SRR3554226_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554226_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	239639
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8546	3.56619748872262	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1633	0.6814416685097167	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1533	0.6397122338183685	No Hit
CTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCT	1023	0.42689211689249246	TruSeq Adapter, Index 13 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	867	0.3617941987739892	No Hit
TTAGCAGGGAGTGGTGTGTGTGTCTGTCAATGTGTGTATATGAGTTTGTGA	460	0.19195539958020189	No Hit
AATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT	459	0.1915381052332884	TruSeq Adapter, Index 13 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGT	422	0.17609821439748954	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCC	393	0.16399667833699857	No Hit
TAGCACGGGAGCTGTTTGGAACAGAGTTGTTTCCTTTCTGAATTTCAAAGA	377	0.15731996878638285	No Hit
TAGTGCGGGACCGTTTAGTTGCTCATCCCAGTTCTGGATAAATAGACAAAA	294	0.1226845379925638	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	293	0.12226724364565032	No Hit
TATGATGGGATTTATATTTTTATTTTATGGTGGTGCTGGAGACAGAACCAG	291	0.12143265495182336	No Hit
GAACACGGGATGATGGTTTTGCTTATTTTGCTTTTGATCTGACTTCTAAAA	287	0.11976347756416944	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	286	0.11934618321725596	No Hit
GGGAAGGGATTCTGTCATATATATGCTGGGTTCTGCTTGCTTTATTCTTTT	273	0.11392135670738068	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	248	0.10348899803454363	No Hit
CAAACCGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTA	244	0.1018198206468897	No Hit
AAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT	244	0.1018198206468897	TruSeq Adapter, Index 19 (95% over 21bp)
GTTTACGGGATTGATAAGACAGAAATGCTTCTTTTTCTCAGTATTTTTTTC	241	0.10056793760614925	No Hit
ATTGCAGGGATCAACTTATACATCTTCTATGAAAAAACTTTCTACCCCTAA	240	0.10015064325923577	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACC	20	7.022099E-4	45.000004	43
AATCCCG	20	7.022099E-4	45.000004	1
TCACCGG	20	7.022099E-4	45.000004	2
CCCTCAA	20	7.022099E-4	45.000004	42
GTCGAGG	40	6.7757355E-9	45.000004	2
AGTCACG	20	7.022099E-4	45.000004	1
GGTAAAC	20	7.022099E-4	45.000004	8
CAGCCTA	20	7.022099E-4	45.000004	38
TGGACGG	20	7.022099E-4	45.000004	2
TCCGAAG	20	7.022099E-4	45.000004	1
CTTCACG	20	7.022099E-4	45.000004	1
GGTCCTA	20	7.022099E-4	45.000004	9
TCGGGCA	20	7.022099E-4	45.000004	12
GAATCAC	20	7.022099E-4	45.000004	9
CCTACCG	20	7.022099E-4	45.000004	1
TGTATCG	20	7.022099E-4	45.000004	24
CGATTTG	20	7.022099E-4	45.000004	1
CTAAGCG	20	7.022099E-4	45.000004	1
CCGCTGG	20	7.022099E-4	45.000004	2
ATCGTAG	20	7.022099E-4	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content