##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554204_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	283423
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.056075195026516	33.0	31.0	34.0	30.0	34.0
2	32.24471196762436	34.0	31.0	34.0	30.0	34.0
3	32.33903741051361	34.0	31.0	34.0	30.0	34.0
4	35.886078405775116	37.0	35.0	37.0	35.0	37.0
5	35.69487656259372	37.0	35.0	37.0	33.0	37.0
6	35.65287926526781	37.0	35.0	37.0	33.0	37.0
7	35.56327115301158	37.0	35.0	37.0	35.0	37.0
8	35.76596818183422	37.0	35.0	37.0	35.0	37.0
9	37.4128810999813	39.0	37.0	39.0	35.0	39.0
10	37.016667666350294	39.0	37.0	39.0	33.0	39.0
11	36.980347395941756	39.0	37.0	39.0	33.0	39.0
12	36.911478602653986	39.0	37.0	39.0	33.0	39.0
13	36.91156681003306	39.0	37.0	39.0	33.0	39.0
14	38.063883312222366	40.0	37.0	41.0	33.0	41.0
15	38.118674207809526	40.0	37.0	41.0	33.0	41.0
16	38.055429517011675	40.0	37.0	41.0	33.0	41.0
17	37.963471560176835	40.0	37.0	41.0	33.0	41.0
18	37.76851208264679	39.0	37.0	41.0	33.0	41.0
19	37.5900509132992	39.0	36.0	41.0	32.0	41.0
20	37.563807453876365	39.0	36.0	41.0	32.0	41.0
21	37.531572949266646	39.0	36.0	41.0	32.0	41.0
22	37.48623082812616	39.0	35.0	41.0	32.0	41.0
23	37.298454959548096	39.0	35.0	41.0	32.0	41.0
24	37.25228016074913	39.0	35.0	41.0	32.0	41.0
25	37.20725911446848	39.0	35.0	41.0	32.0	41.0
26	37.092889426757885	39.0	35.0	41.0	32.0	41.0
27	37.00917356742396	39.0	35.0	41.0	31.0	41.0
28	36.93463833210431	39.0	35.0	41.0	31.0	41.0
29	36.75386965772009	39.0	35.0	41.0	31.0	41.0
30	36.71817742385057	39.0	35.0	41.0	31.0	41.0
31	36.60871206641663	39.0	35.0	40.0	31.0	41.0
32	36.5080709751855	39.0	35.0	40.0	31.0	41.0
33	36.49220070354205	39.0	35.0	40.0	31.0	41.0
34	36.25574847489442	38.0	35.0	40.0	30.0	41.0
35	36.33264766797331	38.0	35.0	40.0	30.0	41.0
36	36.16240036976534	38.0	35.0	40.0	30.0	41.0
37	36.115004780839946	38.0	35.0	40.0	30.0	41.0
38	36.051555448922635	38.0	35.0	40.0	30.0	41.0
39	35.88574321773462	38.0	35.0	40.0	29.0	41.0
40	35.8382170818882	38.0	35.0	40.0	29.0	41.0
41	35.73364899814059	38.0	35.0	40.0	29.0	41.0
42	35.66421214933156	38.0	35.0	40.0	29.0	41.0
43	35.57201074013047	38.0	35.0	40.0	28.0	41.0
44	35.47757944838634	38.0	34.0	40.0	28.0	41.0
45	35.2945667782784	38.0	34.0	40.0	27.0	41.0
46	35.11640551401968	37.0	34.0	40.0	26.0	41.0
47	34.97648744103337	37.0	34.0	40.0	26.0	41.0
48	34.889006890760456	37.0	34.0	40.0	25.0	41.0
49	34.75321339481976	37.0	33.0	40.0	26.0	41.0
50	34.60345490662367	37.0	33.0	40.0	24.0	41.0
51	33.53258204168328	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	0.0
13	4.0
14	6.0
15	17.0
16	35.0
17	77.0
18	150.0
19	260.0
20	458.0
21	728.0
22	1086.0
23	1349.0
24	1751.0
25	2121.0
26	2380.0
27	2783.0
28	3479.0
29	4406.0
30	5789.0
31	7437.0
32	9892.0
33	12864.0
34	18339.0
35	28219.0
36	27721.0
37	34820.0
38	51916.0
39	65314.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.193138877225913	37.924586219184754	17.73920959131757	24.143065312271762
2	20.769309477353637	37.73546959844472	18.416995092141427	23.078225832060205
3	18.697141728088404	34.50460971763054	22.612843700052572	24.185404854228484
4	21.9752807640876	34.07486336676981	19.552047646097883	24.397808223044706
5	17.557502390419973	37.649026366949755	18.476623280397146	26.31684796223313
6	20.178320037541063	37.58234158836792	21.22410672387209	21.01523165021893
7	77.23120565374016	12.947431930365566	5.6622080776789465	4.159154338215318
8	77.836308274205	10.62510805403937	4.858815269050147	6.679768402705497
9	71.88724979976925	14.989256341228483	8.090027979380643	5.033465879621626
10	29.222399029013168	45.04503868775646	13.290382220215013	12.442180063015352
11	20.988416606979673	32.479368294034	30.201853766278674	16.33036133270765
12	21.904714860826395	30.848237440151294	30.72086598476482	16.526181714257486
13	21.30208204697572	33.34627041559789	28.122276597171016	17.22937094025538
14	17.42766112841936	33.67757733140924	29.03469372633837	19.860067813833034
15	16.091848579684783	34.54836057765248	29.237570698214334	20.122220144448406
16	17.919858303666253	36.371077858889365	28.44723258168885	17.26183125575553
17	17.000384584172775	35.7282224801798	27.586681391418484	19.684711544228943
18	19.91546204789308	33.65570190139827	27.038031493562624	19.390804557146033
19	18.73665863391468	36.11951041376317	27.772622546511748	17.371208405810396
20	21.86555078451643	36.47622105474856	26.263923534787224	15.394304625947788
21	18.970231773709262	35.515466281847274	27.43143640424383	18.08286554019963
22	19.17945967687873	33.941493809606136	27.339700730004267	19.539345783510864
23	17.96008086852514	36.646990540640665	26.66861898999023	18.72430960084397
24	15.842398111656427	37.435564509584616	28.506507940428268	18.215529438330694
25	17.50881191716974	36.996997420816236	26.09103707179728	19.403153590216743
26	19.771507605240224	36.11951041376317	25.699396308697604	18.409585672299002
27	17.941028074644613	35.26107620059064	26.3094385423907	20.48845718237405
28	17.43118942358242	36.29663083094879	26.07974652727549	20.1924332181933
29	17.04166563758058	36.8950296906038	28.042537126485854	18.02076754532977
30	19.654015376310323	33.986303158177	26.794931956827778	19.564749508684898
31	19.703411508593163	36.20136686154617	24.991620298987733	19.103601330872934
32	19.21827092367239	32.839960059698754	28.405951528281047	19.535817488347806
33	17.613955113028936	33.94996171799748	25.18285389682559	23.25322927214799
34	16.472551627778973	34.4375721095324	29.569583273058292	19.52029298963034
35	16.482783683751848	33.81200537712183	29.640149176319497	20.06506176280683
36	16.12395606566863	37.85613729302138	27.880235548985087	18.1396710923249
37	18.83403958041514	34.93929568171955	26.79352063876256	19.433144099102755
38	17.262184085271837	35.02221061805146	28.22847828157912	19.487127015097574
39	16.047744890146532	34.56847186008193	28.004431538724806	21.37935171104674
40	19.526291091407543	33.81694499035011	28.219657543671474	18.43710637457087
41	16.058682605152015	34.629158536886564	30.014854122636482	19.29730473532494
42	19.481834572352984	33.53573986585422	27.73769242439745	19.244733137395343
43	18.23528789124383	33.78236769775212	28.38125346213963	19.601090948864417
44	20.00578640406742	32.97121263976459	25.947435458660728	21.07556549750726
45	20.519506179808978	33.2884063749237	26.028939076927422	20.163148368339904
46	15.58553822378565	34.076627514351344	29.91323922194035	20.42459503992266
47	18.105799458759524	34.805926124555874	27.020390017747324	20.067884398937277
48	18.096978720851872	34.06392565176433	29.289436637111315	18.54965899027249
49	19.254259534335606	33.56714169280545	27.895407218186246	19.283191554672698
50	17.433306400680255	33.93690702589416	27.23491036366138	21.394876209764206
51	17.109056075195024	32.13394819757042	28.904852464337754	21.8521432628968
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11091.0
1	8223.0
2	5355.0
3	3333.5
4	1312.0
5	1120.0
6	928.0
7	968.5
8	1009.0
9	1285.5
10	1562.0
11	1940.0
12	2318.0
13	2719.5
14	3121.0
15	3095.5
16	3070.0
17	2846.5
18	2623.0
19	2575.0
20	2527.0
21	2378.5
22	2230.0
23	2695.0
24	3160.0
25	3087.0
26	3801.5
27	4589.0
28	5578.0
29	6567.0
30	6403.5
31	6240.0
32	7641.0
33	9042.0
34	10111.0
35	11180.0
36	11981.0
37	12782.0
38	13557.5
39	14333.0
40	13826.0
41	13319.0
42	14406.0
43	15493.0
44	18708.0
45	21923.0
46	21455.0
47	20987.0
48	21149.5
49	21312.0
50	20000.0
51	18688.0
52	17267.0
53	15846.0
54	13866.0
55	11886.0
56	10516.5
57	9147.0
58	8056.5
59	6966.0
60	6294.5
61	5623.0
62	5197.0
63	4771.0
64	4048.0
65	3325.0
66	2612.5
67	1900.0
68	1686.5
69	1473.0
70	1200.0
71	927.0
72	811.5
73	696.0
74	589.0
75	373.0
76	264.0
77	197.5
78	131.0
79	142.0
80	153.0
81	89.0
82	25.0
83	21.0
84	17.0
85	14.0
86	11.0
87	6.5
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	283423.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.704364148287187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.79031702478947	22.810075399667635
2	8.488044756440866	5.042639447045582
3	3.403057406549549	3.0325696926502084
4	1.883856560833363	2.238350451445366
5	1.2804523156231813	1.9017510928894268
6	0.9229234222998255	1.6448912050186468
7	0.7780113791587975	1.6177233322630837
8	0.5998408343132713	1.425431245876305
9	0.5143189727874188	1.3749766250445448
>10	4.470892871990403	28.112044541198138
>50	0.631911532385466	13.207114454366794
>100	0.22568269013766643	10.038352568422464
>500	0.0035634108969105228	0.7966890478189843
>1k	0.005939018161517538	3.2700239571241574
>5k	0.0011878036323035076	3.487366939168663
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9884	3.487366939168663	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1975	0.6968382947043817	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGT	1967	0.6940156585739337	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCC	1889	0.6664949563020643	No Hit
AATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT	1783	0.6290950275736267	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1654	0.5835800199701506	No Hit
CTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGCT	889	0.3136654399960483	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	831	0.2932013280502994	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT	538	0.18982227977263666	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT	469	0.16547704314752154	No Hit
ATACGTGCGGGGTAACTTATTTATTTATTTATTTTTTGATTTCACAGTAGT	416	0.14677707878330268	No Hit
AACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	323	0.1139639337668432	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCTCATTC	320	0.11290544521792514	No Hit
GGCTCTGGGGTCTTTTTTTTAAAAATTTTTATTCATTAAGGTGCCTCGTTT	310	0.109377150054865	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTAT	309	0.10902432053855897	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	299	0.10549602537549882	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3965803763279621	0.0
2	0.0	0.0	0.0	0.5310084220405542	0.0
3	0.0	0.0	0.0	0.8302078518680559	0.0
4	0.0	0.0	0.0	2.2090656015919667	0.0
5	0.0	0.0	0.0	2.36819171344598	0.0
6	0.0	0.0	0.0	3.1666449088464943	0.0
7	0.0	0.0	0.0	3.793622959322285	0.0
8	0.0	0.0	0.0	4.229367411960215	0.0
9	0.0	0.0	0.0	5.6166930700754705	0.0
10	0.0	0.0	0.0	6.735868295798153	0.0
11	0.0	0.0	0.0	8.360295388871052	0.0
12	0.0	0.0	0.0	8.850022757503801	0.0
13	0.0	0.0	0.0	9.087829851494057	0.0
14	0.0	0.0	0.0	9.40008397342488	0.0
15	0.0	0.0	0.0	9.623072227730283	0.0
16	0.0	0.0	0.0	9.981547016297196	0.0
17	0.0	0.0	0.0	10.426465036359081	0.0
18	0.0	0.0	0.0	11.164231554954961	0.0
19	0.0	0.0	0.0	11.407331091689807	0.0
20	0.0	0.0	0.0	11.730522928626117	0.0
21	0.0	0.0	0.0	12.033603483132985	0.0
22	0.0	0.0	0.0	12.37231981878676	0.0
23	0.0	0.0	0.0	12.682104134103442	0.0
24	0.0	0.0	0.0	12.912854637767577	0.0
25	0.0	0.0	0.0	13.1136146325457	0.0
26	0.0	0.0	0.0	13.296733151508523	0.0
27	0.0	0.0	0.0	13.531364779852023	0.0
28	0.0	0.0	0.0	13.756117181738956	0.0
29	0.0	0.0	0.0	14.00556764976731	0.0
30	0.0	0.0	0.0	14.360866972687466	0.0
31	0.0	0.0	0.0	14.579974102313503	0.0
32	0.0	0.0	0.0	14.816017048722228	0.0
33	0.0	0.0	0.0	15.039358132543937	0.0
34	0.0	0.0	0.0	15.265521852496093	0.0
35	0.0	0.0	0.0	15.506151582616795	0.0
36	3.528295163060161E-4	0.0	0.0	15.742547358541826	0.0
37	3.528295163060161E-4	0.0	0.0	15.972239373657043	0.0
38	3.528295163060161E-4	0.0	0.0	16.228040772978904	0.0
39	3.528295163060161E-4	0.0	0.0	16.53570811119775	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGA	20	7.0242834E-4	45.0	44
TATTTCG	25	3.8833445E-5	45.0	45
GTTTGCG	20	7.0242834E-4	45.0	39
GATACTC	20	7.0242834E-4	45.0	15
AGTCCGC	20	7.0242834E-4	45.0	39
AGTCCCC	40	6.7830115E-9	45.0	28
AAATCGC	20	7.0242834E-4	45.0	8
CGAACAG	20	7.0242834E-4	45.0	1
TCCGCGG	20	7.0242834E-4	45.0	2
CGTATAT	20	7.0242834E-4	45.0	18
AGTCACG	40	6.7830115E-9	45.0	1
GACACGG	25	3.8833445E-5	45.0	2
TCACACG	45	3.8198777E-10	45.0	1
ACACCCG	20	7.0242834E-4	45.0	1
GCTATGC	20	7.0242834E-4	45.0	43
GACCGGT	25	3.8833445E-5	45.0	18
TTCACGG	20	7.0242834E-4	45.0	2
TCCCTCC	20	7.0242834E-4	45.0	15
AGGACCG	25	3.8833445E-5	45.0	1
CGCGCAG	25	3.8833445E-5	45.0	1
>>END_MODULE