##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554189_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	175362
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12389799386412	33.0	31.0	34.0	30.0	34.0
2	32.315404705694505	34.0	31.0	34.0	30.0	34.0
3	32.411183722813384	34.0	31.0	34.0	30.0	34.0
4	35.953707188558525	37.0	35.0	37.0	35.0	37.0
5	35.76693924567466	37.0	35.0	37.0	35.0	37.0
6	35.74761350805762	37.0	35.0	37.0	33.0	37.0
7	35.62084716187087	37.0	35.0	37.0	35.0	37.0
8	35.821648931923676	37.0	35.0	37.0	35.0	37.0
9	37.45124941549481	39.0	37.0	39.0	35.0	39.0
10	37.13249164585258	39.0	37.0	39.0	34.0	39.0
11	37.14922275065293	39.0	37.0	39.0	34.0	39.0
12	37.250384918055225	39.0	37.0	39.0	34.0	39.0
13	37.22558479031945	39.0	37.0	39.0	34.0	39.0
14	38.46016810939656	40.0	38.0	41.0	34.0	41.0
15	38.44306064027555	40.0	38.0	41.0	34.0	41.0
16	38.429665491953784	40.0	38.0	41.0	34.0	41.0
17	38.337062761601715	40.0	38.0	41.0	34.0	41.0
18	38.154936645339355	40.0	37.0	41.0	33.0	41.0
19	37.91719984945427	40.0	36.0	41.0	33.0	41.0
20	37.84549674387838	40.0	36.0	41.0	33.0	41.0
21	37.79080986758819	39.0	36.0	41.0	33.0	41.0
22	37.74899921305642	39.0	36.0	41.0	33.0	41.0
23	37.64022422189528	39.0	36.0	41.0	33.0	41.0
24	37.613855909490084	39.0	36.0	41.0	33.0	41.0
25	37.51957664716415	39.0	35.0	41.0	33.0	41.0
26	37.43624616507567	39.0	35.0	41.0	33.0	41.0
27	37.393848154104084	39.0	35.0	41.0	33.0	41.0
28	37.27857232467695	39.0	35.0	41.0	32.0	41.0
29	37.18546777523066	39.0	35.0	41.0	32.0	41.0
30	37.10445250396323	39.0	35.0	41.0	32.0	41.0
31	36.99276354056181	39.0	35.0	41.0	32.0	41.0
32	36.89700733340176	39.0	35.0	41.0	31.0	41.0
33	36.71213261710063	39.0	35.0	41.0	31.0	41.0
34	36.65721193873245	39.0	35.0	40.0	31.0	41.0
35	36.551567614420456	39.0	35.0	40.0	31.0	41.0
36	36.48545294875743	39.0	35.0	40.0	31.0	41.0
37	36.39621468733249	38.0	35.0	40.0	30.0	41.0
38	36.380527138148516	38.0	35.0	40.0	31.0	41.0
39	36.280419931342024	38.0	35.0	40.0	30.0	41.0
40	36.22555057538121	38.0	35.0	40.0	30.0	41.0
41	36.16237269191729	38.0	35.0	40.0	30.0	41.0
42	36.06687879928377	38.0	35.0	40.0	30.0	41.0
43	35.9922674239573	38.0	35.0	40.0	30.0	41.0
44	35.959951414787696	38.0	35.0	40.0	30.0	41.0
45	35.81628859159909	38.0	35.0	40.0	29.0	41.0
46	35.62698304079561	38.0	35.0	40.0	28.0	41.0
47	35.55010207456576	38.0	35.0	40.0	28.0	41.0
48	35.4780226046692	38.0	35.0	40.0	28.0	41.0
49	35.37131191478199	38.0	34.0	40.0	27.0	41.0
50	35.229565128134944	38.0	34.0	40.0	27.0	41.0
51	34.371545716859984	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	4.0
14	8.0
15	8.0
16	14.0
17	29.0
18	60.0
19	138.0
20	245.0
21	383.0
22	559.0
23	730.0
24	940.0
25	1054.0
26	1227.0
27	1489.0
28	1719.0
29	2216.0
30	2911.0
31	3837.0
32	5008.0
33	6774.0
34	10136.0
35	17702.0
36	17263.0
37	22691.0
38	33393.0
39	44786.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.187303976916322	38.506061746558544	21.65235341750208	22.65428085902305
2	18.738951426192678	39.0227073140133	20.02771409997605	22.210627159817978
3	19.412415460590093	37.566861691814644	21.066707724592558	21.954015123002705
4	19.16264641142323	35.77057743410773	20.454830579030805	24.611945575438238
5	16.39009591587687	39.44640229924385	17.857346517489535	26.30615526738974
6	17.765536433206737	41.79753880544246	19.498523055165887	20.93840170618492
7	77.97926574742533	12.95377561843501	4.611603426055816	4.455355208083849
8	80.23916241831184	11.534425930361197	3.633626441304273	4.592785210022695
9	70.87510407043716	18.17896693696468	7.038583045357603	3.9073459472405654
10	24.458548602319773	50.90327436958977	12.127484859889828	12.510692168200634
11	16.901038993624617	33.90757404682885	33.8625243781435	15.32886258140304
12	22.57102450930076	29.123185182650747	30.79743616062773	17.508354147420764
13	20.745657554088115	32.97749797561615	27.247066069045744	19.029778401249985
14	15.14866390666165	35.53962660097398	29.479020540367923	19.83268895199644
15	14.059488372623488	37.949498751154756	29.15340837809788	18.83760449812388
16	16.420319111324	35.59722174701474	28.664134761236753	19.318324380424492
17	14.621753857734287	38.391441703447725	28.036860893466088	18.9499435453519
18	15.720623624274358	37.42429944913949	28.328258117494098	18.52681880909205
19	16.65982367901826	39.845576578734274	27.208289139038104	16.286310603209362
20	17.405709332694656	37.53036575768981	27.719232216785848	17.34469269282969
21	16.34219500233802	40.95813232057116	25.41485612618469	17.284816550906125
22	15.179457351079481	38.674855441885924	26.388271119170632	19.75741608786396
23	15.121862205038719	40.868032983200465	24.23215976095163	19.777945050809183
24	14.23056306383367	41.1765376763495	25.181624297168142	19.41127496264869
25	13.810859821398022	43.81564991275191	24.327961588029336	18.045528677820734
26	16.502434963104893	40.62567717065271	23.49140634801154	19.38048151823086
27	14.71755568481199	40.62681766859411	24.536102462335055	20.119524184258847
28	16.946658911280664	38.428507886543265	27.69414126207502	16.93069194010105
29	15.108176229741904	41.59053842907813	23.495398090806447	19.805887250373512
30	17.182171736180017	42.90553255551374	23.03805841630456	16.874237292001688
31	19.726052394475428	40.750561695236144	21.640378189117367	17.88300772117106
32	18.994993214037247	38.80031021544006	23.19886862604213	19.00582794448056
33	16.2258642123151	42.43450690571503	23.03577742042176	18.303851461548113
34	16.216740228783888	40.66844584345525	23.69441498158096	19.4203989461799
35	16.76189824477367	41.581414445546926	23.541588257433197	18.11509905224621
36	20.30029310797094	41.12407477104504	22.773462893899477	15.802169227084544
37	17.12799808396346	43.47350053033154	23.671034773782235	15.727466611922766
38	17.8254125751303	40.40727181487437	25.819732895382124	15.947582714613201
39	16.69061712343609	42.33072159304753	23.66020004333892	17.31846124017746
40	19.66389525666906	41.34190987785267	22.75863642066126	16.23555844481701
41	15.871739601510019	40.73459472405652	25.990807586592307	17.402858087841153
42	16.43343483765012	42.0216466509278	23.587208175089245	17.957710336332845
43	15.878012340187725	40.809867588189	25.36638496367514	17.94573510794813
44	18.994422965066548	36.98862923552423	24.56005291910448	19.45689488030474
45	17.742156225408014	41.10354580809982	23.98866345046247	17.165634516029698
46	16.20818649422338	40.75911542979665	25.81460065464582	17.218097421334157
47	16.353029732781334	40.643354888744426	24.41863117437073	18.58498420410351
48	17.325304227825868	37.69174621639808	26.603254981124756	18.379694574651293
49	16.55375737046795	40.041171975684584	23.596902407591156	19.808168246256315
50	15.998334873005554	38.191854563702506	24.24926723007265	21.560543333219286
51	16.41005462985139	35.88177598339435	26.613519462597367	21.094649924156887
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7679.0
1	5765.0
2	3851.0
3	2411.5
4	972.0
5	803.5
6	635.0
7	674.5
8	714.0
9	976.0
10	1238.0
11	1401.5
12	1565.0
13	1982.0
14	2399.0
15	2274.5
16	2150.0
17	2222.0
18	2294.0
19	2496.5
20	2699.0
21	2389.5
22	2080.0
23	2495.5
24	2911.0
25	2920.0
26	2901.0
27	2873.0
28	3718.0
29	4563.0
30	4674.0
31	4785.0
32	5797.0
33	6809.0
34	7385.0
35	7961.0
36	8455.0
37	8949.0
38	8465.0
39	7981.0
40	8388.5
41	8796.0
42	8602.5
43	8409.0
44	10482.0
45	12555.0
46	12943.0
47	13331.0
48	13857.5
49	14384.0
50	13371.5
51	12359.0
52	10312.0
53	8265.0
54	6848.0
55	5431.0
56	4545.5
57	3660.0
58	3176.5
59	2693.0
60	2459.5
61	2226.0
62	2041.0
63	1856.0
64	1569.0
65	1282.0
66	1057.0
67	832.0
68	676.5
69	521.0
70	389.0
71	257.0
72	206.5
73	156.0
74	130.5
75	94.5
76	84.0
77	69.5
78	55.0
79	50.0
80	45.0
81	25.5
82	6.0
83	10.0
84	14.0
85	7.5
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	175362.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.29170515847219
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.88737774275877	24.931284999030577
2	9.794282386392833	6.521367228932151
3	4.110926500059951	4.105792589044377
4	2.4579914698275123	3.273229091821489
5	1.6426577139822887	2.734343814509415
6	1.11337592709957	2.223970985732371
7	0.8050564395950737	1.8761191136050002
8	0.580668368133468	1.5465152085400486
9	0.4230828522978366	1.2676634618674514
>10	3.5165550435929496	24.18425884741278
>50	0.4641921172984361	10.75147409358926
>100	0.1918432366694644	9.491223868340917
>500	0.006851544166766585	1.728994879164243
>1k	0.0034257720833832926	1.464399356759161
>5k	0.0017128860416916463	3.8993624616507563
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6838	3.8993624616507563	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1331	0.759001380002509	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1237	0.705397976756652	No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	982	0.5599844892279969	Illumina Single End Adapter 1 (95% over 22bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	845	0.48186038024201366	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	639	0.36438909227768845	No Hit
AACTTTGGGTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	566	0.32276091741654406	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT	400	0.22809958828024315	No Hit
AACCCTGGGGTTTCCTTCCTTGGGTGGGATTTTTCCTTTATTATGTGCAAC	398	0.22695909033884193	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC	394	0.2246780944560395	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGT	392	0.22353759651463828	No Hit
AAATCCGGGTGGATTCAAACATAATTTCTACCTTTTTTGTGTAATTTAATC	294	0.16765319738597873	No Hit
TTCTGCGGGGACTTTTTTTGTAAGAAATTGTTTATAATATGTTGCATTTGT	293	0.16708294841527813	No Hit
TTGTAAGGGATTTTTTTCCTTCATTTCATTCTTAGGGAGCTGTTTAGTTAG	280	0.1596697117961702	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	248	0.14142174473375074	No Hit
TGAGTAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTA	242	0.1380002509095471	No Hit
CTTAGCGGGGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCA	228	0.1300167653197386	No Hit
GTATTTGGGATTTTCTTTTCTAATAAATGACAGATGATTTTACTTGCTAAT	205	0.11690103899362461	No Hit
AAATAAGGGGAATATTTTGTTAATACTAAGGGACAATTATTTTAAGACCAT	204	0.116330790022924	No Hit
AATCAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTAT	191	0.1089175534038161	No Hit
TCATGGGGGGTAGACAAAGCCACCTTGACCCGATTCTTCGCTTTCCACTTC	187	0.10663655752101368	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	185	0.10549605957961246	No Hit
GGAATAGGGATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCT	177	0.1009340678140076	No Hit
ATGTTAGGGATTCTTTAAATGGAATGACTGATAATGCTTTTTTTTTTAAGA	176	0.100363818843307	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6346871043897766	0.0
2	0.0	0.0	0.0	0.8622164436993192	0.0
3	0.0	0.0	0.0	1.1233904722801975	0.0
4	0.0	0.0	0.0	2.4771615287234408	0.0
5	0.0	0.0	0.0	2.6602114483183357	0.0
6	0.0	0.0	0.0	3.8223788506061744	0.0
7	0.0	0.0	0.0	4.687446539158997	0.0
8	0.0	0.0	0.0	5.538828252415004	0.0
9	0.0	0.0	0.0	6.785392502366533	0.0
10	0.0	0.0	0.0	8.610189208608478	0.0
11	0.0	0.0	0.0	10.263340974669541	0.0
12	0.0	0.0	0.0	11.044011815558672	0.0
13	0.0	0.0	0.0	11.365632235033816	0.0
14	0.0	0.0	0.0	11.704360123629977	0.0
15	0.0	0.0	0.0	11.987203613097478	0.0
16	0.0	0.0	0.0	12.505559927464331	0.0
17	0.0	0.0	0.0	13.224073630547096	0.0
18	0.0	0.0	0.0	14.22999281486297	0.0
19	0.0	0.0	0.0	14.628026596411994	0.0
20	0.0	0.0	0.0	15.06483730796866	0.0
21	0.0	0.0	0.0	15.536433206738062	0.0
22	0.0	0.0	0.0	16.052508525222112	0.0
23	0.0	0.0	0.0	16.523534175020814	0.0
24	0.0	0.0	0.0	16.84002235375965	0.0
25	0.0	0.0	0.0	17.133130324699763	0.0
26	0.0	0.0	0.0	17.396015100192745	0.0
27	0.0	0.0	0.0	17.751850457909924	0.0
28	0.0	0.0	0.0	18.06890888561946	0.0
29	0.0	0.0	0.0	18.399653288625814	0.0
30	0.0	0.0	0.0	18.82734001665127	0.0
31	0.0	0.0	0.0	19.114745497884375	0.0
32	0.0	0.0	0.0	19.38903525279137	0.0
33	0.0	0.0	0.0	19.658763015932756	0.0
34	0.0	0.0	0.0	19.938185011576053	0.0
35	5.702489707006079E-4	0.0	0.0	20.247830202666485	0.0
36	5.702489707006079E-4	0.0	0.0	20.5437894184601	0.0
37	5.702489707006079E-4	0.0	0.0	20.86255859308174	0.0
38	5.702489707006079E-4	0.0	0.0	21.16136905372886	0.0
39	5.702489707006079E-4	0.0	0.0	21.47272499173139	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAG	20	7.0169254E-4	45.0	1
GTTGAGT	25	3.87725E-5	45.0	21
TATTTCG	20	7.0169254E-4	45.0	31
CTTCGCT	20	7.0169254E-4	45.0	36
GTGCAAC	45	3.8016879E-10	45.0	45
TGTCACG	25	3.87725E-5	45.0	1
AGCAACG	40	6.7575456E-9	45.0	1
TCGTTGT	20	7.0169254E-4	45.0	26
AATCCCT	25	3.87725E-5	45.0	25
GTCGCGG	25	3.87725E-5	45.0	2
ACACGTT	20	7.0169254E-4	45.0	43
GAAGCGG	45	3.8016879E-10	45.0	2
AAATCCC	25	3.87725E-5	45.0	24
ACACGCG	20	7.0169254E-4	45.0	1
CATATCC	25	3.87725E-5	45.0	28
ATGATAG	20	7.0169254E-4	45.0	1
CTCGTCC	40	6.7575456E-9	45.0	37
CTACTAA	20	7.0169254E-4	45.0	25
CACGGTT	20	7.0169254E-4	45.0	13
GGTAACG	20	7.0169254E-4	45.0	1
>>END_MODULE