SRR3554190_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554190_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	179123
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7059	3.940867448624688	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1425	0.7955427276229183	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1281	0.7151510414631287	No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	928	0.5180797552519777	Illumina Single End Adapter 1 (95% over 22bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	928	0.5180797552519777	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	675	0.37683602887401396	No Hit
AACTTTGGGTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	591	0.3299408786141367	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT	423	0.23615057809438209	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGT	417	0.23280092450439085	No Hit
AACCCTGGGGTTTCCTTCCTTGGGTGGGATTTTTCCTTTATTATGTGCAAC	412	0.2300095465127315	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC	377	0.21046990057111592	No Hit
AAATCCGGGTGGATTCAAACATAATTTCTACCTTTTTTGTGTAATTTAATC	297	0.16580785270456613	No Hit
TTGTAAGGGATTTTTTTCCTTCATTTCATTCTTAGGGAGCTGTTTAGTTAG	275	0.15352578954126495	No Hit
TTCTGCGGGGACTTTTTTTGTAAGAAATTGTTTATAATATGTTGCATTTGT	259	0.14459337996795496	No Hit
TGAGTAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTA	237	0.1323113168046538	No Hit
CTTAGCGGGGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCA	234	0.13063649000965816	No Hit
GTATTTGGGATTTTCTTTTCTAATAAATGACAGATGATTTTACTTGCTAAT	223	0.12449545842800756	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	220	0.12282063163301196	No Hit
GAATATGGGAGAAAACTGAAAATCACTGAAAATGAGAAATACACACGTTAG	219	0.12226235603468008	No Hit
AAATAAGGGGAATATTTTGTTAATACTAAGGGACAATTATTTTAAGACCAT	207	0.1155630488546976	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	192	0.10718891487971952	No Hit
AAAGGCGGGAGAAACATAATTTTATAATATTTCTAGGTTGTTGTTGGGGTG	185	0.10328098569139643	No Hit
CAAACTGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTA	185	0.10328098569139643	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCT	30	2.1552132E-6	45.000004	33
CTCACTA	115	0.0	45.000004	45
AGCCGAA	30	2.1552132E-6	45.000004	30
TCGTTCA	30	2.1552132E-6	45.000004	16
CCGGGTG	55	1.8189894E-12	45.000004	5
CTCGTTC	30	2.1552132E-6	45.000004	15
GGGCTCA	45	3.8016879E-10	45.000004	7
CCGATCC	30	2.1552132E-6	45.000004	42
AGCCCGG	65	0.0	45.000004	2
GTTTATA	60	0.0	45.000004	30
TGGCGGG	45	3.8016879E-10	45.000004	3
GTCTCTA	30	2.1552132E-6	45.000004	41
TAAACGG	30	2.1552132E-6	45.000004	2
ATTAAGG	30	2.1552132E-6	45.000004	2
ATATGTT	60	0.0	45.000004	37
TTCAACA	30	2.1552132E-6	45.000004	24
TAACGTC	30	2.1552132E-6	45.000004	32
CCTCGTT	30	2.1552132E-6	45.000004	14
CGTTCAT	30	2.1552132E-6	45.000004	17
TCCTAAC	45	3.8016879E-10	45.000004	26

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content