FastQCFastQC Report
Sat 18 Jun 2016
SRR3554222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409982
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA106172.589625886014508No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19920.48587498963369125No Hit
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT16940.41318887170656277TruSeq Adapter, Index 20 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16270.39684669083032914No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9230.2251318350561732No Hit
GTACGGGAACATTTACTAATTATTTTTTTAAACTTCAGTTTTACTGCATTT7290.17781268445931772No Hit
AAAGAAGGAAAGTAAAAAAGCGCAATGTGTGTAAAAAAAAATTAAAATTAA6300.15366528286607706No Hit
TAAGCGGGATTTACGCTTGTGATCAAATAATGATGTTAAATTCTTAAATCC4170.10171178246849862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTT302.1620726E-645.00000437
TAGCGGA302.1620726E-645.0000043
ATTACGT302.1620726E-645.00000421
TACGCTT600.045.00000412
GAACGTA302.1620726E-645.0000049
ACGCTTG600.045.00000413
TTACGTA302.1620726E-645.00000422
AACCTCG302.1620726E-645.00000413
CTACACG302.1620726E-645.0000041
TTCGGGA750.045.0000044
TTACGCT600.045.00000411
CTCTACT302.1620726E-645.00000423
CGATAAG302.1620726E-645.0000041
CGCTTGT600.045.00000414
TAATCCG302.1620726E-645.0000041
TGATCGA253.8864007E-545.033
GCCCCAA253.8864007E-545.029
CGGGTAT453.8380676E-1045.06
CGTATCT207.0279714E-445.07
GATCGGC253.8864007E-545.023