##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554226_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	239639
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.056835490049615	33.0	31.0	34.0	30.0	34.0
2	32.25039329992197	34.0	31.0	34.0	30.0	34.0
3	32.34768964984831	34.0	31.0	34.0	30.0	34.0
4	35.92176148289719	37.0	35.0	37.0	35.0	37.0
5	35.71357750616552	37.0	35.0	37.0	33.0	37.0
6	35.68134569080993	37.0	35.0	37.0	33.0	37.0
7	35.63179616005742	37.0	35.0	37.0	35.0	37.0
8	35.80791941211572	37.0	35.0	37.0	35.0	37.0
9	37.45982915969437	39.0	37.0	39.0	35.0	39.0
10	37.04966220022617	39.0	37.0	39.0	33.0	39.0
11	37.03342945013124	39.0	37.0	39.0	33.0	39.0
12	37.05092660209732	39.0	37.0	39.0	33.0	39.0
13	37.04899035632764	39.0	37.0	39.0	33.0	39.0
14	38.25832606545679	40.0	38.0	41.0	33.0	41.0
15	38.24265666273019	40.0	38.0	41.0	33.0	41.0
16	38.2397481211322	40.0	38.0	41.0	33.0	41.0
17	38.16384645237211	40.0	37.0	41.0	33.0	41.0
18	37.98753124491422	40.0	37.0	41.0	33.0	41.0
19	37.861124441347194	40.0	36.0	41.0	33.0	41.0
20	37.730891048618965	39.0	36.0	41.0	33.0	41.0
21	37.66590579997413	39.0	36.0	41.0	33.0	41.0
22	37.59589632739245	39.0	36.0	41.0	33.0	41.0
23	37.478619924135884	39.0	36.0	41.0	33.0	41.0
24	37.430893969679396	39.0	35.0	41.0	32.0	41.0
25	37.383226436431464	39.0	35.0	41.0	32.0	41.0
26	37.24590738569265	39.0	35.0	41.0	32.0	41.0
27	37.17845592745755	39.0	35.0	41.0	32.0	41.0
28	37.1793280726426	39.0	35.0	41.0	32.0	41.0
29	37.02477476537625	39.0	35.0	41.0	31.0	41.0
30	36.95973944140979	39.0	35.0	41.0	31.0	41.0
31	36.86262670099608	39.0	35.0	41.0	31.0	41.0
32	36.79051406490596	39.0	35.0	41.0	31.0	41.0
33	36.669256673579845	39.0	35.0	41.0	31.0	41.0
34	36.56673997137361	39.0	35.0	40.0	30.0	41.0
35	36.565125042251054	39.0	35.0	40.0	30.0	41.0
36	36.45436260374981	39.0	35.0	40.0	30.0	41.0
37	36.33986955378715	39.0	35.0	40.0	30.0	41.0
38	36.262832844403455	38.0	35.0	40.0	30.0	41.0
39	36.19884075630427	38.0	35.0	40.0	30.0	41.0
40	36.13893397986138	38.0	35.0	40.0	30.0	41.0
41	36.08040844770676	38.0	35.0	40.0	30.0	41.0
42	36.01849448545521	38.0	35.0	40.0	30.0	41.0
43	35.93404662847033	38.0	35.0	40.0	29.0	41.0
44	35.86253072329629	38.0	35.0	40.0	29.0	41.0
45	35.723321329165955	38.0	35.0	40.0	28.0	41.0
46	35.633519585710175	38.0	35.0	40.0	28.0	41.0
47	35.52968840631116	38.0	35.0	40.0	28.0	41.0
48	35.42581967042093	38.0	34.0	40.0	27.0	41.0
49	35.338550903650905	38.0	34.0	40.0	27.0	41.0
50	35.1899273490542	38.0	34.0	40.0	26.0	41.0
51	34.15766632309432	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	2.0
13	2.0
14	3.0
15	9.0
16	21.0
17	60.0
18	115.0
19	191.0
20	359.0
21	516.0
22	820.0
23	1089.0
24	1359.0
25	1560.0
26	1801.0
27	2210.0
28	2703.0
29	3348.0
30	4423.0
31	5578.0
32	7251.0
33	10073.0
34	14363.0
35	23109.0
36	22662.0
37	29307.0
38	45013.0
39	61665.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.975625837196784	36.210299659070515	20.35937389156189	24.454700612170807
2	20.10732810602615	37.28399801367891	20.988653766707422	21.620020113587522
3	19.05282529137578	36.51993206448032	21.583715505406047	22.84352713873785
4	19.610330538852192	35.76629847395457	18.50032757606233	26.123043411130908
5	17.24510618054657	40.420382325080645	16.61081877323808	25.723692721134704
6	18.73818535380301	40.200050909910324	19.27691235566832	21.78485138061835
7	79.88265682964793	12.02725766674039	4.17252617478791	3.917559328823773
8	80.67342961704898	11.429274867613369	3.7982131456065167	4.099082369731137
9	72.19776413688925	16.91252258605653	7.2738577610489115	3.6158555160053245
10	26.958883987998618	50.28897633523759	11.510647265261497	11.241492411502302
11	16.599551825871416	32.56231247835286	35.76588117960766	15.072254516168067
12	20.998251536686432	30.25133638514599	32.28648091504304	16.463931163124535
13	20.495411848655685	31.519076611069153	31.42894103213584	16.556570508139327
14	17.46168194659467	33.911842396271055	29.909572315023848	18.716903342110424
15	14.08618797441151	37.121670512729565	31.083421312891474	17.708720199967452
16	17.92738243775012	36.06049098852858	28.164447356231666	17.847679217489638
17	16.21188537758879	35.11907494189177	30.056877219484306	18.61216246103514
18	17.4161968627811	33.552551963578544	29.38962355876965	19.641627614870703
19	17.5493137594465	36.86378260633703	29.065385851217872	16.521517782998593
20	18.019604488417997	36.696030278877814	28.816261126110525	16.468104106593668
21	17.9887247067464	37.465938348933186	27.064876752114642	17.480460192205776
22	17.415779568434186	34.72473178405852	28.22286856479955	19.63662008270774
23	16.749777790760266	36.49572899235934	28.04969141083046	18.704801806049932
24	15.555898664240795	36.21530719123348	28.727377430217953	19.50141671430777
25	16.123418976043133	38.00716911687997	25.929836128509965	19.93957577856693
26	17.77423541243287	37.017346926001196	28.315925204161257	16.89249245740468
27	15.04596497231252	36.947658770066646	26.624213921774004	21.382162335846836
28	16.301603662175186	35.549305413559566	28.31216955503904	19.83692136922621
29	18.157311622899446	34.89874352672145	27.91573992547123	19.028204924907882
30	17.855190515734083	36.358439152224804	26.78654142272335	18.999828909317763
31	18.41645141233272	37.34617487136902	24.47639991821031	19.760973798087957
32	18.71022663255981	35.6123168599435	26.75941729017397	18.918039217322725
33	17.18251202850955	34.96217226745229	26.82868815176161	21.02662755227655
34	17.23884676534287	35.78006918740272	25.844290787392705	21.136793259861708
35	16.64712338141955	35.03478148381524	27.715021344605844	20.603073790159364
36	18.023360137540216	35.61690709775955	27.932014405000853	18.42771835969938
37	16.977620504175032	37.169659362624614	26.08715609729635	19.765564035904006
38	17.101974219555245	37.69461565104177	27.62488576567253	17.578524363730445
39	16.6425331436035	36.998985974736996	25.883933750349485	20.47454713131001
40	17.12993294079845	36.847090832460495	25.311405906384188	20.71157032035687
41	16.17850182983571	35.045213842488074	28.651012564732785	20.125271762943427
42	17.649464402705735	33.854255776397	28.033834225647748	20.46244559524952
43	15.831312933203694	35.404921569527495	28.381440416626674	20.382325080642133
44	18.31045864821669	34.937969195331306	26.736048806746815	20.01552334970518
45	18.681016028275867	35.382804969141084	27.90989780461444	18.02628119796861
46	16.728495779067682	36.20946507037669	28.907231293737663	18.154807856817964
47	16.853266788794812	34.189343136968525	28.387282537483465	20.5701075367532
48	17.581862718505754	34.606220189535094	28.7845467557451	19.027370336214055
49	17.434975108392205	33.78373303176862	27.22845613610472	21.55283572373445
50	17.11324116692191	32.97835494222559	28.317177087202	21.59122680365049
51	17.642370398808207	33.405664353465006	27.951209944958876	21.000755302767914
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9640.0
1	7319.0
2	4998.0
3	3114.0
4	1230.0
5	1003.5
6	777.0
7	839.5
8	902.0
9	975.5
10	1049.0
11	1383.0
12	1717.0
13	1815.0
14	1913.0
15	1843.0
16	1773.0
17	1639.5
18	1506.0
19	1761.0
20	2016.0
21	1955.5
22	1895.0
23	2065.0
24	2235.0
25	2472.0
26	2924.5
27	3140.0
28	4195.0
29	5250.0
30	6155.5
31	7061.0
32	8583.0
33	10105.0
34	10472.0
35	10839.0
36	12013.5
37	13188.0
38	13301.0
39	13414.0
40	14033.5
41	14653.0
42	15257.0
43	15861.0
44	16756.5
45	17652.0
46	18266.5
47	18881.0
48	19683.0
49	20485.0
50	18411.0
51	16337.0
52	14106.0
53	11875.0
54	9867.0
55	7859.0
56	6649.5
57	5440.0
58	4619.0
59	3798.0
60	3414.0
61	3030.0
62	2491.5
63	1953.0
64	1772.5
65	1592.0
66	1170.0
67	748.0
68	743.0
69	738.0
70	591.5
71	445.0
72	368.5
73	292.0
74	302.0
75	231.5
76	151.0
77	104.0
78	57.0
79	57.0
80	57.0
81	41.0
82	25.0
83	23.0
84	21.0
85	17.0
86	13.0
87	7.5
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	239639.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.894386973739667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.37556561085974	23.90470666293884
2	8.81880191801175	5.449029581996253
3	3.380833389613021	3.133463250973339
4	1.858580401161613	2.2967880854118072
5	1.2750726007969204	1.9696293174316366
6	0.8387924630242454	1.5548387365996352
7	0.642939150401837	1.3904247639157232
8	0.4970622003106639	1.228514557313292
9	0.45113797528196126	1.2543868068219282
>10	3.948132639967583	26.12053964504943
>50	0.6523941379077464	14.134594118653473
>100	0.25393395015870873	11.887046766177459
>500	0.0013507125008441953	0.3617941987739892
>1k	0.004052137502532586	1.7480460192205776
>5k	0.0013507125008441953	3.56619748872262
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8546	3.56619748872262	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1633	0.6814416685097167	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1533	0.6397122338183685	No Hit
CTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCT	1023	0.42689211689249246	TruSeq Adapter, Index 13 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	867	0.3617941987739892	No Hit
TTAGCAGGGAGTGGTGTGTGTGTCTGTCAATGTGTGTATATGAGTTTGTGA	460	0.19195539958020189	No Hit
AATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT	459	0.1915381052332884	TruSeq Adapter, Index 13 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGT	422	0.17609821439748954	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCC	393	0.16399667833699857	No Hit
TAGCACGGGAGCTGTTTGGAACAGAGTTGTTTCCTTTCTGAATTTCAAAGA	377	0.15731996878638285	No Hit
TAGTGCGGGACCGTTTAGTTGCTCATCCCAGTTCTGGATAAATAGACAAAA	294	0.1226845379925638	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	293	0.12226724364565032	No Hit
TATGATGGGATTTATATTTTTATTTTATGGTGGTGCTGGAGACAGAACCAG	291	0.12143265495182336	No Hit
GAACACGGGATGATGGTTTTGCTTATTTTGCTTTTGATCTGACTTCTAAAA	287	0.11976347756416944	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	286	0.11934618321725596	No Hit
GGGAAGGGATTCTGTCATATATATGCTGGGTTCTGCTTGCTTTATTCTTTT	273	0.11392135670738068	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	248	0.10348899803454363	No Hit
CAAACCGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTA	244	0.1018198206468897	No Hit
AAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT	244	0.1018198206468897	TruSeq Adapter, Index 19 (95% over 21bp)
GTTTACGGGATTGATAAGACAGAAATGCTTCTTTTTCTCAGTATTTTTTTC	241	0.10056793760614925	No Hit
ATTGCAGGGATCAACTTATACATCTTCTATGAAAAAACTTTCTACCCCTAA	240	0.10015064325923577	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5295465262332091	0.0
2	0.0	0.0	0.0	0.7131560388751413	0.0
3	0.0	0.0	0.0	0.9505965222689128	0.0
4	0.0	0.0	0.0	2.087723617608152	0.0
5	0.0	0.0	0.0	2.265073715046382	0.0
6	0.0	0.0	0.0	3.2849410989029333	0.0
7	0.0	0.0	0.0	4.113270377526196	0.0
8	0.0	0.0	0.0	4.968723788698835	0.0
9	0.0	0.0	0.0	6.027399546818339	0.0
10	0.0	0.0	0.0	7.637738431557468	0.0
11	0.0	0.0	0.0	8.89880194793001	0.0
12	0.0	0.0	0.0	9.550198423461957	0.0
13	0.0	0.0	0.0	9.81893598287424	0.0
14	0.0	0.0	0.0	10.076406594919858	0.0
15	0.0	0.0	0.0	10.30424930833462	0.0
16	0.0	0.0	0.0	10.72738577610489	0.0
17	0.0	0.0	0.0	11.31076327308994	0.0
18	0.0	0.0	0.0	12.129077487387278	0.0
19	0.0	0.0	0.0	12.456236255367449	0.0
20	0.0	0.0	0.0	12.782143140306879	0.0
21	0.0	0.0	0.0	13.12140344434754	0.0
22	0.0	0.0	0.0	13.510321775670905	0.0
23	0.0	0.0	0.0	13.9063341108918	0.0
24	0.0	0.0	0.0	14.19635368199667	0.0
25	0.0	0.0	0.0	14.41918886324847	0.0
26	0.0	0.0	0.0	14.659550407070636	0.0
27	0.0	0.0	0.0	14.91785560781008	0.0
28	0.0	0.0	0.0	15.171153276386566	0.0
29	0.0	0.0	0.0	15.461590141838348	0.0
30	0.0	0.0	0.0	15.83673775971357	0.0
31	0.0	0.0	0.0	16.11382120606412	0.0
32	0.0	0.0	0.0	16.383810648517144	0.0
33	0.0	0.0	0.0	16.67424751396893	0.0
34	0.0	0.0	0.0	16.929214359933066	0.0
35	0.0	0.0	0.0	17.219233931037937	0.0
36	0.0	0.0	0.0	17.517182094734164	0.0
37	0.0	0.0	0.0	17.777573767208175	0.0
38	0.0	0.0	0.0	18.04881509270194	0.0
39	0.0	0.0	0.0	18.317552652114223	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACC	20	7.022099E-4	45.000004	43
AATCCCG	20	7.022099E-4	45.000004	1
TCACCGG	20	7.022099E-4	45.000004	2
CCCTCAA	20	7.022099E-4	45.000004	42
GTCGAGG	40	6.7757355E-9	45.000004	2
AGTCACG	20	7.022099E-4	45.000004	1
GGTAAAC	20	7.022099E-4	45.000004	8
CAGCCTA	20	7.022099E-4	45.000004	38
TGGACGG	20	7.022099E-4	45.000004	2
TCCGAAG	20	7.022099E-4	45.000004	1
CTTCACG	20	7.022099E-4	45.000004	1
GGTCCTA	20	7.022099E-4	45.000004	9
TCGGGCA	20	7.022099E-4	45.000004	12
GAATCAC	20	7.022099E-4	45.000004	9
CCTACCG	20	7.022099E-4	45.000004	1
TGTATCG	20	7.022099E-4	45.000004	24
CGATTTG	20	7.022099E-4	45.000004	1
CTAAGCG	20	7.022099E-4	45.000004	1
CCGCTGG	20	7.022099E-4	45.000004	2
ATCGTAG	20	7.022099E-4	45.000004	1
>>END_MODULE