##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554219_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	707309
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17641653082316	33.0	31.0	34.0	30.0	34.0
2	32.351692117589344	34.0	31.0	34.0	30.0	34.0
3	32.454857777859466	34.0	31.0	34.0	30.0	34.0
4	35.99218304870997	37.0	35.0	37.0	35.0	37.0
5	35.83084903486312	37.0	35.0	37.0	35.0	37.0
6	35.80258840195728	37.0	35.0	37.0	35.0	37.0
7	35.867459625142615	37.0	35.0	37.0	35.0	37.0
8	36.002426096656485	37.0	35.0	37.0	35.0	37.0
9	37.64769004777261	39.0	37.0	39.0	35.0	39.0
10	37.1621483679693	39.0	37.0	39.0	34.0	39.0
11	37.11663219328469	39.0	37.0	39.0	34.0	39.0
12	37.16239437077713	39.0	37.0	39.0	34.0	39.0
13	37.129408787389956	39.0	37.0	39.0	33.0	39.0
14	38.341326068238914	40.0	38.0	41.0	34.0	41.0
15	38.350128444569485	40.0	38.0	41.0	34.0	41.0
16	38.33707474385311	40.0	38.0	41.0	34.0	41.0
17	38.28168452543372	40.0	38.0	41.0	34.0	41.0
18	38.1967315557981	40.0	37.0	41.0	34.0	41.0
19	38.15221777186491	40.0	37.0	41.0	34.0	41.0
20	38.07201095984923	40.0	37.0	41.0	33.0	41.0
21	37.997243071981266	40.0	37.0	41.0	33.0	41.0
22	37.95552863034402	40.0	37.0	41.0	33.0	41.0
23	37.86420786388976	40.0	37.0	41.0	33.0	41.0
24	37.82468624038433	40.0	37.0	41.0	33.0	41.0
25	37.768255458364024	40.0	36.0	41.0	33.0	41.0
26	37.66590697983484	40.0	36.0	41.0	33.0	41.0
27	37.60024967871185	40.0	36.0	41.0	33.0	41.0
28	37.51620578841779	39.0	36.0	41.0	33.0	41.0
29	37.44746072791383	39.0	36.0	41.0	32.0	41.0
30	37.333762188802915	39.0	36.0	41.0	32.0	41.0
31	37.250802690196224	39.0	35.0	41.0	32.0	41.0
32	37.143138288923225	39.0	35.0	41.0	31.0	41.0
33	37.065367470228715	39.0	35.0	41.0	31.0	41.0
34	36.94050832097428	39.0	35.0	41.0	31.0	41.0
35	36.86704537903519	39.0	35.0	41.0	31.0	41.0
36	36.79779134720469	39.0	35.0	41.0	31.0	41.0
37	36.72053939650139	39.0	35.0	41.0	31.0	41.0
38	36.65256203441494	39.0	35.0	41.0	31.0	41.0
39	36.59154344140963	39.0	35.0	41.0	31.0	41.0
40	36.51931051350965	39.0	35.0	41.0	30.0	41.0
41	36.44883495049547	39.0	35.0	40.0	30.0	41.0
42	36.384735667155375	39.0	35.0	40.0	30.0	41.0
43	36.26140767330827	39.0	35.0	40.0	30.0	41.0
44	36.17310680339145	38.0	35.0	40.0	30.0	41.0
45	36.03626986225257	38.0	35.0	40.0	29.0	41.0
46	35.88658139511868	38.0	35.0	40.0	29.0	41.0
47	35.8099359685795	38.0	35.0	40.0	28.0	41.0
48	35.71267578950643	38.0	35.0	40.0	28.0	41.0
49	35.60391003083518	38.0	34.0	40.0	28.0	41.0
50	35.48716331900202	38.0	34.0	40.0	28.0	41.0
51	34.48606903064997	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	4.0
12	0.0
13	6.0
14	16.0
15	24.0
16	50.0
17	103.0
18	225.0
19	418.0
20	683.0
21	1037.0
22	1662.0
23	2247.0
24	3090.0
25	3788.0
26	4678.0
27	5655.0
28	7097.0
29	9234.0
30	12048.0
31	15589.0
32	20417.0
33	27366.0
34	40422.0
35	56340.0
36	66041.0
37	90339.0
38	141238.0
39	197400.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.105781207364814	32.7080526332904	20.084149925987084	27.102016233357695
2	21.2713255451295	32.39941807611666	20.166150861928802	26.163105516825034
3	20.48920627335436	33.83089993199578	21.05444720765606	24.625446586993803
4	19.451894433691642	33.64455987411443	19.727021711868503	27.176523980325427
5	19.245478284596974	35.340282677019516	17.39861927389585	28.01561976448766
6	20.537558549375166	37.79776589863836	19.246467951065235	22.41820760092124
7	83.90024727523614	7.536168774891879	4.689605250321995	3.8739786995499847
8	85.21141396475939	7.405956943853393	3.89193407690274	3.4906950144844755
9	76.4582381957532	12.512070396389696	6.823043394047016	4.206648013810089
10	31.517342491047053	42.49684367087087	13.447729351669496	12.538084486412588
11	20.876590005216954	30.18779628139894	31.997189347230137	16.938424366153974
12	25.045065169536933	26.71929807198834	30.438888802489434	17.79674795598529
13	22.43531469272977	28.401731068033914	29.084600931134762	20.07835330810155
14	18.705544535697978	30.686446800479	29.820064497977544	20.787944165845477
15	18.112875702133014	33.09388117498858	30.10904710671008	18.684196016168322
16	20.53685164475498	31.890446749581862	28.410496685324237	19.16220492033892
17	19.332144791031926	32.332403518122916	29.69169061895155	18.643761071893614
18	19.265130233038178	32.17462240689712	29.242240661436515	19.31800669862818
19	19.029306851743723	33.73603333196665	29.193040099871485	18.041619716418143
20	21.468976076933842	32.1337633198503	28.553432799526092	17.84382780368976
21	20.66508414285694	32.88831331143814	27.862362842831068	18.58423970287385
22	19.514667563964263	31.601181379001257	28.441459107688438	20.44269194934604
23	19.74879437417027	32.977100531733655	27.419416407821757	19.854688686274315
24	17.85259342098008	32.53175062101571	29.59357225766956	20.022083700334647
25	18.071875234162153	34.95586794456172	26.24482369091868	20.727433130357454
26	19.820333121733217	33.65799106189799	26.870717041632442	19.65095877473636
27	17.77681324569601	33.48465804902808	27.365974418535604	21.372554286740307
28	18.87788788209962	33.067301561269545	28.945764863729995	19.10904569290084
29	20.34994606317748	32.399135314268584	26.76199511104765	20.48892351150629
30	19.405097347835245	33.821144648237194	26.90987955759081	19.86387844633675
31	20.141126438374176	33.20302724834549	26.09043572186979	20.565410591410544
32	20.430109047106708	32.827943656874154	26.517547493386907	20.22439980263223
33	19.647424251635424	34.09641330733809	25.74984907586359	20.506313365162892
34	18.720106770873834	32.96522453411451	27.8360659909601	20.478602704051553
35	20.646846003656112	31.66466141389407	27.348160422106886	20.340332160342932
36	19.9607243793024	35.24442641052213	26.52617172975319	18.268677480422276
37	18.905174400438845	35.060772590197494	27.616077273157842	18.417975736205815
38	19.330024077171366	32.996469718326786	29.6156276818194	18.05787852268245
39	18.85682212441804	33.03690466260149	28.26148119138877	19.844792021591694
40	20.05742893134401	31.995634157065723	28.244798242352353	19.702138669237915
41	17.301066436310013	31.201497506747405	31.407630893994	20.089805162948586
42	19.10621807442009	32.02772762682222	27.772585956067292	21.093468342690393
43	18.139031173079943	32.3387656597046	29.480184756591534	20.04201841062393
44	19.368196926661476	32.35969003646214	26.70049440909136	21.57161862778503
45	19.702280050161953	32.70451811018946	26.990325303368117	20.602876536280466
46	16.938707128002047	34.033781557989506	28.344471793798746	20.683039520209697
47	18.485979960667827	32.19738473566716	28.40427592466659	20.91235937899843
48	19.121628595140173	30.72461964996911	29.836747447013963	20.317004307876754
49	18.611384840289038	31.898364081327962	28.681948059476127	20.80830301890687
50	17.54622095859094	32.08979385247466	28.795476941478192	21.568508247456204
51	17.868286703548236	31.43336222216881	28.873801973394936	21.824549100888014
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9625.0
1	7530.5
2	5436.0
3	3600.0
4	1764.0
5	1710.5
6	1657.0
7	1977.5
8	2298.0
9	2941.5
10	3585.0
11	4636.0
12	5687.0
13	5740.0
14	5793.0
15	5767.0
16	5741.0
17	5652.5
18	5564.0
19	5729.0
20	5894.0
21	5545.5
22	5197.0
23	6183.0
24	7169.0
25	8536.0
26	10590.5
27	11278.0
28	13638.5
29	15999.0
30	18525.5
31	21052.0
32	24269.5
33	27487.0
34	30315.5
35	33144.0
36	34274.0
37	35404.0
38	38174.5
39	40945.0
40	40578.5
41	40212.0
42	39641.0
43	39070.0
44	41737.0
45	44404.0
46	47854.5
47	51305.0
48	53161.0
49	55017.0
50	51297.0
51	47577.0
52	42983.0
53	38389.0
54	34502.0
55	30615.0
56	26780.0
57	22945.0
58	20307.5
59	17670.0
60	17122.5
61	16575.0
62	14764.5
63	12954.0
64	11062.0
65	9170.0
66	7734.0
67	6298.0
68	5524.0
69	4750.0
70	4088.0
71	3426.0
72	2957.0
73	2488.0
74	1903.0
75	1217.0
76	1116.0
77	923.5
78	731.0
79	530.5
80	330.0
81	244.0
82	158.0
83	128.5
84	99.0
85	67.0
86	35.0
87	27.5
88	20.0
89	12.0
90	4.0
91	4.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	2.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	707309.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.60405418340919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.66568251002327	20.33471491464501
2	9.665668616630324	5.336232804246824
3	3.8550175655054733	3.1924234126862174
4	2.2346456367362153	2.4674111694874163
5	1.4571388072092504	2.011146929347619
6	1.066015652509166	1.765581229933519
7	0.8418730327180161	1.6267376167489391
8	0.6660456735928785	1.4708448689986469
9	0.5356253500391825	1.3306888066040197
>10	4.932095803019807	29.018152888465416
>50	0.7264685294618918	13.956254683571384
>100	0.3475532384402612	14.660184224849251
>500	0.0035989240666890955	0.7398356247375472
>1k	0.0020565280381080546	0.9319970358050014
>5k	5.141320095270137E-4	1.1577937898731845
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8158	1.1533855782974627	No Hit
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT	2119	0.29958617803534243	TruSeq Adapter, Index 13 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1862	0.26325128055771946	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1523	0.21532314730902616	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1063	0.15028792225180226	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	900	0.12724283163369898	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	895	0.12653592701351177	No Hit
GTAGACGGGACTGAAGTCAATGTTCAAGAGTTAACAATGTGTTCCTTTTTA	819	0.11579097678666608	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	787	0.1112667872174679	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.34369702633502475	0.0
2	0.0	0.0	0.0	0.47306057182928535	0.0
3	0.0	0.0	0.0	0.6185415426638146	0.0
4	0.0	0.0	0.0	1.2915147410820447	0.0
5	0.0	0.0	0.0	1.3880779121996185	0.0
6	0.0	0.0	0.0	2.0476199228342917	0.0
7	0.0	0.0	0.0	2.58133291107564	0.0
8	0.0	0.0	0.0	3.13045642003707	0.0
9	0.0	0.0	0.0	3.8353817072877625	0.0
10	0.0	0.0	0.0	4.976608526118005	0.0
11	0.0	0.0	0.0	5.896998341601761	0.0
12	0.0	0.0	0.0	6.3615760579888	0.0
13	1.4138092403744333E-4	0.0	0.0	6.570395682792103	0.0
14	1.4138092403744333E-4	0.0	0.0	6.7850119254809425	0.0
15	1.4138092403744333E-4	0.0	0.0	6.968100222109432	0.0
16	1.4138092403744333E-4	0.0	0.0	7.342476908960582	0.0
17	1.4138092403744333E-4	0.0	0.0	7.827130716560937	0.0
18	1.4138092403744333E-4	0.0	0.0	8.397319983203946	0.0
19	1.4138092403744333E-4	0.0	0.0	8.701995874504636	0.0
20	1.4138092403744333E-4	0.0	0.0	9.00045100514768	0.0
21	1.4138092403744333E-4	0.0	0.0	9.359982694974898	0.0
22	1.4138092403744333E-4	0.0	0.0	9.769280470063297	0.0
23	1.4138092403744333E-4	0.0	0.0	10.159350439482603	0.0
24	1.4138092403744333E-4	0.0	0.0	10.45695728458142	0.0
25	1.4138092403744333E-4	0.0	0.0	10.725581040252562	0.0
26	1.4138092403744333E-4	0.0	0.0	10.977380465963249	0.0
27	1.4138092403744333E-4	0.0	0.0	11.253921553380488	0.0
28	1.4138092403744333E-4	0.0	0.0	11.529897117101578	0.0
29	1.4138092403744333E-4	0.0	0.0	11.846024863249301	0.0
30	1.4138092403744333E-4	0.0	0.0	12.20442550568422	0.0
31	1.4138092403744333E-4	0.0	0.0	12.513908348402184	0.0
32	1.4138092403744333E-4	0.0	0.0	12.82593604775282	0.0
33	1.4138092403744333E-4	0.0	0.0	13.142770698520732	0.0
34	1.4138092403744333E-4	0.0	0.0	13.444618971340672	0.0
35	1.4138092403744333E-4	0.0	0.0	13.772622715107541	0.0
36	1.4138092403744333E-4	0.0	0.0	14.095960888381175	0.0
37	1.4138092403744333E-4	0.0	0.0	14.411098968060635	0.0
38	1.4138092403744333E-4	0.0	0.0	14.747302805421675	0.0
39	1.4138092403744333E-4	0.0	0.0	15.090999831756701	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCC	65	0.0	45.000004	6
ACGTTAG	25	3.889278E-5	45.000004	1
ACGCACG	50	2.1827873E-11	45.000004	1
TAGGTAC	25	3.889278E-5	45.000004	23
TAACGCG	30	2.1643136E-6	45.000004	1
CGTAGCG	30	2.1643136E-6	45.000004	1
CTACGCG	30	2.1643136E-6	45.000004	1
ATGGTCG	25	3.889278E-5	45.000004	44
CGTAAGC	30	2.1643136E-6	45.000004	1
GTATACG	30	2.1643136E-6	45.000004	1
TTGCACG	30	2.1643136E-6	45.000004	1
GCGCTAC	30	2.1643136E-6	45.000004	9
AGATCGC	35	1.2110468E-7	45.0	30
CTCAACG	20	7.0314447E-4	45.0	1
ACGTTAC	20	7.0314447E-4	45.0	12
CAGCCGA	20	7.0314447E-4	45.0	42
TTTCGCG	35	1.2110468E-7	45.0	1
CGGTTAT	20	7.0314447E-4	45.0	33
TAGAACG	20	7.0314447E-4	45.0	1
ATTACGC	35	1.2110468E-7	45.0	1
>>END_MODULE