SRR3554177_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554177_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	407437
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5124	1.257617742129458	No Hit
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	1182	0.2901062004678024	RNA PCR Primer, Index 40 (96% over 26bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1082	0.2655625286854164	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	844	0.20714858984333773	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCC	574	0.14088067603089557	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGT	571	0.140144365877424	No Hit
AAAGGCGGGAGATTTTTCTTTTCTATTTTATGAAAGAATAGAAACTAGTGA	542	0.13302670106053208	No Hit
AATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	482	0.11830049799110047	TruSeq Adapter, Index 19 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	469	0.1151098206593903	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTA	20	7.0279185E-4	45.000004	15
TAATACG	40	6.7957444E-9	45.000004	1
TCCATAT	20	7.0279185E-4	45.000004	22
AATTTCG	20	7.0279185E-4	45.000004	31
TTGGACG	20	7.0279185E-4	45.000004	1
CGACGCT	20	7.0279185E-4	45.000004	23
TTCGTAC	20	7.0279185E-4	45.000004	16
CGACAAC	20	7.0279185E-4	45.000004	25
CCTAAGT	20	7.0279185E-4	45.000004	43
CATAGGA	20	7.0279185E-4	45.000004	37
CGAATTG	20	7.0279185E-4	45.000004	1
TTAGTAC	20	7.0279185E-4	45.000004	25
TAGGCAT	20	7.0279185E-4	45.000004	23
TACAACG	20	7.0279185E-4	45.000004	1
TAATCCG	20	7.0279185E-4	45.000004	1
TAGAACG	25	3.8863574E-5	45.0	1
TGTATCG	25	3.8863574E-5	45.0	29
TACGATG	25	3.8863574E-5	45.0	1
ACGGAAC	35	1.2093551E-7	45.0	42
GTATCGA	25	3.8863574E-5	45.0	30