FastQCFastQC Report
Sat 18 Jun 2016
SRR3554212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461088
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79171.7170258171975847No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14520.3149073495731834No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13600.29495454229995144No Hit
CTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGCT10310.22360156846415435No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7590.16461066000416405No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG207.0288865E-445.024
TAATACG502.1827873E-1145.01
TAAAGCG253.8871593E-545.01
CGAAACG351.2098099E-745.01
CCGATAG207.0288865E-445.01
ATCAGCG351.2098099E-745.01
CGATTTA207.0288865E-445.040
TAACGTC453.8380676E-1045.032
CCTGTCG253.8871593E-545.031
CATTACG207.0288865E-445.011
ATATCCG406.7993824E-945.01
TACGCGG406.7993824E-945.02
GCACACG406.7993824E-945.01
TCGTGAG207.0288865E-445.02
CTACGTC253.8871593E-545.010
CTAAACG253.8871593E-545.01
ATGACCG207.0288865E-445.01
CGTACGA207.0288865E-445.041
GTATCAC207.0288865E-445.09
TGCTCGT253.8871593E-545.037