SRR3554218_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554218_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504034
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4128	0.8189923695623708	No Hit
CTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGCT	1146	0.22736561422443724	Illumina Single End Adapter 2 (95% over 21bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1075	0.2132792629068674	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	840	0.16665542403885453	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	760	0.15078347889229696	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	660	0.13094354745909997	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	653	0.1295547522587762	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	574	0.11388120642655059	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	517	0.10257244550962831	No Hit
AAACCAGGGAAAGAAGTGGGGTTTTTTTGTTTTTTGTTTTTTTAATTTTGT	516	0.10237404619529634	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	509	0.10098525099497256	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	40	6.8012014E-9	45.0	2
GACGTAC	25	3.8876773E-5	45.0	9
TAGCGGA	40	6.8012014E-9	45.0	3
AACGGCA	20	7.029514E-4	45.0	26
CTACGCG	20	7.029514E-4	45.0	1
CTTGACG	20	7.029514E-4	45.0	1
CGAATTG	20	7.029514E-4	45.0	1
GCGATAT	20	7.029514E-4	45.0	43
ACGTCCC	20	7.029514E-4	45.0	16
CCGACAG	25	3.8876773E-5	45.0	1
TAGTGCG	25	3.8876773E-5	45.0	1
TATCGCG	20	7.029514E-4	45.0	1
CAATACG	20	7.029514E-4	45.0	1
GCACTAT	20	7.029514E-4	45.0	11
ACGACGA	20	7.029514E-4	45.0	35
TCAACCG	25	3.8876773E-5	45.0	1
ACTATCG	30	2.1630676E-6	44.999996	1
GCGGTAC	30	2.1630676E-6	44.999996	25
TTAGCCG	30	2.1630676E-6	44.999996	1
TACACCG	30	2.1630676E-6	44.999996	1