##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554199_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335900
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20308127418875	33.0	31.0	34.0	30.0	34.0
2	32.381607621315865	34.0	31.0	34.0	30.0	34.0
3	32.47317058648407	34.0	31.0	34.0	30.0	34.0
4	36.00171777314677	37.0	35.0	37.0	35.0	37.0
5	35.844763322417386	37.0	35.0	37.0	35.0	37.0
6	35.82185174158976	37.0	35.0	37.0	35.0	37.0
7	35.82591247395058	37.0	35.0	37.0	35.0	37.0
8	35.9363560583507	37.0	35.0	37.0	35.0	37.0
9	37.61216135754689	39.0	37.0	39.0	35.0	39.0
10	37.19518606728193	39.0	37.0	39.0	34.0	39.0
11	37.16913069365883	39.0	37.0	39.0	34.0	39.0
12	37.19023518904436	39.0	37.0	39.0	34.0	39.0
13	37.20529026495981	39.0	37.0	39.0	33.0	39.0
14	38.40701101518309	40.0	38.0	41.0	34.0	41.0
15	38.438097648109554	40.0	38.0	41.0	34.0	41.0
16	38.40983328371539	40.0	38.0	41.0	34.0	41.0
17	38.362119678475736	40.0	38.0	41.0	34.0	41.0
18	38.2067401012206	40.0	37.0	41.0	34.0	41.0
19	38.123766001786244	40.0	37.0	41.0	34.0	41.0
20	38.03501041976779	40.0	37.0	41.0	33.0	41.0
21	38.00436737124144	40.0	37.0	41.0	33.0	41.0
22	37.9662191128312	40.0	37.0	41.0	33.0	41.0
23	37.84371539148556	40.0	37.0	41.0	33.0	41.0
24	37.81613277761238	40.0	36.0	41.0	33.0	41.0
25	37.76833283715391	40.0	36.0	41.0	33.0	41.0
26	37.66380172670438	40.0	36.0	41.0	33.0	41.0
27	37.622521583804705	40.0	36.0	41.0	33.0	41.0
28	37.51197082465019	40.0	36.0	41.0	33.0	41.0
29	37.45133968442989	40.0	36.0	41.0	33.0	41.0
30	37.39182197082465	40.0	36.0	41.0	32.0	41.0
31	37.25755879726109	39.0	36.0	41.0	32.0	41.0
32	37.21788032152426	39.0	36.0	41.0	32.0	41.0
33	37.14228639476035	39.0	35.0	41.0	32.0	41.0
34	37.071220601369454	39.0	35.0	41.0	31.0	41.0
35	36.945882703185475	39.0	35.0	41.0	31.0	41.0
36	36.88014289967252	39.0	35.0	41.0	31.0	41.0
37	36.775028282226856	39.0	35.0	41.0	31.0	41.0
38	36.772134563858295	39.0	35.0	41.0	31.0	41.0
39	36.71735337898184	39.0	35.0	41.0	31.0	41.0
40	36.663245013396846	39.0	35.0	41.0	31.0	41.0
41	36.52937183685621	39.0	35.0	41.0	30.0	41.0
42	36.42465614766299	39.0	35.0	41.0	30.0	41.0
43	36.30027984519202	39.0	35.0	40.0	30.0	41.0
44	36.27284013099137	39.0	35.0	40.0	30.0	41.0
45	36.10153617147961	39.0	35.0	40.0	29.0	41.0
46	35.93811551056862	38.0	35.0	40.0	28.0	41.0
47	35.869627865436144	38.0	35.0	40.0	28.0	41.0
48	35.79652872878833	38.0	35.0	40.0	28.0	41.0
49	35.66419470080381	38.0	35.0	40.0	28.0	41.0
50	35.5653140815719	38.0	34.0	40.0	27.0	41.0
51	34.48443286692468	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	1.0
11	2.0
12	2.0
13	4.0
14	6.0
15	14.0
16	27.0
17	62.0
18	113.0
19	235.0
20	374.0
21	623.0
22	840.0
23	1122.0
24	1511.0
25	1890.0
26	2357.0
27	2768.0
28	3328.0
29	4380.0
30	5634.0
31	7332.0
32	9364.0
33	12751.0
34	17927.0
35	26967.0
36	28135.0
37	40943.0
38	68130.0
39	99012.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.003870199464124	33.92855016373921	19.35933313486157	25.7082465019351
2	21.880321524263174	33.79130693658827	19.60553736231021	24.722834176838347
3	20.355760643048527	34.272104793093185	21.44894313783864	23.92319142601965
4	19.203929740994344	34.3295623697529	20.440905031259305	26.02560285799345
5	18.723727299791605	35.316165525454004	18.03185471866627	27.92825245608812
6	20.727299791604644	38.133373027686815	20.661804108365587	20.477523072342958
7	81.84519202143495	8.390592438225662	5.123250967549866	4.640964572789521
8	82.29115808276273	9.169395653468294	4.600178624590652	3.939267639178327
9	73.94671033045549	13.516820482286395	8.105388508484667	4.431080678773444
10	28.43495087823757	45.22447156891932	13.860077403989282	12.480500148853825
11	20.09199166418577	30.556415599880914	32.473355165227744	16.87823757070557
12	22.301875558201843	28.32003572491813	32.18130395951176	17.196784757368263
13	22.552843108067876	28.309913664781185	30.98511461744567	18.15212860970527
14	18.56921702887764	31.14051801131289	31.27478416195296	19.015480797856505
15	17.993450431676095	33.60762131586782	29.71598690086335	18.682941351592735
16	19.160762131586782	33.1092587079488	28.753498064900267	18.976481095564157
17	19.74784161952962	30.59124739505805	31.758261387317653	17.902649598094673
18	19.59154510270914	29.582018457874366	30.14617445668354	20.680261982732954
19	18.837153914855612	32.759452217922004	30.329562369752907	18.073831497469484
20	20.332837153914856	31.509080083358143	30.779100922893722	17.378981839833283
21	20.835069961298007	31.342959214051803	29.394164930038702	18.42780589461149
22	19.927061625483773	30.55790413813635	29.80797856504912	19.707055671330753
23	18.966656743078296	33.473355165227744	27.943733253944625	19.61625483774933
24	16.40934802024412	33.41708841917237	29.43792795474844	20.73563560583507
25	18.641559988091693	32.538553140815715	29.089907710628164	19.729979160464424
26	19.958916344150044	33.471866626972314	28.77433760047633	17.794879428401313
27	18.418576957427806	31.73027686811551	28.845787436737126	21.00535873771956
28	18.446263768978863	32.384638285203934	30.2738910389997	18.895206906817506
29	19.168502530515035	31.531408157189638	29.148853825543313	20.151235486752007
30	20.59571300982435	31.428996725215836	28.35963084251265	19.615659422447155
31	19.094075617743375	32.753795772551356	27.83209288478714	20.32003572491813
32	19.88151235486752	31.234593629056267	27.899672521583806	20.98422149449241
33	18.854718666269722	31.47603453408753	29.080976481095565	20.588270318547185
34	19.609109854123254	29.719261685025305	30.690979458172073	19.980649002679367
35	18.987198571003276	30.556713307532	30.52247692765704	19.933611193807682
36	20.18219708246502	31.150937779100925	29.636796665674307	19.03006847275975
37	17.98868710925871	32.4164930038702	30.089014587674907	19.50580529919619
38	18.855611789222984	31.690086335218815	29.724322715093777	19.729979160464424
39	18.467996427508186	29.99374813932718	29.869008633521883	21.66924679964275
40	20.44924084548973	31.136647811848768	28.592735933313485	19.821375409348022
41	18.21494492408455	29.62756772849062	31.85263471271212	20.30485263471271
42	20.353974397142007	30.335516522774636	29.953855314081572	19.356653766001784
43	18.2828222685323	31.22744864543019	30.652872878832987	19.836856207204526
44	20.458172075022325	29.441202738910388	29.2917534980649	20.80887168800238
45	19.696635903542724	31.406966359035426	30.023221196784753	18.873176540637093
46	17.765704078594823	31.36201250372135	30.28847871390295	20.583804703780885
47	18.459362905626676	30.366478118487645	30.074724620422742	21.099434355462936
48	18.66835367668949	30.188448943137843	31.09883894016076	20.04435844001191
49	17.87823757070557	31.0357249181304	30.690681750520987	20.39535576064305
50	18.05209883894016	29.852039297409945	29.709437332539444	22.38642453111045
51	18.566239952366775	28.11521286097053	31.967847573682644	21.350699612980055
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8257.0
1	6225.5
2	4194.0
3	2705.0
4	1216.0
5	957.0
6	698.0
7	740.5
8	783.0
9	840.5
10	898.0
11	958.5
12	1019.0
13	1082.0
14	1145.0
15	1326.0
16	1507.0
17	1345.0
18	1183.0
19	1387.5
20	1592.0
21	1803.0
22	2014.0
23	3092.5
24	4171.0
25	4379.0
26	5891.5
27	7196.0
28	7692.5
29	8189.0
30	9044.5
31	9900.0
32	10640.5
33	11381.0
34	13639.5
35	15898.0
36	17851.0
37	19804.0
38	19081.5
39	18359.0
40	19248.0
41	20137.0
42	20014.0
43	19891.0
44	21438.5
45	22986.0
46	23878.0
47	24770.0
48	26644.0
49	28518.0
50	26228.5
51	23939.0
52	21347.0
53	18755.0
54	16429.0
55	14103.0
56	12135.0
57	10167.0
58	8670.0
59	7173.0
60	6602.0
61	6031.0
62	5672.0
63	5313.0
64	4351.5
65	3390.0
66	2800.0
67	2210.0
68	1855.5
69	1501.0
70	1295.5
71	1090.0
72	911.5
73	733.0
74	653.5
75	441.5
76	309.0
77	225.0
78	141.0
79	122.5
80	104.0
81	75.5
82	47.0
83	29.0
84	11.0
85	9.5
86	8.0
87	8.0
88	8.0
89	4.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	335900.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.477820779994044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.27250137734852	20.195296219112834
2	9.37271613128099	4.963381958916345
3	3.2865222230967293	2.610598392378684
4	1.7270263888732726	1.8291158082762726
5	1.0647747332441335	1.409645727895207
6	0.7690664387951293	1.2217922000595416
7	0.5588099708789169	1.035724918130396
8	0.44412462474280123	0.94075617743376
9	0.40702054216935196	0.96993152724025
>10	4.732457077322659	27.85412325096755
>50	0.9309751627519985	17.337898183983327
>100	0.42838349880255	16.040190532896695
>500	0.0011243661385893704	0.20899077106281633
>1k	0.0033730984157681106	1.2372729979160466
>5k	0.0011243661385893704	2.145281333730277
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7206	2.145281333730277	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1658	0.4935992855016374	No Hit
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT	1450	0.4316760940756177	TruSeq Adapter, Index 13 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1048	0.3119976183387913	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	702	0.20899077106281633	No Hit
GAGCTAGCAAAAGCCGTAGCCTCTTCGTGTGTTGTTTCTGTTGCTGTTGCT	455	0.135456981244418	No Hit
ACATGTTACATGCTGTAGTTTAACATTTATAATTTTGTTCCCCTGTTTTGA	357	0.10628163143792796	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4927061625483775	0.0
2	0.0	0.0	0.0	0.6757963679666568	0.0
3	0.0	0.0	0.0	0.864543018755582	0.0
4	0.0	0.0	0.0	1.8088716880023816	0.0
5	0.0	0.0	0.0	1.9490919916641858	0.0
6	0.0	0.0	0.0	2.747841619529622	0.0
7	0.0	0.0	0.0	3.3426615064007144	0.0
8	0.0	0.0	0.0	3.85412325096755	0.0
9	0.0	0.0	0.0	4.587377195593927	0.0
10	0.0	0.0	0.0	5.864840726406669	0.0
11	0.0	0.0	0.0	6.857100327478416	0.0
12	0.0	0.0	0.0	7.447752307234296	0.0
13	0.0	0.0	0.0	7.643941649300387	0.0
14	0.0	0.0	0.0	7.875558201845787	0.0
15	0.0	0.0	0.0	8.098838940160762	0.0
16	0.0	0.0	0.0	8.573682643643942	0.0
17	0.0	0.0	0.0	9.127716582316166	0.0
18	0.0	0.0	0.0	9.935992855016375	0.0
19	0.0	0.0	0.0	10.259005656445371	0.0
20	0.0	0.0	0.0	10.581720750223282	0.0
21	0.0	0.0	0.0	10.925870794879428	0.0
22	0.0	0.0	0.0	11.340279845192022	0.0
23	0.0	0.0	0.0	11.726108961000298	0.0
24	0.0	0.0	0.0	12.007442691277166	0.0
25	0.0	0.0	0.0	12.258112533492111	0.0
26	0.0	0.0	0.0	12.485858886573386	0.0
27	0.0	0.0	0.0	12.76302470973504	0.0
28	0.0	0.0	0.0	13.041083655849956	0.0
29	0.0	0.0	0.0	13.338493599285501	0.0
30	0.0	0.0	0.0	13.69574278058946	0.0
31	0.0	0.0	0.0	13.966061327776124	0.0
32	0.0	0.0	0.0	14.234593629056267	0.0
33	0.0	0.0	0.0	14.510270913962488	0.0
34	0.0	0.0	0.0	14.766894909199166	0.0
35	0.0	0.0	0.0	15.080083358142303	0.0
36	0.0	0.0	0.0	15.363203334325693	0.0
37	0.0	0.0	0.0	15.653170586484073	0.0
38	0.0	0.0	0.0	15.923489133670735	0.0
39	0.0	0.0	0.0	16.211967847573682	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	20	7.0261495E-4	45.000004	26
CGGGTTA	40	6.7902874E-9	45.000004	6
TTTAGCG	40	6.7902874E-9	45.000004	1
CGGCGAA	20	7.0261495E-4	45.000004	17
CGAACGG	55	1.8189894E-12	45.000004	2
TCCGCGG	20	7.0261495E-4	45.000004	2
CGAAACG	20	7.0261495E-4	45.000004	1
ACGTTCC	20	7.0261495E-4	45.000004	27
GCGAACA	20	7.0261495E-4	45.000004	19
GCAACCG	20	7.0261495E-4	45.000004	1
TAGCGAG	20	7.0261495E-4	45.000004	1
TAAACGG	55	1.8189894E-12	45.000004	2
CCTACTC	20	7.0261495E-4	45.000004	19
ATTAACG	20	7.0261495E-4	45.000004	1
ATTCGAA	20	7.0261495E-4	45.000004	22
GCGTTGG	20	7.0261495E-4	45.000004	1
CATTCCG	20	7.0261495E-4	45.000004	1
CGTTCAT	20	7.0261495E-4	45.000004	17
TCGGCTA	40	6.7902874E-9	45.000004	11
GCCGCTC	20	7.0261495E-4	45.000004	20
>>END_MODULE