SRR3554189_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554189_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	175362
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6838	3.8993624616507563	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1331	0.759001380002509	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1237	0.705397976756652	No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	982	0.5599844892279969	Illumina Single End Adapter 1 (95% over 22bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	845	0.48186038024201366	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	639	0.36438909227768845	No Hit
AACTTTGGGTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	566	0.32276091741654406	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT	400	0.22809958828024315	No Hit
AACCCTGGGGTTTCCTTCCTTGGGTGGGATTTTTCCTTTATTATGTGCAAC	398	0.22695909033884193	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC	394	0.2246780944560395	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGT	392	0.22353759651463828	No Hit
AAATCCGGGTGGATTCAAACATAATTTCTACCTTTTTTGTGTAATTTAATC	294	0.16765319738597873	No Hit
TTCTGCGGGGACTTTTTTTGTAAGAAATTGTTTATAATATGTTGCATTTGT	293	0.16708294841527813	No Hit
TTGTAAGGGATTTTTTTCCTTCATTTCATTCTTAGGGAGCTGTTTAGTTAG	280	0.1596697117961702	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	248	0.14142174473375074	No Hit
TGAGTAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTA	242	0.1380002509095471	No Hit
CTTAGCGGGGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCA	228	0.1300167653197386	No Hit
GTATTTGGGATTTTCTTTTCTAATAAATGACAGATGATTTTACTTGCTAAT	205	0.11690103899362461	No Hit
AAATAAGGGGAATATTTTGTTAATACTAAGGGACAATTATTTTAAGACCAT	204	0.116330790022924	No Hit
AATCAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTAT	191	0.1089175534038161	No Hit
TCATGGGGGGTAGACAAAGCCACCTTGACCCGATTCTTCGCTTTCCACTTC	187	0.10663655752101368	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	185	0.10549605957961246	No Hit
GGAATAGGGATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCT	177	0.1009340678140076	No Hit
ATGTTAGGGATTCTTTAAATGGAATGACTGATAATGCTTTTTTTTTTAAGA	176	0.100363818843307	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAG	20	7.0169254E-4	45.0	1
GTTGAGT	25	3.87725E-5	45.0	21
TATTTCG	20	7.0169254E-4	45.0	31
CTTCGCT	20	7.0169254E-4	45.0	36
GTGCAAC	45	3.8016879E-10	45.0	45
TGTCACG	25	3.87725E-5	45.0	1
AGCAACG	40	6.7575456E-9	45.0	1
TCGTTGT	20	7.0169254E-4	45.0	26
AATCCCT	25	3.87725E-5	45.0	25
GTCGCGG	25	3.87725E-5	45.0	2
ACACGTT	20	7.0169254E-4	45.0	43
GAAGCGG	45	3.8016879E-10	45.0	2
AAATCCC	25	3.87725E-5	45.0	24
ACACGCG	20	7.0169254E-4	45.0	1
CATATCC	25	3.87725E-5	45.0	28
ATGATAG	20	7.0169254E-4	45.0	1
CTCGTCC	40	6.7575456E-9	45.0	37
CTACTAA	20	7.0169254E-4	45.0	25
CACGGTT	20	7.0169254E-4	45.0	13
GGTAACG	20	7.0169254E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content