##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554214_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	587890
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25518209188794	34.0	31.0	34.0	30.0	34.0
2	32.430896936501725	34.0	31.0	34.0	30.0	34.0
3	32.53788804027965	34.0	31.0	34.0	31.0	34.0
4	36.05847012196159	37.0	35.0	37.0	35.0	37.0
5	35.89072445525523	37.0	35.0	37.0	35.0	37.0
6	35.85834935106908	37.0	35.0	37.0	35.0	37.0
7	35.85401690792495	37.0	35.0	37.0	35.0	37.0
8	35.961853407950464	37.0	35.0	37.0	35.0	37.0
9	37.59742468829203	39.0	37.0	39.0	35.0	39.0
10	37.23949038085356	39.0	37.0	39.0	34.0	39.0
11	37.180620524247736	39.0	37.0	39.0	34.0	39.0
12	37.06880198676623	39.0	37.0	39.0	33.0	39.0
13	37.030893534504756	39.0	37.0	39.0	33.0	39.0
14	38.29197979213799	40.0	38.0	41.0	33.0	41.0
15	38.30557757403596	40.0	38.0	41.0	33.0	41.0
16	38.29865621119597	40.0	38.0	41.0	34.0	41.0
17	38.26011158550069	40.0	37.0	41.0	34.0	41.0
18	38.168114783377845	40.0	37.0	41.0	33.0	41.0
19	38.14951266393373	40.0	37.0	41.0	34.0	41.0
20	38.04961642484138	40.0	37.0	41.0	33.0	41.0
21	38.021745564646444	40.0	37.0	41.0	33.0	41.0
22	37.93784891731446	40.0	37.0	41.0	33.0	41.0
23	37.86970861895933	40.0	37.0	41.0	33.0	41.0
24	37.784757352565954	40.0	36.0	41.0	33.0	41.0
25	37.71865655139567	40.0	36.0	41.0	33.0	41.0
26	37.597053870622055	40.0	36.0	41.0	33.0	41.0
27	37.52609331677695	40.0	36.0	41.0	33.0	41.0
28	37.43627039071935	40.0	36.0	41.0	32.0	41.0
29	37.34598139107656	39.0	36.0	41.0	32.0	41.0
30	37.29431186106245	39.0	36.0	41.0	32.0	41.0
31	37.24610216196908	39.0	35.0	41.0	32.0	41.0
32	37.17886339281158	39.0	35.0	41.0	32.0	41.0
33	37.078438143190056	39.0	35.0	41.0	31.0	41.0
34	36.944554253346716	39.0	35.0	41.0	31.0	41.0
35	36.91041861572743	39.0	35.0	41.0	31.0	41.0
36	36.77334365272415	39.0	35.0	41.0	31.0	41.0
37	36.71129973294324	39.0	35.0	41.0	31.0	41.0
38	36.662955654969466	39.0	35.0	41.0	31.0	41.0
39	36.57454115565837	39.0	35.0	41.0	30.0	41.0
40	36.46285699705727	39.0	35.0	41.0	30.0	41.0
41	36.34696626920002	39.0	35.0	40.0	30.0	41.0
42	36.26616884110973	39.0	35.0	40.0	30.0	41.0
43	36.18046913538247	39.0	35.0	40.0	30.0	41.0
44	36.074714657503954	38.0	35.0	40.0	29.0	41.0
45	35.964501862593345	38.0	35.0	40.0	29.0	41.0
46	35.85624351494327	38.0	35.0	40.0	29.0	41.0
47	35.70289680042185	38.0	34.0	40.0	28.0	41.0
48	35.59873105512936	38.0	34.0	40.0	28.0	41.0
49	35.46387759614894	38.0	34.0	40.0	27.0	41.0
50	35.34093793056524	38.0	34.0	40.0	27.0	41.0
51	34.27128884655293	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	3.0
11	9.0
12	10.0
13	18.0
14	29.0
15	38.0
16	54.0
17	135.0
18	225.0
19	369.0
20	620.0
21	1010.0
22	1480.0
23	2027.0
24	2659.0
25	3413.0
26	4147.0
27	5250.0
28	6488.0
29	7946.0
30	10140.0
31	12752.0
32	16885.0
33	22816.0
34	35646.0
35	44774.0
36	50375.0
37	71804.0
38	117313.0
39	169391.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.577846195716884	33.378523193114354	18.5670788752998	26.476551735868952
2	22.44756672166562	31.517971049005766	20.181496538468082	25.852965690860536
3	22.38684107571144	32.02367789892667	19.588698566058277	26.00078245930361
4	21.919406691728042	31.200564731497387	19.023626868972087	27.85640170780248
5	19.68548537991801	34.22136794298253	17.26292333599823	28.830223341101224
6	20.996274813315416	38.655700896426204	18.630866318528977	21.717157971729407
7	82.42596404089201	7.9455340284747145	5.314939869703516	4.313562060929766
8	83.87606525030192	7.1018387793634865	5.264080014968787	3.7580159553657997
9	74.54268655700896	12.159417578118356	8.161050536665023	5.136845328207658
10	34.60375240266036	35.10962935242988	15.51038459575771	14.77623364915205
11	26.77235537260372	30.68465189065982	25.39454659885353	17.148446137882935
12	29.768834305737467	25.102825358485433	27.788191668509416	17.340148667267684
13	24.725373794417322	27.091292588749592	26.16577931245641	22.01755430437667
14	20.648590722754257	29.26074605793601	26.63508479477453	23.455578424535204
15	20.457738692612566	31.19699263467656	28.1928592083553	20.15240946435558
16	21.89474221367943	28.900814778274846	28.413138512306723	20.791304495739
17	21.024511388184862	29.558420793005496	28.770007994692886	20.647059824116756
18	21.671060912755788	30.189491231352804	26.451036758577285	21.688411097314123
19	22.76990593478372	30.094065216281958	26.18159859837725	20.954430250557078
20	24.737791083365938	28.432189695351173	27.40053411352464	19.429485107758254
21	24.025753117079727	30.063617343380567	25.57179064110633	20.33883889843338
22	22.284100767150317	29.755226317848578	26.62130670703703	21.339366207964076
23	22.865672149551784	29.47098947081937	27.43625508173298	20.227083297895867
24	21.390396162547415	28.181972818044194	29.24118457534573	21.186446444062664
25	21.727363962646074	30.98470802360986	25.18651448400211	22.101413529741958
26	22.078450050179455	30.349044889350047	26.312745581656433	21.259759478814065
27	21.186956743608498	30.988960519825138	26.11304835938696	21.711034377179406
28	21.62190205650717	30.515402541291735	28.006429774277503	19.85626562792359
29	21.872629233359984	29.88739390021943	25.436561261460476	22.80341560496011
30	21.805269693309974	31.05989215669598	25.810610828556364	21.324227321437682
31	21.213662419840446	31.38971576315297	24.91333412713263	22.483287689873958
32	22.376635084794774	30.048648556702783	25.822007518413308	21.752708840089134
33	21.571552501318276	31.63431934545578	25.68014424467162	21.11398390855432
34	20.482062970963955	30.756774226470938	27.1433431424246	21.6178196601405
35	22.92911939308374	29.148990457398494	26.905033254520404	21.016856894997364
36	21.161441766316827	33.559679531885216	24.97525047202708	20.303628229770876
37	20.598751467111196	33.005324125261524	26.417186888703668	19.97873751892361
38	20.914116586436236	29.961727534062494	28.926329755566517	20.19782612393475
39	21.32201602340574	30.172651346340302	27.08142679753015	21.423905832723808
40	22.628893160285088	29.996768102876388	27.753831499089966	19.62050723774856
41	19.04693054823181	29.448196091105476	30.61916344894453	20.885709911718177
42	20.16737825103336	29.91937267175832	27.35596795318852	22.5572811240198
43	20.896086002483457	30.131317083127797	26.88019867662318	22.09239823776557
44	21.216043817721005	29.78669478984164	26.466856044498122	22.53040534793924
45	20.54891221146813	30.591777373318134	26.79055605640511	22.068754358808622
46	18.530337307999794	32.04187858272806	27.34848355984963	22.079300549422513
47	20.464202486859786	30.178774940890303	28.574223068941468	20.782799503308443
48	20.967527938900137	28.170235928490023	28.622531425947027	22.23970470666281
49	18.758271105138714	31.286465155046013	27.42332749323853	22.531936246576738
50	19.02005477215125	29.06479103233598	29.48884995492354	22.426304240589225
51	19.906274983415265	29.002364387895696	27.92103965027471	23.17032097841433
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5686.0
1	4507.0
2	3328.0
3	2205.5
4	1083.0
5	1104.5
6	1126.0
7	1189.5
8	1253.0
9	1357.5
10	1462.0
11	1666.0
12	1870.0
13	1886.0
14	1902.0
15	1981.0
16	2060.0
17	2175.5
18	2291.0
19	2257.5
20	2224.0
21	2418.0
22	2612.0
23	4566.5
24	6521.0
25	6496.5
26	6887.0
27	7302.0
28	10147.5
29	12993.0
30	14283.0
31	15573.0
32	18755.5
33	21938.0
34	24140.5
35	26343.0
36	27154.5
37	27966.0
38	28467.5
39	28969.0
40	30319.5
41	31670.0
42	32174.0
43	32678.0
44	34337.0
45	35996.0
46	36703.5
47	37411.0
48	37503.5
49	37596.0
50	36458.0
51	35320.0
52	32563.5
53	29807.0
54	27818.5
55	25830.0
56	24680.0
57	23530.0
58	23476.5
59	23423.0
60	23562.0
61	23701.0
62	22180.0
63	20659.0
64	18126.5
65	15594.0
66	12949.0
67	10304.0
68	8744.0
69	7184.0
70	6263.5
71	5343.0
72	4721.5
73	4100.0
74	3508.5
75	2344.0
76	1771.0
77	1373.0
78	975.0
79	802.0
80	629.0
81	441.0
82	253.0
83	174.0
84	95.0
85	75.5
86	56.0
87	43.0
88	30.0
89	23.0
90	16.0
91	19.0
92	22.0
93	13.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	587890.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.132239725997746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.9118743415652	18.827031842760363
2	7.700226461198205	3.8704787473460915
3	2.901798750163652	2.187861054771406
4	1.7697089017437777	1.7790699345542722
5	1.252402150651689	1.5737835541766696
6	0.990645959042313	1.493829103574541
7	0.7658613419258714	1.347346758950873
8	0.6452363702403063	1.2972988109449612
9	0.6456311182607023	1.4603540434822773
>10	7.169271461075074	37.675955158255306
>50	0.9909372742650656	17.29655827401356
>100	0.24824440981931978	8.839122268440763
>500	0.006801216707378624	1.1676215233547116
>1k	0.0013602433414757248	1.1836889253742318
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4642	0.7896034972528875	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2283	0.3883379543792206	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	936	0.1592134583000221	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	908	0.15445066253891032	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	816	0.13880147646668595	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	768	0.13063668373335147	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	697	0.1185595944819609	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	590	0.1003589106805695	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7009984861113473E-4	0.0	0.0	0.10648250523057035	0.0
2	3.4019969722226946E-4	0.0	0.0	0.16091445678613345	0.0
3	3.4019969722226946E-4	0.0	0.0	0.22215040228614197	0.0
4	3.4019969722226946E-4	0.0	0.0	0.5094490465903485	0.0
5	3.4019969722226946E-4	0.0	0.0	0.5671128952695232	0.0
6	3.4019969722226946E-4	0.0	0.0	0.9023796968820698	0.0
7	3.4019969722226946E-4	0.0	0.0	1.1401792852404362	0.0
8	3.4019969722226946E-4	0.0	0.0	1.3677728826821345	0.0
9	3.4019969722226946E-4	0.0	0.0	1.6737825103335657	0.0
10	3.4019969722226946E-4	0.0	0.0	2.2250761196822535	0.0
11	3.4019969722226946E-4	0.0	0.0	2.676351018047594	0.0
12	3.4019969722226946E-4	0.0	0.0	2.8779193386517887	0.0
13	3.4019969722226946E-4	0.0	0.0	2.9733453537226353	0.0
14	3.4019969722226946E-4	0.0	0.0	3.083059756076817	0.0
15	3.4019969722226946E-4	0.0	0.0	3.1823980676657198	0.0
16	3.4019969722226946E-4	0.0	0.0	3.3637245062851893	0.0
17	3.4019969722226946E-4	0.0	0.0	3.5722669206824404	0.0
18	3.4019969722226946E-4	0.0	0.0	3.8289475922366427	0.0
19	3.4019969722226946E-4	0.0	0.0	3.953120481722771	0.0
20	3.4019969722226946E-4	0.0	0.0	4.108081443807515	0.0
21	3.4019969722226946E-4	0.0	0.0	4.281413189542262	0.0
22	3.4019969722226946E-4	0.0	0.0	4.473455918624232	0.0
23	3.4019969722226946E-4	0.0	0.0	4.653081358757591	0.0
24	3.4019969722226946E-4	0.0	0.0	4.7980064297742775	0.0
25	3.4019969722226946E-4	0.0	0.0	4.934086308663185	0.0
26	3.4019969722226946E-4	0.0	0.0	5.05162530405348	0.0
27	3.4019969722226946E-4	0.0	0.0	5.195359676129888	0.0
28	3.4019969722226946E-4	0.0	0.0	5.334671452142407	0.0
29	3.4019969722226946E-4	0.0	0.0	5.482148020888261	0.0
30	3.4019969722226946E-4	0.0	0.0	5.658371464049397	0.0
31	3.4019969722226946E-4	0.0	0.0	5.813162326285529	0.0
32	3.4019969722226946E-4	0.0	0.0	5.981901376107775	0.0
33	3.4019969722226946E-4	0.0	0.0	6.140944734559186	0.0
34	3.4019969722226946E-4	0.0	0.0	6.2918233002772626	0.0
35	3.4019969722226946E-4	0.0	0.0	6.464644746466176	0.0
36	3.4019969722226946E-4	0.0	0.0	6.6466515844800895	0.0
37	3.4019969722226946E-4	0.0	0.0	6.814370035210668	0.0
38	3.4019969722226946E-4	0.0	0.0	6.996717072921805	0.0
39	3.4019969722226946E-4	0.0	0.0	7.183997006242665	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	30	2.1636843E-6	45.000004	1
TAGCACG	45	3.8380676E-10	45.000004	1
CGTACGG	45	3.8380676E-10	45.000004	2
AATTTCG	25	3.8884733E-5	45.0	26
ACGTTAC	20	7.030471E-4	45.0	35
ACCGCGA	20	7.030471E-4	45.0	12
ATATGCG	20	7.030471E-4	45.0	1
TACGGGT	50	2.1827873E-11	45.0	4
CGCGACT	20	7.030471E-4	45.0	14
TATGCGG	50	2.1827873E-11	45.0	2
ATCGTAC	25	3.8884733E-5	45.0	8
CATCGTA	25	3.8884733E-5	45.0	7
TTTGCGC	20	7.030471E-4	45.0	13
CCGCGAC	20	7.030471E-4	45.0	13
CGATACG	25	3.8884733E-5	45.0	1
GCGTATG	20	7.030471E-4	45.0	1
TACACCG	25	3.8884733E-5	45.0	1
TCAAACG	50	2.1827873E-11	45.0	1
CGAATAT	165	0.0	43.636368	13
ATACACG	85	0.0	42.352943	1
>>END_MODULE