FastQCFastQC Report
Sat 18 Jun 2016
SRR3554204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283423
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98843.487366939168663No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19750.6968382947043817No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGT19670.6940156585739337No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCC18890.6664949563020643No Hit
AATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT17830.6290950275736267No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16540.5835800199701506No Hit
CTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGCT8890.3136654399960483No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8310.2932013280502994No Hit
AATGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT5380.18982227977263666No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT4690.16547704314752154No Hit
ATACGTGCGGGGTAACTTATTTATTTATTTATTTTTTGATTTCACAGTAGT4160.14677707878330268No Hit
AACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG3230.1139639337668432No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCTCATTC3200.11290544521792514No Hit
GGCTCTGGGGTCTTTTTTTTAAAAATTTTTATTCATTAAGGTGCCTCGTTT3100.109377150054865No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTAT3090.10902432053855897No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2990.10549602537549882No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGA207.0242834E-445.044
TATTTCG253.8833445E-545.045
GTTTGCG207.0242834E-445.039
GATACTC207.0242834E-445.015
AGTCCGC207.0242834E-445.039
AGTCCCC406.7830115E-945.028
AAATCGC207.0242834E-445.08
CGAACAG207.0242834E-445.01
TCCGCGG207.0242834E-445.02
CGTATAT207.0242834E-445.018
AGTCACG406.7830115E-945.01
GACACGG253.8833445E-545.02
TCACACG453.8198777E-1045.01
ACACCCG207.0242834E-445.01
GCTATGC207.0242834E-445.043
GACCGGT253.8833445E-545.018
TTCACGG207.0242834E-445.02
TCCCTCC207.0242834E-445.015
AGGACCG253.8833445E-545.01
CGCGCAG253.8833445E-545.01