##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554194_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	492357
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.981084863219166	33.0	31.0	34.0	30.0	34.0
2	32.176101893544725	34.0	31.0	34.0	30.0	34.0
3	32.291818741279194	34.0	31.0	34.0	30.0	34.0
4	35.8701755027348	37.0	35.0	37.0	35.0	37.0
5	35.669122201979455	37.0	35.0	37.0	33.0	37.0
6	35.63995027997977	37.0	35.0	37.0	33.0	37.0
7	35.81091768777534	37.0	35.0	37.0	35.0	37.0
8	35.985780642907486	37.0	35.0	37.0	35.0	37.0
9	37.60754086973476	39.0	37.0	39.0	35.0	39.0
10	37.02788626951582	39.0	37.0	39.0	33.0	39.0
11	36.95410037838398	39.0	37.0	39.0	33.0	39.0
12	37.04635254500292	39.0	37.0	39.0	33.0	39.0
13	37.04220311684408	39.0	37.0	39.0	33.0	39.0
14	38.202775628253484	40.0	38.0	41.0	33.0	41.0
15	38.189238702811174	40.0	37.0	41.0	33.0	41.0
16	38.17229368121099	40.0	37.0	41.0	33.0	41.0
17	38.10548646612113	40.0	37.0	41.0	33.0	41.0
18	38.02030031054702	40.0	37.0	41.0	33.0	41.0
19	37.95365964127655	40.0	37.0	41.0	33.0	41.0
20	37.8910871583018	40.0	37.0	41.0	33.0	41.0
21	37.84923338146914	40.0	37.0	41.0	33.0	41.0
22	37.79405390803827	39.0	37.0	41.0	33.0	41.0
23	37.686788245114826	39.0	37.0	41.0	33.0	41.0
24	37.61607938954864	39.0	36.0	41.0	32.0	41.0
25	37.60806284870531	39.0	36.0	41.0	32.0	41.0
26	37.44585940689378	39.0	36.0	41.0	32.0	41.0
27	37.41692105525056	39.0	36.0	41.0	32.0	41.0
28	37.375688778670764	39.0	36.0	41.0	32.0	41.0
29	37.15007200060119	39.0	36.0	41.0	31.0	41.0
30	37.12222838306351	39.0	36.0	41.0	31.0	41.0
31	37.019435084704796	39.0	35.0	41.0	31.0	41.0
32	36.88619233604884	39.0	35.0	41.0	31.0	41.0
33	36.832363508592344	39.0	35.0	40.0	31.0	41.0
34	36.63032718129325	39.0	35.0	40.0	30.0	41.0
35	36.59370131835233	39.0	35.0	40.0	30.0	41.0
36	36.44253661469219	39.0	35.0	40.0	30.0	41.0
37	36.344008514147255	39.0	35.0	40.0	30.0	41.0
38	36.305798434875506	39.0	35.0	40.0	30.0	41.0
39	36.16809957002744	39.0	35.0	40.0	30.0	41.0
40	36.14410884784821	38.0	35.0	40.0	30.0	41.0
41	36.14874978927892	38.0	35.0	40.0	30.0	41.0
42	36.01305962949648	38.0	35.0	40.0	29.0	41.0
43	35.93270533373142	38.0	35.0	40.0	29.0	41.0
44	35.79286980788331	38.0	35.0	40.0	28.0	41.0
45	35.572417981261566	38.0	34.0	40.0	27.0	41.0
46	35.416882465365575	38.0	34.0	40.0	26.0	41.0
47	35.30156167171382	38.0	34.0	40.0	26.0	41.0
48	35.21678172545531	38.0	34.0	40.0	26.0	41.0
49	35.11656379415749	38.0	34.0	40.0	26.0	41.0
50	34.978038293352185	38.0	34.0	40.0	26.0	41.0
51	33.80368309986453	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	4.0
13	5.0
14	11.0
15	10.0
16	20.0
17	58.0
18	138.0
19	230.0
20	471.0
21	807.0
22	1230.0
23	1703.0
24	2315.0
25	2975.0
26	3791.0
27	4766.0
28	6029.0
29	7775.0
30	9999.0
31	12441.0
32	16061.0
33	20525.0
34	29116.0
35	40842.0
36	48289.0
37	65680.0
38	96898.0
39	120112.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.364723158196185	34.763596333554716	22.301500740316477	24.57017976793262
2	20.296248453865793	33.428995627156716	21.351783360447804	24.922972558529686
3	19.250462570858137	32.932607843495674	23.10660760383218	24.71032198181401
4	19.453770333315052	33.09204499986798	21.053422618140903	26.400762048676064
5	17.343716043440025	35.05444220352305	19.292708339680352	28.30913341335657
6	20.04846077135087	36.29886444185825	20.45832597078949	23.194348816001398
7	84.26832562551158	6.713218254234224	4.939505277674533	4.0789508425796726
8	86.00060525188024	5.637779091187898	3.7694193440938184	4.592196312838042
9	77.58110476747562	10.690819872572138	7.674106390281849	4.053968969670382
10	29.080728008335416	45.709921865638144	12.87642909514844	12.332921030878001
11	16.75613426842718	26.783005014653998	38.14792924646141	18.31293147045741
12	21.978970543731478	24.69569844645247	35.086126530139715	18.23920447967633
13	21.223218112060962	27.477216735011385	32.505072538828536	18.79449261409912
14	17.38657112623564	28.501676628950136	33.35242517116645	20.75932707364778
15	16.35743982516751	31.7527728863406	31.769630572937928	20.120156715553957
16	18.4299197533497	31.687779395844885	30.42812430817476	19.454176542630652
17	18.460994765992968	31.365452303917685	31.216170380435333	18.95738254965401
18	18.56742160667971	29.171109581056022	31.475535028444806	20.785933783819463
19	17.67376111236359	34.118129731069125	30.829052902670217	17.379056253897073
20	18.899091512865667	32.12607924737538	31.219826264275717	17.755002975483237
21	18.88568660545092	33.02745771868786	29.25621043267385	18.830645243187362
22	18.4077813456496	30.714298771013716	30.111890355981537	20.76602952735515
23	18.30866627264363	31.601459916280263	28.001226752133107	22.088647058943003
24	15.422345980660374	33.389390218885886	30.94319772035332	20.245066080100415
25	18.151869476822714	32.95291830927559	28.138119291489712	20.757092922411992
26	19.449098926185673	32.92407744786811	28.54067272324756	19.086150902698652
27	16.7742105829713	31.70930848957159	29.33034363277053	22.186137294686578
28	20.134374041599894	30.542269125857864	29.879132418143744	19.444224414398494
29	18.889545593949105	29.99510517774704	30.415734924049016	20.69961430425484
30	21.805112956655435	31.326659314278054	27.91531348188408	18.95291424718243
31	20.887689217376824	31.46862947007964	26.910148530436253	20.733532782107293
32	21.50918947024212	29.421740728780133	27.983353542246785	21.08571625873096
33	21.192752413391098	30.044662714250027	28.510410129235492	20.252174743123383
34	20.01433918884062	28.01239750831206	30.546534323671644	21.426728979175678
35	20.84280308800322	28.43749555708561	32.194322412395884	18.52537894251529
36	20.76582642269735	30.082033971285068	31.100603830147637	18.051535775869947
37	20.782684109294678	30.16957207879648	30.311542234598065	18.736201577310773
38	20.268423115747314	28.429574475431448	33.469007244743146	17.832995164078095
39	18.583873083961436	29.61733051424068	31.761709491283764	20.03708691051412
40	19.568118255655957	29.644952747701364	30.237612139159186	20.549316857483493
41	17.522854351618847	29.145112184857737	32.561535633696685	20.77049782982673
42	19.892679498818946	29.435145636194875	30.502257508271434	20.16991735671474
43	19.15825305621734	29.208887047406655	30.8558627174997	20.776997178876304
44	21.45719467784555	28.759416439697212	28.40093671868177	21.382452163775472
45	21.431603490962857	30.20409987062233	29.297034468891475	19.06726216952333
46	18.157759511898885	30.45838690218683	32.054180198514494	19.329673387399794
47	19.695464876096004	29.609815641902117	30.117780391057707	20.576939090944173
48	19.012017702601973	28.42469996364427	32.52538300460844	20.037899329145315
49	19.428991565063562	28.933274026773255	29.955905978791815	21.68182842937137
50	19.532778045198913	27.66732269471136	29.214370873167233	23.585528386922498
51	18.81439687056343	26.972501660380576	32.191682051844495	22.021419417211494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7942.0
1	5810.0
2	3678.0
3	2345.0
4	1012.0
5	897.0
6	782.0
7	942.0
8	1102.0
9	1423.0
10	1744.0
11	2148.0
12	2552.0
13	2870.0
14	3188.0
15	3100.0
16	3012.0
17	2966.5
18	2921.0
19	3079.5
20	3238.0
21	3006.5
22	2775.0
23	3337.5
24	3900.0
25	4589.0
26	6569.0
27	7860.0
28	9873.5
29	11887.0
30	13196.0
31	14505.0
32	16504.0
33	18503.0
34	19629.5
35	20756.0
36	22043.0
37	23330.0
38	24581.0
39	25832.0
40	26809.5
41	27787.0
42	27948.5
43	28110.0
44	33583.5
45	39057.0
46	42123.5
47	45190.0
48	47213.0
49	49236.0
50	44938.5
51	40641.0
52	35998.0
53	31355.0
54	26393.5
55	21432.0
56	17510.5
57	13589.0
58	11578.0
59	9567.0
60	8088.5
61	6610.0
62	5959.0
63	5308.0
64	4321.0
65	3334.0
66	2713.0
67	2092.0
68	1601.0
69	1110.0
70	933.0
71	756.0
72	617.5
73	479.0
74	448.0
75	321.0
76	225.0
77	184.0
78	143.0
79	101.0
80	59.0
81	46.5
82	34.0
83	24.0
84	14.0
85	12.0
86	10.0
87	7.0
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	492357.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.17800228695653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.77837507763519	24.062187242630383
2	9.79606370810247	6.304355208049863
3	3.8684723511793195	3.734391364898288
4	2.1971640837711486	2.8280140364962714
5	1.3916332720163043	2.238998930477272
6	1.022484499867948	1.9740905145077061
7	0.7815224492750196	1.7603481812055615
8	0.6046528287099355	1.5565216084034434
9	0.5133970214695335	1.4868081477866983
>10	4.294532289687655	28.768461876189306
>50	0.5313212543736396	11.914206331672641
>100	0.21404837184539954	9.959173654332137
>500	0.003166396033215969	0.7609018154252081
>1k	0.0025331168265727757	1.2436485751312387
>5k	6.332792066431939E-4	1.4078925127939912
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6909	1.4032500807341015	No Hit
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCT	2481	0.50390265599961	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1319	0.2678950436370357	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1178	0.2392572868873602	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGT	1125	0.228492740024007	No Hit
AATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCT	979	0.19883945998533584	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCC	923	0.18746559914858527	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	684	0.13892358593459625	No Hit
AATACAGGGATTGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGT	639	0.12978387633363594	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	509	0.10338027081975071	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6525752655085639	0.0
2	0.0	0.0	0.0	0.8946760176051117	0.0
3	0.0	0.0	0.0	1.1824753177064609	0.0
4	0.0	0.0	0.0	2.606848282851671	0.0
5	0.0	0.0	0.0	2.806703266126002	0.0
6	0.0	0.0	0.0	4.2083285095977105	0.0
7	0.0	0.0	0.0	5.144437877393842	0.0
8	0.0	0.0	0.0	5.947513694331552	0.0
9	0.0	0.0	0.0	7.502279849783795	0.0
10	0.0	0.0	0.0	9.709011956771205	0.0
11	0.0	0.0	0.0	11.843641910239928	0.0
12	0.0	0.0	0.0	12.751113521286383	0.0
13	0.0	0.0	0.0	13.123404359032167	0.0
14	0.0	0.0	0.0	13.500163499249528	0.0
15	0.0	0.0	0.0	13.815381928153759	0.0
16	0.0	0.0	0.0	14.388746377120667	0.0
17	0.0	0.0	0.0	15.219850636834655	0.0
18	0.0	0.0	0.0	16.156772423261984	0.0
19	0.0	0.0	0.0	16.60847718220722	0.0
20	0.0	0.0	0.0	17.060385045810257	0.0
21	0.0	0.0	0.0	17.58398885361638	0.0
22	0.0	0.0	0.0	18.17238304726042	0.0
23	0.0	0.0	0.0	18.717312844135453	0.0
24	0.0	0.0	0.0	19.131849450703452	0.0
25	0.0	0.0	0.0	19.4889074391143	0.0
26	0.0	0.0	0.0	19.82524875242964	0.0
27	0.0	0.0	0.0	20.192258869072646	0.0
28	0.0	0.0	0.0	20.543833031722915	0.0
29	0.0	0.0	0.0	20.947401986769762	0.0
30	0.0	0.0	0.0	21.458007096476745	0.0
31	0.0	0.0	0.0	21.843702841637267	0.0
32	0.0	0.0	0.0	22.193449062367346	0.0
33	0.0	0.0	0.0	22.555584667223172	0.0
34	0.0	0.0	0.0	22.930718970178145	0.0
35	0.0	0.0	0.0	23.312555726840483	0.0
36	0.0	0.0	0.0	23.694392483502824	0.0
37	0.0	0.0	0.0	24.069323681799993	0.0
38	0.0	0.0	0.0	24.432677914602614	0.0
39	0.0	0.0	0.0	24.78851727506667	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACGCT	45	3.8380676E-10	45.000004	40
CATACCG	65	0.0	45.000004	1
TGTAACG	45	3.8380676E-10	45.000004	1
CGATAGG	90	0.0	45.000004	2
AATCGCG	65	0.0	45.000004	1
GTGCATT	35	1.2100281E-7	45.0	10
TATTTCG	20	7.029355E-4	45.0	39
CTCACCG	25	3.8875456E-5	45.0	1
TCGCACG	35	1.2100281E-7	45.0	1
ACCGGAT	25	3.8875456E-5	45.0	4
GCAAGCG	50	2.1827873E-11	45.0	1
CTCGTCG	25	3.8875456E-5	45.0	1
CTCGTAG	20	7.029355E-4	45.0	1
GTCTGCG	25	3.8875456E-5	45.0	26
TTTACGG	80	0.0	45.0	2
TAGACCG	20	7.029355E-4	45.0	1
CCGTGCG	25	3.8875456E-5	45.0	1
TGCGGTA	25	3.8875456E-5	45.0	29
CACGAAC	20	7.029355E-4	45.0	18
AAGCGCG	20	7.029355E-4	45.0	1
>>END_MODULE