FastQCFastQC Report
Sat 18 Jun 2016
SRR3554201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724650
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45590.629131304767819No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA23540.3248464776098806No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9180.12668184640861105No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8850.12212792382529497No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA7900.10901814669150624No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7840.10819016076726695No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCG351.2111013E-745.0000041
CGAGTAT207.031558E-445.00000437
TTCGTAC207.031558E-445.00000433
ATCGTTA207.031558E-445.00000440
AATTGCG406.8084773E-945.0000041
CGCTATA207.031558E-445.00000433
AAACCGC207.031558E-445.00000443
CGTGTTA207.031558E-445.00000418
ACCCGTC207.031558E-445.00000435
TTGCACG207.031558E-445.0000041
TATACGA207.031558E-445.00000434
TAAGCGA207.031558E-445.00000423
GCCGTTT351.2111013E-745.0000049
CGCTTGT207.031558E-445.00000415
TGCGTGT207.031558E-445.00000443
AGTAACG406.8084773E-945.0000041
CGGAATA253.889374E-545.032
ACACGAA253.889374E-545.026
TAACGTC253.889374E-545.032
TATGCGG453.8380676E-1045.02