##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554227_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	436459
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32857152676426	34.0	31.0	34.0	30.0	34.0
2	32.49561585395192	34.0	31.0	34.0	30.0	34.0
3	32.59436510645902	34.0	31.0	34.0	31.0	34.0
4	36.10129473787916	37.0	35.0	37.0	35.0	37.0
5	35.9392657729592	37.0	35.0	37.0	35.0	37.0
6	35.919884800175964	37.0	35.0	37.0	35.0	37.0
7	35.864046794773394	37.0	35.0	37.0	35.0	37.0
8	35.997103966237376	37.0	35.0	37.0	35.0	37.0
9	37.60236814912741	39.0	37.0	39.0	35.0	39.0
10	37.28661798702742	39.0	37.0	39.0	34.0	39.0
11	37.285685482485185	39.0	37.0	39.0	34.0	39.0
12	37.221118134807625	39.0	37.0	39.0	34.0	39.0
13	37.185715038525956	39.0	37.0	39.0	34.0	39.0
14	38.44727683470841	40.0	38.0	41.0	34.0	41.0
15	38.4630698416117	40.0	38.0	41.0	34.0	41.0
16	38.44610146657533	40.0	38.0	41.0	34.0	41.0
17	38.390128282381625	40.0	38.0	41.0	34.0	41.0
18	38.30847571020416	40.0	37.0	41.0	34.0	41.0
19	38.26163282232695	40.0	37.0	41.0	34.0	41.0
20	38.155874893174385	40.0	37.0	41.0	34.0	41.0
21	38.091273178007555	40.0	37.0	41.0	33.0	41.0
22	38.017532001860424	40.0	37.0	41.0	33.0	41.0
23	37.935696136406854	40.0	37.0	41.0	33.0	41.0
24	37.89689753218515	40.0	37.0	41.0	33.0	41.0
25	37.78727898840441	40.0	36.0	41.0	33.0	41.0
26	37.74710110227994	40.0	36.0	41.0	33.0	41.0
27	37.66466724251304	40.0	36.0	41.0	33.0	41.0
28	37.5178447460128	40.0	36.0	41.0	33.0	41.0
29	37.44282968159667	40.0	36.0	41.0	32.0	41.0
30	37.393631475121374	40.0	36.0	41.0	32.0	41.0
31	37.33557332991186	40.0	36.0	41.0	32.0	41.0
32	37.26963357382939	39.0	35.0	41.0	32.0	41.0
33	37.19205240354764	39.0	35.0	41.0	32.0	41.0
34	37.06314911595362	39.0	35.0	41.0	31.0	41.0
35	36.96123347210162	39.0	35.0	41.0	31.0	41.0
36	36.870885925138445	39.0	35.0	41.0	31.0	41.0
37	36.76860369473421	39.0	35.0	41.0	31.0	41.0
38	36.70694154548308	39.0	35.0	41.0	31.0	41.0
39	36.65806181107504	39.0	35.0	41.0	31.0	41.0
40	36.51457983453199	39.0	35.0	41.0	30.0	41.0
41	36.43348401568074	39.0	35.0	41.0	30.0	41.0
42	36.32123979571964	39.0	35.0	40.0	30.0	41.0
43	36.239236675151616	39.0	35.0	40.0	30.0	41.0
44	36.12884600844524	38.0	35.0	40.0	30.0	41.0
45	35.99349767102981	38.0	35.0	40.0	29.0	41.0
46	35.8426014814679	38.0	35.0	40.0	29.0	41.0
47	35.71682334423165	38.0	34.0	40.0	28.0	41.0
48	35.59897493235332	38.0	34.0	40.0	28.0	41.0
49	35.486244985210526	38.0	34.0	40.0	28.0	41.0
50	35.379994455378394	38.0	34.0	40.0	27.0	41.0
51	34.31602968434607	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	4.0
11	2.0
12	8.0
13	6.0
14	11.0
15	26.0
16	43.0
17	72.0
18	145.0
19	261.0
20	418.0
21	692.0
22	1019.0
23	1360.0
24	1946.0
25	2328.0
26	2884.0
27	3607.0
28	4523.0
29	5781.0
30	7399.0
31	9102.0
32	11930.0
33	16171.0
34	25052.0
35	34776.0
36	38138.0
37	54034.0
38	86223.0
39	128431.0
40	62.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.642811810502245	30.95640140311003	19.245335758914354	28.155451027473372
2	22.63099168535876	32.11641872432462	18.876687157327492	26.375902432989122
3	21.237962786882616	32.43168315924291	20.309582343358713	26.020771710515767
4	21.877198087334666	31.8394167607954	18.772439106536925	27.510946045333007
5	18.612286606531196	34.608978162897316	16.596060569263095	30.182674661308397
6	20.165697121608215	38.81028916805473	18.4024158053792	22.621597904957856
7	82.3550436581672	8.171901599004718	4.997032940092884	4.476021802735194
8	84.41961329701073	7.022423641166753	4.66343917756307	3.8945238842594607
9	74.2273615620253	13.042233061982914	7.363807367931467	5.366598008060322
10	33.48951447902323	37.24106960791277	14.355987618539197	14.913428294524799
11	25.95249496516282	30.103629435983674	25.143484267708992	18.800391331144507
12	27.94878785865339	25.178768223361185	27.53179565549112	19.3406482624943
13	24.036851113162978	28.203107279263346	26.84582056962968	20.914221037944
14	19.774824210292373	29.813796943126388	27.352626478088432	23.0587523684928
15	20.62324296211099	30.622807640580213	27.68942787295027	21.064521524358533
16	21.681303398486456	30.228956213527503	26.419434586066505	21.67030580191954
17	20.677314478565	29.61423638875587	27.190641045321556	22.517808087357576
18	21.74408134555594	29.294160505339562	27.097161474502762	21.86459667460174
19	22.786332736866463	30.014044847282335	26.333286746292323	20.866335669558882
20	23.61206894576581	28.46773694665478	26.512914156885298	21.407279950694107
21	22.895163119559914	30.057118767169428	26.52047500452505	20.527243108745612
22	22.131288391349475	30.111419400218576	25.209011613920207	22.54828059451174
23	22.065302811947973	30.123562579761217	25.558185304919824	22.252949303370993
24	20.436054703878256	29.735897300777392	28.139641982408424	21.688406012935925
25	20.31691407440332	32.78681388171626	24.847007393592524	22.049264650287885
26	22.984060358475826	30.686960287220565	24.348678799154104	21.98030055514951
27	21.347251402766354	29.49211724354407	25.643416678313425	23.517214675376152
28	21.574535065149302	30.15770095243769	27.388368666930912	20.879395315482096
29	21.17633042278885	30.956859636300315	24.563131932208982	23.303678008701848
30	20.419787425623028	31.570433878096228	25.53733569476171	22.472443001519043
31	22.358113820542137	31.378663287960613	23.9309992462064	22.332223645290853
32	22.14205687132125	30.50916580938874	24.905661241949414	22.443116077340598
33	20.727261896306413	31.50559388167044	24.891226896455336	22.87591732556781
34	20.411310111602692	30.468383055453092	26.253783287777317	22.8665235451669
35	22.50795607376638	29.260709482448522	26.617849557461298	21.6134848863238
36	21.88705010092586	32.278862390281795	24.537012640362555	21.297074868429792
37	20.35792594493412	32.89633161419515	25.41338361678875	21.332358824081986
38	20.822345283291213	30.84482162127485	27.416779124728784	20.916053970705153
39	22.07973715744205	30.282340380196075	25.969907826393772	21.668014635968095
40	22.230954110237157	30.170531481765757	26.73080403886734	20.867710369129746
41	20.018146034335413	30.90393370282203	27.15696090583537	21.920959357007188
42	19.645831567226246	30.70254021569036	26.629305387218498	23.02232282986489
43	21.83389505085243	29.006390061838573	26.37406950022797	22.785645387081033
44	21.98992345214556	29.156232315062812	25.723378370018718	23.130465862772905
45	20.858316588728837	30.96373313415464	25.740562114654526	22.437388162461996
46	19.504008394832045	31.636877690688014	26.616245741295288	22.24286817318465
47	20.383587003590257	31.002912071924282	26.770212093232125	21.843288831253336
48	20.366632375549592	29.13561182149984	28.036768631188725	22.460987171761836
49	19.71227537981804	31.05583800540257	26.781667922989328	22.450218691790063
50	19.756953115871138	29.730627619089077	27.61931819483617	22.893101070203617
51	20.606517450665468	27.95886898883973	27.812463484542647	23.62215007595215
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5854.0
1	4567.0
2	3280.0
3	2127.0
4	974.0
5	875.5
6	777.0
7	831.0
8	885.0
9	980.5
10	1076.0
11	1199.5
12	1323.0
13	1583.5
14	1844.0
15	1660.5
16	1477.0
17	1404.5
18	1332.0
19	1468.5
20	1605.0
21	1658.0
22	1711.0
23	2620.0
24	3529.0
25	3674.5
26	4778.5
27	5737.0
28	6682.5
29	7628.0
30	9157.5
31	10687.0
32	12206.5
33	13726.0
34	15042.5
35	16359.0
36	18125.0
37	19891.0
38	20207.5
39	20524.0
40	20772.0
41	21020.0
42	22202.0
43	23384.0
44	25514.5
45	27645.0
46	28367.0
47	29089.0
48	29034.5
49	28980.0
50	28846.0
51	28712.0
52	26285.0
53	23858.0
54	22665.5
55	21473.0
56	20102.5
57	18732.0
58	18345.0
59	17958.0
60	17140.0
61	16322.0
62	14936.0
63	13550.0
64	12396.5
65	11243.0
66	10156.5
67	9070.0
68	7928.5
69	6787.0
70	5750.5
71	4714.0
72	4118.5
73	3523.0
74	3230.0
75	2329.0
76	1721.0
77	1330.5
78	940.0
79	697.0
80	454.0
81	309.5
82	165.0
83	122.0
84	79.0
85	60.0
86	41.0
87	28.5
88	16.0
89	10.5
90	5.0
91	2.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	436459.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.55850521322969
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.55368197816911	17.092562951217065
2	8.794753140918516	4.143824754387942
3	3.3414869944975547	2.361613163394296
4	1.9104246582889624	1.800269970871323
5	1.2120260298587548	1.4276760771498784
6	0.9613980363944422	1.3589460391432355
7	0.7796968674442696	1.285794490149728
8	0.729779801527934	1.375401700704445
9	0.6229583514234934	1.320837081267181
>10	7.8129358824058786	38.91271596921879
>50	0.9474158128281045	15.551586378029942
>100	0.32445747014661114	10.68664431203854
>500	0.005989984064245129	0.9360633030043882
>1k	0.0019966613547483763	0.5618731735873075
>5k	9.983306773741882E-4	1.1841906358359533
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5035	1.153602056550558	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1308	0.29968450644848654	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1081	0.24767503935077523	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	872	0.19978967096565772	No Hit
GTAAAGGGGAAGAAGATTGATCAAACTGAATGAAACACACACACACACACA	744	0.17046274678721254	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	656	0.15030048641453148	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	643	0.14732197067765815	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	564	0.12922175966127403	No Hit
CTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGCT	501	0.11478741416719555	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1356370243253089	0.0
2	0.0	0.0	0.0	0.22109751431405927	0.0
3	0.0	0.0	0.0	0.3102238698251153	0.0
4	0.0	0.0	0.0	0.7159893598253215	0.0
5	0.0	0.0	0.0	0.7819749392268232	0.0
6	0.0	0.0	0.0	1.2237117346646535	0.0
7	0.0	0.0	0.0	1.5504319993401443	0.0
8	0.0	0.0	0.0	1.8831092954893816	0.0
9	0.0	0.0	0.0	2.2817721710401204	0.0
10	0.0	0.0	0.0	3.0259428720681667	0.0
11	0.0	0.0	0.0	3.613856055207935	0.0
12	0.0	0.0	0.0	3.922017875676753	0.0
13	0.0	0.0	0.0	4.053530801289468	0.0
14	0.0	0.0	0.0	4.195124857088524	0.0
15	0.0	0.0	0.0	4.31105785423144	0.0
16	0.0	0.0	0.0	4.55117204594246	0.0
17	0.0	0.0	0.0	4.830236058827977	0.0
18	0.0	0.0	0.0	5.222025436524393	0.0
19	0.0	0.0	0.0	5.371867689748636	0.0
20	0.0	0.0	0.0	5.595256370014137	0.0
21	0.0	0.0	0.0	5.80375247159527	0.0
22	0.0	0.0	0.0	6.015227088913277	0.0
23	0.0	0.0	0.0	6.217307925830376	0.0
24	0.0	0.0	0.0	6.379522475192401	0.0
25	0.0	0.0	0.0	6.527302679060347	0.0
26	0.0	0.0	0.0	6.676457582499158	0.0
27	0.0	0.0	0.0	6.857688809258144	0.0
28	0.0	0.0	0.0	7.016237493097862	0.0
29	0.0	0.0	0.0	7.1779938092696	0.0
30	0.0	0.0	0.0	7.413754785672881	0.0
31	0.0	0.0	0.0	7.581009900128076	0.0
32	0.0	0.0	0.0	7.744370032465821	0.0
33	0.0	0.0	0.0	7.913916312872457	0.0
34	0.0	0.0	0.0	8.091481674109138	0.0
35	0.0	0.0	0.0	8.269505268536106	0.0
36	0.0	0.0	0.0	8.450278262104803	0.0
37	0.0	0.0	0.0	8.626698040365762	0.0
38	0.0	0.0	0.0	8.813886298598494	0.0
39	0.0	0.0	0.0	8.99740869130892	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAT	30	2.1623964E-6	45.000004	30
TCTAACG	35	1.2096098E-7	45.000004	1
CGACTTC	30	2.1623964E-6	45.000004	17
CGTTCTA	20	7.028474E-4	45.0	39
TATGCGG	50	2.1827873E-11	45.0	2
TCGATAG	20	7.028474E-4	45.0	1
CTGCGTA	20	7.028474E-4	45.0	35
AAACGCA	25	3.886816E-5	45.0	35
AGACGCA	20	7.028474E-4	45.0	41
ATATCGT	20	7.028474E-4	45.0	44
CGTAGTA	20	7.028474E-4	45.0	26
CAAGGCG	20	7.028474E-4	45.0	1
AGCGATA	20	7.028474E-4	45.0	35
CCGACGG	25	3.886816E-5	45.0	2
AGTTATC	40	6.7975634E-9	45.0	25
TATAGGC	20	7.028474E-4	45.0	44
CGTAACG	20	7.028474E-4	45.0	1
CGAGACA	110	0.0	45.0	22
ACGTAGA	20	7.028474E-4	45.0	45
ACGTAAA	25	3.886816E-5	45.0	20
>>END_MODULE