##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554203_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	276874
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12536027218157	33.0	31.0	34.0	30.0	34.0
2	32.30427559106308	34.0	31.0	34.0	30.0	34.0
3	32.39338832826484	34.0	31.0	34.0	30.0	34.0
4	35.919143726027	37.0	35.0	37.0	35.0	37.0
5	35.75150429437217	37.0	35.0	37.0	35.0	37.0
6	35.72570916734688	37.0	35.0	37.0	33.0	37.0
7	35.580303676040366	37.0	35.0	37.0	35.0	37.0
8	35.77942674285054	37.0	35.0	37.0	35.0	37.0
9	37.43374603610307	39.0	37.0	39.0	35.0	39.0
10	37.06794787520677	39.0	37.0	39.0	33.0	39.0
11	37.04098254079473	39.0	37.0	39.0	33.0	39.0
12	37.00957836416565	39.0	37.0	39.0	33.0	39.0
13	37.010102068088734	39.0	37.0	39.0	33.0	39.0
14	38.18837449525777	40.0	38.0	41.0	33.0	41.0
15	38.2321019669597	40.0	38.0	41.0	33.0	41.0
16	38.165400145914745	40.0	37.0	41.0	33.0	41.0
17	38.059785317509046	40.0	37.0	41.0	33.0	41.0
18	37.888234359311454	40.0	37.0	41.0	33.0	41.0
19	37.693900474584105	40.0	36.0	41.0	33.0	41.0
20	37.67447647666447	39.0	36.0	41.0	33.0	41.0
21	37.62694944270679	39.0	36.0	41.0	33.0	41.0
22	37.580401193322594	39.0	36.0	41.0	33.0	41.0
23	37.39660639857841	39.0	35.0	41.0	32.0	41.0
24	37.384655113878516	39.0	35.0	41.0	32.0	41.0
25	37.33357411674625	39.0	35.0	41.0	32.0	41.0
26	37.245884409514794	39.0	35.0	41.0	32.0	41.0
27	37.20446123507444	39.0	35.0	41.0	32.0	41.0
28	37.073954217441866	39.0	35.0	41.0	32.0	41.0
29	36.93659209604369	39.0	35.0	41.0	31.0	41.0
30	36.87910024054263	39.0	35.0	41.0	31.0	41.0
31	36.71646669604225	39.0	35.0	41.0	31.0	41.0
32	36.61206902778881	39.0	35.0	41.0	31.0	41.0
33	36.616934056646706	39.0	35.0	41.0	31.0	41.0
34	36.38764564386688	39.0	35.0	41.0	30.0	41.0
35	36.396216329449494	39.0	35.0	41.0	30.0	41.0
36	36.26881902959469	38.0	35.0	40.0	30.0	41.0
37	36.22738863165194	38.0	35.0	40.0	30.0	41.0
38	36.159603285248885	38.0	35.0	40.0	30.0	41.0
39	36.00925691830941	38.0	35.0	40.0	30.0	41.0
40	35.93018484942609	38.0	35.0	40.0	29.0	41.0
41	35.83907842556542	38.0	35.0	40.0	29.0	41.0
42	35.78459154705751	38.0	35.0	40.0	29.0	41.0
43	35.68099568756907	38.0	35.0	40.0	29.0	41.0
44	35.5941222361074	38.0	35.0	40.0	28.0	41.0
45	35.423452545201066	38.0	34.0	40.0	28.0	41.0
46	35.16129358480753	37.0	34.0	40.0	26.0	41.0
47	35.074344286570785	37.0	34.0	40.0	26.0	41.0
48	34.98425637654673	37.0	34.0	40.0	26.0	41.0
49	34.90318339750211	37.0	34.0	40.0	26.0	41.0
50	34.799623655525615	37.0	34.0	40.0	26.0	41.0
51	33.76985921393847	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	7.0
14	7.0
15	13.0
16	31.0
17	57.0
18	117.0
19	216.0
20	436.0
21	732.0
22	1006.0
23	1395.0
24	1749.0
25	1935.0
26	2283.0
27	2647.0
28	3190.0
29	4232.0
30	5471.0
31	6907.0
32	8989.0
33	11952.0
34	17141.0
35	27111.0
36	26381.0
37	33602.0
38	50950.0
39	68287.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.015241590037345	37.97864732694294	17.789319329370038	24.216791753649673
2	20.79032339620188	37.90894052890484	18.368644220836917	22.93209185405636
3	18.534423600627	34.57168242594104	22.441977217073468	24.45191675635849
4	22.11511373404509	34.10829474779141	19.26183029103491	24.514761227128584
5	17.493516906607336	37.90677347818863	18.398260580625124	26.201449034578907
6	20.147070508606806	37.450970477545745	21.229151166234463	21.172807847612994
7	77.31061782615919	12.849888396888115	5.567875640182899	4.271618136769794
8	77.83612762484017	10.593266251074496	4.867918258846984	6.70268786523834
9	71.83267479069902	15.142266879519203	8.087433272896696	4.937625056885081
10	29.378706559662515	45.05840201680187	13.19372711052681	12.369164313008806
11	20.7729869904722	32.411494036998775	30.183043550495892	16.632475422033128
12	22.079718572346987	30.878666830399386	30.31523364418472	16.726380953068904
13	21.187616027507097	33.57881202279737	28.069446751952153	17.164125197743378
14	17.580921285494487	33.846081611129975	28.600735352543033	19.97226175083251
15	16.21531815916265	34.57601652737346	29.121911049791606	20.086754263672287
16	17.870583731227924	36.3230205797583	28.553782587025147	17.252613101988633
17	17.08611137195981	35.584417460649966	27.459783150458332	19.86968801693189
18	19.61361485729971	33.6835528074142	27.146283146846578	19.556549188439508
19	18.756185123919185	36.096563779914334	27.808317140648814	17.33893395551767
20	21.884322832768696	36.35299811466587	26.274045233571954	15.488633818993478
21	18.942912660632636	35.4081640023982	27.786285458367345	17.86263787860182
22	19.240882134111544	33.85366628863671	27.125696165042584	19.779755412209163
23	17.879974284331503	36.817469318173615	26.677839017025796	18.624717380469093
24	15.665609627483981	37.38343073022386	28.78493466342091	18.166024978871256
25	17.34904685885999	37.087989482580525	26.175083250865015	19.387880407694475
26	19.777949536612322	36.00229707375918	25.81535283197411	18.404400557654384
27	17.93631760295297	35.46450732101967	26.15160686810607	20.447568207921293
28	17.433561836792187	36.37394627158924	26.28524166227237	19.9072502293462
29	17.146788792013695	36.94713118602685	27.833960574123967	18.072119447835476
30	19.38462983162016	34.32644451988992	26.77174454806157	19.517181100428356
31	19.726662669662012	36.09584142967559	25.042438076525787	19.135057824136613
32	19.23835390827597	32.87740994098399	28.434594797633583	19.44964135310647
33	17.456315869312395	34.03931029999205	25.38122033849332	23.123153492202228
34	16.343174151419056	34.42540650259685	29.691845388154903	19.53957395782919
35	16.4511655121102	33.803824122163874	29.676314858022064	20.068695507703865
36	16.275273228977802	37.78289041224528	27.827459421975338	18.114376936801577
37	18.823724871241073	34.99100673952773	26.700593049546	19.4846753396852
38	17.129452386284015	35.102248676293186	28.389086732593167	19.379212204829635
39	15.983804907647523	34.53195316281052	27.999378778794686	21.484863150747273
40	19.54426923438098	33.758677232242825	28.02899513858289	18.6680583947933
41	16.077710438683297	34.57890592832841	29.93961152004161	19.403772112946683
42	19.39546508520121	33.61745776056979	27.651567138842942	19.33551001538606
43	18.21875654629904	33.7897382925085	28.14746057773572	19.844044583456736
44	19.962148847490194	32.839125378330934	26.12162933319849	21.077096440980373
45	20.534250236569704	33.01140591026965	26.20253255993701	20.251811293223632
46	15.5706205710901	34.09204186741984	29.953697349696974	20.38364021179309
47	18.064173595209372	34.64716802588903	27.196486488438786	20.092171890462808
48	18.108236959772313	34.15163576211562	29.16452971387707	18.575597564234993
49	19.187789391564394	33.519940478340324	27.875856887970702	19.416413242124577
50	17.278617710583152	33.9017025795127	27.36045999263203	21.459219717272116
51	17.17243222548885	32.05248596834661	29.009585587668035	21.765496218496498
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10713.0
1	7870.5
2	5028.0
3	3168.0
4	1308.0
5	1091.5
6	875.0
7	971.0
8	1067.0
9	1329.0
10	1591.0
11	1962.0
12	2333.0
13	2755.0
14	3177.0
15	3078.0
16	2979.0
17	2902.0
18	2825.0
19	2692.0
20	2559.0
21	2350.0
22	2141.0
23	2530.0
24	2919.0
25	3017.0
26	3767.0
27	4419.0
28	5475.0
29	6531.0
30	6249.0
31	5967.0
32	7289.5
33	8612.0
34	9769.5
35	10927.0
36	11733.0
37	12539.0
38	13202.0
39	13865.0
40	13456.0
41	13047.0
42	14026.5
43	15006.0
44	18250.0
45	21494.0
46	20780.5
47	20067.0
48	20548.5
49	21030.0
50	19728.5
51	18427.0
52	17057.5
53	15688.0
54	13589.5
55	11491.0
56	10216.5
57	8942.0
58	7882.0
59	6822.0
60	6223.0
61	5624.0
62	5145.5
63	4667.0
64	3898.5
65	3130.0
66	2501.5
67	1873.0
68	1689.5
69	1506.0
70	1176.0
71	846.0
72	735.5
73	625.0
74	559.5
75	379.0
76	264.0
77	193.0
78	122.0
79	142.0
80	162.0
81	98.5
82	35.0
83	24.0
84	13.0
85	9.0
86	5.0
87	2.5
88	0.0
89	1.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	276874.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.55893294422734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.27594970735939	22.546356826570932
2	8.470082232621792	5.007331854923178
3	3.4371525274617856	3.0479568323497332
4	2.029545093535025	2.3996474930834966
5	1.46747962512677	2.1688565918071037
6	1.0129397245879204	1.7964850437383069
7	0.7612321452572671	1.5750846955654918
8	0.625603303967449	1.4793732889328721
9	0.4826431739592625	1.2839775493545802
>10	4.575946041715033	28.436039498111054
>50	0.6219376596082649	12.784154525163071
>100	0.22849183172248627	10.085454033242558
>500	0.0036656443591842718	0.8205898712049525
>1k	0.006109407265307121	3.1357223863562487
>5k	0.0012218814530614238	3.4329695095964228
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9505	3.4329695095964228	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1838	0.6638398693990769	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGT	1804	0.651559915340552	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCC	1795	0.6483093392662367	No Hit
AATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT	1682	0.60749655077761	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1563	0.5645167115727732	No Hit
CTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGCT	947	0.34203283804185297	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	819	0.2958024227627007	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT	506	0.18275461040039873	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT	466	0.16830760562566366	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCTCATTC	397	0.14338652238924565	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTAT	364	0.13146774345008921	No Hit
ATACGTGCGGGGTAACTTATTTATTTATTTATTTTTTGATTTCACAGTAGT	356	0.1285783424951422	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	349	0.12605011665956356	No Hit
AACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	337	0.12171601522714304	No Hit
GGCTCTGGGGTCTTTTTTTTAAAAATTTTTATTCATTAAGGTGCCTCGTTT	286	0.1032960841393558	No Hit
CAAGGGGGGATCCTATTTACACATGAGTTTTTTTCCCACAAATATTTGTGG	281	0.10149020854251392	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4037937834538454	0.0
2	0.0	0.0	0.0	0.5540426331110903	0.0
3	0.0	0.0	0.0	0.8512897563512645	0.0
4	0.0	0.0	0.0	2.179691845388155	0.0
5	0.0	0.0	0.0	2.353055902684976	0.0
6	0.0	0.0	0.0	3.1411400131467744	0.0
7	0.0	0.0	0.0	3.749358914163121	0.0
8	0.0	0.0	0.0	4.200466638254224	0.0
9	0.0	0.0	0.0	5.522367575142484	0.0
10	0.0	0.0	0.0	6.6997984642833925	0.0
11	0.0	0.0	0.0	8.298720717727198	0.0
12	0.0	0.0	0.0	8.78341772791956	0.0
13	0.0	0.0	0.0	9.043102638745422	0.0
14	0.0	0.0	0.0	9.382968426071065	0.0
15	0.0	0.0	0.0	9.596061746498407	0.0
16	0.0	0.0	0.0	9.97348974623836	0.0
17	0.0	0.0	0.0	10.415568092345255	0.0
18	0.0	0.0	0.0	11.20401337792642	0.0
19	0.0	0.0	0.0	11.479228818885124	0.0
20	0.0	0.0	0.0	11.802480550719823	0.0
21	0.0	0.0	0.0	12.12789933327073	0.0
22	0.0	0.0	0.0	12.467765120596372	0.0
23	0.0	0.0	0.0	12.798962705057175	0.0
24	0.0	0.0	0.0	13.016390126916937	0.0
25	0.0	0.0	0.0	13.21359174209207	0.0
26	0.0	0.0	0.0	13.404653380237942	0.0
27	0.0	0.0	0.0	13.635444281514335	0.0
28	0.0	0.0	0.0	13.867318708148833	0.0
29	0.0	0.0	0.0	14.117613065871119	0.0
30	0.0	0.0	0.0	14.466147056061603	0.0
31	0.0	0.0	0.0	14.710662611873992	0.0
32	0.0	0.0	0.0	14.943259388747228	0.0
33	0.0	0.0	0.0	15.163937386681306	0.0
34	3.6117511936837693E-4	0.0	0.0	15.38533773485412	0.0
35	3.6117511936837693E-4	0.0	0.0	15.623352138517882	0.0
36	3.6117511936837693E-4	0.0	0.0	15.874007671359536	0.0
37	3.6117511936837693E-4	0.0	0.0	16.112022075023297	0.0
38	3.6117511936837693E-4	0.0	0.0	16.363761133223054	0.0
39	3.6117511936837693E-4	0.0	0.0	16.67870583731228	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	20	7.023999E-4	45.000004	15
CTTGTCG	20	7.023999E-4	45.000004	11
TTTAGCG	20	7.023999E-4	45.000004	1
AACGTGT	20	7.023999E-4	45.000004	32
GATACAG	20	7.023999E-4	45.000004	1
TCTTGCG	20	7.023999E-4	45.000004	1
ACCGGTA	20	7.023999E-4	45.000004	19
TACCCCG	20	7.023999E-4	45.000004	1
TACCAGT	20	7.023999E-4	45.000004	16
TCCGATA	20	7.023999E-4	45.000004	31
CCGATAT	20	7.023999E-4	45.000004	32
CCGGCAG	20	7.023999E-4	45.000004	1
CATTGCG	20	7.023999E-4	45.000004	1
GACGTAA	20	7.023999E-4	45.000004	9
GACCGGT	20	7.023999E-4	45.000004	18
TTGTCGC	20	7.023999E-4	45.000004	12
GATCCAC	20	7.023999E-4	45.000004	42
GTTAGGT	20	7.023999E-4	45.000004	17
CGCTGGT	20	7.023999E-4	45.000004	3
TGCGGGC	20	7.023999E-4	45.000004	4
>>END_MODULE