##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554198_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	422879
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24640618238314	33.0	31.0	34.0	30.0	34.0
2	32.434658613929756	34.0	31.0	34.0	30.0	34.0
3	32.53338188938207	34.0	31.0	34.0	31.0	34.0
4	36.068518417797996	37.0	35.0	37.0	35.0	37.0
5	35.89314437463199	37.0	35.0	37.0	35.0	37.0
6	35.865228587846644	37.0	35.0	37.0	35.0	37.0
7	35.927560838916094	37.0	35.0	37.0	35.0	37.0
8	36.049011655816436	37.0	35.0	37.0	35.0	37.0
9	37.70658982829604	39.0	38.0	39.0	35.0	39.0
10	37.26492448194401	39.0	37.0	39.0	34.0	39.0
11	37.246276121538315	39.0	37.0	39.0	34.0	39.0
12	37.14058394954585	39.0	37.0	39.0	34.0	39.0
13	37.10160589672223	39.0	37.0	39.0	33.0	39.0
14	38.315645846684276	40.0	38.0	41.0	34.0	41.0
15	38.32319174042693	40.0	38.0	41.0	33.0	41.0
16	38.309949181680814	40.0	38.0	41.0	34.0	41.0
17	38.232198808642664	40.0	38.0	41.0	33.0	41.0
18	38.14976388044807	40.0	37.0	41.0	33.0	41.0
19	38.102771714840415	40.0	37.0	41.0	34.0	41.0
20	38.046202341568154	40.0	37.0	41.0	33.0	41.0
21	37.95362976170488	40.0	37.0	41.0	33.0	41.0
22	37.945438293223354	40.0	37.0	41.0	33.0	41.0
23	37.82330406570201	40.0	36.0	41.0	33.0	41.0
24	37.79881242624959	40.0	36.0	41.0	33.0	41.0
25	37.74027322236385	40.0	36.0	41.0	33.0	41.0
26	37.65242776302441	40.0	36.0	41.0	33.0	41.0
27	37.57423518311385	40.0	36.0	41.0	33.0	41.0
28	37.51935660082435	40.0	36.0	41.0	33.0	41.0
29	37.408890013455384	39.0	36.0	41.0	32.0	41.0
30	37.3563028667775	39.0	36.0	41.0	32.0	41.0
31	37.278027520874765	39.0	35.0	41.0	32.0	41.0
32	37.2231654917837	39.0	35.0	41.0	32.0	41.0
33	37.130576358721996	39.0	35.0	41.0	32.0	41.0
34	36.96680374291464	39.0	35.0	41.0	31.0	41.0
35	36.99297907912192	39.0	35.0	41.0	31.0	41.0
36	36.82781362990359	39.0	35.0	41.0	31.0	41.0
37	36.7881403427458	39.0	35.0	41.0	31.0	41.0
38	36.692271311651794	39.0	35.0	41.0	31.0	41.0
39	36.64974614487832	39.0	35.0	41.0	31.0	41.0
40	36.574847651455855	39.0	35.0	41.0	30.0	41.0
41	36.48556206385278	39.0	35.0	41.0	30.0	41.0
42	36.347189148669	39.0	35.0	40.0	30.0	41.0
43	36.26453902889479	39.0	35.0	40.0	30.0	41.0
44	36.15342213730169	38.0	35.0	40.0	30.0	41.0
45	36.02190224626903	38.0	35.0	40.0	30.0	41.0
46	35.8896232728511	38.0	35.0	40.0	29.0	41.0
47	35.74776472702593	38.0	35.0	40.0	29.0	41.0
48	35.6591365378749	38.0	34.0	40.0	28.0	41.0
49	35.52561134508926	38.0	34.0	40.0	28.0	41.0
50	35.3722128552139	38.0	34.0	40.0	27.0	41.0
51	34.333407428602506	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	5.0
11	1.0
12	3.0
13	2.0
14	13.0
15	24.0
16	32.0
17	58.0
18	152.0
19	240.0
20	420.0
21	714.0
22	997.0
23	1331.0
24	1724.0
25	2177.0
26	2661.0
27	3327.0
28	4324.0
29	5451.0
30	6986.0
31	9179.0
32	12012.0
33	16548.0
34	25973.0
35	34042.0
36	36811.0
37	52783.0
38	86751.0
39	118098.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.267026738144956	33.16740722523464	21.650874127114374	24.91469190950603
2	23.35041465762074	32.58166047498221	20.429011608521584	23.638913258875473
3	21.04124347626626	31.872947107801526	20.543938100496835	26.541871315435383
4	20.05609169526035	32.562979008179646	21.032257454259966	26.348671842300043
5	19.00567301757713	34.411498324579846	17.566490650989998	29.016338006853026
6	19.756951752156056	38.170493214371014	19.60489880083901	22.46765623263392
7	85.04442169036533	7.043622407355296	4.477640176031442	3.4343157262479336
8	85.79026151688781	6.794615008075596	3.8526386980672953	3.562484776969298
9	77.67635659373012	11.022301887774045	7.327391523343557	3.9739499951522776
10	31.857339806422168	40.96561900685539	13.505754601197978	13.671286585524465
11	24.436304474802483	28.443833815346707	29.306018979424376	17.813842730426437
12	24.34951842016274	27.46766805634709	30.614667552656904	17.568145970833264
13	24.939994655681648	26.68612061606275	29.183052362496127	19.190832365759473
14	20.846625157550978	28.652404115598078	30.51109182532119	19.98987890152975
15	21.019014895513845	29.86244292102469	29.330139354283375	19.788402829178086
16	22.604338356834933	29.552425161807516	27.730627437162873	20.112609044194677
17	22.116491951598448	29.04755260961173	29.355915048985644	19.480040389804177
18	23.10022488702442	28.19955590133348	29.110691237919124	19.58952797372298
19	21.718269292161587	30.770267617923803	27.497463813525858	20.013999276388756
20	23.160998772698573	29.78582525970786	28.087467100518115	18.965708867075453
21	22.904187722729198	29.646778392873614	28.453292785879647	18.995741098517545
22	23.397000087495478	27.61026203713119	28.10047318500091	20.89226469037242
23	21.430243639433503	29.763596678955444	29.13717635541136	19.668983326199694
24	20.32188876723602	28.002572839984964	30.49241035851863	21.183128034260392
25	20.605184934697633	31.256931651843672	26.854726765812444	21.283156647646255
26	21.363321422912936	29.33628768512979	28.428226513967353	20.87216437798992
27	19.510545569772912	29.43560687572568	28.90093856635113	22.152908988150273
28	19.98964242726643	29.801432561087214	29.72859848798356	20.480326523662796
29	20.63427126908643	30.008347541495322	28.06973153076885	21.287649658649404
30	21.55746679310157	30.143137871589747	28.551193130895598	19.748202204413083
31	21.718032817898266	31.252911589367173	27.093092823242582	19.935962769491983
32	21.88971313307116	28.72003575490861	27.637220103150074	21.75303100887015
33	21.76367235071971	29.049917352244968	28.580752413811044	20.60565788322428
34	19.284949122562246	29.695492091118265	30.511328299584516	20.508230486734977
35	20.67683663648467	30.54750886187302	29.563775926447043	19.211878575195268
36	20.16747107328574	32.59277476535841	28.28681490449987	18.95293925685598
37	21.670737965233553	31.185989372846606	27.453006652021028	19.690266009898814
38	21.178871497520568	31.819267450027077	27.26122602446563	19.74063502798673
39	20.664776449055168	29.401791056070415	27.539556232397448	22.393876262476972
40	21.81971675112739	29.62880634886102	27.191702591048504	21.359774308963082
41	19.71675112739105	28.689530574939877	30.22448501817305	21.369233279496026
42	21.050702446799203	28.628283740739075	28.44336086682006	21.877652945641664
43	19.986095313316575	29.067652921994235	28.14563976929571	22.80061199539348
44	20.729333922942498	28.66517372581755	28.305969319829078	22.299523031410875
45	21.804582398274682	27.802988561739884	29.401318107543766	20.991110932441668
46	19.017023782216665	29.558573492653927	29.850619207858514	21.573783517270897
47	19.66945627472634	29.951120769771023	29.24524509375022	21.134177861752416
48	20.625994669870103	30.03908919572738	29.14521648036436	20.189699654038154
49	21.71850576642491	29.022959286226087	28.031422700110433	21.227112247238573
50	19.82600223704653	29.1225149510853	28.822665585191036	22.228817226677133
51	20.27932339983778	27.79329311694362	29.808290314723596	22.119093168495006
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6947.0
1	5232.5
2	3518.0
3	2196.5
4	875.0
5	777.0
6	679.0
7	742.5
8	806.0
9	938.0
10	1070.0
11	1203.0
12	1336.0
13	1508.5
14	1681.0
15	1570.5
16	1460.0
17	1454.0
18	1448.0
19	1907.0
20	2366.0
21	2026.5
22	1687.0
23	2331.5
24	2976.0
25	3462.0
26	4976.5
27	6005.0
28	7890.0
29	9775.0
30	10627.5
31	11480.0
32	13592.0
33	15704.0
34	15965.5
35	16227.0
36	19408.5
37	22590.0
38	20878.0
39	19166.0
40	21371.5
41	23577.0
42	23295.0
43	23013.0
44	24585.0
45	26157.0
46	27373.0
47	28589.0
48	29421.0
49	30253.0
50	30234.5
51	30216.0
52	28151.0
53	26086.0
54	23507.0
55	20928.0
56	18762.5
57	16597.0
58	16223.5
59	15850.0
60	14021.5
61	12193.0
62	11308.5
63	10424.0
64	9729.5
65	9035.0
66	7376.0
67	5717.0
68	4792.0
69	3867.0
70	3506.0
71	3145.0
72	2582.0
73	2019.0
74	1867.5
75	1330.0
76	944.0
77	628.5
78	313.0
79	283.5
80	254.0
81	167.5
82	81.0
83	78.5
84	76.0
85	65.0
86	54.0
87	36.0
88	18.0
89	11.0
90	4.0
91	4.0
92	4.0
93	3.0
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	422879.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.11733692003748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.25786158063018	17.90901866701565
2	8.6184890663175	4.1571000910807685
3	3.1500733878862484	2.279141436554897
4	1.8257506366769392	1.7612897294684273
5	1.267050475255515	1.5278941603215432
6	0.981258770629925	1.4199209026214208
7	0.7764130805219911	1.3107511096451134
8	0.6425142670464902	1.2396586443432909
9	0.5395920724555624	1.1712171429712865
>10	6.578027057983195	34.82322573657506
>50	0.9472838600860862	16.004465359086797
>100	0.41068952161960065	14.06087147974399
>500	0.00199848915629976	0.3477502750020667
>1k	0.00199848915629976	0.522227890457019
>5k	9.9924457814988E-4	1.4654673751126592
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6081	1.4379999952705147	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1092	0.25822989554931786	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1075	0.2542098330728175	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT	901	0.2130633112545196	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	542	0.12816905072136475	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.258702844075965	0.0
2	0.0	0.0	0.0	0.34714421855897315	0.0
3	0.0	0.0	0.0	0.44882815178810015	0.0
4	0.0	0.0	0.0	0.9553560238271468	0.0
5	0.0	0.0	0.0	1.0234606116643294	0.0
6	0.0	0.0	0.0	1.5117799654274626	0.0
7	0.0	0.0	0.0	1.8681466802560542	0.0
8	0.0	0.0	0.0	2.2448501817304716	0.0
9	0.0	0.0	0.0	2.7326965869669575	0.0
10	0.0	0.0	0.0	3.53529023668709	0.0
11	0.0	0.0	0.0	4.189378049040033	0.0
12	0.0	0.0	0.0	4.521151440482975	0.0
13	0.0	0.0	0.0	4.669420803586841	0.0
14	0.0	0.0	0.0	4.825493817380385	0.0
15	0.0	0.0	0.0	4.949879279888573	0.0
16	0.0	0.0	0.0	5.199359627694919	0.0
17	0.0	0.0	0.0	5.537044875720951	0.0
18	0.0	0.0	0.0	5.953712527697048	0.0
19	0.0	0.0	0.0	6.149986166255595	0.0
20	0.0	0.0	0.0	6.368488385566557	0.0
21	0.0	0.0	0.0	6.595503678357166	0.0
22	0.0	0.0	0.0	6.874543309078956	0.0
23	0.0	0.0	0.0	7.144360443531128	0.0
24	0.0	0.0	0.0	7.340161133563028	0.0
25	0.0	0.0	0.0	7.509949654629338	0.0
26	0.0	0.0	0.0	7.665313245632912	0.0
27	0.0	0.0	0.0	7.872701174567666	0.0
28	0.0	0.0	0.0	8.064481802123066	0.0
29	0.0	0.0	0.0	8.261701337734907	0.0
30	0.0	0.0	0.0	8.5608412808392	0.0
31	2.3647426332355117E-4	0.0	0.0	8.750966588551336	0.0
32	2.3647426332355117E-4	0.0	0.0	8.949368495479794	0.0
33	2.3647426332355117E-4	0.0	0.0	9.16148590968102	0.0
34	2.3647426332355117E-4	0.0	0.0	9.35988781660948	0.0
35	2.3647426332355117E-4	0.0	0.0	9.582410098396942	0.0
36	2.3647426332355117E-4	0.0	0.0	9.79736520375805	0.0
37	2.3647426332355117E-4	0.0	0.0	9.984416346046977	0.0
38	2.3647426332355117E-4	0.0	0.0	10.212377535890882	0.0
39	2.3647426332355117E-4	0.0	0.0	10.464459100593787	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.028224E-4	45.0	1
ACAACGT	20	7.028224E-4	45.0	31
TATCACG	20	7.028224E-4	45.0	1
AAATCCG	20	7.028224E-4	45.0	1
AGTTTCG	20	7.028224E-4	45.0	34
CGACGCT	20	7.028224E-4	45.0	18
TAGGTAC	20	7.028224E-4	45.0	30
GACCGTA	35	1.2095006E-7	45.0	9
GACCGAA	25	3.88661E-5	45.0	28
CGACCAG	20	7.028224E-4	45.0	1
TCCTCGA	20	7.028224E-4	45.0	44
CACGATT	20	7.028224E-4	45.0	41
ACGGGCC	85	0.0	45.0	5
CGTTATC	20	7.028224E-4	45.0	44
GTTACGG	35	1.2095006E-7	45.0	2
CCGTATA	20	7.028224E-4	45.0	31
ACGATGG	20	7.028224E-4	45.0	2
ACTAGGT	20	7.028224E-4	45.0	19
TACGCGG	55	1.8189894E-12	45.0	2
CGGATTA	20	7.028224E-4	45.0	6
>>END_MODULE