##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554221_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	399456
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.158971200833136	33.0	31.0	34.0	30.0	34.0
2	32.34762777377233	34.0	31.0	34.0	30.0	34.0
3	32.45858367379636	34.0	31.0	34.0	30.0	34.0
4	36.00117409677161	37.0	35.0	37.0	35.0	37.0
5	35.81563676600176	37.0	35.0	37.0	35.0	37.0
6	35.79915935672515	37.0	35.0	37.0	35.0	37.0
7	35.82534747256268	37.0	35.0	37.0	35.0	37.0
8	35.9736992309541	37.0	35.0	37.0	35.0	37.0
9	37.618406032203794	39.0	37.0	39.0	35.0	39.0
10	37.148471921813666	39.0	37.0	39.0	34.0	39.0
11	37.18250821116719	39.0	37.0	39.0	34.0	39.0
12	37.24285528318513	39.0	37.0	39.0	34.0	39.0
13	37.23373538011696	39.0	37.0	39.0	34.0	39.0
14	38.43899953937355	40.0	38.0	41.0	34.0	41.0
15	38.39860960906833	40.0	38.0	41.0	34.0	41.0
16	38.37807668429063	40.0	38.0	41.0	34.0	41.0
17	38.31638778739085	40.0	38.0	41.0	34.0	41.0
18	38.16991858928142	40.0	37.0	41.0	33.0	41.0
19	38.095414764079145	40.0	37.0	41.0	34.0	41.0
20	38.02395007209805	40.0	37.0	41.0	33.0	41.0
21	37.99916886966274	40.0	37.0	41.0	33.0	41.0
22	37.94358577665625	40.0	37.0	41.0	33.0	41.0
23	37.832609849395176	40.0	37.0	41.0	33.0	41.0
24	37.77726457982857	40.0	36.0	41.0	33.0	41.0
25	37.707512216614596	40.0	36.0	41.0	33.0	41.0
26	37.61448570055275	39.0	36.0	41.0	33.0	41.0
27	37.598268645357685	39.0	36.0	41.0	33.0	41.0
28	37.464724525354484	39.0	36.0	41.0	33.0	41.0
29	37.3880302211007	39.0	35.0	41.0	33.0	41.0
30	37.308774933910115	39.0	35.0	41.0	32.0	41.0
31	37.17115276776416	39.0	35.0	41.0	32.0	41.0
32	37.08805225106144	39.0	35.0	41.0	32.0	41.0
33	37.01798696226869	39.0	35.0	41.0	31.0	41.0
34	36.85790174637507	39.0	35.0	41.0	31.0	41.0
35	36.815994752863894	39.0	35.0	41.0	31.0	41.0
36	36.745674116798845	39.0	35.0	41.0	31.0	41.0
37	36.66660157814628	39.0	35.0	41.0	31.0	41.0
38	36.58486541696708	39.0	35.0	41.0	31.0	41.0
39	36.48947568693423	39.0	35.0	40.0	30.0	41.0
40	36.40511345429785	39.0	35.0	40.0	30.0	41.0
41	36.33283765921654	39.0	35.0	40.0	30.0	41.0
42	36.249737142513816	39.0	35.0	40.0	30.0	41.0
43	36.17477769766883	38.0	35.0	40.0	30.0	41.0
44	36.11589762076424	38.0	35.0	40.0	30.0	41.0
45	35.97363914924297	38.0	35.0	40.0	29.0	41.0
46	35.77359208523592	38.0	35.0	40.0	28.0	41.0
47	35.6935156813266	38.0	35.0	40.0	28.0	41.0
48	35.572636285348075	38.0	34.0	40.0	28.0	41.0
49	35.49255988143876	38.0	34.0	40.0	28.0	41.0
50	35.397768465112556	38.0	34.0	40.0	27.0	41.0
51	34.426337318753504	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	2.0
12	2.0
13	6.0
14	5.0
15	17.0
16	20.0
17	53.0
18	93.0
19	238.0
20	356.0
21	596.0
22	882.0
23	1324.0
24	1750.0
25	2146.0
26	2559.0
27	3217.0
28	3972.0
29	5221.0
30	6859.0
31	8917.0
32	11355.0
33	15299.0
34	22377.0
35	35707.0
36	38920.0
37	51655.0
38	78933.0
39	106914.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.434821357045582	35.02638588480333	22.302080829928702	24.23671192822238
2	20.1912100456621	35.357836657854676	20.500630857966833	23.950322438516384
3	19.032133701834493	34.77053793158696	21.64343507169751	24.55389329488104
4	19.443443082592328	34.531713129856605	19.928352559480896	26.096491228070175
5	16.902487382840665	38.443533205159014	17.547114075142193	27.106865336858128
6	20.429283826003363	39.05211087078427	18.537711287350795	21.980894015861573
7	82.66392293519186	8.729371945846351	4.841334214531763	3.765370904430025
8	83.65326844508532	8.877573499959945	3.8927942001121525	3.5763638548425862
9	74.07549267003124	14.880487462949613	7.207051590162621	3.836968276856525
10	25.758281262517023	50.778058159096375	11.92621965873588	11.537440919650725
11	17.631979492109267	30.534026275735	35.96315989746054	15.870834334695186
12	22.01769406392694	26.202385243931747	34.79582231835296	16.984098373788353
13	21.648692221421133	28.517033165104543	32.45213490346872	17.382139710005607
14	18.11313386205239	31.482816630617638	31.779970760233915	18.62407874709605
15	17.491288151886568	34.62483978210366	31.222462549066734	16.661409516943042
16	19.322778979412	33.90961707922775	28.812685251942643	17.954918689417607
17	18.819093967796203	33.40317832251862	29.749709605062886	18.028018104622287
18	17.276496034607067	33.356614996395095	30.59586037010334	18.771028598894496
19	17.348594087959626	35.8928142273492	29.804283826003363	16.954307858687816
20	18.089351518064568	35.064687975646876	30.097933189137226	16.748027317151326
21	18.470369702795804	33.32532243851638	30.860219899062724	17.34408795962509
22	17.400414563806777	32.92627974044701	30.165274773692218	19.508030922053994
23	16.983848033325323	34.14819154049507	28.98542017143315	19.882540254746456
24	15.60797684851398	35.16757790595209	30.14174277016743	19.082702475366496
25	17.071967876311785	36.27858888087799	27.711687895537935	18.93775534727229
26	17.490036449571416	36.13564447648802	28.202355203076184	18.171963870864374
27	16.12342786189217	34.35972923175519	28.437424897861092	21.079418008491547
28	17.320555956100296	35.1928622927181	29.197959224545382	18.288622526636225
29	18.450092125290396	34.36248297684851	27.799056717135308	19.388368180725788
30	17.36311383481535	34.908225186253304	29.310362092445725	18.41829888648562
31	18.17772170151406	36.4353020107346	26.279990787470958	19.10698550028038
32	17.885073700232315	35.526816470399744	27.23003284466875	19.358076984699192
33	18.34745253544821	34.53596891772811	27.936743971801647	19.17983457502203
34	16.687444925098134	35.91184010253946	28.398121445165426	19.002593527196986
35	18.136665865577186	34.21853721060643	28.579618280861972	19.065178642954418
36	18.504916686693903	35.53257430104943	28.40162621164784	17.56088280060883
37	17.475516702715694	35.93862653208363	27.556977489385563	19.028879275815108
38	17.813476327805816	34.88369182087639	29.165665304814546	18.137166546503245
39	17.34283625730994	33.99948930545542	29.483347352399264	19.174327084835376
40	17.17285508291276	35.02913962989666	28.730823920531922	19.067181366658655
41	17.22893134663142	34.109889449651526	29.261295361691904	19.399883842025154
42	18.06156372666827	33.895097332372025	27.392003124248976	20.651335816710727
43	17.427701674277017	33.61046022590723	28.505517503805176	20.456320596010574
44	18.287370824321076	32.650905231114315	27.46935832732516	21.592365617239444
45	18.851888568453095	34.51844508531603	27.50966314187295	19.12000320435793
46	16.660408155090924	35.07570295602019	29.936463590483058	18.32742529840583
47	17.11227269085957	34.4120503885284	27.99882840663302	20.47684851397901
48	17.69055916045822	33.912621164784106	29.347662821437154	19.049156853320515
49	17.993471120724188	33.29252783785949	28.341794840983738	20.37220620043259
50	17.939647921172792	32.41483417447729	28.324521349034686	21.320996555315226
51	17.648251622206203	31.540895618040537	30.368551229672352	20.44230153008091
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11562.0
1	8490.5
2	5419.0
3	3453.0
4	1487.0
5	1367.0
6	1247.0
7	1517.5
8	1788.0
9	2331.0
10	2874.0
11	3348.0
12	3822.0
13	4111.5
14	4401.0
15	4255.0
16	4109.0
17	3906.0
18	3703.0
19	3396.5
20	3090.0
21	3706.5
22	4323.0
23	4478.0
24	4633.0
25	5771.5
26	6787.0
27	6664.0
28	9660.5
29	12657.0
30	13372.5
31	14088.0
32	15287.0
33	16486.0
34	17210.5
35	17935.0
36	20023.0
37	22111.0
38	20930.0
39	19749.0
40	20382.5
41	21016.0
42	21641.0
43	22266.0
44	22921.5
45	23577.0
46	26313.0
47	29049.0
48	30021.0
49	30993.0
50	28503.5
51	26014.0
52	22615.0
53	19216.0
54	16914.0
55	14612.0
56	13114.0
57	11616.0
58	10239.5
59	8863.0
60	7994.0
61	7125.0
62	6154.0
63	5183.0
64	4535.5
65	3888.0
66	3395.0
67	2902.0
68	2182.0
69	1462.0
70	1243.5
71	1025.0
72	891.5
73	758.0
74	546.5
75	247.5
76	160.0
77	150.0
78	140.0
79	113.0
80	86.0
81	63.0
82	40.0
83	36.5
84	33.0
85	22.5
86	12.0
87	13.0
88	14.0
89	9.5
90	5.0
91	5.5
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	399456.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.117898181505964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.55914164698939	22.154467384430014
2	9.691886826645485	5.837985212631753
3	3.9813482575273818	3.5972952433597762
4	2.371308690074318	2.8567533473831435
5	1.5643282449549267	2.35571394020032
6	1.1417638115839455	2.0632515734768058
7	0.8970184228191317	1.8911416667781173
8	0.6590206690170336	1.5878653927170372
9	0.5575023757698676	1.5111719810446096
>10	4.717962063181287	28.4982120594021
>50	0.6115876537852281	12.834169424865763
>100	0.24130669333022597	10.377954191461301
>500	0.0024962761378988898	0.5608621255340464
>1k	0.0024962761378988898	1.3114349878997607
>5k	0.0	0.0
>10k+	8.320920459662966E-4	2.56172146881547
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10222	2.558980213089802	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1868	0.46763598493951775	No Hit
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT	1825	0.45687134502923976	TruSeq Adapter, Index 20 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1540	0.3855243130657694	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	930	0.23281663061763996	No Hit
GTACGGGAACATTTACTAATTATTTTTTTAAACTTCAGTTTTACTGCATTT	696	0.1742369622686854	No Hit
AAAGAAGGAAAGTAAAAAAGCGCAATGTGTGTAAAAAAAAATTAAAATTAA	612	0.1532083633741889	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	444	0.11115116558519586	No Hit
TAAGCGGGATTTACGCTTGTGATCAAATAATGATGTTAAATTCTTAAATCC	407	0.10188856845309621	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.503404630297204E-4	0.0	0.0	0.5159516943042538	0.0
2	2.503404630297204E-4	0.0	0.0	0.7189778098213571	0.0
3	2.503404630297204E-4	0.0	0.0	0.93126652247056	0.0
4	2.503404630297204E-4	0.0	0.0	1.9281222462549066	0.0
5	2.503404630297204E-4	0.0	0.0	2.0628054153648963	0.0
6	2.503404630297204E-4	0.0	0.0	2.8451193623327726	0.0
7	2.503404630297204E-4	0.0	0.0	3.413392213410238	0.0
8	2.503404630297204E-4	0.0	0.0	3.9155751822478573	0.0
9	2.503404630297204E-4	0.0	0.0	4.775244332291917	0.0
10	2.503404630297204E-4	0.0	0.0	6.052231034206521	0.0
11	2.503404630297204E-4	0.0	0.0	7.239846190819515	0.0
12	2.503404630297204E-4	0.0	0.0	7.819634703196347	0.0
13	2.503404630297204E-4	0.0	0.0	8.048195545942482	0.0
14	2.503404630297204E-4	0.0	0.0	8.30028839221341	0.0
15	2.503404630297204E-4	0.0	0.0	8.527096851718337	0.0
16	2.503404630297204E-4	0.0	0.0	8.989225346471201	0.0
17	2.503404630297204E-4	0.0	0.0	9.638107826644235	0.0
18	2.503404630297204E-4	0.0	0.0	10.42292517824241	0.0
19	2.503404630297204E-4	0.0	0.0	10.795932468156693	0.0
20	2.503404630297204E-4	0.0	0.0	11.162681246495234	0.0
21	2.503404630297204E-4	0.0	0.0	11.575993350957303	0.0
22	2.503404630297204E-4	0.0	0.0	12.065909637106465	0.0
23	2.503404630297204E-4	0.0	0.0	12.538802771769607	0.0
24	2.503404630297204E-4	0.0	0.0	12.898291676680286	0.0
25	2.503404630297204E-4	0.0	0.0	13.177170952495393	0.0
26	2.503404630297204E-4	0.0	0.0	13.458303292477769	0.0
27	2.503404630297204E-4	0.0	0.0	13.760464231354643	0.0
28	2.503404630297204E-4	0.0	0.0	14.096921813666587	0.0
29	2.503404630297204E-4	0.0	0.0	14.457412080429384	0.0
30	2.503404630297204E-4	0.0	0.0	14.886495634062324	0.0
31	2.503404630297204E-4	0.0	0.0	15.216694704798526	0.0
32	2.503404630297204E-4	0.0	0.0	15.541636625811103	0.0
33	2.503404630297204E-4	0.0	0.0	15.889860209885445	0.0
34	2.503404630297204E-4	0.0	0.0	16.229822558679803	0.0
35	2.503404630297204E-4	0.0	0.0	16.594568613314106	0.0
36	2.503404630297204E-4	0.0	0.0	16.938786749979972	0.0
37	2.503404630297204E-4	0.0	0.0	17.31880357285909	0.0
38	2.503404630297204E-4	0.0	0.0	17.69481494832973	0.0
39	2.503404630297204E-4	0.0	0.0	18.08860049667548	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	25	3.88622E-5	45.0	6
AGCGTAA	25	3.88622E-5	45.0	45
ACTATCC	35	1.2092642E-7	45.0	26
GTCGCGG	40	6.7957444E-9	45.0	2
GTACCGG	40	6.7957444E-9	45.0	2
TCCGCAA	20	7.027754E-4	45.0	1
TTTACGG	55	1.8189894E-12	45.0	2
AAATACG	25	3.88622E-5	45.0	1
GCGGTGT	20	7.027754E-4	45.0	9
GCGGTGC	20	7.027754E-4	45.0	25
CTATCCG	20	7.027754E-4	45.0	1
GCAACTC	25	3.88622E-5	45.0	9
TAGCGTA	20	7.027754E-4	45.0	44
TAGCGAG	20	7.027754E-4	45.0	1
GGCAACG	20	7.027754E-4	45.0	1
GGCCGCT	20	7.027754E-4	45.0	21
ACCGATA	20	7.027754E-4	45.0	32
CCGTGCG	20	7.027754E-4	45.0	1
TGAACCG	20	7.027754E-4	45.0	45
ATAGACG	25	3.88622E-5	45.0	1
>>END_MODULE