##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554212_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	461088
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22453631410924	33.0	31.0	34.0	30.0	34.0
2	32.412478745922684	34.0	31.0	34.0	30.0	34.0
3	32.5026524220973	34.0	31.0	34.0	30.0	34.0
4	36.05299855992782	37.0	35.0	37.0	35.0	37.0
5	35.86700369560691	37.0	35.0	37.0	35.0	37.0
6	35.85363965230064	37.0	35.0	37.0	35.0	37.0
7	35.86076193698383	37.0	35.0	37.0	35.0	37.0
8	35.982408997848566	37.0	35.0	37.0	35.0	37.0
9	37.67815904989937	39.0	38.0	39.0	35.0	39.0
10	37.28157748629329	39.0	37.0	39.0	34.0	39.0
11	37.20263810812686	39.0	37.0	39.0	34.0	39.0
12	37.1167477791658	39.0	37.0	39.0	33.0	39.0
13	37.08531777014366	39.0	37.0	39.0	33.0	39.0
14	38.31020759594698	40.0	38.0	41.0	33.0	41.0
15	38.331641682281905	40.0	38.0	41.0	33.0	41.0
16	38.33147251717676	40.0	38.0	41.0	34.0	41.0
17	38.27131263446457	40.0	38.0	41.0	34.0	41.0
18	38.1497826879034	40.0	37.0	41.0	33.0	41.0
19	38.095040426122566	40.0	37.0	41.0	34.0	41.0
20	37.99266517454369	40.0	37.0	41.0	33.0	41.0
21	37.91249826497328	40.0	37.0	41.0	33.0	41.0
22	37.87601499063086	40.0	36.0	41.0	33.0	41.0
23	37.78844819210216	40.0	36.0	41.0	33.0	41.0
24	37.71677206953987	40.0	36.0	41.0	33.0	41.0
25	37.625479301131236	40.0	36.0	41.0	33.0	41.0
26	37.503493910056214	39.0	36.0	41.0	33.0	41.0
27	37.42602496703449	39.0	36.0	41.0	32.0	41.0
28	37.353730741203414	39.0	35.0	41.0	32.0	41.0
29	37.25934095010063	39.0	35.0	41.0	32.0	41.0
30	37.17461742660837	39.0	35.0	41.0	32.0	41.0
31	37.13302449857728	39.0	35.0	41.0	32.0	41.0
32	37.016040322020956	39.0	35.0	41.0	31.0	41.0
33	36.92467381497675	39.0	35.0	41.0	31.0	41.0
34	36.751112585883824	39.0	35.0	41.0	31.0	41.0
35	36.72155423693525	39.0	35.0	41.0	31.0	41.0
36	36.580893452009164	39.0	35.0	41.0	30.0	41.0
37	36.50654972586578	39.0	35.0	41.0	30.0	41.0
38	36.44689083211882	39.0	35.0	40.0	30.0	41.0
39	36.35145785620099	39.0	35.0	40.0	30.0	41.0
40	36.248451488652925	39.0	35.0	40.0	30.0	41.0
41	36.15227462002915	38.0	35.0	40.0	30.0	41.0
42	36.02634204316747	38.0	35.0	40.0	29.0	41.0
43	35.92721129155389	38.0	35.0	40.0	29.0	41.0
44	35.834957752099385	38.0	35.0	40.0	29.0	41.0
45	35.70948929488514	38.0	35.0	40.0	29.0	41.0
46	35.61547036574363	38.0	34.0	40.0	28.0	41.0
47	35.45634239017281	38.0	34.0	40.0	28.0	41.0
48	35.34015198834062	38.0	34.0	40.0	27.0	41.0
49	35.168822437365534	37.0	34.0	40.0	27.0	41.0
50	35.03948053300021	37.0	34.0	40.0	26.0	41.0
51	33.957834513151504	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	1.0
12	2.0
13	9.0
14	14.0
15	14.0
16	51.0
17	101.0
18	180.0
19	320.0
20	575.0
21	839.0
22	1210.0
23	1695.0
24	2105.0
25	2811.0
26	3358.0
27	4149.0
28	5144.0
29	6371.0
30	8170.0
31	10317.0
32	13606.0
33	18840.0
34	28627.0
35	38821.0
36	41378.0
37	58407.0
38	90782.0
39	123151.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.412424526337706	33.27282427649386	19.616428968006108	24.69832222916233
2	23.953128253175098	31.881549725865778	19.868441599000626	24.296880421958498
3	21.74140294260532	32.79937886043445	20.31282531750989	25.146392879450342
4	20.707543896176002	34.53397182316608	18.562400235963633	26.196084044694288
5	18.790339371226317	34.668436393920466	17.728286140606567	28.812938094246654
6	19.799691165243946	39.22006211395656	19.176599694635296	21.803647026164203
7	82.48685717260045	8.448278853494344	4.7179714067596645	4.3468925671455345
8	83.54283780970226	8.010618363522799	4.618641127073357	3.8279026997015757
9	75.73218127559164	11.561567423138316	7.950759941703102	4.755491359566937
10	34.07505725588174	35.07269761954334	14.88154105073218	15.970704073842738
11	24.339388576584078	29.895594767159416	25.983543271566383	19.781473384690123
12	25.97096432785065	27.094177250329654	28.590204039142204	18.344654382677493
13	23.59961655909501	26.4127455062808	28.60733742799639	21.380300506627805
14	19.583680338677215	29.38853320841141	30.264938580054135	20.762847872857243
15	20.033269137344714	29.97779165799153	30.09425532653203	19.894683878131723
16	21.86784301478243	29.285299118606428	27.826792282601154	21.020065584009995
17	21.676990075647165	27.903133458255258	28.869760219307377	21.550116246790203
18	22.241741272815602	28.85804878895135	28.796238462072317	20.103971476160734
19	21.86155354292456	30.616932125754737	27.61229960441391	19.909214726906793
20	22.981296411964745	29.756792629606494	27.7246425844958	19.53726837393296
21	23.98652751752377	30.146306128114375	27.272017489069334	18.595148865292526
22	22.883484280657925	28.319973627593864	26.96426712471372	21.832274967034493
23	22.274923658824346	29.642497744465263	26.73199042265251	21.35058817405788
24	20.117417933236172	29.837037615379273	28.472872857242002	21.57267159414255
25	21.269475674925395	30.681561871052814	26.349850787702128	21.699111666319663
26	21.706268651537233	28.136928308695953	28.23322229162329	21.92358074814352
27	19.6229353182039	30.4861544867791	26.712905128738985	23.178005066278022
28	19.650695745714483	31.231999097786105	28.21630578110903	20.90099937539038
29	21.56855090568395	30.302458532861404	27.078345131515025	21.05064542993962
30	21.291380387257963	29.45641612880838	27.752186133666456	21.500017350267196
31	22.216800263724064	30.53278332986328	25.333558886806856	21.916857519605802
32	22.029851134707474	30.31872440835589	26.937157332222917	20.71426712471372
33	21.97714969810535	29.95914012075786	26.090681171490044	21.97302900964675
34	20.42538517593171	30.98215525019085	27.642879450343532	20.949580123533902
35	21.158000208203205	30.687634464570756	27.464171698244154	20.690193628981888
36	21.18315809563467	32.04377472413075	26.480411548337845	20.292655631896732
37	21.637301339440626	31.706745783885072	26.75823270178361	19.897720174890694
38	21.817310361579565	30.531265181483796	27.928291345686723	19.723133111249915
39	20.879962176417514	30.34041224234853	26.708784440280382	22.070841140953572
40	21.322393989867443	30.54601290859879	27.020872371434518	21.110720730099246
41	20.49760566312721	29.837037615379273	28.519284821986258	21.146071899507252
42	21.78369421889097	29.755708237906862	27.148613713651194	21.311983829550975
43	20.265112082726073	30.33021896037199	27.959738704976054	21.44493025192588
44	20.896878686931778	28.475041640641262	27.677579984731764	22.950499687695192
45	21.566165243944756	29.0627819418419	28.72358421819696	20.647468596016378
46	20.68563918384343	29.910776250954264	28.565696786730516	20.83788777847179
47	20.94112186827677	30.26428794503435	27.639626275244638	21.154963911444234
48	20.606261711430356	30.00663647720175	28.642688597404398	20.744413213963494
49	21.184893122354083	30.075820667638283	27.735052744812272	21.004233465195362
50	19.937192032757302	28.756983482545635	28.923112290929282	22.382712193767784
51	20.34514019015893	28.705149559303212	28.985573252828097	21.964136997709765
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9237.0
1	6831.5
2	4426.0
3	2854.0
4	1282.0
5	1090.5
6	899.0
7	978.5
8	1058.0
9	1246.5
10	1435.0
11	1739.0
12	2043.0
13	2299.5
14	2556.0
15	2402.5
16	2249.0
17	2191.0
18	2133.0
19	2286.5
20	2440.0
21	2625.0
22	2810.0
23	2863.5
24	2917.0
25	3471.5
26	4850.5
27	5675.0
28	7028.5
29	8382.0
30	8952.0
31	9522.0
32	11915.0
33	14308.0
34	15071.5
35	15835.0
36	17622.0
37	19409.0
38	20181.5
39	20954.0
40	22286.5
41	23619.0
42	23506.5
43	23394.0
44	25633.0
45	27872.0
46	29052.0
47	30232.0
48	32614.0
49	34996.0
50	33710.0
51	32424.0
52	29969.0
53	27514.0
54	24945.0
55	22376.0
56	20939.5
57	19503.0
58	19282.0
59	19061.0
60	17902.0
61	16743.0
62	15267.5
63	13792.0
64	12475.0
65	11158.0
66	9425.5
67	7693.0
68	6719.0
69	5745.0
70	4984.0
71	4223.0
72	3441.5
73	2660.0
74	2282.0
75	1632.0
76	1360.0
77	1010.0
78	660.0
79	456.0
80	252.0
81	197.5
82	143.0
83	111.0
84	79.0
85	60.5
86	42.0
87	33.0
88	24.0
89	19.0
90	14.0
91	10.0
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	461088.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.54406818517342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.52846015825871	19.548482043888878
2	8.208221538140045	4.193427412985167
3	3.070894060526209	2.3532938181457665
4	1.60776995416208	1.642759413407573
5	1.0749564954920596	1.3729381008472117
6	0.8143498039499678	1.2481084151208353
7	0.6330749516692521	1.1319916812235322
8	0.539471167664963	1.1024230632615148
9	0.4135372517519495	0.9507081380274923
>10	5.878013168828413	34.38284620567973
>50	0.850899412271146	15.02440043441808
>100	0.3760975402238465	14.327562465076834
>500	8.508994122711458E-4	0.16497193347785608
>1k	0.0025526982368134377	0.8352926750400539
>5k	8.508994122711458E-4	1.7207941993994553
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7917	1.7170258171975847	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1452	0.3149073495731834	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1360	0.29495454229995144	No Hit
CTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGCT	1031	0.22360156846415435	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	759	0.16461066000416405	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2704472898882643	0.0
2	0.0	0.0	0.0	0.3552467207995003	0.0
3	0.0	0.0	0.0	0.45522763550558676	0.0
4	0.0	0.0	0.0	0.9199979179679367	0.0
5	0.0	0.0	0.0	0.9969897286418211	0.0
6	0.0	0.0	0.0	1.5253053647026165	0.0
7	0.0	0.0	0.0	1.920674578388507	0.0
8	0.0	0.0	0.0	2.293054688042196	0.0
9	0.0	0.0	0.0	2.761945658963148	0.0
10	0.0	0.0	0.0	3.540538899299049	0.0
11	0.0	0.0	0.0	4.2091748212922475	0.0
12	0.0	0.0	0.0	4.551842598376015	0.0
13	0.0	0.0	0.0	4.6986692345062115	0.0
14	0.0	0.0	0.0	4.86241238115067	0.0
15	0.0	0.0	0.0	5.000130127003956	0.0
16	0.0	0.0	0.0	5.279252550489278	0.0
17	0.0	0.0	0.0	5.613245193975987	0.0
18	0.0	0.0	0.0	6.0563276424456935	0.0
19	0.0	0.0	0.0	6.2625789437157335	0.0
20	0.0	0.0	0.0	6.4805416753418	0.0
21	0.0	0.0	0.0	6.72387917273926	0.0
22	0.0	0.0	0.0	6.987169477409952	0.0
23	0.0	0.0	0.0	7.257616767298217	0.0
24	0.0	0.0	0.0	7.465386216947741	0.0
25	0.0	0.0	0.0	7.660142966201679	0.0
26	0.0	0.0	0.0	7.844489555139149	0.0
27	0.0	0.0	0.0	8.037511277673676	0.0
28	0.0	0.0	0.0	8.218387813172322	0.0
29	0.0	0.0	0.0	8.409891387327365	0.0
30	0.0	0.0	0.0	8.667759733499896	0.0
31	0.0	0.0	0.0	8.880517384967728	0.0
32	0.0	0.0	0.0	9.086334929557916	0.0
33	0.0	0.0	0.0	9.298875702685821	0.0
34	0.0	0.0	0.0	9.502958220556597	0.0
35	0.0	0.0	0.0	9.729162329099868	0.0
36	0.0	0.0	0.0	9.93649802206954	0.0
37	0.0	0.0	0.0	10.140146783260462	0.0
38	0.0	0.0	0.0	10.36353147338469	0.0
39	0.0	0.0	0.0	10.586916163508919	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	20	7.0288865E-4	45.0	24
TAATACG	50	2.1827873E-11	45.0	1
TAAAGCG	25	3.8871593E-5	45.0	1
CGAAACG	35	1.2098099E-7	45.0	1
CCGATAG	20	7.0288865E-4	45.0	1
ATCAGCG	35	1.2098099E-7	45.0	1
CGATTTA	20	7.0288865E-4	45.0	40
TAACGTC	45	3.8380676E-10	45.0	32
CCTGTCG	25	3.8871593E-5	45.0	31
CATTACG	20	7.0288865E-4	45.0	11
ATATCCG	40	6.7993824E-9	45.0	1
TACGCGG	40	6.7993824E-9	45.0	2
GCACACG	40	6.7993824E-9	45.0	1
TCGTGAG	20	7.0288865E-4	45.0	2
CTACGTC	25	3.8871593E-5	45.0	10
CTAAACG	25	3.8871593E-5	45.0	1
ATGACCG	20	7.0288865E-4	45.0	1
CGTACGA	20	7.0288865E-4	45.0	41
GTATCAC	20	7.0288865E-4	45.0	9
TGCTCGT	25	3.8871593E-5	45.0	37
>>END_MODULE