##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554228_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446714
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25863975608555	34.0	31.0	34.0	30.0	34.0
2	32.433731649332685	34.0	31.0	34.0	30.0	34.0
3	32.53244581544344	34.0	31.0	34.0	31.0	34.0
4	36.06296198462551	37.0	35.0	37.0	35.0	37.0
5	35.89004374163335	37.0	35.0	37.0	35.0	37.0
6	35.85301557596135	37.0	35.0	37.0	35.0	37.0
7	35.840940736130946	37.0	35.0	37.0	35.0	37.0
8	35.960941452472944	37.0	35.0	37.0	35.0	37.0
9	37.5481739994717	39.0	37.0	39.0	35.0	39.0
10	37.220537077414185	39.0	37.0	39.0	34.0	39.0
11	37.19521214916031	39.0	37.0	39.0	34.0	39.0
12	37.127766311331186	39.0	37.0	39.0	33.0	39.0
13	37.088085441692	39.0	37.0	39.0	33.0	39.0
14	38.33453619094096	40.0	38.0	41.0	34.0	41.0
15	38.36180643543744	40.0	38.0	41.0	34.0	41.0
16	38.34159887534306	40.0	38.0	41.0	34.0	41.0
17	38.27666471164996	40.0	38.0	41.0	34.0	41.0
18	38.1935376997363	40.0	37.0	41.0	33.0	41.0
19	38.138650232587295	40.0	37.0	41.0	34.0	41.0
20	38.03296964053063	40.0	37.0	41.0	33.0	41.0
21	37.979326817605894	40.0	37.0	41.0	33.0	41.0
22	37.89063696235175	40.0	37.0	41.0	33.0	41.0
23	37.82580129568359	40.0	37.0	41.0	33.0	41.0
24	37.73606155168631	40.0	36.0	41.0	33.0	41.0
25	37.641475306348134	40.0	36.0	41.0	33.0	41.0
26	37.56959934096536	40.0	36.0	41.0	33.0	41.0
27	37.46313524984666	40.0	36.0	41.0	32.0	41.0
28	37.353597603836015	39.0	36.0	41.0	32.0	41.0
29	37.24245266546381	39.0	35.0	41.0	32.0	41.0
30	37.20855849603997	39.0	35.0	41.0	32.0	41.0
31	37.183159695017395	39.0	35.0	41.0	32.0	41.0
32	37.11019354665401	39.0	35.0	41.0	31.0	41.0
33	37.00849984553876	39.0	35.0	41.0	31.0	41.0
34	36.88281092600635	39.0	35.0	41.0	31.0	41.0
35	36.81715594317617	39.0	35.0	41.0	31.0	41.0
36	36.688498681483004	39.0	35.0	41.0	31.0	41.0
37	36.59135822920258	39.0	35.0	41.0	30.0	41.0
38	36.5235833217674	39.0	35.0	41.0	30.0	41.0
39	36.46508280465802	39.0	35.0	41.0	30.0	41.0
40	36.34108400453086	39.0	35.0	40.0	30.0	41.0
41	36.25622881754322	39.0	35.0	40.0	30.0	41.0
42	36.115143022157355	38.0	35.0	40.0	30.0	41.0
43	36.04418486996154	38.0	35.0	40.0	29.0	41.0
44	35.932316873883515	38.0	35.0	40.0	29.0	41.0
45	35.78123810760352	38.0	35.0	40.0	29.0	41.0
46	35.668035924551276	38.0	34.0	40.0	28.0	41.0
47	35.497557721495184	38.0	34.0	40.0	28.0	41.0
48	35.39834659312222	38.0	34.0	40.0	27.0	41.0
49	35.251223377821155	38.0	34.0	40.0	27.0	41.0
50	35.10866460419866	37.0	34.0	40.0	26.0	41.0
51	34.013556772342035	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	4.0
11	7.0
12	7.0
13	12.0
14	19.0
15	28.0
16	60.0
17	89.0
18	190.0
19	290.0
20	505.0
21	755.0
22	1165.0
23	1554.0
24	2010.0
25	2606.0
26	3264.0
27	4042.0
28	5005.0
29	6331.0
30	7947.0
31	10274.0
32	12864.0
33	17669.0
34	26875.0
35	35893.0
36	39853.0
37	56041.0
38	88090.0
39	123207.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.63576695603899	31.00999744803163	19.29870118241201	28.055534413517375
2	22.622080346709527	32.17450091109748	18.910309504515194	26.293109237677797
3	21.179322788182148	32.46641027592598	20.314115966815457	26.040150969076407
4	21.819329593431146	31.807823350062904	18.825020035190303	27.54782702131565
5	18.4925925760106	34.610735280291195	16.540560627157422	30.356111516540786
6	20.117793487555797	38.83536222280922	18.496621999758233	22.550222289876746
7	82.44827786906164	8.184207345191778	5.01484171080378	4.352673074942805
8	84.47686886911984	7.115066910819897	4.604288202294981	3.8037760177652813
9	74.3113043244671	12.987280452369973	7.395783431904977	5.305631791257941
10	33.54069941841984	37.293883782464846	14.378327072802733	14.787089726312585
11	25.908075412904008	30.13852263416862	25.230908366426842	18.722493586500537
12	27.795636581795062	25.163527447091425	27.690199993732005	19.3506359773815
13	24.10602757021271	28.104111355363838	26.846931146102428	20.94292992832103
14	19.839091678344534	29.78527648562615	27.305613882707952	23.070017953321365
15	20.52946628043894	30.712267804456545	27.737209937454388	21.02105597765013
16	21.563461185456468	30.283134175333657	26.479358157568377	21.674046481641497
17	20.713924345330568	29.467175866438033	27.299345890211633	22.519553898019762
18	21.866339537153525	29.182206064730455	27.016166943503002	21.93528745461302
19	22.777213160993387	30.10292939106453	26.292437667053193	20.827419780888892
20	23.52981997430123	28.541079975107113	26.495475852558908	21.433624198032746
21	23.16247084264205	29.90056277618342	26.627551408731314	20.309414972443218
22	22.095121263269117	30.180831583518763	25.188151703326962	22.535895449885164
23	22.148399199487816	29.89384706993736	25.675488119915652	22.28226561065917
24	20.39022730427074	29.753488809394824	28.08821751724817	21.768066369086263
25	20.349709209919546	32.78473475198897	24.904301185993724	21.961254852097763
26	23.031290713969117	30.554000993924525	24.427486042523853	21.98722224958251
27	21.376092981191544	29.496501117045803	25.668772413669593	23.458633488093053
28	21.55719319296015	29.94443872365764	27.541782885694204	20.956585197688007
29	21.046127947635398	30.981791481798197	24.67551945987813	23.29656111068827
30	20.518273436695512	31.352722323455275	25.677726688664336	22.451277551184877
31	22.22316739569389	31.4874841621261	23.960968315297933	22.328380126882077
32	22.207049700703358	30.48505307646503	24.993620079066247	22.314277143765363
33	20.73586231906768	31.571206633326916	24.929373155978993	22.76355789162641
34	20.43634182049365	30.356111516540786	26.3960834001173	22.811463262848267
35	22.483289084291066	29.119749996642145	26.726720004298055	21.670240914768733
36	21.946704155231313	32.23337526918789	24.603885259920215	21.216035315660577
37	20.4757406304705	32.80197173135384	25.495059478771655	21.227228159404003
38	20.756457151555583	30.883294456856063	27.48469938260274	20.875549008985615
39	22.129371365123994	30.147029195413623	25.959786350998627	21.76381308846376
40	22.211302981325858	29.993687236128707	26.848274287351636	20.946735495193796
41	20.116450346306586	30.69547853884141	27.211370138388318	21.97670097646369
42	19.748877357772535	30.663019291985478	26.64008739372395	22.94801595651804
43	21.754411099719285	28.952081197365654	26.51696611254628	22.776541590368783
44	22.023934777060937	29.063114207300423	25.79816168734358	23.11478932829506
45	20.949421777692216	30.86963918748909	25.672130266792625	22.508808768026075
46	19.41891232421639	31.575907627699156	26.574049615637747	22.43113043244671
47	20.20464995500477	30.995222894290308	26.802607484878465	21.997519665826456
48	20.326651951808092	29.12534641851386	27.955246533576293	22.592755096101758
49	19.770815331509645	30.87993660373304	26.844468720478876	22.504779344278443
50	19.81603442023308	29.619398541348602	27.71348110871833	22.851085929699988
51	20.619904457885806	27.933980130463787	27.895700604861272	23.55041480678913
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5876.0
1	4609.5
2	3343.0
3	2193.5
4	1044.0
5	927.0
6	810.0
7	865.5
8	921.0
9	1033.5
10	1146.0
11	1252.0
12	1358.0
13	1638.0
14	1918.0
15	1706.5
16	1495.0
17	1447.5
18	1400.0
19	1526.0
20	1652.0
21	1696.0
22	1740.0
23	2698.5
24	3657.0
25	3760.5
26	4881.5
27	5899.0
28	6802.0
29	7705.0
30	9338.5
31	10972.0
32	12464.0
33	13956.0
34	15194.0
35	16432.0
36	18541.5
37	20651.0
38	21024.5
39	21398.0
40	21499.0
41	21600.0
42	22711.5
43	23823.0
44	26076.5
45	28330.0
46	29132.5
47	29935.0
48	29652.5
49	29370.0
50	29330.5
51	29291.0
52	26726.5
53	24162.0
54	23244.5
55	22327.0
56	20781.0
57	19235.0
58	18907.0
59	18579.0
60	17526.5
61	16474.0
62	15135.0
63	13796.0
64	12642.5
65	11489.0
66	10232.0
67	8975.0
68	7945.0
69	6915.0
70	5867.5
71	4820.0
72	4259.5
73	3699.0
74	3384.5
75	2396.5
76	1723.0
77	1372.0
78	1021.0
79	769.0
80	517.0
81	336.0
82	155.0
83	118.5
84	82.0
85	68.5
86	55.0
87	36.0
88	17.0
89	11.0
90	5.0
91	5.5
92	6.0
93	5.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	446714.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.82643249544146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.16936569182432	19.67182981260495
2	7.680817470626455	3.967362278298831
3	2.9038155466831466	2.249855885868772
4	1.5476704108625212	1.598832215653322
5	1.0724608389953398	1.3848918731158824
6	0.8057707931107858	1.2486110997044422
7	0.6963526989483657	1.2589014180685902
8	0.5834755995792241	1.2055274548216048
9	0.5530859277327222	1.2855812739090988
>10	6.834995163140172	37.47335551997924
>50	0.8526378606209433	15.547391590922633
>100	0.29173759793140214	10.501715532085568
>500	0.005209599963060753	0.9216358651535635
>1k	0.001736533321020251	0.552757276789181
>5k	8.682666605101255E-4	1.1317509030243396
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5047	1.1298056474612392	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1313	0.29392407670231957	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1152	0.25788311984849366	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	857	0.19184534176229084	No Hit
GTAAAGGGGAAGAAGATTGATCAAACTGAATGAAACACACACACACACACA	789	0.17662307427123394	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	666	0.1490886786624104	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	651	0.14573082553938313	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	619	0.13856740554359165	No Hit
CTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGCT	528	0.1181964299305596	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1419252586666189	0.0
2	0.0	0.0	0.0	0.22990101048993317	0.0
3	0.0	0.0	0.0	0.3122803404415353	0.0
4	0.0	0.0	0.0	0.6919416002184843	0.0
5	0.0	0.0	0.0	0.7642473708010047	0.0
6	0.0	0.0	0.0	1.2218108230321862	0.0
7	0.0	0.0	0.0	1.5401352990951704	0.0
8	0.0	0.0	0.0	1.8598029164073657	0.0
9	0.0	0.0	0.0	2.2504331630528704	0.0
10	0.0	0.0	0.0	2.9699091588801783	0.0
11	0.0	0.0	0.0	3.5230594966802027	0.0
12	0.0	0.0	0.0	3.8001943077673856	0.0
13	0.0	0.0	0.0	3.938090142686372	0.0
14	0.0	0.0	0.0	4.075314406980753	0.0
15	0.0	0.0	0.0	4.200226543157367	0.0
16	0.0	0.0	0.0	4.4265458436494045	0.0
17	0.0	0.0	0.0	4.695397950366454	0.0
18	0.0	0.0	0.0	5.099683466378936	0.0
19	0.0	0.0	0.0	5.246981290042399	0.0
20	0.0	0.0	0.0	5.471733592410357	0.0
21	0.0	0.0	0.0	5.662907363548042	0.0
22	0.0	0.0	0.0	5.868184117802442	0.0
23	0.0	0.0	0.0	6.081519719552107	0.0
24	0.0	0.0	0.0	6.246726093205049	0.0
25	0.0	0.0	0.0	6.397381769991538	0.0
26	0.0	0.0	0.0	6.530352753663418	0.0
27	0.0	0.0	0.0	6.704737259185967	0.0
28	0.0	0.0	0.0	6.869495919089172	0.0
29	0.0	0.0	0.0	7.020151595875661	0.0
30	0.0	0.0	0.0	7.244903898243619	0.0
31	2.238568748684841E-4	0.0	0.0	7.405409277524322	0.0
32	2.238568748684841E-4	0.0	0.0	7.565914656805025	0.0
33	2.238568748684841E-4	0.0	0.0	7.72910631858415	0.0
34	2.238568748684841E-4	0.0	0.0	7.896103547236039	0.0
35	2.238568748684841E-4	0.0	0.0	8.068921054634508	0.0
36	2.238568748684841E-4	0.0	0.0	8.25046898015285	0.0
37	2.238568748684841E-4	0.0	0.0	8.425525056300003	0.0
38	2.238568748684841E-4	0.0	0.0	8.610430834941372	0.0
39	2.238568748684841E-4	0.0	0.0	8.791754903584843	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	25	3.8869628E-5	45.0	1
TCGCAAT	20	7.0286513E-4	45.0	27
ATTAGCG	35	1.2097007E-7	45.0	1
CGTATAT	25	3.8869628E-5	45.0	30
CGTATAG	25	3.8869628E-5	45.0	1
ATTCGTT	20	7.0286513E-4	45.0	12
TATGCGG	40	6.7993824E-9	45.0	2
TTATCGC	20	7.0286513E-4	45.0	24
TACGCGG	40	6.7993824E-9	45.0	2
TATCTCG	20	7.0286513E-4	45.0	1
CATAGCG	20	7.0286513E-4	45.0	1
TAACACG	35	1.2097007E-7	45.0	1
TCTAACG	20	7.0286513E-4	45.0	1
CGGTGTA	25	3.8869628E-5	45.0	36
CTACATT	20	7.0286513E-4	45.0	14
CATCGCG	25	3.8869628E-5	45.0	2
CGTAACG	20	7.0286513E-4	45.0	1
CCGAAAC	25	3.8869628E-5	45.0	21
TCATACG	20	7.0286513E-4	45.0	1
ACCCGAT	20	7.0286513E-4	45.0	26
>>END_MODULE