Basic Statistics
Measure | Value |
---|---|
Filename | SRR3554222_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 409982 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 40 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 10617 | 2.589625886014508 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1992 | 0.48587498963369125 | No Hit |
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT | 1694 | 0.41318887170656277 | TruSeq Adapter, Index 20 (95% over 22bp) |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1627 | 0.39684669083032914 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 923 | 0.2251318350561732 | No Hit |
GTACGGGAACATTTACTAATTATTTTTTTAAACTTCAGTTTTACTGCATTT | 729 | 0.17781268445931772 | No Hit |
AAAGAAGGAAAGTAAAAAAGCGCAATGTGTGTAAAAAAAAATTAAAATTAA | 630 | 0.15366528286607706 | No Hit |
TAAGCGGGATTTACGCTTGTGATCAAATAATGATGTTAAATTCTTAAATCC | 417 | 0.10171178246849862 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTTCGTT | 30 | 2.1620726E-6 | 45.000004 | 37 |
TAGCGGA | 30 | 2.1620726E-6 | 45.000004 | 3 |
ATTACGT | 30 | 2.1620726E-6 | 45.000004 | 21 |
TACGCTT | 60 | 0.0 | 45.000004 | 12 |
GAACGTA | 30 | 2.1620726E-6 | 45.000004 | 9 |
ACGCTTG | 60 | 0.0 | 45.000004 | 13 |
TTACGTA | 30 | 2.1620726E-6 | 45.000004 | 22 |
AACCTCG | 30 | 2.1620726E-6 | 45.000004 | 13 |
CTACACG | 30 | 2.1620726E-6 | 45.000004 | 1 |
TTCGGGA | 75 | 0.0 | 45.000004 | 4 |
TTACGCT | 60 | 0.0 | 45.000004 | 11 |
CTCTACT | 30 | 2.1620726E-6 | 45.000004 | 23 |
CGATAAG | 30 | 2.1620726E-6 | 45.000004 | 1 |
CGCTTGT | 60 | 0.0 | 45.000004 | 14 |
TAATCCG | 30 | 2.1620726E-6 | 45.000004 | 1 |
TGATCGA | 25 | 3.8864007E-5 | 45.0 | 33 |
GCCCCAA | 25 | 3.8864007E-5 | 45.0 | 29 |
CGGGTAT | 45 | 3.8380676E-10 | 45.0 | 6 |
CGTATCT | 20 | 7.0279714E-4 | 45.0 | 7 |
GATCGGC | 25 | 3.8864007E-5 | 45.0 | 23 |