FastQCFastQC Report
Sat 18 Jun 2016
SRR3554183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330877
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52511.5869945629342626No Hit
CTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGCT12910.390175201056586No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10350.3128050604907564No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8130.245710641718826No Hit
TCAAAGGGGGTTTTCCATGCTTTGTTTTTGTTGTAGTTGTGTGCGTGTTTT5580.1686427282645817No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA5280.15957591491702353No Hit
ATCAATGGGGCTATTTGTGCTTATATTTTTCTCAATATGTACAATCATAAA5270.15927368780543827No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4940.14930019312312431No Hit
AACTTTGGGTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4670.14114006111032196No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGC207.0259965E-445.014
ATCTAGT207.0259965E-445.039
CTATCCG207.0259965E-445.01
ACGCAGT207.0259965E-445.031
GCAACGC207.0259965E-445.028
CAGCACG207.0259965E-445.01
TAGCGCG207.0259965E-445.01
CAACCCG207.0259965E-445.01
GGATCGC207.0259965E-445.08
CGTCTAG406.7902874E-945.01
CGACAGC207.0259965E-445.038
GCGTTAA207.0259965E-445.016
TCTAGCG207.0259965E-445.01
TAACACG207.0259965E-445.01
CTGCCCG406.7902874E-945.01
TGTTACC207.0259965E-445.026
TACTCCA207.0259965E-445.040
TCCAACG207.0259965E-445.01
ATTGGCG207.0259965E-445.032
CGTAAGG351.2084274E-745.02