FastQCFastQC Report
Sat 18 Jun 2016
SRR3554203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276874
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95053.4329695095964228No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18380.6638398693990769No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGT18040.651559915340552No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCC17950.6483093392662367No Hit
AATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT16820.60749655077761No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15630.5645167115727732No Hit
CTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGCT9470.34203283804185297No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8190.2958024227627007No Hit
AATGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT5060.18275461040039873No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT4660.16830760562566366No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCTCATTC3970.14338652238924565No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTAT3640.13146774345008921No Hit
ATACGTGCGGGGTAACTTATTTATTTATTTATTTTTTGATTTCACAGTAGT3560.1285783424951422No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3490.12605011665956356No Hit
AACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG3370.12171601522714304No Hit
GGCTCTGGGGTCTTTTTTTTAAAAATTTTTATTCATTAAGGTGCCTCGTTT2860.1032960841393558No Hit
CAAGGGGGGATCCTATTTACACATGAGTTTTTTTCCCACAAATATTTGTGG2810.10149020854251392No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA207.023999E-445.00000415
CTTGTCG207.023999E-445.00000411
TTTAGCG207.023999E-445.0000041
AACGTGT207.023999E-445.00000432
GATACAG207.023999E-445.0000041
TCTTGCG207.023999E-445.0000041
ACCGGTA207.023999E-445.00000419
TACCCCG207.023999E-445.0000041
TACCAGT207.023999E-445.00000416
TCCGATA207.023999E-445.00000431
CCGATAT207.023999E-445.00000432
CCGGCAG207.023999E-445.0000041
CATTGCG207.023999E-445.0000041
GACGTAA207.023999E-445.0000049
GACCGGT207.023999E-445.00000418
TTGTCGC207.023999E-445.00000412
GATCCAC207.023999E-445.00000442
GTTAGGT207.023999E-445.00000417
CGCTGGT207.023999E-445.0000043
TGCGGGC207.023999E-445.0000044