SRR3554219_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554219_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	707309
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8158	1.1533855782974627	No Hit
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT	2119	0.29958617803534243	TruSeq Adapter, Index 13 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1862	0.26325128055771946	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1523	0.21532314730902616	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1063	0.15028792225180226	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	900	0.12724283163369898	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	895	0.12653592701351177	No Hit
GTAGACGGGACTGAAGTCAATGTTCAAGAGTTAACAATGTGTTCCTTTTTA	819	0.11579097678666608	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	787	0.1112667872174679	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCC	65	0.0	45.000004	6
ACGTTAG	25	3.889278E-5	45.000004	1
ACGCACG	50	2.1827873E-11	45.000004	1
TAGGTAC	25	3.889278E-5	45.000004	23
TAACGCG	30	2.1643136E-6	45.000004	1
CGTAGCG	30	2.1643136E-6	45.000004	1
CTACGCG	30	2.1643136E-6	45.000004	1
ATGGTCG	25	3.889278E-5	45.000004	44
CGTAAGC	30	2.1643136E-6	45.000004	1
GTATACG	30	2.1643136E-6	45.000004	1
TTGCACG	30	2.1643136E-6	45.000004	1
GCGCTAC	30	2.1643136E-6	45.000004	9
AGATCGC	35	1.2110468E-7	45.0	30
CTCAACG	20	7.0314447E-4	45.0	1
ACGTTAC	20	7.0314447E-4	45.0	12
CAGCCGA	20	7.0314447E-4	45.0	42
TTTCGCG	35	1.2110468E-7	45.0	1
CGGTTAT	20	7.0314447E-4	45.0	33
TAGAACG	20	7.0314447E-4	45.0	1
ATTACGC	35	1.2110468E-7	45.0	1