##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554207_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	234786
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21909739081546	33.0	31.0	34.0	30.0	34.0
2	32.395969095261215	34.0	31.0	34.0	30.0	34.0
3	32.48245636451918	34.0	31.0	34.0	30.0	34.0
4	36.022194679410184	37.0	35.0	37.0	35.0	37.0
5	35.8551063521675	37.0	35.0	37.0	35.0	37.0
6	35.838900104776265	37.0	35.0	37.0	35.0	37.0
7	35.782120739737465	37.0	35.0	37.0	35.0	37.0
8	35.91759730137231	37.0	35.0	37.0	35.0	37.0
9	37.57408448544632	39.0	37.0	39.0	35.0	39.0
10	37.217534265245796	39.0	37.0	39.0	34.0	39.0
11	37.19262647687682	39.0	37.0	39.0	34.0	39.0
12	37.12412154046664	39.0	37.0	39.0	33.0	39.0
13	37.12132324755309	39.0	37.0	39.0	33.0	39.0
14	38.33803974683329	40.0	38.0	41.0	34.0	41.0
15	38.3854872096292	40.0	38.0	41.0	34.0	41.0
16	38.36453621595836	40.0	38.0	41.0	34.0	41.0
17	38.25407818183367	40.0	38.0	41.0	34.0	41.0
18	38.12704335011457	40.0	37.0	41.0	33.0	41.0
19	38.00727896893341	40.0	37.0	41.0	33.0	41.0
20	37.96170981233975	40.0	37.0	41.0	33.0	41.0
21	37.883336314771746	40.0	36.0	41.0	33.0	41.0
22	37.8193418687656	40.0	36.0	41.0	33.0	41.0
23	37.69340591006278	39.0	36.0	41.0	33.0	41.0
24	37.650549862427916	39.0	36.0	41.0	33.0	41.0
25	37.59468622490268	39.0	36.0	41.0	33.0	41.0
26	37.51322480897498	39.0	36.0	41.0	33.0	41.0
27	37.470453945294864	39.0	36.0	41.0	33.0	41.0
28	37.37213462472209	39.0	35.0	41.0	32.0	41.0
29	37.30516299949741	39.0	35.0	41.0	32.0	41.0
30	37.23344662799315	39.0	35.0	41.0	32.0	41.0
31	37.10650975782202	39.0	35.0	41.0	32.0	41.0
32	37.054087552068694	39.0	35.0	41.0	32.0	41.0
33	36.95474602403891	39.0	35.0	41.0	31.0	41.0
34	36.79886364604363	39.0	35.0	41.0	31.0	41.0
35	36.67161585443766	39.0	35.0	41.0	31.0	41.0
36	36.65076708151253	39.0	35.0	41.0	31.0	41.0
37	36.590729430204526	39.0	35.0	40.0	31.0	41.0
38	36.54015571626928	39.0	35.0	40.0	31.0	41.0
39	36.493014915710475	39.0	35.0	40.0	30.0	41.0
40	36.37998858535006	39.0	35.0	40.0	30.0	41.0
41	36.227032276200454	38.0	35.0	40.0	30.0	41.0
42	36.14962987571661	38.0	35.0	40.0	30.0	41.0
43	36.08043920847069	38.0	35.0	40.0	30.0	41.0
44	35.99780651316518	38.0	35.0	40.0	30.0	41.0
45	35.83954750283237	38.0	35.0	40.0	29.0	41.0
46	35.678626493913605	38.0	35.0	40.0	28.0	41.0
47	35.55634066767184	38.0	34.0	40.0	28.0	41.0
48	35.44510320036118	38.0	34.0	40.0	28.0	41.0
49	35.29598016917534	38.0	34.0	40.0	27.0	41.0
50	35.18156959955023	37.0	34.0	40.0	27.0	41.0
51	34.12950516640686	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	3.0
14	6.0
15	10.0
16	20.0
17	45.0
18	87.0
19	173.0
20	264.0
21	404.0
22	595.0
23	835.0
24	1094.0
25	1335.0
26	1605.0
27	1878.0
28	2470.0
29	3175.0
30	4021.0
31	5140.0
32	6891.0
33	9503.0
34	13995.0
35	20888.0
36	21759.0
37	30410.0
38	47585.0
39	60556.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.151601884269077	34.624296167573874	17.851575477243106	26.372526470913936
2	23.47967936759432	33.13144736057516	19.158723262886202	24.230150008944314
3	20.68010869472626	32.973005204739636	21.38330224118985	24.963583859344254
4	20.260577717581114	34.373855340608046	18.725988772754764	26.639578169056076
5	18.9930404708969	35.20695441806581	17.538950363309567	28.26105474772772
6	20.71929331391139	38.06998713722283	18.80819128908879	22.40252825977699
7	79.70108950278126	9.558917482303034	6.341093591611084	4.398899423304627
8	80.56442888417537	8.707929774347704	5.679214263201383	5.048427078275536
9	71.90633172335659	13.65115466850664	9.162812092714216	5.2797015154225555
10	31.119828269147227	36.92042966786776	15.77138330224119	16.188358760743824
11	23.54782653139455	32.77367475062397	26.94496264683584	16.73353607114564
12	24.053393302837478	30.46859693508131	28.413959946504473	17.064049815576738
13	22.853151380405986	31.11940234937347	26.643837366793594	19.38360890342695
14	19.00965134207321	30.95670099580043	29.6235721039585	20.410075558167865
15	18.77837690492619	32.97939400134591	28.287887693474055	19.954341400253846
16	20.58086938744218	32.87887693474057	26.858500932764308	19.68175274505294
17	20.202652628350922	32.66421336876986	27.875597352482686	19.25753665039653
18	21.243600555399386	31.194790149327474	27.181774041041628	20.379835254231512
19	20.41433475590538	34.52377910096855	26.91983338018451	18.14205276294157
20	21.976608486025572	33.07948514817749	27.171126046697847	17.772780319099095
21	21.48041194960517	34.55316756535739	25.560723382143742	18.405697102893697
22	21.83818455955636	32.08581431601544	27.165163169865323	18.91083795456288
23	20.81512526300546	34.113618358845926	24.615607404189348	20.455648973959264
24	18.50834376836779	33.33333333333333	27.87687511180394	20.281447786494937
25	19.783547570979533	34.73247979010674	25.439336246624585	20.044636392289146
26	22.172105662177472	33.35079604405714	25.88058913223105	18.596509161534332
27	19.418960244648318	33.269871287044374	25.096470828754697	22.214697639552615
28	19.877249921204843	34.967587505217516	25.685517875852902	19.469644697724735
29	20.916068249384544	34.509723748434745	24.616885163510602	19.95732283867011
30	20.446704658710484	34.5118533473035	25.12202601517978	19.91941597880623
31	20.642201834862387	36.66743332225942	23.498845757413132	19.19151908546506
32	21.52513352584907	33.279241522066904	25.571371376487523	19.6242535755965
33	20.63410935916111	33.78438237373608	24.61007044713058	20.97143781997223
34	19.843176339304726	34.695424769790364	26.841464141814246	18.61993474909066
35	20.440741781877968	34.52079766255228	26.303527467566205	18.734933088003544
36	21.484671147342688	36.04942373054612	24.77830875776239	17.687596364348813
37	20.70353428228259	35.10686327123423	25.186765820789997	19.002836625693185
38	21.32580307173341	34.10722956223965	26.62807833516479	17.938889030862146
39	21.033196187166187	32.20464593289208	26.149344509468197	20.61281337047354
40	22.98050139275766	32.08070327873042	25.32093054952169	19.617864778990228
41	18.966633444924312	33.04370788718237	28.464644399580898	19.52501426831242
42	21.47274539367765	32.74939732352014	25.71746185888426	20.06039542391795
43	19.261795848134046	32.34775497687256	27.31679060932083	21.07365856567257
44	21.18184218820543	32.11435094085678	26.503709761229377	20.200097109708416
45	21.354339696574755	32.58243677220959	26.462821462949236	19.60040206826642
46	18.762617873297387	32.66975032582863	28.26233250704897	20.305299293825016
47	19.155741824469942	33.75030879183597	27.914781971667818	19.17916741202627
48	21.148194526079067	32.08496247646793	28.030206230354448	18.73663676709855
49	20.2712257119249	33.046263405824874	26.72816948199637	19.954341400253846
50	19.29842494867667	31.78639271506819	26.745206272946426	22.169976063308717
51	19.46453366043972	30.25393336911059	28.70699275084545	21.574540219604234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6608.0
1	4867.0
2	3126.0
3	2008.0
4	890.0
5	712.0
6	534.0
7	619.0
8	704.0
9	719.5
10	735.0
11	754.0
12	773.0
13	878.0
14	983.0
15	951.5
16	920.0
17	936.5
18	953.0
19	1102.5
20	1252.0
21	1347.5
22	1443.0
23	1722.0
24	2001.0
25	2440.5
26	3566.0
27	4252.0
28	4315.0
29	4378.0
30	5385.5
31	6393.0
32	7034.0
33	7675.0
34	8482.5
35	9290.0
36	10095.5
37	10901.0
38	10603.0
39	10305.0
40	11260.5
41	12216.0
42	12892.0
43	13568.0
44	14638.5
45	15709.0
46	16247.0
47	16785.0
48	17624.0
49	18463.0
50	17606.0
51	16749.0
52	15492.5
53	14236.0
54	12578.0
55	10920.0
56	9704.0
57	8488.0
58	7700.5
59	6913.0
60	6480.5
61	6048.0
62	5772.5
63	5497.0
64	4702.0
65	3907.0
66	3046.0
67	2185.0
68	2179.5
69	2174.0
70	1819.5
71	1465.0
72	1209.0
73	953.0
74	802.5
75	568.0
76	484.0
77	340.0
78	196.0
79	133.0
80	70.0
81	68.5
82	67.0
83	42.0
84	17.0
85	18.0
86	19.0
87	12.5
88	6.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	234786.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.642005911766457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.08605349234766	24.3915736031961
2	8.613425583179659	5.450921264470624
3	3.2076563782961593	3.044900462548874
4	1.7673742445249088	2.236930651742438
5	1.1791468683959025	1.8655286090312029
6	0.8237875381943977	1.5639774092152003
7	0.6784132667483276	1.5026449617949962
8	0.5639983308879946	1.427683081614747
9	0.4415070466139909	1.2573151721141806
>10	4.818887886823438	31.443527297198298
>50	0.6084182471631826	13.304881892446737
>100	0.20460082647965433	8.684504186791377
>500	0.004038174206835283	0.9847265169132742
>1k	0.0013460580689450942	0.4310308110364332
>5k	0.0013460580689450942	2.4098540798855126
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5658	2.4098540798855126	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1012	0.4310308110364332	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	920	0.39184619185130287	No Hit
CTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGCT	874	0.3722538822587378	Illumina Single End Adapter 2 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	518	0.2206264428032336	No Hit
AATCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCT	347	0.1477941614917414	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGT	328	0.13970168579046452	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCC	326	0.13884984624296168	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	288	0.12266489484040786	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4246420144301619	0.0
2	0.0	0.0	0.0	0.6188614312608077	0.0
3	0.0	0.0	0.0	0.8211733237927304	0.0
4	0.0	0.0	0.0	1.7875852904346938	0.0
5	0.0	0.0	0.0	1.931120254188921	0.0
6	0.0	0.0	0.0	2.751015818660397	0.0
7	0.0	0.0	0.0	3.3085448025010007	0.0
8	0.0	0.0	0.0	3.8332779637627454	0.0
9	0.0	0.0	0.0	4.671913998279284	0.0
10	0.0	0.0	0.0	5.983746901433646	0.0
11	0.0	0.0	0.0	7.1908035402451596	0.0
12	0.0	0.0	0.0	7.689129675534316	0.0
13	0.0	0.0	0.0	7.907626519468793	0.0
14	0.0	0.0	0.0	8.155937747565869	0.0
15	0.0	0.0	0.0	8.379545628785362	0.0
16	0.0	0.0	0.0	8.83613162624688	0.0
17	0.0	0.0	0.0	9.36214254682988	0.0
18	0.0	0.0	0.0	10.016781239085805	0.0
19	0.0	0.0	0.0	10.320036117996814	0.0
20	0.0	0.0	0.0	10.629253873740343	0.0
21	0.0	0.0	0.0	10.995118959392808	0.0
22	0.0	0.0	0.0	11.376317156900326	0.0
23	0.0	0.0	0.0	11.742608162326544	0.0
24	0.0	0.0	0.0	12.049270399427563	0.0
25	0.0	0.0	0.0	12.332081129198505	0.0
26	0.0	0.0	0.0	12.598280987793139	0.0
27	0.0	0.0	0.0	12.882795396659086	0.0
28	0.0	0.0	0.0	13.13068070498241	0.0
29	0.0	0.0	0.0	13.411361835884593	0.0
30	0.0	0.0	0.0	13.76530116787202	0.0
31	0.0	0.0	0.0	14.037463903299175	0.0
32	0.0	0.0	0.0	14.31260807714259	0.0
33	0.0	0.0	0.0	14.590307769628513	0.0
34	0.0	0.0	0.0	14.862896424829419	0.0
35	0.0	0.0	0.0	15.136336919577829	0.0
36	0.0	0.0	0.0	15.424258686633786	0.0
37	0.0	0.0	0.0	15.711328614142241	0.0
38	0.0	0.0	0.0	15.99073198572317	0.0
39	0.0	0.0	0.0	16.280783351647884	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCACG	20	7.0218084E-4	45.0	39
AGGTACC	20	7.0218084E-4	45.0	6
AACCGCG	25	3.8812934E-5	45.0	1
AGTCCTA	20	7.0218084E-4	45.0	12
GTACCTA	20	7.0218084E-4	45.0	28
GCCCATT	25	3.8812934E-5	45.0	21
CTCACGG	40	6.7757355E-9	45.0	2
GGTACCA	20	7.0218084E-4	45.0	7
CGAACAG	20	7.0218084E-4	45.0	1
GCGACCG	20	7.0218084E-4	45.0	32
GATAACT	20	7.0218084E-4	45.0	9
GACACGT	20	7.0218084E-4	45.0	18
TAGACCC	20	7.0218084E-4	45.0	44
GTCCTAC	20	7.0218084E-4	45.0	13
CGTGACG	25	3.8812934E-5	45.0	1
GACCGAT	35	1.2064083E-7	45.0	9
GATCACT	25	3.8812934E-5	45.0	9
AGGGTGC	20	7.0218084E-4	45.0	6
AGGCGGT	20	7.0218084E-4	45.0	6
CCTCGGG	50	2.1827873E-11	45.0	3
>>END_MODULE