##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554216_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1115941
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18988369456808	33.0	31.0	34.0	30.0	34.0
2	32.383160041615106	34.0	31.0	34.0	30.0	34.0
3	32.48039546893608	34.0	31.0	34.0	30.0	34.0
4	36.022939384788266	37.0	35.0	37.0	35.0	37.0
5	35.842840257683875	37.0	35.0	37.0	35.0	37.0
6	35.814440906822135	37.0	35.0	37.0	35.0	37.0
7	35.92286151328789	37.0	35.0	37.0	35.0	37.0
8	36.025906387524074	37.0	35.0	37.0	35.0	37.0
9	37.66501544436489	39.0	37.0	39.0	35.0	39.0
10	37.229946744496345	39.0	37.0	39.0	34.0	39.0
11	37.185291157865876	39.0	37.0	39.0	34.0	39.0
12	37.1114010507724	39.0	37.0	39.0	33.0	39.0
13	37.074902705429764	39.0	37.0	39.0	33.0	39.0
14	38.294152647855036	40.0	38.0	41.0	33.0	41.0
15	38.31268947014224	40.0	38.0	41.0	34.0	41.0
16	38.29451377805816	40.0	38.0	41.0	33.0	41.0
17	38.246806058743246	40.0	38.0	41.0	33.0	41.0
18	38.170373702552375	40.0	37.0	41.0	33.0	41.0
19	38.13005078225462	40.0	37.0	41.0	34.0	41.0
20	38.06200327795108	40.0	37.0	41.0	33.0	41.0
21	38.004770861541964	40.0	37.0	41.0	33.0	41.0
22	37.94654108057684	40.0	37.0	41.0	33.0	41.0
23	37.87435267635117	40.0	37.0	41.0	33.0	41.0
24	37.80277362333672	40.0	37.0	41.0	33.0	41.0
25	37.75482216353732	40.0	36.0	41.0	33.0	41.0
26	37.654443200850224	40.0	36.0	41.0	33.0	41.0
27	37.60291180268491	40.0	36.0	41.0	33.0	41.0
28	37.53466715534244	40.0	36.0	41.0	32.0	41.0
29	37.43476312815821	39.0	36.0	41.0	32.0	41.0
30	37.37397855262957	39.0	36.0	41.0	32.0	41.0
31	37.32749312015599	39.0	36.0	41.0	32.0	41.0
32	37.27192387411162	39.0	36.0	41.0	32.0	41.0
33	37.1708513263694	39.0	36.0	41.0	31.0	41.0
34	37.03621338404091	39.0	35.0	41.0	31.0	41.0
35	37.000138000127244	39.0	35.0	41.0	31.0	41.0
36	36.893802629350475	39.0	35.0	41.0	31.0	41.0
37	36.81016021456332	39.0	35.0	41.0	31.0	41.0
38	36.765943719246806	39.0	35.0	41.0	31.0	41.0
39	36.68595113899391	39.0	35.0	41.0	31.0	41.0
40	36.60131315186018	39.0	35.0	41.0	30.0	41.0
41	36.52621061507732	39.0	35.0	40.0	30.0	41.0
42	36.39932756301632	39.0	35.0	40.0	30.0	41.0
43	36.322952557527685	39.0	35.0	40.0	30.0	41.0
44	36.22800578166767	38.0	35.0	40.0	30.0	41.0
45	36.06493802091688	38.0	35.0	40.0	30.0	41.0
46	35.95927204036773	38.0	35.0	40.0	29.0	41.0
47	35.7929039259244	38.0	35.0	40.0	29.0	41.0
48	35.68800321880816	38.0	34.0	40.0	28.0	41.0
49	35.55848830717753	38.0	34.0	40.0	28.0	41.0
50	35.422784000229406	38.0	34.0	40.0	28.0	41.0
51	34.328782614851505	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	5.0
11	9.0
12	8.0
13	24.0
14	36.0
15	58.0
16	91.0
17	172.0
18	291.0
19	543.0
20	1001.0
21	1481.0
22	2212.0
23	3283.0
24	4239.0
25	5499.0
26	7278.0
27	9093.0
28	11639.0
29	14702.0
30	18978.0
31	24760.0
32	32555.0
33	43146.0
34	66926.0
35	83383.0
36	100934.0
37	145584.0
38	230759.0
39	307128.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.03448210971727	32.5090663395287	20.084126311337247	27.372325239416778
2	22.51391426607679	30.977712979449628	20.666683991357964	25.841688763115613
3	20.822068550219054	31.097791012248855	21.60383031002535	26.476310127506743
4	21.387779461458983	30.613984072634665	20.376973334611776	27.621263131294576
5	18.791405638828575	33.30256707119821	18.037960788249556	29.868066501723657
6	21.140992220914907	36.63052078918151	19.720307794050044	22.508179195853543
7	83.96743196996974	6.023167891492472	5.575205140773571	4.4341949977642185
8	85.14554084848571	5.681214329431395	4.916388948878122	4.256855873204766
9	76.25349368828638	10.113796338695327	8.371858368856417	5.260851604161869
10	33.73027785519127	35.78379143700249	15.56247149266852	14.923459215137719
11	25.103836134706047	27.252695258978743	28.714421282128715	18.92904732418649
12	26.33929571545449	23.555904837262904	30.968572711281332	19.136226736001277
13	24.087742989996784	25.052310113169064	29.43766740356345	21.4222794932707
14	20.238256323587002	26.444677630806645	30.21019928472921	23.106866760877143
15	19.47119068122777	28.1306986659689	30.758615374827166	21.639495277976163
16	21.92759294622207	27.979705020247486	28.567370497185784	21.525331536344662
17	21.89605005999421	27.612302084070755	29.072863171081625	21.418784684853414
18	22.149020423122728	27.53613318266826	28.606888715442842	21.70795767876617
19	21.45722757744361	29.338737442212448	28.02352454117198	21.18051043917196
20	22.881854865086954	28.240202663044013	28.649543300228235	20.228399171640795
21	22.841171710690798	29.33649718040649	27.256727730229464	20.565603378673245
22	22.780146979096568	27.797706151131646	27.702360608670173	21.719786261101618
23	21.74747589702323	28.213409131844784	27.893768577371027	22.145346393760963
24	20.492212401910137	27.819929548246726	29.8236197074935	21.86423834234964
25	21.02647003739445	29.397073859639534	26.767992214642174	22.808463888323846
26	21.68134336851142	28.722127782741204	27.61624494484923	21.980283903898147
27	20.528773474583335	27.914378985985817	27.495450028272106	24.061397511158745
28	20.658260606967573	28.22021952773489	29.13639699589853	21.98512286939901
29	21.246553357211535	27.744387920149897	27.58228257587095	23.426776146767615
30	21.413766498408073	27.635511196380453	28.99472283928989	21.955999465921586
31	21.496297743339476	28.8526902407923	27.529053955361437	22.12195806050678
32	22.7874950378201	26.906530004722473	27.32223298543561	22.983741972021818
33	21.6791927171777	27.012091141019106	28.616297815027856	22.69241832677534
34	20.675286596692835	26.470127004922304	30.358773447700194	22.49581295068467
35	21.23320139684804	26.69370513315668	30.183226532585504	21.889866937409774
36	21.753210967246474	29.07205667683148	28.366194987010964	20.808537368911082
37	20.559957918922237	29.130482704730802	28.652410835339857	21.657148541007096
38	20.692939859723765	27.12849514445656	30.23663437403949	21.941930621780184
39	20.312722626016967	26.995333982710555	28.56754971813026	24.12439367314222
40	21.138035075331043	27.757471049096683	28.718901805740625	22.38559206983165
41	19.460258203614707	26.471739993422595	31.588946010586582	22.47905579237612
42	20.282792728289397	27.57439685431398	29.51043110702089	22.63237931037573
43	19.807767614954557	27.110035387175486	30.397664392651585	22.684532605218376
44	21.792101912197868	26.288396967223175	28.268429961799058	23.6510711587799
45	21.035431084618274	26.761809092057735	29.42556998981129	22.777189833512704
46	19.998010647516313	27.918232236292063	29.891544445450073	22.192212670741554
47	20.726902228702055	27.231816018947235	30.168888857027387	21.87239289532332
48	20.188343290550307	26.370748991210107	31.309002895314357	22.13190482292523
49	20.385755160891122	27.44571621617989	29.558820762029537	22.609707860899455
50	19.59700378425024	26.110161737941347	30.16324339727638	24.129591080532034
51	20.60117873615182	24.914130764977717	30.83881674748038	23.64587375139008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7165.0
1	5726.5
2	4288.0
3	2905.5
4	1523.0
5	1572.5
6	1622.0
7	1775.0
8	1928.0
9	2309.5
10	2691.0
11	3215.5
12	3740.0
13	3642.0
14	3544.0
15	3650.5
16	3757.0
17	3577.5
18	3398.0
19	3747.5
20	4097.0
21	4588.0
22	5079.0
23	5879.0
24	6679.0
25	8384.0
26	12627.0
27	15165.0
28	19541.0
29	23917.0
30	25759.5
31	27602.0
32	29223.5
33	30845.0
34	37719.5
35	44594.0
36	47171.0
37	49748.0
38	52236.0
39	54724.0
40	56146.0
41	57568.0
42	60185.0
43	62802.0
44	66801.5
45	70801.0
46	76464.5
47	82128.0
48	85015.0
49	87902.0
50	85952.5
51	84003.0
52	75703.0
53	67403.0
54	60225.0
55	53047.0
56	50391.5
57	47736.0
58	44588.0
59	41440.0
60	39546.0
61	37652.0
62	35350.0
63	33048.0
64	29070.0
65	25092.0
66	21665.0
67	18238.0
68	15998.0
69	13758.0
70	11625.5
71	9493.0
72	8203.5
73	6914.0
74	5662.5
75	3586.5
76	2762.0
77	2270.0
78	1778.0
79	1334.0
80	890.0
81	664.5
82	439.0
83	321.5
84	204.0
85	152.0
86	100.0
87	72.5
88	45.0
89	36.0
90	27.0
91	18.5
92	10.0
93	12.0
94	14.0
95	18.5
96	23.0
97	15.0
98	7.0
99	9.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1115941.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.047354983330685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.43759113223906	19.38902360325459
2	9.38372592682323	4.888424805644967
3	3.435451700761203	2.684532899334426
4	1.830019086272304	1.9066862706562062
5	1.187839227330768	1.5470035008704874
6	0.8990240822639566	1.4050319645575415
7	0.7198737769867591	1.3125565468655596
8	0.5709713697783517	1.1897835163148236
9	0.4837613959384982	1.1340634326517485
>10	5.825966749523605	33.84555812999729
>50	0.8495440383468236	15.47627356919106
>100	0.37208571193133605	13.852504343232788
>500	0.002418384385811431	0.3841643618491796
>1k	0.0013819339347493893	0.4619151251749447
>5k	3.4548348368734733E-4	0.5224779304043891
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5806	0.5202784018151497	No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT	1897	0.16999106583591786	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1117	0.10009489749010028	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.792209444764553E-4	0.0	0.0	0.20664174898135296	0.0
2	1.792209444764553E-4	0.0	0.0	0.32707822366953093	0.0
3	1.792209444764553E-4	0.0	0.0	0.44160040718998583	0.0
4	1.792209444764553E-4	0.0	0.0	1.0028307948180055	0.0
5	1.792209444764553E-4	0.0	0.0	1.1215646705336573	0.0
6	1.792209444764553E-4	0.0	0.0	1.9666810342123822	0.0
7	1.792209444764553E-4	0.0	0.0	2.578989391016192	0.0
8	1.792209444764553E-4	0.0	0.0	3.101776886054012	0.0
9	1.792209444764553E-4	0.0	0.0	3.795720383066847	0.0
10	1.792209444764553E-4	0.0	0.0	4.980550047000692	0.0
11	1.792209444764553E-4	0.0	0.0	5.947536652923407	0.0
12	1.792209444764553E-4	0.0	0.0	6.448190361318385	0.0
13	1.792209444764553E-4	0.0	0.0	6.651068470465733	0.0
14	1.792209444764553E-4	0.0	0.0	6.8804712793955956	0.0
15	1.792209444764553E-4	0.0	0.0	7.088905237821713	0.0
16	1.792209444764553E-4	0.0	0.0	7.482115990003056	0.0
17	1.792209444764553E-4	0.0	0.0	7.939129398418017	0.0
18	1.792209444764553E-4	0.0	0.0	8.503316931629898	0.0
19	1.792209444764553E-4	0.0	0.0	8.785052256346885	0.0
20	1.792209444764553E-4	0.0	0.0	9.083007076538992	0.0
21	1.792209444764553E-4	0.0	0.0	9.426752848044833	0.0
22	1.792209444764553E-4	0.0	0.0	9.795320720360664	0.0
23	1.792209444764553E-4	0.0	0.0	10.143188573589464	0.0
24	1.792209444764553E-4	0.0	0.0	10.433884945530274	0.0
25	1.792209444764553E-4	0.0	0.0	10.698504670049761	0.0
26	1.792209444764553E-4	0.0	0.0	10.944575026815933	0.0
27	1.792209444764553E-4	0.0	0.0	11.213316833058379	0.0
28	1.792209444764553E-4	0.0	0.0	11.487435267635117	0.0
29	1.792209444764553E-4	0.0	0.0	11.766751109601673	0.0
30	1.792209444764553E-4	0.0	0.0	12.127164428943825	0.0
31	1.792209444764553E-4	0.0	0.0	12.40495689288233	0.0
32	1.792209444764553E-4	0.0	0.0	12.680061042653689	0.0
33	1.792209444764553E-4	0.0	0.0	12.97577560103984	0.0
34	1.792209444764553E-4	0.0	0.0	13.25285118120044	0.0
35	1.792209444764553E-4	0.0	0.0	13.549551454781211	0.0
36	1.792209444764553E-4	0.0	0.0	13.838186785860543	0.0
37	1.792209444764553E-4	0.0	0.0	14.119474058216339	0.0
38	1.792209444764553E-4	0.0	0.0	14.442430200162912	0.0
39	1.792209444764553E-4	0.0	0.0	14.748001910495269	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGT	35	1.2118835E-7	45.0	22
TCGCATA	20	7.0332E-4	45.0	24
ATAGGCG	20	7.0332E-4	45.0	1
ATTACGT	20	7.0332E-4	45.0	25
TACGGAC	25	3.8907332E-5	45.0	3
CGTAGCG	20	7.0332E-4	45.0	1
TACTCGA	20	7.0332E-4	45.0	35
CACGTAA	20	7.0332E-4	45.0	45
TAAGACG	50	2.1827873E-11	45.0	1
ATCGATA	20	7.0332E-4	45.0	38
TCTAACG	30	2.165445E-6	44.999996	1
CGATAGG	100	0.0	42.75	2
AATACCG	95	0.0	42.63158	1
TCATGCG	55	6.184564E-11	40.909092	1
CGTACAT	55	6.184564E-11	40.909092	35
TATTACG	50	1.0822987E-9	40.5	1
CATAACG	50	1.0822987E-9	40.5	1
TTACCGG	100	0.0	40.5	2
TCAACCG	50	1.0822987E-9	40.5	1
TATACGG	130	0.0	39.80769	2
>>END_MODULE