##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554183_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	330877
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.171949092865326	33.0	31.0	34.0	30.0	34.0
2	32.35469071588536	34.0	31.0	34.0	30.0	34.0
3	32.46126203997256	34.0	31.0	34.0	30.0	34.0
4	35.98497024574086	37.0	35.0	37.0	35.0	37.0
5	35.81549034837719	37.0	35.0	37.0	35.0	37.0
6	35.80412056443935	37.0	35.0	37.0	35.0	37.0
7	35.882920239242985	37.0	35.0	37.0	35.0	37.0
8	36.050885978777615	37.0	35.0	37.0	35.0	37.0
9	37.67072053965673	39.0	38.0	39.0	35.0	39.0
10	37.16629140133645	39.0	37.0	39.0	34.0	39.0
11	37.136954215614864	39.0	37.0	39.0	34.0	39.0
12	37.18754098955201	39.0	37.0	39.0	34.0	39.0
13	37.17134161637104	39.0	37.0	39.0	33.0	39.0
14	38.3924086594112	40.0	38.0	41.0	34.0	41.0
15	38.4010825775137	40.0	38.0	41.0	34.0	41.0
16	38.374275032716085	40.0	38.0	41.0	34.0	41.0
17	38.31788247596539	40.0	38.0	41.0	34.0	41.0
18	38.197321058882906	40.0	37.0	41.0	33.0	41.0
19	38.14773465668511	40.0	37.0	41.0	34.0	41.0
20	38.07734898466802	40.0	37.0	41.0	33.0	41.0
21	38.02000441251583	40.0	37.0	41.0	33.0	41.0
22	37.973122942966725	40.0	37.0	41.0	33.0	41.0
23	37.8733910184147	40.0	37.0	41.0	33.0	41.0
24	37.86190034363223	40.0	37.0	41.0	33.0	41.0
25	37.830819307476794	40.0	37.0	41.0	33.0	41.0
26	37.73478059822835	40.0	36.0	41.0	33.0	41.0
27	37.737403929556905	40.0	36.0	41.0	33.0	41.0
28	37.65088235205227	40.0	36.0	41.0	33.0	41.0
29	37.58142451726775	40.0	36.0	41.0	33.0	41.0
30	37.52517702953061	40.0	36.0	41.0	33.0	41.0
31	37.40752001499047	39.0	36.0	41.0	32.0	41.0
32	37.36405371180227	39.0	36.0	41.0	32.0	41.0
33	37.23686445416273	39.0	35.0	41.0	32.0	41.0
34	37.198007114426204	39.0	35.0	41.0	32.0	41.0
35	37.12616470773127	39.0	35.0	41.0	31.0	41.0
36	37.067571937608236	39.0	35.0	41.0	31.0	41.0
37	37.004630119349486	39.0	35.0	41.0	31.0	41.0
38	36.95937463166071	39.0	35.0	41.0	31.0	41.0
39	36.88236414135766	39.0	35.0	41.0	31.0	41.0
40	36.80303859137988	39.0	35.0	41.0	31.0	41.0
41	36.721089710073535	39.0	35.0	41.0	31.0	41.0
42	36.654524188746876	39.0	35.0	41.0	31.0	41.0
43	36.56982806299622	39.0	35.0	41.0	30.0	41.0
44	36.497904054981156	39.0	35.0	40.0	30.0	41.0
45	36.36980811600685	39.0	35.0	40.0	30.0	41.0
46	36.2513109100965	39.0	35.0	40.0	30.0	41.0
47	36.15523895586578	38.0	35.0	40.0	30.0	41.0
48	36.07266748670956	38.0	35.0	40.0	29.0	41.0
49	35.97187776726699	38.0	35.0	40.0	29.0	41.0
50	35.88140608141394	38.0	35.0	40.0	29.0	41.0
51	34.89441393629657	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	3.0
14	7.0
15	16.0
16	20.0
17	43.0
18	82.0
19	148.0
20	247.0
21	424.0
22	645.0
23	872.0
24	1221.0
25	1534.0
26	1918.0
27	2499.0
28	3184.0
29	4080.0
30	5337.0
31	6937.0
32	9166.0
33	12265.0
34	18384.0
35	26175.0
36	29268.0
37	40383.0
38	67171.0
39	98802.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.002831868035557	34.08154691924794	20.281252550041255	26.634368662675257
2	21.164057942981835	33.436292036013384	20.81831012732828	24.5813398936765
3	19.847254417804805	34.183699682963756	21.25805057468485	24.710995324546584
4	19.384846937079338	33.3824956101512	20.251936520217484	26.98072093255198
5	17.26260815952756	36.193207747894235	17.62951187299208	28.91467221958613
6	20.305430718968076	38.71499076696174	19.63660212102987	21.342976393040313
7	84.81822550373704	7.495534594426327	4.169223004318826	3.5170168975178084
8	86.58323183539503	6.493651719521151	3.784185664159189	3.1389307809246336
9	77.48619577667834	12.810802805876504	6.304759774780357	3.398241642664797
10	29.215388195613475	47.178558799795695	12.79750481296675	10.808548191624077
11	19.595499233854273	29.860945305959618	34.27587895199727	16.267676508188845
12	23.133974256294636	26.904559700432486	32.41506662596675	17.54639941730613
13	21.957404110893172	27.84660160724378	30.567854519957567	19.62813976190548
14	17.800572418149343	30.99278583884646	31.41197484261523	19.794666900388965
15	17.855275525346276	33.0418856553946	30.71957252997337	18.383266289285746
16	20.519709741082035	32.28148224264606	29.860038624624863	17.338769391647048
17	19.496973195477473	32.613932065389854	29.857016353509007	18.03207838562366
18	19.44801240340066	32.27997110708814	29.1204888825757	19.15152760693551
19	19.45828812519456	33.55808956198224	29.944359988757153	17.039262324066044
20	21.32333163078727	32.781365885208096	28.545652916340515	17.349649567664116
21	20.715250682277706	33.67595813550051	27.852343922363897	17.756447259857893
22	18.683075583978336	32.43017798154601	29.679609039008454	19.207137395467196
23	18.453080752061947	33.0234498015879	29.773601670711475	18.74986777563868
24	17.59687134494087	32.80856632525077	30.515569229653316	19.078993100155042
25	18.713902749360035	34.35959586190639	27.56976157303167	19.356739815701907
26	18.419835769787564	34.96949017308547	27.90342030422181	18.70725375290516
27	18.185609758308978	32.65926613212765	28.82249295055262	20.33263115901075
28	18.268722213994927	32.69916011085691	29.40760463858171	19.62451303656646
29	18.22308592014555	33.512453268132866	28.948219428972095	19.31624138274948
30	19.645971161489012	32.98808922953243	27.66012747939567	19.7058121295829
31	19.634486531248772	34.829256793309895	26.06769282845287	19.468563846988456
32	21.152875539853177	32.78015697676176	27.51928964539693	18.547677837988136
33	18.48149010055096	34.98641489133424	26.531006990513088	20.001088017601706
34	17.893960595629192	33.103539986158	28.56620435992831	20.436295058284497
35	18.46668097208328	32.58068708311548	30.019010085318715	18.93362185948253
36	18.91488377856424	34.35536468234419	28.540515055443567	18.189236483648003
37	18.93331963237094	35.047464767874466	27.656500754056644	18.362714845697948
38	18.272348939333952	33.71887438534561	30.68723422903375	17.321542446286685
39	18.96354234352947	33.964282799952855	27.429528193256104	19.642646663261573
40	20.966401412005066	31.856248696645583	28.36764114761679	18.809708743732564
41	16.890868812277674	33.109886755501286	29.795059795634028	20.204184636587012
42	19.39572711309641	32.27634438174911	27.98955503102361	20.33837347413087
43	19.441363406945783	32.461911828262465	27.504782744040835	20.591942020750913
44	19.781973361702384	31.630787271402966	28.23677680830037	20.35046255859428
45	19.548049577335387	33.32265464205732	27.813356624969398	19.315939155637896
46	17.8670623826981	32.21106332564669	30.215759934960722	19.70611435669448
47	18.07046122879499	33.95491375949371	27.77376487335173	20.200860138359573
48	19.03637907742152	31.060786938953143	30.811147344783713	19.091686638841622
49	18.479374510769865	32.50482807810757	28.65566358495755	20.36013382616501
50	17.562719681331735	31.468793539593264	28.909232131577596	22.059254647497408
51	18.094639397721814	30.666682785445953	29.361968344732333	21.876709472099904
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6067.0
1	4515.5
2	2964.0
3	1934.5
4	905.0
5	791.0
6	677.0
7	735.5
8	794.0
9	955.0
10	1116.0
11	1457.5
12	1799.0
13	1850.0
14	1901.0
15	1989.5
16	2078.0
17	2168.0
18	2258.0
19	2238.5
20	2219.0
21	2491.0
22	2763.0
23	3376.5
24	3990.0
25	4495.0
26	5466.5
27	5933.0
28	7445.0
29	8957.0
30	9992.5
31	11028.0
32	12294.0
33	13560.0
34	14879.0
35	16198.0
36	17321.5
37	18445.0
38	19637.5
39	20830.0
40	19827.5
41	18825.0
42	19894.5
43	20964.0
44	21420.0
45	21876.0
46	23097.5
47	24319.0
48	24820.0
49	25321.0
50	23935.0
51	22549.0
52	20027.0
53	17505.0
54	15218.0
55	12931.0
56	11392.0
57	9853.0
58	8744.5
59	7636.0
60	7295.0
61	6954.0
62	6147.0
63	5340.0
64	4121.0
65	2902.0
66	2379.0
67	1856.0
68	1419.0
69	982.0
70	781.0
71	580.0
72	486.5
73	393.0
74	320.5
75	211.5
76	175.0
77	153.5
78	132.0
79	78.5
80	25.0
81	17.0
82	9.0
83	6.0
84	3.0
85	6.0
86	9.0
87	5.5
88	2.0
89	1.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	330877.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.26277164620293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.56899999999493	22.566646188855525
2	9.378999999999362	5.676690705394359
3	3.686999999999749	3.3473651717862785
4	2.0629999999998594	2.4972839162444957
5	1.3870000000066813	2.098723213674283
6	0.9669999999999599	1.7558460109126213
7	0.7959999999999459	1.6862416361263128
8	0.629999999999957	1.5252436909685239
9	0.5479999999999626	1.492559897590627
>10	5.117999999999651	31.881224673839693
>50	0.6339999999999568	13.499919803653745
>100	0.21499999999998534	8.945070043184053
>500	0.003999999999999728	0.7341748401368331
>1k	0.001999999999999864	0.7039120684906323
>5k	9.99999999999932E-4	1.5890981391420078
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5251	1.5869945629342626	No Hit
CTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGCT	1291	0.390175201056586	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1035	0.3128050604907564	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	813	0.245710641718826	No Hit
TCAAAGGGGGTTTTCCATGCTTTGTTTTTGTTGTAGTTGTGTGCGTGTTTT	558	0.1686427282645817	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	528	0.15957591491702353	No Hit
ATCAATGGGGCTATTTGTGCTTATATTTTTCTCAATATGTACAATCATAAA	527	0.15927368780543827	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	494	0.14930019312312431	No Hit
AACTTTGGGTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	467	0.14114006111032196	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4518295318199814	0.0
2	0.0	0.0	0.0	0.6153343991876136	0.0
3	0.0	0.0	0.0	0.7966706661387767	0.0
4	0.0	0.0	0.0	1.5948524678354796	0.0
5	0.0	0.0	0.0	1.7021430924482512	0.0
6	0.0	0.0	0.0	2.4359505193772915	0.0
7	0.0	0.0	0.0	2.9896305878015093	0.0
8	0.0	0.0	0.0	3.4753095561190412	0.0
9	0.0	0.0	0.0	4.286487123613911	0.0
10	0.0	0.0	0.0	5.491164390392805	0.0
11	0.0	0.0	0.0	6.561955046739423	0.0
12	0.0	0.0	0.0	7.076950044880726	0.0
13	0.0	0.0	0.0	7.282766707870296	0.0
14	0.0	0.0	0.0	7.488583370859867	0.0
15	0.0	0.0	0.0	7.683822084943952	0.0
16	0.0	0.0	0.0	8.096966546481019	0.0
17	0.0	0.0	0.0	8.628584035759513	0.0
18	0.0	0.0	0.0	9.277163417221505	0.0
19	0.0	0.0	0.0	9.603568697733598	0.0
20	0.0	0.0	0.0	9.930578432468863	0.0
21	0.0	0.0	0.0	10.30987345750838	0.0
22	0.0	0.0	0.0	10.741151545740562	0.0
23	0.0	0.0	0.0	11.126491113011785	0.0
24	0.0	0.0	0.0	11.435367221051932	0.0
25	0.0	0.0	0.0	11.7007226250238	0.0
26	0.0	0.0	0.0	11.95187335475116	0.0
27	0.0	0.0	0.0	12.242011381873022	0.0
28	0.0	0.0	0.0	12.536380588557076	0.0
29	0.0	0.0	0.0	12.83528320191491	0.0
30	0.0	0.0	0.0	13.199164644263579	0.0
31	0.0	0.0	0.0	13.49444053228239	0.0
32	0.0	0.0	0.0	13.788205284743274	0.0
33	0.0	0.0	0.0	14.106450433242564	0.0
34	0.0	0.0	0.0	14.399006277257108	0.0
35	0.0	0.0	0.0	14.732362781335663	0.0
36	0.0	0.0	0.0	15.05665247206666	0.0
37	0.0	0.0	0.0	15.375804301900706	0.0
38	0.0	0.0	0.0	15.723063253112183	0.0
39	0.0	0.0	0.0	16.083015743010243	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGC	20	7.0259965E-4	45.0	14
ATCTAGT	20	7.0259965E-4	45.0	39
CTATCCG	20	7.0259965E-4	45.0	1
ACGCAGT	20	7.0259965E-4	45.0	31
GCAACGC	20	7.0259965E-4	45.0	28
CAGCACG	20	7.0259965E-4	45.0	1
TAGCGCG	20	7.0259965E-4	45.0	1
CAACCCG	20	7.0259965E-4	45.0	1
GGATCGC	20	7.0259965E-4	45.0	8
CGTCTAG	40	6.7902874E-9	45.0	1
CGACAGC	20	7.0259965E-4	45.0	38
GCGTTAA	20	7.0259965E-4	45.0	16
TCTAGCG	20	7.0259965E-4	45.0	1
TAACACG	20	7.0259965E-4	45.0	1
CTGCCCG	40	6.7902874E-9	45.0	1
TGTTACC	20	7.0259965E-4	45.0	26
TACTCCA	20	7.0259965E-4	45.0	40
TCCAACG	20	7.0259965E-4	45.0	1
ATTGGCG	20	7.0259965E-4	45.0	32
CGTAAGG	35	1.2084274E-7	45.0	2
>>END_MODULE