##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554222_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	409982
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06960305574391	33.0	31.0	34.0	30.0	34.0
2	32.27315589464904	34.0	31.0	34.0	30.0	34.0
3	32.38868779604958	34.0	31.0	34.0	30.0	34.0
4	35.95117590528365	37.0	35.0	37.0	35.0	37.0
5	35.753296486187196	37.0	35.0	37.0	35.0	37.0
6	35.73121502895249	37.0	35.0	37.0	33.0	37.0
7	35.806825665517025	37.0	35.0	37.0	35.0	37.0
8	35.950663687674094	37.0	35.0	37.0	35.0	37.0
9	37.573264192086484	39.0	37.0	39.0	35.0	39.0
10	37.07064456488334	39.0	37.0	39.0	33.0	39.0
11	37.09550175373553	39.0	37.0	39.0	33.0	39.0
12	37.151377377543405	39.0	37.0	39.0	34.0	39.0
13	37.130256938109476	39.0	37.0	39.0	33.0	39.0
14	38.32211414159646	40.0	38.0	41.0	34.0	41.0
15	38.28594670009903	40.0	38.0	41.0	33.0	41.0
16	38.26507261294398	40.0	38.0	41.0	33.0	41.0
17	38.197972106092465	40.0	38.0	41.0	33.0	41.0
18	38.03947002551332	40.0	37.0	41.0	33.0	41.0
19	37.96236907961813	40.0	37.0	41.0	33.0	41.0
20	37.898576035045444	40.0	37.0	41.0	33.0	41.0
21	37.88475835524486	40.0	37.0	41.0	33.0	41.0
22	37.81602363030572	39.0	36.0	41.0	33.0	41.0
23	37.72954178476128	39.0	36.0	41.0	33.0	41.0
24	37.61992965544829	39.0	36.0	41.0	33.0	41.0
25	37.556170758716235	39.0	36.0	41.0	33.0	41.0
26	37.45646394231942	39.0	36.0	41.0	32.0	41.0
27	37.39830285232035	39.0	36.0	41.0	32.0	41.0
28	37.32519964291115	39.0	35.0	41.0	32.0	41.0
29	37.188918050060735	39.0	35.0	41.0	32.0	41.0
30	37.10772668068354	39.0	35.0	41.0	32.0	41.0
31	37.02288637062115	39.0	35.0	41.0	31.0	41.0
32	36.944946363498886	39.0	35.0	41.0	31.0	41.0
33	36.84752257416179	39.0	35.0	41.0	31.0	41.0
34	36.69232795586148	39.0	35.0	41.0	31.0	41.0
35	36.68942538940734	39.0	35.0	41.0	31.0	41.0
36	36.583215848500664	39.0	35.0	40.0	31.0	41.0
37	36.53096233493178	39.0	35.0	40.0	30.0	41.0
38	36.44005834402486	39.0	35.0	40.0	30.0	41.0
39	36.33596596923768	39.0	35.0	40.0	30.0	41.0
40	36.28258801605924	39.0	35.0	40.0	30.0	41.0
41	36.1947426960208	38.0	35.0	40.0	30.0	41.0
42	36.08785019830139	38.0	35.0	40.0	30.0	41.0
43	36.01260543145796	38.0	35.0	40.0	30.0	41.0
44	35.93273363220824	38.0	35.0	40.0	29.0	41.0
45	35.795417847612825	38.0	35.0	40.0	28.0	41.0
46	35.647025966993674	38.0	35.0	40.0	28.0	41.0
47	35.52621822421472	38.0	34.0	40.0	27.0	41.0
48	35.40509583347561	38.0	34.0	40.0	27.0	41.0
49	35.28004888019474	38.0	34.0	40.0	27.0	41.0
50	35.133935148372366	38.0	34.0	40.0	26.0	41.0
51	34.14758940636418	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	2.0
13	5.0
14	12.0
15	12.0
16	23.0
17	49.0
18	119.0
19	256.0
20	421.0
21	714.0
22	960.0
23	1328.0
24	1823.0
25	2270.0
26	2840.0
27	3459.0
28	4323.0
29	5690.0
30	7581.0
31	9914.0
32	12580.0
33	16907.0
34	24256.0
35	37302.0
36	41121.0
37	53646.0
38	80124.0
39	102190.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.38763653038426	34.9803162090043	22.368299096057875	24.26374816455357
2	20.187471645096615	35.52326687513111	20.583830509632133	23.705430970140153
3	19.073520300891257	34.80372309028201	21.575581366986842	24.547175241839884
4	19.38450956383451	34.558834290285915	19.794771477772194	26.26188466810738
5	16.796834982999254	38.536569898190656	17.56589313677186	27.100701982038235
6	20.52846222517086	39.032689240015415	18.42446741564264	22.014381119171087
7	82.7189974193989	8.81916767077579	4.8077720485289595	3.65406286129635
8	83.74757916201199	8.844534638106063	3.8982199218502274	3.5096662780317187
9	74.18813508885755	14.788454127254367	7.191047411837593	3.8323633720504806
10	25.745520535047877	50.727349005566104	11.928816387060895	11.598314072325126
11	17.599309237966544	30.600123907878885	35.97304271894864	15.827524135205936
12	21.932182388495107	26.360425579659598	34.80445482972423	16.90293720212107
13	21.55972701240542	28.531740417872005	32.49898776043826	17.40954480928431
14	18.122015112858612	31.468942538940738	31.7840783253899	18.624964022810758
15	17.56028313438151	34.73493958271339	31.069168890341526	16.635608392563576
16	19.28913952319858	33.83611963452054	28.85614490392261	18.01859593835827
17	18.952295466630243	33.39780770863111	29.728134405900747	17.9217624188379
18	17.22099994633911	33.338780726958746	30.647686971623145	18.792532355079004
19	17.268075183788557	35.92304052373031	29.858384026615802	16.95050026586533
20	18.08615988018986	35.101297130117906	30.015708006692975	16.796834982999254
21	18.514959193330437	33.26609460903162	30.827450961261714	17.39149523637623
22	17.34320043318975	32.778024401071264	30.279378119039375	19.59939704669961
23	16.829763257899128	34.155158031328206	29.04834846407891	19.966730246693757
24	15.514339653936027	35.32911200979555	30.320599440951067	18.835948895317355
25	17.011722465864352	36.22841978428321	27.856344912703484	18.90351283714895
26	17.46223004912411	36.00109273090038	28.38124600592221	18.1554312140533
27	16.237786049143622	34.3737042114044	28.31758467444912	21.070925065002854
28	17.441009605299744	35.18642281856277	29.195428091965013	18.177139484172475
29	18.415198716041193	34.43248727992936	27.666092657726438	19.486221346303008
30	17.264172573430052	34.90689835163495	29.346654243357023	18.48227483157797
31	18.262996912059553	36.28208067671264	26.349693401173713	19.1052290100541
32	17.887614578200996	35.413018132503375	27.348273826655806	19.351093462639824
33	18.426174807674485	34.56176124805479	27.905371455332183	19.10669248893854
34	16.716831470649932	35.82523135162031	28.521739978828336	18.936197198901414
35	18.054938997321834	34.25784546638633	28.444907337395296	19.242308198896538
36	18.46715221643877	35.6588825850891	28.33099989755648	17.54296530091565
37	17.51393963637428	36.08329146157636	27.3707138362172	19.03205506583216
38	17.789805406091	34.934216624144476	29.10030196447649	18.17567600528804
39	17.326126512871294	34.111497577942444	29.356410769253284	19.205965139932974
40	17.165143835583024	35.093491909400896	28.644428291973796	19.09693596304228
41	17.170509924825968	34.06930060344113	29.422023405905623	19.33816606582728
42	17.99005810011171	34.07295930065222	27.28095379797162	20.656028801264444
43	17.32466303398686	33.7405056807372	28.503934319067664	20.430896966208273
44	18.20933601963013	32.803147455254134	27.366079486416478	21.62143703869926
45	18.887170656272716	34.53956515164081	27.497548672868565	19.075715519217916
46	16.571703147943083	35.07714972852467	29.87862881785054	18.472518305681714
47	17.097335980604026	34.48614817235879	27.884394924655233	20.532120922381957
48	17.592479669839165	34.0797888687796	29.25616246566922	19.071568995712006
49	17.62711533677088	33.531472113409855	28.388075574049594	20.453336975769666
50	18.033718553497472	32.44288773653478	28.366367303930417	21.15702640603734
51	17.622968813264972	31.641145221009705	30.36499163377904	20.37089433194628
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11967.0
1	8781.5
2	5596.0
3	3552.0
4	1508.0
5	1384.0
6	1260.0
7	1523.5
8	1787.0
9	2303.5
10	2820.0
11	3408.5
12	3997.0
13	4197.0
14	4397.0
15	4423.0
16	4449.0
17	4076.0
18	3703.0
19	3438.0
20	3173.0
21	3755.0
22	4337.0
23	4551.0
24	4765.0
25	5883.5
26	7033.0
27	7064.0
28	10063.5
29	13063.0
30	13845.5
31	14628.0
32	15872.0
33	17116.0
34	17934.5
35	18753.0
36	20623.5
37	22494.0
38	21329.5
39	20165.0
40	20862.0
41	21559.0
42	22023.0
43	22487.0
44	23547.0
45	24607.0
46	27346.0
47	30085.0
48	30610.5
49	31136.0
50	28887.0
51	26638.0
52	23253.5
53	19869.0
54	17304.5
55	14740.0
56	13331.5
57	11923.0
58	10477.5
59	9032.0
60	8138.0
61	7244.0
62	6278.0
63	5312.0
64	4673.5
65	4035.0
66	3512.5
67	2990.0
68	2232.5
69	1475.0
70	1291.0
71	1107.0
72	954.0
73	801.0
74	563.0
75	263.0
76	201.0
77	187.0
78	173.0
79	127.0
80	81.0
81	66.5
82	52.0
83	42.0
84	32.0
85	23.0
86	14.0
87	11.5
88	9.0
89	8.0
90	7.0
91	4.0
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	409982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.403371536582043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.31242246408989	22.59348189959171
2	9.414267289588878	5.724509323001237
3	3.8179955215918806	3.4823980910389283
4	2.2648114382819937	2.754316144735528
5	1.5551387418558156	2.3640730479787555
6	1.128258950222437	2.058172563185207
7	0.8245402933209643	1.7548163419303733
8	0.673565911267896	1.6382939723723435
9	0.5402903835450614	1.4783984341706624
>10	4.63860853672421	28.56515120401592
>50	0.5748084489070143	12.25793244556901
>100	0.24967238493028135	10.883274921158014
>500	0.0024084152887165404	0.5569901403952227
>1k	0.0024084152887165404	1.2967960630674051
>5k	0.0	0.0
>10k+	8.028050962388469E-4	2.591395407789693
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10617	2.589625886014508	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1992	0.48587498963369125	No Hit
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT	1694	0.41318887170656277	TruSeq Adapter, Index 20 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1627	0.39684669083032914	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	923	0.2251318350561732	No Hit
GTACGGGAACATTTACTAATTATTTTTTTAAACTTCAGTTTTACTGCATTT	729	0.17781268445931772	No Hit
AAAGAAGGAAAGTAAAAAAGCGCAATGTGTGTAAAAAAAAATTAAAATTAA	630	0.15366528286607706	No Hit
TAAGCGGGATTTACGCTTGTGATCAAATAATGATGTTAAATTCTTAAATCC	417	0.10171178246849862	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.439131474064715E-4	0.0	0.0	0.4800210740959359	0.0
2	2.439131474064715E-4	0.0	0.0	0.6649072398300413	0.0
3	2.439131474064715E-4	0.0	0.0	0.877355591221078	0.0
4	2.439131474064715E-4	0.0	0.0	1.8815460190935211	0.0
5	2.439131474064715E-4	0.0	0.0	2.0198447736729905	0.0
6	2.439131474064715E-4	0.0	0.0	2.828172944178037	0.0
7	2.439131474064715E-4	0.0	0.0	3.402832319467684	0.0
8	2.439131474064715E-4	0.0	0.0	3.9074886214516735	0.0
9	2.439131474064715E-4	0.0	0.0	4.782405081198687	0.0
10	2.439131474064715E-4	0.0	0.0	6.0346551799835115	0.0
11	2.439131474064715E-4	0.0	0.0	7.149582176778493	0.0
12	2.439131474064715E-4	0.0	0.0	7.709362850076345	0.0
13	2.439131474064715E-4	0.0	0.0	7.936689903459176	0.0
14	2.439131474064715E-4	0.0	0.0	8.197676971184102	0.0
15	2.439131474064715E-4	0.0	0.0	8.41207662775439	0.0
16	2.439131474064715E-4	0.0	0.0	8.861120732129704	0.0
17	2.439131474064715E-4	0.0	0.0	9.479196647657702	0.0
18	2.439131474064715E-4	0.0	0.0	10.262645677127288	0.0
19	2.439131474064715E-4	0.0	0.0	10.626076266762931	0.0
20	2.439131474064715E-4	0.0	0.0	10.990238595840793	0.0
21	2.439131474064715E-4	0.0	0.0	11.389768331292593	0.0
22	2.439131474064715E-4	0.0	0.0	11.872228536862593	0.0
23	2.439131474064715E-4	0.0	0.0	12.33639525637711	0.0
24	2.439131474064715E-4	0.0	0.0	12.693240191032777	0.0
25	2.439131474064715E-4	0.0	0.0	12.97374031055022	0.0
26	2.439131474064715E-4	0.0	0.0	13.278631744808308	0.0
27	2.439131474064715E-4	0.0	0.0	13.566449258747944	0.0
28	2.439131474064715E-4	0.0	0.0	13.879145913723042	0.0
29	2.439131474064715E-4	0.0	0.0	14.222331712123946	0.0
30	2.439131474064715E-4	0.0	0.0	14.650887112117116	0.0
31	2.439131474064715E-4	0.0	0.0	14.9969998682869	0.0
32	2.439131474064715E-4	0.0	0.0	15.317257830831597	0.0
33	2.439131474064715E-4	0.0	0.0	15.654589713694747	0.0
34	2.439131474064715E-4	0.0	0.0	15.98972637823124	0.0
35	2.439131474064715E-4	0.0	0.0	16.34510783400247	0.0
36	2.439131474064715E-4	0.0	0.0	16.711709294554396	0.0
37	2.439131474064715E-4	0.0	0.0	17.09148206506627	0.0
38	2.439131474064715E-4	0.0	0.0	17.46149830968189	0.0
39	2.439131474064715E-4	0.0	0.0	17.832734120034537	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTT	30	2.1620726E-6	45.000004	37
TAGCGGA	30	2.1620726E-6	45.000004	3
ATTACGT	30	2.1620726E-6	45.000004	21
TACGCTT	60	0.0	45.000004	12
GAACGTA	30	2.1620726E-6	45.000004	9
ACGCTTG	60	0.0	45.000004	13
TTACGTA	30	2.1620726E-6	45.000004	22
AACCTCG	30	2.1620726E-6	45.000004	13
CTACACG	30	2.1620726E-6	45.000004	1
TTCGGGA	75	0.0	45.000004	4
TTACGCT	60	0.0	45.000004	11
CTCTACT	30	2.1620726E-6	45.000004	23
CGATAAG	30	2.1620726E-6	45.000004	1
CGCTTGT	60	0.0	45.000004	14
TAATCCG	30	2.1620726E-6	45.000004	1
TGATCGA	25	3.8864007E-5	45.0	33
GCCCCAA	25	3.8864007E-5	45.0	29
CGGGTAT	45	3.8380676E-10	45.0	6
CGTATCT	20	7.0279714E-4	45.0	7
GATCGGC	25	3.8864007E-5	45.0	23
>>END_MODULE