##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554190_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	179123
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04790562909286	33.0	31.0	34.0	30.0	34.0
2	32.24867269976497	34.0	31.0	34.0	30.0	34.0
3	32.35884838909576	34.0	31.0	34.0	30.0	34.0
4	35.91272477571278	37.0	35.0	37.0	35.0	37.0
5	35.69472373732017	37.0	35.0	37.0	33.0	37.0
6	35.68445704906684	37.0	35.0	37.0	33.0	37.0
7	35.59901855149813	37.0	35.0	37.0	35.0	37.0
8	35.782797295713	37.0	35.0	37.0	35.0	37.0
9	37.40220407206221	39.0	37.0	39.0	35.0	39.0
10	37.05802158293463	39.0	37.0	39.0	33.0	39.0
11	37.062917659932005	39.0	37.0	39.0	33.0	39.0
12	37.147317764887816	39.0	37.0	39.0	33.0	39.0
13	37.130106128191244	39.0	37.0	39.0	33.0	39.0
14	38.34376378242884	40.0	38.0	41.0	33.0	41.0
15	38.33099601949498	40.0	38.0	41.0	33.0	41.0
16	38.29587490160392	40.0	38.0	41.0	33.0	41.0
17	38.22678829631035	40.0	38.0	41.0	33.0	41.0
18	38.01505669288701	40.0	37.0	41.0	33.0	41.0
19	37.805072492086445	40.0	36.0	41.0	33.0	41.0
20	37.752896054666344	39.0	36.0	41.0	33.0	41.0
21	37.67339760946389	39.0	36.0	41.0	33.0	41.0
22	37.629807450746135	39.0	36.0	41.0	33.0	41.0
23	37.505261747514275	39.0	36.0	41.0	33.0	41.0
24	37.45489970578876	39.0	35.0	41.0	33.0	41.0
25	37.36487218280176	39.0	35.0	41.0	32.0	41.0
26	37.26041881835387	39.0	35.0	41.0	32.0	41.0
27	37.18764201135532	39.0	35.0	41.0	32.0	41.0
28	37.12293786950866	39.0	35.0	41.0	32.0	41.0
29	36.95719142711991	39.0	35.0	41.0	31.0	41.0
30	36.90470235536475	39.0	35.0	41.0	31.0	41.0
31	36.85485950994568	39.0	35.0	41.0	31.0	41.0
32	36.74228881829804	39.0	35.0	40.0	31.0	41.0
33	36.55303339046353	39.0	35.0	40.0	31.0	41.0
34	36.483483416423354	39.0	35.0	40.0	31.0	41.0
35	36.43415976731073	38.0	35.0	40.0	31.0	41.0
36	36.331928339744195	38.0	35.0	40.0	30.0	41.0
37	36.25725897846731	38.0	35.0	40.0	30.0	41.0
38	36.21237920311741	38.0	35.0	40.0	30.0	41.0
39	36.11276608810706	38.0	35.0	40.0	30.0	41.0
40	36.076494922483434	38.0	35.0	40.0	30.0	41.0
41	35.97544703918536	38.0	35.0	40.0	30.0	41.0
42	35.8542844860794	38.0	35.0	40.0	29.0	41.0
43	35.793203552865904	38.0	35.0	40.0	29.0	41.0
44	35.751450120866664	38.0	35.0	40.0	29.0	41.0
45	35.59134226202107	38.0	35.0	40.0	28.0	41.0
46	35.47738704688957	38.0	35.0	40.0	28.0	41.0
47	35.35547082172585	38.0	34.0	40.0	27.0	41.0
48	35.29279880305712	38.0	34.0	40.0	27.0	41.0
49	35.10885257616275	37.0	34.0	40.0	27.0	41.0
50	34.94409428158305	37.0	34.0	40.0	26.0	41.0
51	34.07371471000374	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	0.0
12	1.0
13	2.0
14	4.0
15	4.0
16	19.0
17	31.0
18	80.0
19	169.0
20	263.0
21	406.0
22	653.0
23	753.0
24	1048.0
25	1135.0
26	1297.0
27	1543.0
28	1940.0
29	2511.0
30	3253.0
31	4057.0
32	5586.0
33	7530.0
34	10829.0
35	18387.0
36	18063.0
37	23352.0
38	33151.0
39	43033.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.287562177944764	38.80685339124512	21.521524315693686	22.384060115116426
2	18.674318764201136	39.24342490914064	20.114111532299034	21.968144794359183
3	19.345924308994377	37.76008664437286	20.925844252273578	21.968144794359183
4	19.118706140473307	35.904936831116046	20.53281823104794	24.443538797362706
5	16.55845424652334	39.349497272823704	17.8486291542683	26.243419326384664
6	17.59573030822396	42.14813284726138	19.398960490835908	20.857176353678756
7	77.88502872327953	13.116685182807345	4.56446129196139	4.433824801951731
8	80.10640732904206	11.69922343864272	3.6667541298437385	4.527615102471486
9	70.825633782373	18.34772753917699	6.930991553290197	3.895647125159806
10	24.325184370516347	51.083333798563004	12.135236680939913	12.45624514998074
11	16.792929997822725	34.0771425221775	33.857181936434735	15.272745543565037
12	22.620768968809145	29.277088927720058	30.541583157941748	17.56055894552905
13	20.680202989007554	32.970082010685395	27.085857204267462	19.263857796039595
14	15.033245311880664	35.88037270478945	29.26815651814675	19.818225465183144
15	14.226537072291107	38.30608017954143	29.120771760187136	18.346610987980327
16	16.45517326083194	35.71233174969155	28.58315235899354	19.249342630482964
17	14.599465171976798	38.665609664867155	27.943368523305217	18.79155663985083
18	15.65013984803738	37.948225521010706	28.17393634541628	18.227698285535638
19	16.60981560156987	40.00826247885531	27.08920685785745	16.292715061717367
20	17.563908599119042	37.5138871055085	27.60840316430609	17.313801131066363
21	16.176593737264337	41.2883884258303	25.404889377690193	17.13012845921518
22	15.257672102410075	38.73036963427366	26.357307548444364	19.654650714871906
23	15.211893503346863	41.10136610038912	24.190639951318367	19.496100444945654
24	14.247751545027718	41.352590119638464	25.109003310574295	19.290655024759523
25	13.90831998124194	44.12498674095454	24.048837949342072	17.91785532846145
26	16.444566024463636	40.78426556053662	23.1980259374843	19.57314247751545
27	14.72786855959313	40.83730174237814	24.514998073949183	19.919831624079542
28	16.992234386427203	38.52380766289086	27.819431340475536	16.664526610206394
29	15.172814211463631	41.63563584799272	23.348202073435573	19.843347867108076
30	17.27137218559314	43.05868034814067	22.914421933531706	16.755525532734488
31	19.754582046973308	40.94281583046286	21.48356157500712	17.819040547556707
32	18.956247941358733	38.87775439223327	23.20472524466428	18.96127242174372
33	16.163195122904373	42.744371186279814	22.98699776131485	18.105435929500956
34	16.119091350636154	40.96458857879781	23.599426092684915	19.316893977881122
35	16.739893815981198	41.71658580975084	23.343177593050584	18.200342781217376
36	20.385433473088323	41.20520536167885	22.630259653980783	15.779101511252044
37	17.007307827582164	43.520932543559454	23.850650111934257	15.621109516924125
38	17.730833003020273	40.48893776901906	26.111108009580008	15.669121218380667
39	16.60981560156987	42.55009127806033	23.70549845636797	17.13459466400183
40	19.575375579908776	41.57031760298789	22.875342641648476	15.978964175454854
41	15.919786962031676	40.87024000267972	25.984937724357003	17.225035310931595
42	16.232421297097524	42.27821106167271	23.421336176817046	18.068031464412723
43	15.677495352355644	41.08908403722582	25.494213473423287	17.73920713699525
44	18.896512452337223	37.16943106133774	24.351423323637945	19.58263316268709
45	17.653790970450473	41.47820212926313	24.074518626865338	16.793488273421055
46	16.243028533465832	40.79766417489658	25.727014397927682	17.232292893709907
47	16.321187117232295	40.67484354326357	24.54235357826745	18.46161576123669
48	17.22280220853827	37.78911697548612	26.404202698704243	18.58387811727137
49	16.51993323023844	40.43311020918587	23.596634714693256	19.45032184588244
50	16.032558632894713	38.340134991039676	24.183940644138385	21.443365731927223
51	16.351892275140546	36.0004019584308	26.69283118304182	20.954874583386836
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7983.0
1	5980.5
2	3978.0
3	2481.5
4	985.0
5	823.0
6	661.0
7	706.5
8	752.0
9	997.0
10	1242.0
11	1426.5
12	1611.0
13	2090.0
14	2569.0
15	2395.0
16	2221.0
17	2269.0
18	2317.0
19	2518.0
20	2719.0
21	2434.5
22	2150.0
23	2542.0
24	2934.0
25	2920.0
26	2948.5
27	2991.0
28	3782.5
29	4574.0
30	4812.0
31	5050.0
32	6133.5
33	7217.0
34	7708.5
35	8200.0
36	8633.0
37	9066.0
38	8691.5
39	8317.0
40	8807.5
41	9298.0
42	9008.5
43	8719.0
44	10761.0
45	12803.0
46	13132.5
47	13462.0
48	13870.0
49	14278.0
50	13405.0
51	12532.0
52	10262.5
53	7993.0
54	6714.0
55	5435.0
56	4586.5
57	3738.0
58	3266.0
59	2794.0
60	2478.0
61	2162.0
62	2062.5
63	1963.0
64	1671.5
65	1380.0
66	1145.0
67	910.0
68	716.0
69	522.0
70	372.5
71	223.0
72	192.0
73	161.0
74	133.5
75	93.0
76	80.0
77	69.5
78	59.0
79	45.0
80	31.0
81	20.0
82	9.0
83	13.0
84	17.0
85	10.0
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	179123.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.44629109606248
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.48823234852279	25.248013934558934
2	9.459188783174762	6.327495631493443
3	4.179602737439493	4.193766294669026
4	2.381906192622267	3.1866371152783284
5	1.5673510265398098	2.6211039341681412
6	1.0398931730929728	2.0868341865645395
7	0.71440494074445	1.6725936926022902
8	0.5658487731597396	1.5140434226760382
9	0.4590218661325321	1.3817321058713845
>10	3.4651977966950422	23.779190835347777
>50	0.45735269571023196	10.468784019919273
>100	0.21031547320981472	10.325307191147983
>500	0.006676681689200468	1.742936417992106
>1k	0.003338340844600234	1.5106937690860471
>5k	0.001669170422300117	3.940867448624688
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7059	3.940867448624688	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1425	0.7955427276229183	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1281	0.7151510414631287	No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	928	0.5180797552519777	Illumina Single End Adapter 1 (95% over 22bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	928	0.5180797552519777	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	675	0.37683602887401396	No Hit
AACTTTGGGTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	591	0.3299408786141367	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT	423	0.23615057809438209	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGT	417	0.23280092450439085	No Hit
AACCCTGGGGTTTCCTTCCTTGGGTGGGATTTTTCCTTTATTATGTGCAAC	412	0.2300095465127315	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC	377	0.21046990057111592	No Hit
AAATCCGGGTGGATTCAAACATAATTTCTACCTTTTTTGTGTAATTTAATC	297	0.16580785270456613	No Hit
TTGTAAGGGATTTTTTTCCTTCATTTCATTCTTAGGGAGCTGTTTAGTTAG	275	0.15352578954126495	No Hit
TTCTGCGGGGACTTTTTTTGTAAGAAATTGTTTATAATATGTTGCATTTGT	259	0.14459337996795496	No Hit
TGAGTAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTA	237	0.1323113168046538	No Hit
CTTAGCGGGGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCA	234	0.13063649000965816	No Hit
GTATTTGGGATTTTCTTTTCTAATAAATGACAGATGATTTTACTTGCTAAT	223	0.12449545842800756	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	220	0.12282063163301196	No Hit
GAATATGGGAGAAAACTGAAAATCACTGAAAATGAGAAATACACACGTTAG	219	0.12226235603468008	No Hit
AAATAAGGGGAATATTTTGTTAATACTAAGGGACAATTATTTTAAGACCAT	207	0.1155630488546976	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	192	0.10718891487971952	No Hit
AAAGGCGGGAGAAACATAATTTTATAATATTTCTAGGTTGTTGTTGGGGTG	185	0.10328098569139643	No Hit
CAAACTGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTA	185	0.10328098569139643	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6291765993200203	0.0
2	0.0	0.0	0.0	0.8446709802761231	0.0
3	0.0	0.0	0.0	1.125483606237055	0.0
4	0.0	0.0	0.0	2.4111923091953575	0.0
5	0.0	0.0	0.0	2.597098083439871	0.0
6	0.0	0.0	0.0	3.7276061700619127	0.0
7	0.0	0.0	0.0	4.566136118756385	0.0
8	0.0	0.0	0.0	5.401316413860867	0.0
9	0.0	0.0	0.0	6.616124115831021	0.0
10	0.0	0.0	0.0	8.3568274314298	0.0
11	0.0	0.0	0.0	9.927814965135688	0.0
12	0.0	0.0	0.0	10.68874460566203	0.0
13	0.0	0.0	0.0	11.047157539791094	0.0
14	0.0	0.0	0.0	11.378773245200225	0.0
15	0.0	0.0	0.0	11.636696571629551	0.0
16	0.0	0.0	0.0	12.115697034998297	0.0
17	0.0	0.0	0.0	12.791210508979862	0.0
18	0.0	0.0	0.0	13.752561089307347	0.0
19	0.0	0.0	0.0	14.145028834934655	0.0
20	0.0	0.0	0.0	14.574342770051864	0.0
21	0.0	0.0	0.0	15.009239461152392	0.0
22	0.0	0.0	0.0	15.52117818482272	0.0
23	0.0	0.0	0.0	15.98622175823317	0.0
24	0.0	0.0	0.0	16.292156786119037	0.0
25	0.0	0.0	0.0	16.583018372849942	0.0
26	0.0	0.0	0.0	16.845407904065922	0.0
27	0.0	0.0	0.0	17.207170491784975	0.0
28	0.0	0.0	0.0	17.499706905310877	0.0
29	0.0	0.0	0.0	17.81680744516338	0.0
30	0.0	0.0	0.0	18.263986199427208	0.0
31	0.0	0.0	0.0	18.54424054978981	0.0
32	0.0	0.0	0.0	18.82840282934073	0.0
33	0.0	0.0	0.0	19.118706140473307	0.0
34	0.0	0.0	0.0	19.419616687974184	0.0
35	0.0	0.0	0.0	19.752348944579982	0.0
36	0.0	0.0	0.0	20.032603294942582	0.0
37	0.0	0.0	0.0	20.308949716116857	0.0
38	0.0	0.0	0.0	20.594786822462776	0.0
39	0.0	0.0	0.0	20.89067288957867	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCT	30	2.1552132E-6	45.000004	33
CTCACTA	115	0.0	45.000004	45
AGCCGAA	30	2.1552132E-6	45.000004	30
TCGTTCA	30	2.1552132E-6	45.000004	16
CCGGGTG	55	1.8189894E-12	45.000004	5
CTCGTTC	30	2.1552132E-6	45.000004	15
GGGCTCA	45	3.8016879E-10	45.000004	7
CCGATCC	30	2.1552132E-6	45.000004	42
AGCCCGG	65	0.0	45.000004	2
GTTTATA	60	0.0	45.000004	30
TGGCGGG	45	3.8016879E-10	45.000004	3
GTCTCTA	30	2.1552132E-6	45.000004	41
TAAACGG	30	2.1552132E-6	45.000004	2
ATTAAGG	30	2.1552132E-6	45.000004	2
ATATGTT	60	0.0	45.000004	37
TTCAACA	30	2.1552132E-6	45.000004	24
TAACGTC	30	2.1552132E-6	45.000004	32
CCTCGTT	30	2.1552132E-6	45.000004	14
CGTTCAT	30	2.1552132E-6	45.000004	17
TCCTAAC	45	3.8016879E-10	45.000004	26
>>END_MODULE