##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554201_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	724650
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49305871800179	34.0	31.0	34.0	31.0	34.0
2	32.65532601945767	34.0	31.0	34.0	31.0	34.0
3	32.73811633202236	34.0	31.0	34.0	31.0	34.0
4	36.211253708686954	37.0	37.0	37.0	35.0	37.0
5	36.07415027944525	37.0	35.0	37.0	35.0	37.0
6	36.05941488994687	37.0	35.0	37.0	35.0	37.0
7	36.065926999241015	37.0	36.0	37.0	35.0	37.0
8	36.17948802870351	37.0	37.0	37.0	35.0	37.0
9	37.85143172566066	39.0	38.0	39.0	35.0	39.0
10	37.48461326157455	39.0	37.0	39.0	35.0	39.0
11	37.456550058649	39.0	37.0	39.0	35.0	39.0
12	37.404657420823845	39.0	37.0	39.0	35.0	39.0
13	37.38808804250328	39.0	37.0	39.0	35.0	39.0
14	38.68534878907059	40.0	38.0	41.0	35.0	41.0
15	38.68417856896433	40.0	38.0	41.0	35.0	41.0
16	38.69172014075761	40.0	38.0	41.0	35.0	41.0
17	38.657877596080866	40.0	38.0	41.0	35.0	41.0
18	38.58229352101014	40.0	38.0	41.0	34.0	41.0
19	38.57449665355689	40.0	38.0	41.0	34.0	41.0
20	38.489542537776856	40.0	38.0	41.0	34.0	41.0
21	38.450405023114605	40.0	38.0	41.0	34.0	41.0
22	38.40231698061133	40.0	38.0	41.0	34.0	41.0
23	38.331026012557786	40.0	38.0	41.0	34.0	41.0
24	38.288904988615194	40.0	38.0	41.0	34.0	41.0
25	38.2362699234113	40.0	37.0	41.0	34.0	41.0
26	38.16111778099772	40.0	37.0	41.0	34.0	41.0
27	38.08899468709032	40.0	37.0	41.0	34.0	41.0
28	37.98863451321328	40.0	37.0	41.0	33.0	41.0
29	37.920020699648106	40.0	37.0	41.0	33.0	41.0
30	37.8881708410957	40.0	37.0	41.0	33.0	41.0
31	37.83260056579038	40.0	37.0	41.0	33.0	41.0
32	37.76337542261781	40.0	37.0	41.0	33.0	41.0
33	37.7022714413855	40.0	37.0	41.0	33.0	41.0
34	37.60290071068792	40.0	36.0	41.0	33.0	41.0
35	37.51852894500794	40.0	36.0	41.0	33.0	41.0
36	37.42957151728421	40.0	36.0	41.0	33.0	41.0
37	37.362356999931	40.0	36.0	41.0	32.0	41.0
38	37.308208100462295	40.0	36.0	41.0	32.0	41.0
39	37.24021665631684	40.0	35.0	41.0	32.0	41.0
40	37.15398882219002	39.0	35.0	41.0	32.0	41.0
41	37.02832263851514	39.0	35.0	41.0	31.0	41.0
42	36.95667011660802	39.0	35.0	41.0	31.0	41.0
43	36.886585248050785	39.0	35.0	41.0	31.0	41.0
44	36.77912371489685	39.0	35.0	41.0	31.0	41.0
45	36.63097633340233	39.0	35.0	41.0	31.0	41.0
46	36.46832263851515	39.0	35.0	41.0	30.0	41.0
47	36.386603187745806	39.0	35.0	40.0	30.0	41.0
48	36.27696543158766	39.0	35.0	40.0	30.0	41.0
49	36.159650865935276	38.0	35.0	40.0	30.0	41.0
50	36.066512109294145	38.0	35.0	40.0	29.0	41.0
51	35.088900848685576	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	3.0
11	1.0
12	5.0
13	15.0
14	19.0
15	25.0
16	53.0
17	96.0
18	183.0
19	317.0
20	471.0
21	744.0
22	1099.0
23	1584.0
24	2200.0
25	2671.0
26	3667.0
27	4576.0
28	5885.0
29	7395.0
30	9738.0
31	12738.0
32	16822.0
33	23282.0
34	37496.0
35	49042.0
36	56937.0
37	85624.0
38	150452.0
39	251373.0
40	133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.219071275788313	32.54329676395501	20.40488511695301	25.83274684330366
2	22.200924584282067	31.66066376871593	20.520665148692473	25.61774649830953
3	21.15559235492997	31.624370385703443	21.40895604774719	25.811081211619403
4	23.13696267163458	30.485337749258264	19.541709790933552	26.835989788173602
5	19.50072448768371	34.750431242668874	16.922238321948527	28.82660594769889
6	21.13682467398054	37.80004139929621	19.44138549644656	21.621748430276686
7	86.0376733595529	6.385427447733388	3.903815635134203	3.6730835575795213
8	87.10163527220037	5.832608845649625	3.7252466708065963	3.340509211343407
9	77.90215966328572	11.387152418408887	6.250327744428345	4.460360173877044
10	32.83861174360036	40.916856413440975	13.405644104050232	12.838887738908438
11	25.135858690402262	28.961015662733736	27.730904574622233	18.172221072241772
12	27.08272959359691	25.355964948595872	29.29110605119713	18.27019940661009
13	24.540812806182295	27.449527358034914	28.02815152142414	19.981508314358656
14	20.285241150900436	28.568136341682195	28.599599806803283	22.54702270061409
15	20.45304629821293	31.038432346650108	29.001448975367417	19.507072379769543
16	23.08231560063479	28.564134409715038	28.35975988408197	19.993790105568205
17	21.6494859587387	29.061891947836887	29.050852135513693	20.237769957910714
18	22.11688401297178	29.281860208376457	28.023873594148903	20.577382184502863
19	23.295107983164286	28.853653487890707	27.877182087904508	19.974056441040503
20	24.45346029117505	28.04553922583316	28.79210653418892	18.70889394880287
21	23.982750293245015	28.217622300420892	27.596908852549507	20.202718553784585
22	22.75622714413855	28.706823983992276	27.821845028634513	20.715103843234665
23	22.51224729179604	28.703236044987236	28.38887738908439	20.39563927413234
24	21.516870213206378	28.595183881874007	29.341475194921685	20.54647070999793
25	22.040709307941764	30.19719864762299	27.020078658662804	20.74201338577244
26	22.7221417235907	30.356861933347133	26.595735872490167	20.325260470572
27	21.42924170289105	30.366383771475885	27.139584627061343	21.064789898571725
28	21.798247429793694	30.120333954322774	28.622783412681983	19.458635203201545
29	21.75726212654385	29.483750776236807	27.305457807217277	21.45352929000207
30	21.972124473883945	29.525840060718966	28.260677568481334	20.24135789691575
31	23.33705926999241	29.486924722279724	25.910853515490235	21.265162492237632
32	22.340026219554268	29.657489822673018	27.61471054992065	20.387773407852066
33	20.828123921893326	30.983371282688193	27.37307665769682	20.81542813772166
34	21.89801973366453	29.130890774856827	28.666804664320704	20.30428482715794
35	23.123990892154833	28.718139791623543	28.39453529290002	19.763334023321605
36	22.28869109225143	31.24253087697509	27.406058097012355	19.062719933761127
37	21.458083212585386	31.953908783550677	27.658593803905333	18.9294141999586
38	20.85020354653971	29.93886703926033	29.17146208514455	20.039467329055405
39	21.899951700821084	29.359138894638793	27.54350376043607	21.19740564410405
40	22.610501621472434	29.230525081073623	28.503553439591528	19.655419857862416
41	19.644380045539226	30.02442558476506	30.887048920168358	19.44414544952736
42	20.859587387014418	29.60505071413786	28.34885806941282	21.186503829434898
43	21.44373145656524	29.37293866004278	27.936659076795696	21.24667080659629
44	21.077761678051473	30.05685503346443	27.58421306837784	21.281170220106258
45	21.397364244807836	29.692127233837024	28.045401228179124	20.865107293176017
46	20.188090802456358	30.21748430276685	28.149865452287308	21.444559442489478
47	21.52294210998413	29.560615469537016	29.06492789622576	19.85151452425309
48	21.112675084523563	28.33892223832195	29.675291520044162	20.87311115711033
49	19.947698889118882	30.086662526737047	28.955633754226177	21.010004829917893
50	19.24446284413165	28.940592009935834	29.588077002690955	22.226868143241564
51	20.644311046712204	27.73490650658939	29.011522804112328	22.60925964258608
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5900.0
1	4624.0
2	3348.0
3	2231.5
4	1115.0
5	1050.5
6	986.0
7	1003.5
8	1021.0
9	1112.5
10	1204.0
11	1283.5
12	1363.0
13	1566.0
14	1769.0
15	1880.5
16	1992.0
17	2231.0
18	2470.0
19	2709.5
20	2949.0
21	3499.0
22	4049.0
23	5304.0
24	6559.0
25	7469.5
26	10182.5
27	11985.0
28	12688.0
29	13391.0
30	18127.0
31	22863.0
32	24611.0
33	26359.0
34	28925.0
35	31491.0
36	34143.0
37	36795.0
38	38232.0
39	39669.0
40	41818.0
41	43967.0
42	43368.0
43	42769.0
44	44543.0
45	46317.0
46	46983.5
47	47650.0
48	47879.0
49	48108.0
50	46889.0
51	45670.0
52	42236.5
53	38803.0
54	35814.0
55	32825.0
56	30943.0
57	29061.0
58	27780.5
59	26500.0
60	26212.0
61	25924.0
62	23579.0
63	21234.0
64	18311.5
65	15389.0
66	13195.0
67	11001.0
68	9737.5
69	8474.0
70	7000.0
71	5526.0
72	4658.5
73	3791.0
74	3243.5
75	2078.0
76	1460.0
77	1165.0
78	870.0
79	654.0
80	438.0
81	343.5
82	249.0
83	172.5
84	96.0
85	94.0
86	92.0
87	64.5
88	37.0
89	27.5
90	18.0
91	15.5
92	13.0
93	9.5
94	6.0
95	5.0
96	4.0
97	3.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	724650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.607958140772293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.52237186476506	15.151459617993726
2	8.079143705730038	3.329893106019371
3	3.067103560520329	1.8962022536584977
4	1.7723267152909603	1.4609613904195424
5	1.2196681851712072	1.2567435452819975
6	0.8905383901248214	1.1011306719865839
7	0.7448351327000589	1.0744671865521571
8	0.6718415198089981	1.1076225533965336
9	0.5551707873209565	1.0296842711480882
>10	7.714786543233457	36.510193113575525
>50	1.1992189354446923	17.128569347092117
>100	0.5542608525164545	16.957582707177647
>500	0.007390144700219392	1.0383804043388962
>1k	0.0013436626727671623	0.9571098313593053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4559	0.629131304767819	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2354	0.3248464776098806	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	918	0.12668184640861105	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	885	0.12212792382529497	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	790	0.10901814669150624	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	784	0.10819016076726695	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11509004346926102	0.0
2	0.0	0.0	0.0	0.16794314496653556	0.0
3	0.0	0.0	0.0	0.22259021596632858	0.0
4	0.0	0.0	0.0	0.4771958876699096	0.0
5	0.0	0.0	0.0	0.5224591181949907	0.0
6	0.0	0.0	0.0	0.8088042503277444	0.0
7	0.0	0.0	0.0	1.015110743117367	0.0
8	0.0	0.0	0.0	1.2334230318084594	0.0
9	0.0	0.0	0.0	1.5335679293452011	0.0
10	0.0	0.0	0.0	2.015179741944387	0.0
11	0.0	0.0	0.0	2.424618781480715	0.0
12	0.0	0.0	0.0	2.610777616780515	0.0
13	0.0	0.0	0.0	2.6973021458635205	0.0
14	0.0	0.0	0.0	2.798592423928793	0.0
15	0.0	0.0	0.0	2.8913268474435934	0.0
16	0.0	0.0	0.0	3.0507141378596563	0.0
17	0.0	0.0	0.0	3.2491547643690057	0.0
18	0.0	0.0	0.0	3.4841647691989235	0.0
19	0.0	0.0	0.0	3.6017387704409023	0.0
20	0.0	0.0	0.0	3.7351825018974676	0.0
21	0.0	0.0	0.0	3.881460015179742	0.0
22	0.0	0.0	0.0	4.052163113227075	0.0
23	0.0	0.0	0.0	4.216242323880494	0.0
24	0.0	0.0	0.0	4.347202097564342	0.0
25	0.0	0.0	0.0	4.470847995584075	0.0
26	0.0	0.0	0.0	4.583730076588698	0.0
27	0.0	0.0	0.0	4.703098047333195	0.0
28	0.0	0.0	0.0	4.827847926585248	0.0
29	0.0	0.0	0.0	4.958807700269095	0.0
30	0.0	0.0	0.0	5.125370868695232	0.0
31	0.0	0.0	0.0	5.249430759677085	0.0
32	0.0	0.0	0.0	5.384530462982129	0.0
33	0.0	0.0	0.0	5.525564065410888	0.0
34	0.0	0.0	0.0	5.682881391016353	0.0
35	0.0	0.0	0.0	5.829848892568826	0.0
36	0.0	0.0	0.0	5.972952459808183	0.0
37	0.0	0.0	0.0	6.1298557924515285	0.0
38	0.0	0.0	0.0	6.288139101635272	0.0
39	0.0	0.0	0.0	6.436072586766025	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGCG	35	1.2111013E-7	45.000004	1
CGAGTAT	20	7.031558E-4	45.000004	37
TTCGTAC	20	7.031558E-4	45.000004	33
ATCGTTA	20	7.031558E-4	45.000004	40
AATTGCG	40	6.8084773E-9	45.000004	1
CGCTATA	20	7.031558E-4	45.000004	33
AAACCGC	20	7.031558E-4	45.000004	43
CGTGTTA	20	7.031558E-4	45.000004	18
ACCCGTC	20	7.031558E-4	45.000004	35
TTGCACG	20	7.031558E-4	45.000004	1
TATACGA	20	7.031558E-4	45.000004	34
TAAGCGA	20	7.031558E-4	45.000004	23
GCCGTTT	35	1.2111013E-7	45.000004	9
CGCTTGT	20	7.031558E-4	45.000004	15
TGCGTGT	20	7.031558E-4	45.000004	43
AGTAACG	40	6.8084773E-9	45.000004	1
CGGAATA	25	3.889374E-5	45.0	32
ACACGAA	25	3.889374E-5	45.0	26
TAACGTC	25	3.889374E-5	45.0	32
TATGCGG	45	3.8380676E-10	45.0	2
>>END_MODULE