FastQCFastQC Report
Sat 18 Jun 2016
SRR3554215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088467
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55460.5095239451448689No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT19670.18071287416155016No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT253.8906684E-545.00000437
TAGAACG207.0331205E-445.0000041
GTCTACG207.0331205E-445.0000041
TCGATTA207.0331205E-445.00000432
TCGATCA453.8562575E-1045.00000417
CATAGCG253.8906684E-545.0000041
CGTACAC253.8906684E-545.0000041
TATAGCG302.165394E-645.0000041
CGTAACG600.045.0000041
CGTTTAG253.8906684E-545.00000423
TGCGTAG453.8562575E-1045.0000041
GCTACGA700.045.09
CTATGCG700.041.7857131
TATTACG650.041.5384641
TAGGCGG1150.041.0869562
CCATGCG556.184564E-1140.9090921
CGCGTAG501.0822987E-940.5000041
TCAAACG1000.040.5000041
TCGACGG451.9281288E-840.0000042
CGTCAAT451.9281288E-840.00000414