##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554217_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	490647
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28397401798034	34.0	31.0	34.0	30.0	34.0
2	32.45314655954281	34.0	31.0	34.0	30.0	34.0
3	32.547095977352356	34.0	31.0	34.0	31.0	34.0
4	36.05856348861809	37.0	35.0	37.0	35.0	37.0
5	35.907428354804985	37.0	35.0	37.0	35.0	37.0
6	35.87895370806303	37.0	35.0	37.0	35.0	37.0
7	35.92114086094483	37.0	35.0	37.0	35.0	37.0
8	36.03976382205537	37.0	35.0	37.0	35.0	37.0
9	37.65417295937813	39.0	38.0	39.0	35.0	39.0
10	37.26133248547326	39.0	37.0	39.0	34.0	39.0
11	37.2333979419012	39.0	37.0	39.0	34.0	39.0
12	37.21051591062413	39.0	37.0	39.0	34.0	39.0
13	37.19543378437044	39.0	37.0	39.0	33.0	39.0
14	38.471359246056736	40.0	38.0	41.0	34.0	41.0
15	38.47191565422799	40.0	38.0	41.0	34.0	41.0
16	38.44349196061527	40.0	38.0	41.0	34.0	41.0
17	38.38962635051269	40.0	38.0	41.0	34.0	41.0
18	38.32475894074559	40.0	38.0	41.0	34.0	41.0
19	38.29741749159783	40.0	38.0	41.0	34.0	41.0
20	38.21288217394583	40.0	37.0	41.0	34.0	41.0
21	38.13055006960197	40.0	37.0	41.0	33.0	41.0
22	38.07755270082157	40.0	37.0	41.0	33.0	41.0
23	37.98375002802422	40.0	37.0	41.0	33.0	41.0
24	37.95806965088954	40.0	37.0	41.0	33.0	41.0
25	37.86347414740129	40.0	37.0	41.0	33.0	41.0
26	37.798792207024604	40.0	37.0	41.0	33.0	41.0
27	37.71941334605123	40.0	36.0	41.0	33.0	41.0
28	37.597617024051914	40.0	36.0	41.0	33.0	41.0
29	37.529270534620615	40.0	36.0	41.0	33.0	41.0
30	37.47949136548272	40.0	36.0	41.0	32.0	41.0
31	37.380874641035206	40.0	36.0	41.0	32.0	41.0
32	37.30708635740155	40.0	36.0	41.0	32.0	41.0
33	37.22866337713264	39.0	35.0	41.0	32.0	41.0
34	37.1195115836844	39.0	35.0	41.0	31.0	41.0
35	37.049777131012725	39.0	35.0	41.0	31.0	41.0
36	36.91121519136976	39.0	35.0	41.0	31.0	41.0
37	36.80408725621475	39.0	35.0	41.0	31.0	41.0
38	36.72409288144022	39.0	35.0	41.0	31.0	41.0
39	36.66127990184389	39.0	35.0	41.0	31.0	41.0
40	36.57644497979199	39.0	35.0	41.0	30.0	41.0
41	36.48088340497343	39.0	35.0	41.0	30.0	41.0
42	36.379472410918645	39.0	35.0	41.0	30.0	41.0
43	36.25231989597409	39.0	35.0	40.0	30.0	41.0
44	36.166276365696724	38.0	35.0	40.0	30.0	41.0
45	36.037173364965035	38.0	35.0	40.0	29.0	41.0
46	35.880847126345415	38.0	35.0	40.0	29.0	41.0
47	35.75884291557882	38.0	35.0	40.0	28.0	41.0
48	35.63920293000874	38.0	34.0	40.0	28.0	41.0
49	35.480168023038964	38.0	34.0	40.0	28.0	41.0
50	35.38721728656244	38.0	34.0	40.0	27.0	41.0
51	34.352705713068666	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	4.0
12	2.0
13	6.0
14	8.0
15	18.0
16	34.0
17	75.0
18	141.0
19	286.0
20	435.0
21	834.0
22	1100.0
23	1463.0
24	2086.0
25	2676.0
26	3219.0
27	3994.0
28	4953.0
29	6517.0
30	8216.0
31	10597.0
32	13500.0
33	18190.0
34	27220.0
35	36602.0
36	43544.0
37	61583.0
38	97563.0
39	145723.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.18273830269012	32.14367967194337	20.902400300012026	26.77118172535448
2	22.107747525206513	31.777836204032635	20.73588547367048	25.37853079709037
3	21.032636498337908	31.391815296944646	21.360774650614392	26.21477355410305
4	20.825970606158805	32.25149649340565	19.243774037138717	27.67875886329683
5	18.916654947446943	35.06125585196689	17.281263311505015	28.74082588908115
6	20.758916288084915	37.70857663452543	19.266804851553154	22.2657022258365
7	84.97616412614364	6.650810052848585	4.428030743080055	3.9449950779277163
8	86.0461798400887	6.063014753988102	4.238688914841016	3.6521164910821837
9	76.78983057065467	11.485242954710822	7.09389846468031	4.631028009954203
10	31.959840781661764	40.64266162842125	13.648712822049253	13.748784767867733
11	23.77371103868973	29.11196848243238	28.527637996359907	18.586682482517983
12	25.644506131699572	25.0173750170693	30.272069328865765	19.066049522365365
13	23.351819128619965	28.25942072406435	27.39973952760335	20.98902061971234
14	20.170713364190547	29.450908698106787	28.21315528271853	22.16522265498413
15	19.33019054432209	30.293265830627725	29.41911394546385	20.957429679586344
16	21.336724773615245	30.252095702205455	27.513263099539998	20.897916424639305
17	20.176623927181865	30.188913821953463	28.232925096861898	21.401537154002774
18	21.01572005943173	29.594392710033894	28.27715241303829	21.112734817496083
19	21.492233724041927	30.488925846891963	27.37895065087527	20.639889778190838
20	23.225862993149864	28.679478321481632	28.6438111310168	19.450847554351704
21	22.071876522224738	30.801574247880858	27.11480962891855	20.011739600975854
22	21.774310247489538	29.823681791593547	26.648894215189333	21.753113745727582
23	21.260295079762027	30.20399594820716	27.19878038589862	21.33692858613219
24	19.31673891820392	29.597653710304968	29.840190605465843	21.24541676602527
25	20.915444301096308	32.76388116099762	25.73499888922178	20.585675648684287
26	22.15523584165398	31.314162727989775	25.792677831516347	20.7379235988399
27	20.286478873813557	30.13001200455725	26.584285647318744	22.99922347431045
28	20.498240078916208	30.630575546166593	28.417171612177388	20.45401276273981
29	21.22422026426331	29.964923865834297	26.53577826828657	22.275077601615827
30	21.045884311939133	30.315685207491335	27.05244299873432	21.585987481835208
31	21.736401119338346	32.14897879738386	25.6135266291244	20.501093454153395
32	22.17948953117007	30.814618248965143	26.151387861334115	20.854504358530676
33	20.57283546011695	31.309678852617058	25.93921903119758	22.178266656068416
34	20.6698502181813	30.317315707626868	28.14447046450911	20.868363609682724
35	22.479501556108566	29.456411636064217	28.08638389718066	19.977702910646556
36	21.827301501894436	32.760212535692666	26.24228824389021	19.170197718522687
37	20.77746322712663	33.561195727274395	26.71370659557686	18.947634450022115
38	20.837180294590613	31.325983853972406	28.35949266988283	19.47734318155415
39	21.365666151021	29.959420927876863	27.705662115533165	20.969250805568972
40	23.452910137023157	29.732985221554397	27.69017236424558	19.12393227717687
41	20.555511396176882	29.096682543661736	29.89277423483686	20.45503182532452
42	21.698288178670204	29.508587640401345	27.520804162666845	21.2723200182616
43	20.963340242577658	30.092510501439936	27.955536261303955	20.988612994678455
44	21.768807309532107	30.409846590318494	26.265319058304648	21.556027041844747
45	21.01673912201644	30.776505308297004	27.035526559828142	21.17122900985841
46	19.585975253084193	31.746449076423577	27.47739209655822	21.19018357393401
47	20.781947102499355	30.40373221481024	27.95451719871924	20.859803483971167
48	20.88140761076701	29.599488022957445	29.217950991242176	20.301153375033373
49	20.62399240186937	31.37510267055541	27.317806895792696	20.683098031782524
50	20.262836621848294	29.666746153548274	28.683146946786593	21.387270277816842
51	20.442599261791063	28.761614765809227	28.38191204674644	22.41387392565327
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4802.0
1	3703.0
2	2604.0
3	1826.0
4	1048.0
5	1034.0
6	1020.0
7	1075.5
8	1131.0
9	1470.5
10	1810.0
11	1990.0
12	2170.0
13	2387.0
14	2604.0
15	2517.0
16	2430.0
17	2384.0
18	2338.0
19	2369.0
20	2400.0
21	2486.5
22	2573.0
23	3672.0
24	4771.0
25	4501.5
26	5815.0
27	7398.0
28	8922.5
29	10447.0
30	12148.5
31	13850.0
32	15211.5
33	16573.0
34	17902.0
35	19231.0
36	21494.5
37	23758.0
38	25106.5
39	26455.0
40	26696.5
41	26938.0
42	26864.5
43	26791.0
44	28165.5
45	29540.0
46	31633.0
47	33726.0
48	34727.0
49	35728.0
50	34365.5
51	33003.0
52	30733.0
53	28463.0
54	25371.5
55	22280.0
56	20706.0
57	19132.0
58	18365.0
59	17598.0
60	16625.5
61	15653.0
62	14232.5
63	12812.0
64	11759.5
65	10707.0
66	8914.5
67	7122.0
68	6404.0
69	5686.0
70	4885.0
71	4084.0
72	3420.5
73	2757.0
74	2423.5
75	1696.0
76	1302.0
77	1019.0
78	736.0
79	576.0
80	416.0
81	318.0
82	220.0
83	175.0
84	130.0
85	95.0
86	60.0
87	37.0
88	14.0
89	12.0
90	10.0
91	5.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	490647.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.88484718002573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.45536086084431	21.217468730191314
2	8.539431790064171	4.933203645205143
3	3.428125997782087	2.9706268667942646
4	2.0924646017178556	2.4176208100093466
5	1.4472867361663302	2.0902328099921337
6	1.1272765054100442	1.9536725753041546
7	0.9076971944174546	1.8353086322540395
8	0.7803259282760664	1.8031676151092717
9	0.6831395441847598	1.7759143202768297
>10	6.773045345494566	39.56116067057416
>50	0.6412717366469198	12.466453091414364
>100	0.11820350995588921	4.917600305165798
>500	0.00424683269302596	0.7687264435797364
>1k	0.00212341634651298	1.2888434841294305
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4013	0.8178996304879067	No Hit
CTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGCT	1160	0.23642251965262195	Illumina Single End Adapter 2 (95% over 21bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1131	0.2305119566613064	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	812	0.16549576375683536	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	690	0.14063063668992168	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	583	0.11882269737713673	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	575	0.1171921972416014	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	574	0.11698838472465947	No Hit
AAACCAGGGAAAGAAGTGGGGTTTTTTTGTTTTTTGTTTTTTTAATTTTGT	526	0.10720538391144753	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.26801345977861885	0.0
2	0.0	0.0	0.0	0.36421296777520296	0.0
3	0.0	0.0	0.0	0.46530397617839303	0.0
4	0.0	0.0	0.0	0.9363147028311597	0.0
5	0.0	0.0	0.0	1.0215083349128804	0.0
6	0.0	0.0	0.0	1.5314472523015528	0.0
7	0.0	0.0	0.0	1.9017745955850132	0.0
8	0.0	0.0	0.0	2.278420126893673	0.0
9	0.0	0.0	0.0	2.7961039199261384	0.0
10	0.0	0.0	0.0	3.681261681004877	0.0
11	0.0	0.0	0.0	4.472869496807277	0.0
12	0.0	0.0	0.0	4.860928529064684	0.0
13	0.0	0.0	0.0	5.031519605745067	0.0
14	0.0	0.0	0.0	5.220861433984107	0.0
15	0.0	0.0	0.0	5.397159261138864	0.0
16	0.0	0.0	0.0	5.707565724441401	0.0
17	0.0	0.0	0.0	6.097051444317401	0.0
18	0.0	0.0	0.0	6.582532859673044	0.0
19	0.0	0.0	0.0	6.793275002190985	0.0
20	0.0	0.0	0.0	7.036219522385748	0.0
21	0.0	0.0	0.0	7.283444105436291	0.0
22	0.0	0.0	0.0	7.569596879222741	0.0
23	0.0	0.0	0.0	7.8602335283819125	0.0
24	0.0	0.0	0.0	8.087076859738264	0.0
25	0.0	0.0	0.0	8.283755938587213	0.0
26	0.0	0.0	0.0	8.458423265606434	0.0
27	0.0	0.0	0.0	8.672630220912387	0.0
28	0.0	0.0	0.0	8.872570300032406	0.0
29	0.0	0.0	0.0	9.077401879559032	0.0
30	0.0	0.0	0.0	9.3592745904897	0.0
31	0.0	0.0	0.0	9.568590045389048	0.0
32	0.0	0.0	0.0	9.775459750085092	0.0
33	0.0	0.0	0.0	10.011067019669946	0.0
34	0.0	0.0	0.0	10.220790099603176	0.0
35	2.0381251694191548E-4	0.0	0.0	10.439277117764911	0.0
36	2.0381251694191548E-4	0.0	0.0	10.652872635520039	0.0
37	2.0381251694191548E-4	0.0	0.0	10.873601591368132	0.0
38	2.0381251694191548E-4	0.0	0.0	11.085770421504666	0.0
39	2.0381251694191548E-4	0.0	0.0	11.314855690547379	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTC	55	1.8189894E-12	45.000004	5
CGAATAT	85	0.0	45.000004	13
GTAACGG	75	0.0	45.000004	2
CCACACG	55	1.8189894E-12	45.000004	1
ATTTACG	25	3.8875252E-5	45.0	1
AACGTCG	25	3.8875252E-5	45.0	1
AGCGTAA	20	7.02933E-4	45.0	25
CTCGTAA	20	7.02933E-4	45.0	33
AGATACG	35	1.2100281E-7	45.0	1
CCTAGCG	20	7.02933E-4	45.0	1
TTTCGCG	20	7.02933E-4	45.0	1
ATTACGG	25	3.8875252E-5	45.0	2
ACCGACG	20	7.02933E-4	45.0	1
TACGGGT	35	1.2100281E-7	45.0	4
CCGTCAT	20	7.02933E-4	45.0	17
CGTTATG	20	7.02933E-4	45.0	40
ACGATAC	20	7.02933E-4	45.0	45
GTTCGGA	20	7.02933E-4	45.0	20
CGTAGCG	45	3.8380676E-10	45.0	1
GTCGTTT	35	1.2100281E-7	45.0	9
>>END_MODULE