.26308469998961	39.0	35.0	40.0	30.0	41.0
45	36.099083061156186	38.0	35.0	40.0	30.0	41.0
46	35.991797177051716	38.0	35.0	40.0	29.0	41.0
47	35.81359423869262	38.0	35.0	40.0	28.0	41.0
48	35.75727088069985	38.0	35.0	40.0	28.0	41.0
49	35.61593015338096	38.0	35.0	40.0	28.0	41.0
50	35.48068023128325	38.0	34.0	40.0	27.0	41.0
51	34.4647477696862	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	3.0
13	3.0
14	4.0
15	10.0
16	30.0
17	49.0
18	111.0
19	208.0
20	375.0
21	611.0
22	854.0
23	1213.0
24	1615.0
25	1902.0
26	2248.0
27	2788.0
28	3539.0
29	4599.0
30	5760.0
31	7511.0
32	10107.0
33	13319.0
34	19059.0
35	29696.0
36	31951.0
37	44029.0
38	71306.0
39	93657.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.79064480016619	35.41640218357243	21.546908721594516	23.246044294666866
2	21.30945099080176	34.534663635209526	20.772213694646094	23.383671679342623
3	19.54914769120685	34.076194213302244	21.97392869920482	24.40072939628608
4	19.77881519267834	35.106812699804955	20.006174478054636	25.10819762946207
5	18.02774475746419	37.492354034184686	17.629866008055675	26.85003520029545
6	20.135146052373422	39.20187657968539	19.270430597712558	21.39254677022863
7	84.57909679504195	8.09895322400083	4.09362124482094	3.2283287361362776
8	85.88929795607466	8.102127021131718	2.9452837374635013	3.063291285330133
9	77.0670075132434	13.342354611238704	6.478296998164969	3.112340877352938
10	26.582570660265215	51.65152861610905	11.572529920251135	10.193370803374611
11	16.037773956397796	30.883065772617634	37.77713019492885	15.30203007605572
12	19.970685655591076	27.822659757406488	34.44579731554468	17.760857271457752
13	21.449386591572704	29.990940251826377	31.038581832031113	17.521091324569806
14	17.057428416448346	31.737971308873934	31.510323496485743	19.694276778191973
15	16.381121100557433	34.99111336803352	31.459831269403445	17.16793426200561
16	19.1841610211548	33.45643819174351	30.054704721456023	17.30469606564567
17	19.75342481563124	31.77836509053978	30.165499094025183	18.3027109998038
18	18.79840040624603	32.44890186619271	30.34842521956906	18.404272507992196
19	19.074232229621337	33.54530451140836	30.378143501794636	17.002319757175666
20	19.064710838228674	33.09953027802463	30.070285180098562	17.765473703648134
21	19.658787955728414	33.29803686221104	29.753771048045518	17.289404134015026
22	18.639133495677864	32.89871547774303	29.414463282052466	19.04768774452664
23	18.06669590407054	33.803824714069734	29.62970443292901	18.49977494893072
24	16.579916211755744	33.2322527034981	30.458354011102518	19.729477073643633
25	18.30040278370861	35.316860364467324	27.522303138019783	18.860433713804287
26	18.249333502602514	34.85060071323877	29.09910325804702	17.800962526111693
27	17.580816416032867	33.493658176278466	29.252888155389105	19.67263725229956
28	17.706902720232666	33.69476150357196	31.293639710549705	17.30469606564567
29	18.497466732835527	32.79859660461412	29.529874086812008	19.17406257573834
30	19.381224970281718	33.64802012764435	29.21451406280656	17.756240839267374
31	18.728865396378406	33.51298948607568	28.487137465809546	19.271007651736355
32	19.973570925710067	32.094013641557126	28.545131395201217	19.387284037531593
33	18.730019504426004	32.0501575357485	30.322746315510056	18.89707664431544
34	18.173162371461217	31.85251653259778	30.83834408577331	19.135977010167693
35	19.127609726822627	31.228144078848665	30.81093401964292	18.833312174685794
36	18.15902454787817	32.260205200410866	31.490703659676615	18.090066592034347
37	18.896499590291643	32.68635959698547	29.419656768266645	18.997484044456243
38	18.689625722760166	31.841552506145625	31.54234999480651	17.9264717762877
39	18.797823352222235	31.37760107101227	29.925156093113436	19.89941948365206
40	20.157074105277736	32.013803132249244	28.24564035685021	19.583482405622814
41	17.18005239650536	30.88652809676042	30.774579616143665	21.15883989059056
42	18.950742668528626	31.740856578992926	29.520352695419344	19.7880480570591
43	17.870497535979318	30.814684870797603	30.829111221392548	20.48570637183053
44	19.91182614516371	31.312682493335025	28.68045056378178	20.09504079771948
45	19.569633109051672	31.335187600263136	29.949392362112942	19.145786928572253
46	18.67577642618902	32.18807344743615	30.11818066407377	19.01796946230106
47	18.40802335914688	31.347017207750987	30.69667732293097	19.548282110171154
48	19.63022378155043	29.662596512285482	30.45604579500733	20.251133911156764
49	18.470633720728934	31.61996376100731	29.35964314979168	20.549759368472074
50	18.486214179371473	30.650513001027157	29.23182568352049	21.63144713608088
51	18.321465255577227	29.272796519210125	31.126582570660265	21.27915565455238
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9651.0
1	6991.5
2	4332.0
3	2691.0
4	1050.0
5	868.5
6	687.0
7	781.5
8	876.0
9	1046.5
10	1217.0
11	1412.0
12	1607.0
13	1868.5
14	2130.0
15	2045.0
16	1960.0
17	2056.5
18	2153.0
19	1846.0
20	1539.0
21	2216.0
22	2893.0
23	3172.5
24	3452.0
25	3611.5
26	4855.0
27	5939.0
28	6916.0
29	7893.0
30	10305.5
31	12718.0
32	13798.0
33	14878.0
34	15972.5
35	17067.0
36	17914.5
37	18762.0
38	20267.0
39	21772.0
40	21928.0
41	22084.0
42	22478.0
43	22872.0
44	23868.5
45	24865.0
46	26272.0
47	27679.0
48	28687.0
49	29695.0
50	27046.0
51	24397.0
52	21531.5
53	18666.0
54	15211.5
55	11757.0
56	10023.5
57	8290.0
58	7265.0
59	6240.0
60	5332.0
61	4424.0
62	3946.0
63	3468.0
64	2803.0
65	2138.0
66	1706.5
67	1275.0
68	1080.5
69	886.0
70	737.5
71	589.0
72	466.5
73	344.0
74	317.5
75	225.5
76	160.0
77	109.0
78	58.0
79	45.0
80	32.0
81	26.5
82	21.0
83	12.0
84	3.0
85	4.0
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	346588.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.1966542562067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.64595199371433	21.794120519858808
2	9.809366136138697	5.728013430987676
3	3.5975583507631783	3.1511000200128514
4	2.00252754734666	2.338684177536401
5	1.3096837197641904	1.9119191375468887
6	0.940772551967455	1.6480446560113822
7	0.7398211254926811	1.5120211128713261
8	0.578860042401148	1.3520621216575575
9	0.5098767212693983	1.3398024909771806
>10	4.907813381778991	30.272530792812674
>50	0.6778361118040598	13.679120726483221
>100	0.27493352617421307	11.387737381351812
>500	9.997582769971384E-4	0.24460882079237223
>1k	0.002999274830991415	1.1687514540007857
>5k	9.997582769971384E-4	2.471483157099064
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8467	2.4429582097475966	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1584	0.4570267868477847	No Hit
CTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT	1235	0.35633085969508466	TruSeq Adapter, Index 14 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1185	0.3419045091001419	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	838	0.24178563597123962	No Hit
AACACGGGATCAGAGAATAGAAAGCAGTAAAAGAGAATGATTTTGGTTTGC	454	0.1309912634020797	No Hit
AAGGTAGGGGTTGTTTTGGTTTTCAAAAGAAAAGGAAAAAAATCCACTTTT	410	0.11829607487853014	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4192297482890348	0.0
2	0.0	0.0	0.0	0.6151395893683567	0.0
3	0.0	0.0	0.0	0.8188396597689476	0.0
4	0.0	0.0	0.0	1.825798931295948	0.0
5	0.0	0.0	0.0	1.9717935993167681	0.0
6	0.0	0.0	0.0	2.8673814442508108	0.0
7	0.0	0.0	0.0	3.4605929807148543	0.0
8	0.0	0.0	0.0	4.008794303322677	0.0
9	0.0	0.0	0.0	4.84148325966277	0.0
10	0.0	0.0	0.0	6.208235715027641	0.0
11	0.0	0.0	0.0	7.363786397682551	0.0
12	0.0	0.0	0.0	7.954401191039505	0.0
13	0.0	0.0	0.0	8.198495043105936	0.0
14	0.0	0.0	0.0	8.45672671875541	0.0
15	0.0	0.0	0.0	8.687259801262595	0.0
16	0.0	0.0	0.0	9.152365344443547	0.0
17	0.0	0.0	0.0	9.801839648227867	0.0
18	0.0	0.0	0.0	10.668574791972025	0.0
19	0.0	0.0	0.0	11.0523157177975	0.0
20	0.0	0.0	0.0	11.468371668955648	0.0
21	0.0	0.0	0.0	11.901450713815828	0.0
22	0.0	0.0	0.0	12.408681200734012	0.0
23	0.0	0.0	0.0	12.876672014033954	0.0
24	0.0	0.0	0.0	13.216268307038906	0.0
25	0.0	0.0	0.0	13.50970027814004	0.0
26	0.0	0.0	0.0	13.816981545812318	0.0
27	0.0	0.0	0.0	14.144459704317518	0.0
28	0.0	0.0	0.0	14.49415444273893	0.0
29	0.0	0.0	0.0	14.853947626576801	0.0
30	0.0	0.0	0.0	15.301741549043822	0.0
31	0.0	0.0	0.0	15.6344131937632	0.0
32	0.0	0.0	0.0	15.966796311470679	0.0
33	0.0	0.0	0.0	16.29340888894018	0.0
34	0.0	0.0	0.0	16.62608053365956	0.0
35	0.0	0.0	0.0	16.99654921693769	0.0
36	0.0	0.0	0.0	17.320276524288204	0.0
37	0.0	0.0	0.0	17.68324350525696	0.0
38	0.0	0.0	0.0	18.03582351379736	0.0
39	0.0	0.0	0.0	18.38378709014738	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	30	2.1610977E-6	45.000004	2
ACGCGAC	20	7.0264586E-4	45.000004	18
CGGGTTG	40	6.7921064E-9	45.000004	6
ACAACCG	30	2.1610977E-6	45.000004	1
TTTAGCG	30	2.1610977E-6	45.000004	1
CTTCGCG	25	3.8851475E-5	45.000004	1
CGTGGTC	20	7.0264586E-4	45.000004	18
AGCGTAT	20	7.0264586E-4	45.000004	12
AGCCGTA	25	3.8851475E-5	45.000004	25
TCGTTGG	30	2.1610977E-6	45.000004	2
CGAACGC	20	7.0264586E-4	45.000004	33
CGAACCG	30	2.1610977E-6	45.000004	1
CTCGTTG	20	7.0264586E-4	45.000004	1
CGTATTC	20	7.0264586E-4	45.000004	14
CATATCG	30	2.1610977E-6	45.000004	1
TCACCCG	30	2.1610977E-6	45.000004	1
CGAGTTT	20	7.0264586E-4	45.000004	15
ATAGGGC	50	2.1827873E-11	45.000004	4
CGAAACT	20	7.0264586E-4	45.000004	12
CGAAAAC	20	7.0264586E-4	45.000004	29
>>END_MODULE