##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554196_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	418522
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26472682439633	34.0	31.0	34.0	30.0	34.0
2	32.45807149922824	34.0	31.0	34.0	30.0	34.0
3	32.54988985047381	34.0	31.0	34.0	31.0	34.0
4	36.0757092817104	37.0	35.0	37.0	35.0	37.0
5	35.90114020290451	37.0	35.0	37.0	35.0	37.0
6	35.877722079126066	37.0	35.0	37.0	35.0	37.0
7	35.94528364100334	37.0	35.0	37.0	35.0	37.0
8	36.071403653810314	37.0	35.0	37.0	35.0	37.0
9	37.73784890638963	39.0	38.0	39.0	35.0	39.0
10	37.25880359933289	39.0	37.0	39.0	34.0	39.0
11	37.15915053449998	39.0	37.0	39.0	34.0	39.0
12	37.175257214674495	39.0	37.0	39.0	34.0	39.0
13	37.16321483697392	39.0	37.0	39.0	33.0	39.0
14	38.41290780412977	40.0	38.0	41.0	34.0	41.0
15	38.43501177954803	40.0	38.0	41.0	34.0	41.0
16	38.40192152383865	40.0	38.0	41.0	34.0	41.0
17	38.35881984698534	40.0	38.0	41.0	34.0	41.0
18	38.239435441864465	40.0	37.0	41.0	34.0	41.0
19	38.21149425836634	40.0	37.0	41.0	34.0	41.0
20	38.14271173319443	40.0	37.0	41.0	33.0	41.0
21	38.07318850621951	40.0	37.0	41.0	33.0	41.0
22	38.02860781512083	40.0	37.0	41.0	33.0	41.0
23	37.926376630141306	40.0	37.0	41.0	33.0	41.0
24	37.859961961378374	40.0	37.0	41.0	33.0	41.0
25	37.819481413163466	40.0	37.0	41.0	33.0	41.0
26	37.69894533620694	40.0	36.0	41.0	33.0	41.0
27	37.62250252077549	40.0	36.0	41.0	33.0	41.0
28	37.60227897219262	40.0	36.0	41.0	33.0	41.0
29	37.47759018641792	40.0	36.0	41.0	32.0	41.0
30	37.439924305054454	40.0	36.0	41.0	32.0	41.0
31	37.35504465715064	40.0	36.0	41.0	32.0	41.0
32	37.30501383439819	39.0	36.0	41.0	32.0	41.0
33	37.215625462938625	39.0	36.0	41.0	32.0	41.0
34	37.09051376032801	39.0	35.0	41.0	31.0	41.0
35	37.05272602157115	39.0	35.0	41.0	31.0	41.0
36	36.94113332154582	39.0	35.0	41.0	31.0	41.0
37	36.888177921351804	39.0	35.0	41.0	31.0	41.0
38	36.842464673302715	39.0	35.0	41.0	31.0	41.0
39	36.77004554121408	39.0	35.0	41.0	31.0	41.0
40	36.69369591084818	39.0	35.0	41.0	31.0	41.0
41	36.61957555397327	39.0	35.0	41.0	30.0	41.0
42	36.51379616842125	39.0	35.0	41.0	30.0	41.0
43	36.43046721558245	39.0	35.0	41.0	30.0	41.0
44	36.33515800842011	39.0	35.0	40.0	30.0	41.0
45	36.18424360009749	39.0	35.0	40.0	30.0	41.0
46	36.08110445806911	38.0	35.0	40.0	29.0	41.0
47	35.9640090604556	38.0	35.0	40.0	29.0	41.0
48	35.86294627283632	38.0	35.0	40.0	28.0	41.0
49	35.751074017614364	38.0	35.0	40.0	28.0	41.0
50	35.61100730666488	38.0	34.0	40.0	28.0	41.0
51	34.62332207148011	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	4.0
13	8.0
14	18.0
15	26.0
16	51.0
17	80.0
18	123.0
19	235.0
20	330.0
21	619.0
22	879.0
23	1373.0
24	1763.0
25	2281.0
26	2687.0
27	3311.0
28	4210.0
29	5245.0
30	6949.0
31	8804.0
32	11447.0
33	15358.0
34	22901.0
35	32511.0
36	35767.0
37	50215.0
38	84460.0
39	126813.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.665499065759985	33.967390005782256	22.176134110034837	26.19097681842293
2	22.628440082002857	33.89642599433243	19.91293169773632	23.562202225928385
3	20.368821710686653	33.11725548477739	20.67155370565944	25.84236909887652
4	20.255566015645535	33.957354691031775	19.355254920888267	26.431824372434427
5	18.344555363875735	35.9237985099947	16.543216366164742	29.18842975996483
6	19.323715360243906	39.01395864494578	19.457758492982446	22.20456750182786
7	86.03824888536326	6.801315104104444	3.753924524875634	3.406511485656668
8	87.17749604560811	6.569547120581475	3.315476844705893	2.9374799891045154
9	79.12104978949732	11.099535986160824	5.927048040485327	3.8523661838565237
10	32.14813080315969	39.946765044609364	14.170103363741928	13.735000788489016
11	21.14966477270012	30.799336713482205	31.57635679844787	16.4746417153698
12	23.664466861957077	27.904148407968997	30.913070280654303	17.518314449419627
13	22.742890457371416	28.862759902705232	29.68422209585159	18.71012754407176
14	19.984851453448087	30.072015330137962	29.379578612354905	20.563554604059046
15	18.55505803757031	32.76267436359379	30.55323256602998	18.12903503280592
16	19.777693884670338	33.403023019100544	28.522992817581873	18.29629027864724
17	19.675907120772624	32.11778592284277	29.795566302368815	18.410740654015797
18	20.258433248431384	31.622949331217953	29.041723015755444	19.07689440459522
19	19.869445333817577	32.10536124743741	29.34230458613884	18.68288883260617
20	22.075780962530047	30.736257592193482	29.25007526486063	17.937886180415845
21	21.063886725190073	31.913973458981847	29.343021394335306	17.679118421492777
22	20.714323261381722	29.813486507280384	30.289447149731675	19.182743081606223
23	19.195406693077068	31.634179326295868	29.523657059843927	19.646756920783137
24	18.8518166309059	32.18970567855453	30.184793153048105	18.77368453749146
25	19.186566058654027	33.159308232303204	28.606859376568018	19.04726633247476
26	20.123434371430893	32.8028156225957	27.908210321082283	19.165539684891115
27	18.120911206579343	32.54691509645849	29.61206340407434	19.72011029288783
28	19.722738589608195	32.14096272119506	29.351145220561882	18.785153468634864
29	19.152159265223812	31.947902380281086	28.96215730594807	19.93778104854703
30	18.965072325947023	32.54237531121423	28.069253229220926	20.423299133617824
31	19.773393035491562	33.67182609277409	27.49198369500289	19.06279717673145
32	19.85606491415027	32.104883375306436	27.813113767018223	20.22593794352507
33	19.54592590114737	32.42314621453591	28.395400958611493	19.63552692570522
34	18.717295626036385	31.903460272100393	29.06800598295908	20.311238118904143
35	19.365529171704235	32.8298153979958	28.610204481484846	19.194450948815117
36	19.286202397962356	34.14659205489795	27.712521683447942	18.854683863691754
37	18.34097132289342	34.14611418276697	27.1854287229823	20.327485771357303
38	18.573456114612853	32.73854182097954	29.871786907259356	18.81621515714825
39	19.046310588212805	32.7220552324609	28.080483224298842	20.151150955027454
40	19.1927783963567	33.11510506018799	27.536664739249073	20.15545180420623
41	17.925222568945003	32.29674903589297	29.88301690233727	19.89501149282475
42	20.085204600952878	32.08027296056121	27.367498004883856	20.467024433602056
43	19.410210215950414	32.902929834035014	28.15718170132036	19.529678248694214
44	19.989869110823324	31.270040762492773	27.658761068713233	21.08132905797067
45	19.4606257257683	32.715365022627246	27.766043362117166	20.057965889487292
46	17.932151714844142	32.843195817663116	28.61378852246716	20.61086394502559
47	18.63127864246085	31.699647808239472	29.69855825978085	19.97051528951883
48	18.794949847319852	32.353137947348046	29.186757207506414	19.66515499782568
49	19.63839415849107	32.798036901285954	27.852538217823675	19.7110307223993
50	18.661384586712288	31.035883418314924	29.317933107459105	20.984798887513676
51	19.260636238955183	29.551612579505974	30.24596078581293	20.941790395725913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7717.0
1	5700.5
2	3684.0
3	2349.5
4	1015.0
5	919.5
6	824.0
7	865.0
8	906.0
9	1240.5
10	1575.0
11	1691.0
12	1807.0
13	2119.0
14	2431.0
15	2214.5
16	1998.0
17	1853.5
18	1709.0
19	1867.0
20	2025.0
21	2126.5
22	2228.0
23	2602.0
24	2976.0
25	3597.5
26	5117.5
27	6016.0
28	8038.5
29	10061.0
30	11775.5
31	13490.0
32	15666.5
33	17843.0
34	19471.5
35	21100.0
36	23382.5
37	25665.0
38	24645.5
39	23626.0
40	25204.0
41	26782.0
42	27348.0
43	27914.0
44	29109.0
45	30304.0
46	30003.5
47	29703.0
48	30072.5
49	30442.0
50	29645.5
51	28849.0
52	25445.5
53	22042.0
54	19654.0
55	17266.0
56	15113.5
57	12961.0
58	11730.5
59	10500.0
60	9782.5
61	9065.0
62	7761.5
63	6458.0
64	5685.0
65	4912.0
66	3847.5
67	2783.0
68	2444.0
69	2105.0
70	1703.0
71	1301.0
72	1069.0
73	837.0
74	690.0
75	417.0
76	291.0
77	272.5
78	254.0
79	191.5
80	129.0
81	81.0
82	33.0
83	29.0
84	25.0
85	27.5
86	30.0
87	31.0
88	32.0
89	24.5
90	17.0
91	12.5
92	8.0
93	7.0
94	6.0
95	5.0
96	4.0
97	4.5
98	5.0
99	5.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	418522.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.2054751285394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.85628805269036	18.119320186741774
2	8.54656541383422	4.137473531179985
3	3.331673078763194	2.4193418953328036
4	1.8673872605740482	1.8080398356470584
5	1.2455733625901335	1.5074847524473334
6	0.9256820985754994	1.3443945008402043
7	0.7427448817137712	1.2584944932820838
8	0.5778015344762008	1.1188768537596463
9	0.4998283169105649	1.0888723704166552
>10	5.955954224218932	31.772811825014948
>50	0.9816628143978785	16.735968333057276
>100	0.4618413648185538	15.876001132078665
>500	0.003998626535225574	0.63759526577902
>1k	0.001999313267612787	0.5693592639005822
>5k	9.996566338063935E-4	1.6059657605219566
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6637	1.58581866664118	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1328	0.31730709496752857	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1025	0.24490946712478673	No Hit
CTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGCT	876	0.20930799336713485	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	637	0.1522022737155992	No Hit
GAGCACGGGGTTTCACAAGTGAGAGGTTGGTTGAAAGATAAATTTAGAGAT	590	0.14097227863768214	No Hit
AGCACTGGGGATTTTTTTTTAAAGCAAAACTATTTATTGACTTTCTTCATC	532	0.1271139868394015	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3893606548759682E-4	0.0	0.0	0.2556615900717286	0.0
2	2.3893606548759682E-4	0.0	0.0	0.3395281490578751	0.0
3	2.3893606548759682E-4	0.0	0.0	0.43414683099096346	0.0
4	2.3893606548759682E-4	0.0	0.0	0.9084349209838432	0.0
5	2.3893606548759682E-4	0.0	0.0	0.9653017045698912	0.0
6	2.3893606548759682E-4	0.0	0.0	1.3700593995058803	0.0
7	2.3893606548759682E-4	0.0	0.0	1.7052866993849787	0.0
8	2.3893606548759682E-4	0.0	0.0	2.0319123009065234	0.0
9	2.3893606548759682E-4	0.0	0.0	2.5090676236852545	0.0
10	2.3893606548759682E-4	0.0	0.0	3.197442428355021	0.0
11	2.3893606548759682E-4	0.0	0.0	3.883427872369911	0.0
12	2.3893606548759682E-4	0.0	0.0	4.179469657509044	0.0
13	2.3893606548759682E-4	0.0	0.0	4.287707695174925	0.0
14	2.3893606548759682E-4	0.0	0.0	4.412671257424938	0.0
15	2.3893606548759682E-4	0.0	0.0	4.545280773770554	0.0
16	2.3893606548759682E-4	0.0	0.0	4.804287468759109	0.0
17	2.3893606548759682E-4	0.0	0.0	5.129957326018704	0.0
18	2.3893606548759682E-4	0.0	0.0	5.5397326783299325	0.0
19	2.3893606548759682E-4	0.0	0.0	5.738288548750125	0.0
20	2.3893606548759682E-4	0.0	0.0	5.952853135557987	0.0
21	2.3893606548759682E-4	0.0	0.0	6.190594520718146	0.0
22	2.3893606548759682E-4	0.0	0.0	6.459397594391692	0.0
23	2.3893606548759682E-4	0.0	0.0	6.731545772982066	0.0
24	2.3893606548759682E-4	0.0	0.0	6.923411433568606	0.0
25	2.3893606548759682E-4	0.0	0.0	7.1083479482560055	0.0
26	2.3893606548759682E-4	0.0	0.0	7.2815765957345135	0.0
27	2.3893606548759682E-4	0.0	0.0	7.452654818623633	0.0
28	2.3893606548759682E-4	0.0	0.0	7.641414310358834	0.0
29	2.3893606548759682E-4	0.0	0.0	7.850722303725969	0.0
30	2.3893606548759682E-4	0.0	0.0	8.102321980684408	0.0
31	2.3893606548759682E-4	0.0	0.0	8.292515088812536	0.0
32	2.3893606548759682E-4	0.0	0.0	8.497044360869918	0.0
33	2.3893606548759682E-4	0.0	0.0	8.710414267350343	0.0
34	2.3893606548759682E-4	0.0	0.0	8.902279927936883	0.0
35	2.3893606548759682E-4	0.0	0.0	9.127357701626199	0.0
36	2.3893606548759682E-4	0.0	0.0	9.32806399663578	0.0
37	2.3893606548759682E-4	0.0	0.0	9.551230281801196	0.0
38	2.3893606548759682E-4	0.0	0.0	9.768423165329422	0.0
39	2.3893606548759682E-4	0.0	0.0	9.992306258691299	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAC	20	7.028139E-4	45.000004	40
TCGCATG	20	7.028139E-4	45.000004	1
ACCGGTA	40	6.7975634E-9	45.000004	43
CGAACCG	20	7.028139E-4	45.000004	1
CGAGTTA	20	7.028139E-4	45.000004	27
GTCGAGT	20	7.028139E-4	45.000004	25
GATAACG	40	6.7975634E-9	45.000004	1
TTAGCGA	20	7.028139E-4	45.000004	38
GCGCGAC	20	7.028139E-4	45.000004	9
CGGTTAG	20	7.028139E-4	45.000004	1
TAGCGAT	20	7.028139E-4	45.000004	39
GGCAACG	20	7.028139E-4	45.000004	1
CGACCCG	20	7.028139E-4	45.000004	32
ACTTCCG	20	7.028139E-4	45.000004	1
GATTTCG	20	7.028139E-4	45.000004	9
AAGCGAC	20	7.028139E-4	45.000004	36
CGCGAAG	20	7.028139E-4	45.000004	1
TCGATAG	20	7.028139E-4	45.000004	44
CCTCGCG	20	7.028139E-4	45.000004	1
ATCGTGT	20	7.028139E-4	45.000004	8
>>END_MODULE