##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554197_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	411320
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32410045706506	34.0	31.0	34.0	30.0	34.0
2	32.50121316736361	34.0	31.0	34.0	30.0	34.0
3	32.59440824662064	34.0	31.0	34.0	31.0	34.0
4	36.10563794612467	37.0	35.0	37.0	35.0	37.0
5	35.946270543615675	37.0	35.0	37.0	35.0	37.0
6	35.93052124866284	37.0	35.0	37.0	35.0	37.0
7	35.95613877273169	37.0	35.0	37.0	35.0	37.0
8	36.07830885928231	37.0	35.0	37.0	35.0	37.0
9	37.74119906642031	39.0	38.0	39.0	35.0	39.0
10	37.322991831177674	39.0	37.0	39.0	34.0	39.0
11	37.31522658757172	39.0	37.0	39.0	34.0	39.0
12	37.232942720995815	39.0	37.0	39.0	34.0	39.0
13	37.194063016629386	39.0	37.0	39.0	34.0	39.0
14	38.41897549353302	40.0	38.0	41.0	34.0	41.0
15	38.430560147816784	40.0	38.0	41.0	34.0	41.0
16	38.41514878926383	40.0	38.0	41.0	34.0	41.0
17	38.344386365846546	40.0	38.0	41.0	34.0	41.0
18	38.260979286200524	40.0	37.0	41.0	34.0	41.0
19	38.216928425556745	40.0	37.0	41.0	34.0	41.0
20	38.15906836526305	40.0	37.0	41.0	33.0	41.0
21	38.071550131284646	40.0	37.0	41.0	33.0	41.0
22	38.0624258484878	40.0	37.0	41.0	33.0	41.0
23	37.94243168336089	40.0	37.0	41.0	33.0	41.0
24	37.942548380822714	40.0	37.0	41.0	33.0	41.0
25	37.89052805601478	40.0	37.0	41.0	33.0	41.0
26	37.83257317903335	40.0	36.0	41.0	33.0	41.0
27	37.781109598366236	40.0	36.0	41.0	33.0	41.0
28	37.676242341729065	40.0	36.0	41.0	33.0	41.0
29	37.5998298162015	40.0	36.0	41.0	33.0	41.0
30	37.541296314305164	40.0	36.0	41.0	33.0	41.0
31	37.42054604687348	40.0	36.0	41.0	33.0	41.0
32	37.382500243119715	40.0	36.0	41.0	33.0	41.0
33	37.30682193912283	39.0	35.0	41.0	32.0	41.0
34	37.142297967519205	39.0	35.0	41.0	31.0	41.0
35	37.1337790528056	39.0	35.0	41.0	32.0	41.0
36	37.00594913935622	39.0	35.0	41.0	31.0	41.0
37	36.96523144996596	39.0	35.0	41.0	31.0	41.0
38	36.8756685792084	39.0	35.0	41.0	31.0	41.0
39	36.83061363415346	39.0	35.0	41.0	31.0	41.0
40	36.72873674997569	39.0	35.0	41.0	31.0	41.0
41	36.65406009919284	39.0	35.0	41.0	31.0	41.0
42	36.52170572790042	39.0	35.0	41.0	30.0	41.0
43	36.45168481960518	39.0	35.0	41.0	30.0	41.0
44	36.35583487309151	39.0	35.0	40.0	30.0	41.0
45	36.220823203345326	38.0	35.0	40.0	30.0	41.0
46	36.045499854128174	38.0	35.0	40.0	30.0	41.0
47	35.93839832733638	38.0	35.0	40.0	29.0	41.0
48	35.84191383837401	38.0	35.0	40.0	29.0	41.0
49	35.752163765438105	38.0	35.0	40.0	29.0	41.0
50	35.61943012739473	38.0	34.0	40.0	28.0	41.0
51	34.61640571817563	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	1.0
11	1.0
12	4.0
13	7.0
14	13.0
15	20.0
16	24.0
17	70.0
18	120.0
19	230.0
20	370.0
21	594.0
22	873.0
23	1226.0
24	1514.0
25	1958.0
26	2362.0
27	3025.0
28	3806.0
29	4921.0
30	6400.0
31	8195.0
32	11024.0
33	15379.0
34	24348.0
35	32122.0
36	34570.0
37	50872.0
38	84348.0
39	122867.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.25551881746572	33.09953321015268	21.559612953418263	25.085335018963338
2	23.574589127686473	32.272439949431096	20.54434503549548	23.60862588738695
3	21.102547894583292	31.855732762812412	20.460225615092874	26.58149372751143
4	20.10916075075367	32.57196343479529	20.9265292229894	26.392346591461635
5	19.103617621316737	34.40095302927161	17.42268793153749	29.07274141787416
6	19.67373334629972	38.15107458912769	19.619274530778956	22.55591753379364
7	84.85850432753088	7.079402897986969	4.602013031216571	3.4600797432655837
8	85.68340951084313	6.776475736652728	3.8408052124866283	3.699309540017505
9	77.44238062822134	11.133667217738013	7.346105222211417	4.0778469318292325
10	32.064086356121756	40.96056598268988	13.349217154526889	13.626130506661479
11	24.3562190022367	28.44816687737042	29.23538850530001	17.960225615092874
12	24.287173003987164	27.415880579597395	30.596858893319066	17.70008752309637
13	24.797481279782165	26.701108625887386	29.279393173198482	19.222016921131964
14	20.752455509092677	28.615433239327043	30.673198482932996	19.958912768647284
15	20.844111640571818	29.907614509384423	29.346737333462997	19.901536516580766
16	22.48516969755908	29.630701157249828	27.710055431294368	20.174073713896725
17	22.086696489351358	28.98303024409219	29.54366430030147	19.386608966254983
18	23.067927647573665	28.130166293883107	29.144704852669456	19.657201205873772
19	21.615773606924048	30.815180394826413	27.571234075658857	19.99781192259068
20	23.117767188563647	29.67324710687543	28.12141398424584	19.087571720315083
21	22.654381017212877	29.762228921520954	28.521102791014297	19.062287270251872
22	23.24978119225907	27.595789166585625	28.27773995915589	20.876689681999416
23	21.642273655547992	29.79310512496353	28.968443061363413	19.59617815812506
24	20.125692891179618	28.00155596615774	30.6080424000778	21.264708742584848
25	20.389477778858307	31.428571428571427	26.970728386657587	21.21122240591267
26	21.247690362734613	29.379315374890595	28.445006321112515	20.927987941262277
27	19.504278906933774	29.56189827871244	28.780754643586505	22.153068170767288
28	20.06710104055237	29.751045414762228	29.80744918798016	20.37440435670524
29	20.61047359719926	30.124477292618884	28.06817076728581	21.196878342896042
30	21.427598949722842	30.13468832052903	28.670864533696395	19.766848196051736
31	21.730282991344936	31.14679568219391	27.134834192356315	19.988087134104834
32	21.707186618691043	28.841777691335214	27.708353593309344	21.7426820966644
33	21.83604006612856	29.036759700476516	28.464212778372072	20.66298745502285
34	19.167558105611203	29.93751823397841	30.372459399008072	20.522464261402316
35	20.722551784498688	30.630409413595256	29.4960128367208	19.151025965185255
36	20.088495575221238	32.673587474472434	28.3224739861908	18.91544296411553
37	21.607507536711076	31.179373723621513	27.50072935913644	19.712389380530972
38	21.190800350092385	31.79543907420014	27.332733638043372	19.681026937664107
39	20.70018477098123	29.27039774384907	27.636633278226196	22.3927842069435
40	21.7978702713216	29.558251483030247	27.247884858504328	21.39599338714383
41	19.667412233783914	28.773704171934263	30.324078576290965	21.23480501799086
42	21.00068073519401	28.652387435573278	28.439657687445298	21.90727414178742
43	20.164105805698725	28.831809783137217	28.1338130895653	22.870271321598757
44	20.686326947388896	28.635369055723036	28.375474083438686	22.302829913449383
45	21.755081201983856	27.879023631236024	29.47267334435476	20.89322182242536
46	19.07541573470777	29.467810950111833	29.924876008946804	21.53189730623359
47	19.48750364679568	30.004862394242927	29.305163862685983	21.202470096275405
48	20.68997374307109	30.04497714674706	29.132305747349996	20.13274336283186
49	21.925994359622678	28.74696100359817	28.09953321015268	21.22751142662647
50	19.82179325099679	28.90571817562968	28.875328211611397	22.39716036176213
51	20.139550714771953	27.862491490810076	29.97641738792181	22.02154040649616
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6649.0
1	5099.5
2	3550.0
3	2202.0
4	854.0
5	779.5
6	705.0
7	755.0
8	805.0
9	945.0
10	1085.0
11	1172.5
12	1260.0
13	1442.0
14	1624.0
15	1560.5
16	1497.0
17	1429.0
18	1361.0
19	1855.0
20	2349.0
21	1955.5
22	1562.0
23	2212.0
24	2862.0
25	3336.5
26	4854.0
27	5897.0
28	7739.0
29	9581.0
30	10371.5
31	11162.0
32	13193.0
33	15224.0
34	15637.0
35	16050.0
36	18997.5
37	21945.0
38	20288.0
39	18631.0
40	20780.0
41	22929.0
42	22533.0
43	22137.0
44	23921.0
45	25705.0
46	27014.5
47	28324.0
48	28579.5
49	28835.0
50	29017.0
51	29199.0
52	27116.0
53	25033.0
54	22627.5
55	20222.0
56	18319.5
57	16417.0
58	15983.5
59	15550.0
60	13759.0
61	11968.0
62	10996.5
63	10025.0
64	9379.5
65	8734.0
66	7193.0
67	5652.0
68	4756.0
69	3860.0
70	3488.0
71	3116.0
72	2530.5
73	1945.0
74	1757.0
75	1228.0
76	887.0
77	602.0
78	317.0
79	276.0
80	235.0
81	142.0
82	49.0
83	52.0
84	55.0
85	52.0
86	49.0
87	35.5
88	22.0
89	16.5
90	11.0
91	8.0
92	5.0
93	3.5
94	2.0
95	2.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	411320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.512670715536014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.54426565256782	18.272790376728363
2	8.473956325413495	4.154386021253891
3	3.1877590582819906	2.3442146434840088
4	1.8427398016513208	1.806818958891639
5	1.2018270748248814	1.472999567109909
6	0.9288662479394743	1.366139548470946
7	0.7488921599416551	1.2850142842667425
8	0.6289094372722382	1.2332999956597943
9	0.5459213875168002	1.204379208789107
>10	6.587051466958853	35.232626048230614
>50	0.9128685472576543	15.640263498095951
>100	0.39194356026834665	13.64497429607133
>500	0.001999712042185442	0.36175492718196045
>1k	0.001999712042185442	0.5431225736011005
>5k	9.99856021092721E-4	1.437216052164645
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5864	1.4256539920256734	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1147	0.2788583098317612	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1069	0.2598949722843528	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT	962	0.23388116308470291	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	514	0.12496353204317805	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2718078381795196	0.0
2	0.0	0.0	0.0	0.36030341340075855	0.0
3	0.0	0.0	0.0	0.47019352329086844	0.0
4	0.0	0.0	0.0	0.9997082563454245	0.0
5	0.0	0.0	0.0	1.0753184868229115	0.0
6	0.0	0.0	0.0	1.580764368374988	0.0
7	0.0	0.0	0.0	1.9315861130020422	0.0
8	0.0	0.0	0.0	2.312554701935233	0.0
9	0.0	0.0	0.0	2.802197802197802	0.0
10	0.0	0.0	0.0	3.595254303218905	0.0
11	0.0	0.0	0.0	4.244870174073714	0.0
12	0.0	0.0	0.0	4.59496255956433	0.0
13	0.0	0.0	0.0	4.736458232033454	0.0
14	0.0	0.0	0.0	4.911747544490908	0.0
15	0.0	0.0	0.0	5.049110181853544	0.0
16	0.0	0.0	0.0	5.309248273850044	0.0
17	0.0	0.0	0.0	5.639891082368958	0.0
18	0.0	0.0	0.0	6.073616648837888	0.0
19	0.0	0.0	0.0	6.275892249343577	0.0
20	0.0	0.0	0.0	6.494456870563066	0.0
21	0.0	0.0	0.0	6.737576582709326	0.0
22	0.0	0.0	0.0	7.009627540600992	0.0
23	0.0	0.0	0.0	7.265632597491004	0.0
24	0.0	0.0	0.0	7.455995332101526	0.0
25	0.0	0.0	0.0	7.629582806573957	0.0
26	0.0	0.0	0.0	7.786881260332588	0.0
27	0.0	0.0	0.0	7.9945054945054945	0.0
28	0.0	0.0	0.0	8.183895750267432	0.0
29	0.0	0.0	0.0	8.374987844014393	0.0
30	0.0	0.0	0.0	8.657736069240494	0.0
31	0.0	0.0	0.0	8.846396965865992	0.0
32	0.0	0.0	0.0	9.049645045220267	0.0
33	0.0	0.0	0.0	9.253865603423126	0.0
34	0.0	0.0	0.0	9.437907225517845	0.0
35	0.0	0.0	0.0	9.658903043858796	0.0
36	0.0	0.0	0.0	9.870417193426043	0.0
37	0.0	0.0	0.0	10.069775357385977	0.0
38	0.0	0.0	0.0	10.322619858018088	0.0
39	0.0	0.0	0.0	10.58056987260527	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	20	7.0279976E-4	45.000004	1
ATTTACG	20	7.0279976E-4	45.000004	1
AACGTTG	20	7.0279976E-4	45.000004	17
CTTCGAC	20	7.0279976E-4	45.000004	34
CGAACCG	20	7.0279976E-4	45.000004	1
TTGGACC	45	3.8380676E-10	45.000004	27
CGAGTAT	20	7.0279976E-4	45.000004	24
CGACGTA	20	7.0279976E-4	45.000004	16
CATGCGA	35	1.2093915E-7	45.000004	45
CGACGAG	20	7.0279976E-4	45.000004	39
TCCGATC	20	7.0279976E-4	45.000004	15
TCATTCG	20	7.0279976E-4	45.000004	33
TCTTCGA	20	7.0279976E-4	45.000004	33
CGTTGCG	20	7.0279976E-4	45.000004	1
TAACCCG	20	7.0279976E-4	45.000004	1
CAAATCG	20	7.0279976E-4	45.000004	14
AACTCGA	40	6.7975634E-9	45.000004	12
ACTCCCG	35	1.2093915E-7	45.000004	1
TGAATCG	20	7.0279976E-4	45.000004	17
CGGTAAG	20	7.0279976E-4	45.000004	1
>>END_MODULE